In Vivo Study of the Sorbicillinoid Gene Cluster in Trichoderma reesei

Sorbicillinoids are a diverse group of yellow secondary metabolites that are produced by a range of not closely related ascomycetes, including Penicillium chrysogenum, Acremonium chrysogenum, and Trichoderma reesei. They share a similarity to the name-giving compound sorbicillin, a hexaketide. Previously, a conserved gene cluster containing two polyketide synthases has been identified as the source of sorbicillin, and a model for the biosynthesis of sorbicillin in P. chrysogenum has been proposed. In this study, we deleted the major genes of interest of the cluster in T. reesei, namely sor1, sor3, and sor4. Sor1 is the homolog of P. chrysogenum SorA, which is the first polyketide synthase of the proposed biosynthesis pathway. Sor3 is a flavin adenine dinucleotide (FAD)-dependent monooxygenase, and its homolog in P. chrysogenum, SorC, was shown to oxidize sorbicillin and 2′,3′-dihydrosorbicillin to sorbicillinol and 2′,3′-dihydrosorbicillinol, respectively, in vitro. Sor4 is an FAD/flavin mononucleotide-containing dehydrogenase with an unknown function. We measured the amounts of synthesized sorbicillinoids throughout growth and could verify the roles of Sor1 and Sor3 in vivo in T. reesei. In the absence of Sor4, two compounds annotated to dihydrosorbicillinol accumulate in the supernatant and only small amounts of sorbicillinol are synthesized. Therefore, we suggest extending the current biosynthesis model about Sor4 reducing 2′,3′-dihydrosorbicillin and 2′,3′-dihydrosorbicillinol to sorbicillinol and sorbicillinol, respectively. Sorbicillinol turned out to be the main chemical building block for most sorbicillinoids, including oxosorbicillinol, bisorbicillinol, and bisvertinolon. Further, we detected the sorbicillinol-dependent synthesis of 5-hydroxyvertinolide at early time points, which contradicts previous models for biosynthesis of 5-hydroxyvertinolide. Finally, we investigated whether sorbicillinoids from T. reesei have a growth limiting effect on the fungus itself or on plant pathogenic fungi or on pathogenic bacteria.

Sorbicillinoids are a diverse group of yellow secondary metabolites that are produced by a range of not closely related ascomycetes, including Penicillium chrysogenum, Acremonium chrysogenum, and Trichoderma reesei. They share a similarity to the name-giving compound sorbicillin, a hexaketide. Previously, a conserved gene cluster containing two polyketide synthases has been identified as the source of sorbicillin, and a model for the biosynthesis of sorbicillin in P. chrysogenum has been proposed. In this study, we deleted the major genes of interest of the cluster in T. reesei, namely sor1, sor3, and sor4. Sor1 is the homolog of P. chrysogenum SorA, which is the first polyketide synthase of the proposed biosynthesis pathway. Sor3 is a flavin adenine dinucleotide (FAD)-dependent monooxygenase, and its homolog in P. chrysogenum, SorC, was shown to oxidize sorbicillin and 2 ,3 -dihydrosorbicillin to sorbicillinol and 2 ,3 -dihydrosorbicillinol, respectively, in vitro. Sor4 is an FAD/flavin mononucleotidecontaining dehydrogenase with an unknown function. We measured the amounts of synthesized sorbicillinoids throughout growth and could verify the roles of Sor1 and Sor3 in vivo in T. reesei. In the absence of Sor4, two compounds annotated to dihydrosorbicillinol accumulate in the supernatant and only small amounts of sorbicillinol are synthesized. Therefore, we suggest extending the current biosynthesis model about Sor4 reducing 2 ,3 -dihydrosorbicillin and 2 ,3 -dihydrosorbicillinol to sorbicillinol and sorbicillinol, respectively. Sorbicillinol turned out to be the main chemical building block for most sorbicillinoids, including oxosorbicillinol, bisorbicillinol, and bisvertinolon. Further, we detected the sorbicillinol-dependent synthesis of 5-hydroxyvertinolide at early time points, which contradicts previous models for biosynthesis of 5-hydroxyvertinolide. Finally, we investigated whether sorbicillinoids from T. reesei have a growth limiting effect on the fungus itself or on plant pathogenic fungi or on pathogenic bacteria.

