AUTHOR=Tan Kian-Hin , How Kah-Yan , Tan Jia-Yi , Yin Wai-Fong , Chan Kok-Gan 

TITLE=Cloning and Characterization of the Autoinducer Synthase Gene from Lipid-Degrading Bacterium Cedecea neteri

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00072

DOI=10.3389/fmicb.2017.00072

ISSN=1664-302X

ABSTRACT=<p>The process of intercellular communication among bacteria, termed quorum sensing (QS), is mediated by small diffusible molecules known as the autoinducers. QS allows the population to react to the change of cell density in unison, in processes such as biofilm formation, plasmid conjugation, virulence, motility and root nodulation. In Gram-negative proteobacteria, <italic>N</italic>-acyl homoserine lactone (AHL) is the common “language” to coordinate gene expression. This signaling molecule is usually synthesized by LuxI-type proteins. We have previously discovered that a rare bacterium, <italic>Cedecea neteri</italic>, exhibits AHL-type QS activity. With information generated from genome sequencing, we have identified the <italic>luxIR</italic> gene pair responsible for AHL-type QS and named it <italic>cneIR</italic>. In this study, we have cloned and expressed the 636 bp <italic>luxI</italic> homolog in an <italic>Escherichia coli</italic> host for further characterization. Our findings show that <italic>E. coli</italic> harboring <italic>cneI</italic> produced the same AHL profile as the wild type <italic>C. neteri</italic>, with the synthesis of AHL known as <italic>N</italic>-butyryl-homoserine lactone. This 25 kDa LuxI homolog shares high similarity with other AHL synthases from closely related species. This work is the first documentation of molecular cloning and characterization of <italic>luxI</italic> homolog from <italic>C. neteri</italic>.</p>