AUTHOR=Nazina Tamara N. , Shestakova Natalya M. , Semenova Ekaterina M. , Korshunova Alena V. , Kostrukova Nadezda K. , Tourova Tatiana P. , Min Liu , Feng Qingxian , Poltaraus Andrey B. TITLE=Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir JOURNAL=Frontiers in Microbiology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00707 DOI=10.3389/fmicb.2017.00707 ISSN=1664-302X ABSTRACT=
The goal of this work was to study the overall genomic diversity of microorganisms of the Dagang high-temperature oilfield (PRC) and to characterize the metabolically active fraction of these populations. At this water-flooded oilfield, the microbial community of formation water from the near-bottom zone of an injection well where the most active microbial processes of oil degradation occur was investigated using molecular, cultural, radiotracer, and physicochemical techniques. The samples of microbial DNA and RNA from back-flushed water were used to obtain the clone libraries for the 16S rRNA gene and cDNA of 16S rRNA, respectively. The DNA-derived clone libraries were found to contain bacterial and archaeal 16S rRNA genes and the