INTRODUCTION
Sorbicillinoids are a group of yellow secondary metabolites that are produced by a range of ascomycetes, including Penicillium (Cram, 1948) and Trichoderma (Abe et al., 2000). Many of these hexaketide metabolites are highly oxygenated and have complex bicyclic and tricyclic frameworks (Harned and Volp, 2011). However, their name-giving, common feature is an apparent similarity of their core structures to sorbicillin (Figure 1, compound 1). Many sorbicillinoids possess interesting bioactive properties. For instance, bisorbicillinoids have been demonstrated to have outstanding radical scavenging properties . Additionally, trichodimerol was shown to inhibit the tumor necrosis factor-a (TNF-a) via targeting the prostaglandin H synthase-2, and thus to act anti-inflammatorily (Mazzucco and Warr, 1996;Warr et al., 1996). Further, some sorbicillinoids have been demonstrated to show antimicrobial activity (Maskey et al., 2005;Reategui et al., 2006). Zhao et al. (2017) observed anti-HIV and anti-inflammatory activities of sorbicillinoids. For detailed reviews about sorbicillinoids and their bioactive properties refer to (Meng et al., 2016) and (Harned and Volp, 2011).
In P. chrysogenum, the genes encoding for the two PKS, SorA and SorB, and the FAD-dependent monooxygenase SorC are part of a gene cluster (Salo et al., 2016), which is also present in a range of not closely related ascomycetes, including T. reesei and Acremonium chrysogenum (Martinez et al., 2008;Terfehr et al., 2014;Derntl et al., 2016; Figure 2). The core set of the gene cluster consists of genes encoding for two PKS, an FAD-dependent monooxygenase, a transporter, and two Gal4-like transcription factors. Additionally, some auxiliary genes can be present (Figure 2). P. chrysogenum Pcg21g05110, T. reesei protein ID 73631 (sor4), and A. chrysogenum ACRE_048110 are FAD/flavin mononucleotide (FMN)-containing dehydrogenases containing a "berberine and berberine like" domain according to a NCBI conserved domain search (Marchler-Bauer et al., 2015). Despite the similar domain architecture, only T. reesei sor4 and A. chrysogenum ACRE_048110 appear to be orthologous according to a DELTA-BLAST analysis (Boratyn et al., 2012). Further, the hydrolase ACRE_048140 is only present in A. chrysogenum; ACRE_048140 is related to Monascus ruber ctnB/citA which is considered to support the respective PKS in the citrinin biosynthesis pathway (He and Cox, 2016).
Recently, we verified that the PKS SorA is essential for the sorbicillinoid biosynthesis in P. chrysogenum (Salo et al., 2016). In T. reesei, which is studied and industrially applied for its outstanding protein secretion capabilities of plant cell wall-degrading enzymes (Peterson and Nevalainen, 2012), we identified the main regulator of the sorbicillinoid gene cluster FIGURE 1 | Currently suggested sorbicillinol biosynthesis pathway. The iterative PKS SorA combines three acetyl units, and the growing chain is modified by the ketoacyl reductase subunit, removing the keto-groups. The enoyl reductase subunit might be active in the second cycle, which inserts a single bond. The polyketide is handed over to the PKS SorB, which adds three more acetyl units and two methyl groups. SorB releases an aldehyde, which undergoes spontaneous cyclization, resulting in the formation of sorbicillin (1) or 2 ,3 -dihydrosorbicillin (2), depending on whether the enoyl reductase subunit had been active in SorA or not. Finally, the FAD-dependent monooxygenase SorC transforms sorbicillin or 2 ,3 -dihydrosorbicillin to sorbicillinol (3) or 2 ,3 -dihydrosorbicillinol (4). (Derntl et al., 2016). The deletion of this transcription factor, Yellow pigment regulator 1 (Ypr1, Figure 2), abolishes the synthesis of the yellow sorbicillinoids and the expression of all genes of cluster, except of sor4.
In this study, we deleted the cluster genes encoding for the first PKS, the FAD-dependent monooxygenase, and the FAD/FMNcontaining dehydrogenase in T. reesei. Further, we expressed the citA/ctnB-similar hydrolase from A. chrysogenum in T. reesei. The strains were investigated together with a ypr1 deletion strain and a ypr1 overexpression strain regarding their growth and their yellow pigment synthesis behavior. Further, the presence and the abundance of sorbicillinoids in their supernatants were measured, allowing us to extend the existing model for the biosynthesis pathway of sorbicillinoids. Additionally, we assayed the influence of the sorbicillinoids on the growth of other fungi and bacteria and on the confrontation behavior of T. reesei in presence of plant pathogenic fungi.

