AUTHOR=Kohl Claudia , Wegener Merle , Nitsche Andreas , Kurth Andreas 

TITLE=Use of RNALater® Preservation for Virome Sequencing in Outbreak Settings

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01888

DOI=10.3389/fmicb.2017.01888

ISSN=1664-302X

ABSTRACT=<p>Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of <italic>RNALater<sup>®</sup></italic>-treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in <italic>RNALater<sup>®</sup></italic> buffer yield similar results to those stored snap-frozen.</p>