Fungal Strains and Cultivation Conditions
All T. reesei strains ( Table 1) were maintained on malt extract agar at 30 • C. Uridine was added to a final concentration of FIGURE 2 | Genomic organization of the sorbicillin cluster in P. chrysogenum, T. reesei, and A. chrysogenum. The arrows indicate the genes belonging to the sorbicillin cluster in the depicted fungi. Arrows with the same filling (color/pattern) represent homologs. The FCD Pc21g05110 shares only the same domain architecture with homologs FCDs sor4 and ACRE_048110, which is indicated by the distinction between dark and light blue. Orientation, distance, and size are to scale and comparable. The beginning of a protein IDs is given in brackets after the species name and the variable part of the ID in brackets above the respective arrow. FCD, FAD/FMN-containing dehydrogenase; MFS, transporter of the multifacilitator superfamily; TF, transcription factor; PKS, polyketide synthase; FMO, FAD-dependent monooxygenase; HYD, hydrolase. 5 mM if applicable. Mandels-Andreotti (MA) medium (Mandels, 1985) without peptone containing 1% (w/v) D-glucose was used as minimal medium for selections after fungal transformations. For cultivation on D-glucose, T. reesei was grown in 300 ml MA medium containing 1% (w/v) D-glucose 30 • C on a rotary shaker at 180 rpm. Mycelia and supernatants were separated by filtration through Miracloth (EMD Millipore, part of Merck KGaA, Darmstadt, Germany). Mycelia were dried at 80 • C overnight for biomass determination and supernatants were stored at −20 • C. Mycelia for RNA isolation and transcript analysis were harvested after 36 h cultivation and stored over liquid nitrogen. Fusarium oxysporum Foc4 (TUCIM 4812), Alternaria alternata TUCIM 3737, Rhizoctonia solani TUCIM 3753, and Thanatephorus cucumeris (Botrytis cinerea) TUCIM 4679 were maintained on potato dextrose agar at room temperature (approximately 22 • C).

Bacterial Strains and Cultivation Conditions
Escherichia coli Top10 (Invitrogen, part of Thermo Fisher Scientific Inc., Waltham, MA, United States) was used for all cloning purposes throughout this study and maintained on LB at 37 • C. If applicable, ampicillin was added to a final concentration

Plasmid Constructions
Polymerase chain reactions (PCRs) for cloning purposes were performed with Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, United States) according to the manufacturer's instructions. All used primers are listed in Supplementary Table S1. PCR products were cloned into EcoRV-digested pJET1.2 (Thermo Scientific, part of Thermo Fisher Scientific Inc., United States), and after verification of the PCR products by sequencing (Microsynth, Balgach, Switzerland), they were released for subsequent cloning purposes by digestion with suitable restriction endonucleases.
For the construction of the plasmids pCD sor1, pCD sor3, and pCD sor4 the 5 -and 3 -flanking regions of the respective genes were amplified by PCR using chromosomal DNA of T. reesei QM6a as template and corresponding primers given in Supplementary Table S1. Consecutively, corresponding flanking regions were inserted into pJET-pyr4 (Derntl et al., 2016) using the restriction enzymes indicated in the primer names.
For the construction of pRP4-ACRE_048140 ex the promoter of sor3 was amplified by PCR using chromosomal DNA of T. reesei QM6a as template and the primers P sor3 _fwd-BspEI and P sor3 _rev-SpeI and inserted into pCD-RPyr4T (Derntl et al., 2015) using BspEI and SpeI. The coding sequence of ACRE_048140 was amplified by PCR using chromosomal DNA of A. chrysogenum ATCC 11550 as template and the primers ACRE_048140_fwd-SpeI and ACRE-048140_rev-BamHI and subsequently inserted into the latter plasmid using SpeI and BamHI.

Fungal Transformation
The protoplast transformation of T. reesei was performed as described earlier (Gruber et al., 1990). Typically, 30 µg of linearized plasmid DNA of the plasmids pCD sor1, pCD sor3, pCD sor4, or pRP4-ACRE_048140 ex (in 15 µl sterile ddH 2 O) was used for the transformation of 10 7 protoplasts of the strain pyr4. For selection for prototrophy, 100 µl to 2 ml of the transformation reaction were added to 20 ml melted, 50 • C warm minimal medium agar containing 1.2 M sorbitol. This mixture was poured into sterile petri dishes. The plates were incubated at 30 • C for 3-5 days until colonies were visible. Candidates were subjected to homokaryon selection by spore streak-outs on selection medium plates to obtain stable, homokaryotic strains.

Genotype Testing
Chromosomal DNA was isolated from mycelium by grinding in liquid nitrogen followed by a phenol/chloroform extraction (Gruber et al., 1990). RNA was degraded using RNaseA (Thermo Scientific). DNA was precipitated with isopropanol, washed with 70% ethanol, and dissolved in ddH 2 O. For testing the genotype, 10 ng of chromosomal DNA were used as template in a 25-µl PCR using GoTaq R G2 polymerase (Promega, Madison, WI, United States) according to the manufacturer's instructions. All used primers are listed in Supplementary Table S1. For subsequent agarose gel electrophoresis of DNA fragments a GeneRuler 1 kb DNA Ladder (Thermo Scientific, United States) was applied for estimation of the fragment size.

RNA Isolation and RT-PCR
Approximately 20 mg of harvested mycelia were homogenized in 1 ml of peqGOLD TriFast DNA/RNA/protein purification system reagent (PEQLAB Biotechnologie, Erlangen, Germany) using a FastPrep FP120 BIO101 ThermoSavant cell disrupter (Qbiogene, Carlsbad, CA, United States). RNA was isolated according to the manufacturer's instructions, and the concentration was measured using the NanoDrop 1000 (Thermo Scientific). Synthesis of cDNA from mRNA was carried out using the RevertAid TM H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer's instructions.
Reverse transcription polymerase chain reactions (RT-PCRs) were performed in a Mastercycler R ep realplex 2.2 system (Eppendorf, Hamburg, Germany). All reactions were performed in triplicates. The amplification mixture (final volume 25 µl) contained 12.5 µl 2 × iQ SYBR Green Mix (Bio-Rad), 100 nM forward and reverse primer, and 2.5 µl cDNA (diluted 1:100) as template. All used primers are listed in Supplementary Table S1. Cycling conditions and control reactions were performed as described earlier (Steiger et al., 2010).

Metabolite Analysis
Culture supernatants were filtered using a 2 µm-pore polytetrafluoroethylene (PTFE) syringe filter. Liquid chromatography-mass spectrometry (LC-MS) analysis was performed in an Accella 1250 LC system coupled with the benchtop ES-MS Orbitrap Exactive (Thermo Fisher Scientific, United States) as described earlier (Salo et al., 2016). Reserpine is used as an internal standard. The differential analysis was done using the Thermo Scientific 205 SIEVE software. The response ratio indicates the fold-change of the compound detected in relation to reserpine. The metabolite analysis was performed from single representative biological samples in technical duplicates. Reserpine (Sigma-Aldrich, United States) was used as internal standard.

Fungal Plate Confrontation Assays
Trichoderma reesei strains were cultivated in MA medium containing 1% (w/v) D-glucose at 30 • C at 180 rpm for 48 h. The cultivation supernatant was filtered using a 2-µm-pore syringe filter, mixed 1:1 (v/v) with melted potato dextrose agar that contained additional agar-agar, and poured into sterile petri dishes. The resulting plates were inoculated with agar pieces overgrown with plant pathogenic fungi, which were pre-grown on potato dextrose agar, and were incubated at room temperature. For fungal confrontation assays, all strains were pre-grown on potato dextrose agar at room temperature. Overgrown agar pieces of a T. reesei strain and a plant pathogenic strain, respectively, were transferred to opposite sides of a single potato dextrose agar plate and the plates were incubated at room temperature.

Agar Diffusion Assay
Suspensions of the pathogenic bacterial strains were spread on PYE agar plates. Different filter disks each soaked with 30 µl of each supernatant obtained from 48 h of cultivation of T. reesei strains and, as control, unconditioned MA medium were placed circularly on each agar plate and incubated at 37 • C. The results were evaluated after 12, 36, and 50 h.

Construction of Recombinant T. reesei Strains to Characterize the Sorbicillin Cluster
In order to get insights into the biosynthesis of sorbicillinoids in T. reesei, we constructed a set of corresponding strains. We deleted the homolog of sorA, sor1 (Figure 2), which we expected to result in a complete abolishment of the biosynthesis pathway (Figure 1). We also deleted the homolog of sorC, sor3 (Figure 2), which we expected to interrupt the pathway prior to the oxidative dearomination of sorbicillin and 2 ,3 -dihydrosorbicillin (Figure 1). Further, we deleted sor4, and inserted ACRE_048140 (Figure 2); both genes were not characterized yet. All gene deletions were performed via homologous recombination using reestablishment of pyr4 as marker (Supplementary Figure S1A). The expression cassette for ACRE_048140 was inserted into the pyr4 locus as described earlier (Derntl et al., 2015;Supplementary Figure S2A). The genomic manipulations were confirmed by PCR analyses (Supplementary Figures S1B, S2B). Further, the absence of sor1, sor3, and sor4 transcripts in the corresponding deletions strains and the presence of ACRE_048140 transcripts in the strain A4814 were confirmed by RT-PCR. The ypr1 transcript was used as positive control for expression of the whole cluster (Supplementary Figures S1C, S2C).
Next, we cultivated the obtained strains sor1, sor3, sor4, and A4814 together with the strain QM6a, which contains the wild-type version of the gene cluster, the ypr1 deletion strain ypr1, and the ypr1 overexpression strain Reypr1 on glucose. As Ypr1 is the main regulator of the sorbicillin cluster, ypr1 is deficient in synthesis of sorbicillinoids, and Reypr1 produces high amounts of sorbicillinoids (Derntl et al., 2016). We monitored growth and biosynthesis of the yellow sorbicillinoids by measuring biomass accumulation and absorbance at 370 nm, respectively. As expected, the supernatants of ypr1, sor1, and sor3 did not turn yellow ( Figure 3A), but we could measure high absorbance for Reypr1 ( Figure 3A). A4814 had the same phenotype as QM6a, whereas sor4 secreted less yellow pigments than QM6a at time points after 36 h ( Figure 3A). Further, we observed that the strains ypr1 and sor1 produced more biomass than all the other strains ( Figure 3B). Vice versa, Reypr1 produced the lowest amount of biomass. The remaining strains grew equally well (Figure 3B). On a first glance, it appears as if the synthesis of the sorbicillinoids impairs growth of T. reesei.

Sorbicillinol Is the Main Product of the Biosynthesis Pathway
We were interested in what kind of sorbicillinoids were produced and accumulated in the recombinant strains. Consequently, we performed an LC-MS analysis of the culture supernatants from the above-described growth experiment on glucose (Figure 3). We used samples from early, middle, and late time points (24, 36, and 72 h). All compounds that were detected in the supernatant of any strain that produces sorbicillinoids (QM6a, Reypr1, sor4, or A4814), but not in strains that are deficient in sorbicillinoid synthesis ( ypr1 and sor1) are listed in Table 2. In the supernatant of strain sor3, in which biosynthesis is assumed to be interrupted after sorbicillin generation (Figure 1), only traces of sorbicillinol were detected ( Table 3). We consider them to be the result of chemical conversions and not of enzymatic activity. Further, the two compounds J_207 and F193 were also present in sor3 (Table 3). We suggest that J_207 and F193 might be degradation products of sorbicillin or precursors that occur during sorbicillin biosynthesis, because they have lower masses than sorbicillin (Table 2). Therefore, we did not include them in further data interpretations. However, we also detected a series of compounds containing up to nine nitrogen atoms in the sorbicillinoid producing strains ( Table 2). We consider these compounds (D_293, C_309, E_333, M_479, A_556, and S_657) to be the result of a sponanteous chemical reaction of sorbicillinoids with urea and/or ammonium ions in the medium. Therefore, we decided to omit also these compounds in further data interpretations.
Next, we grouped the remaining metabolites (given in bold letters in Table 2) into three categories, i.e., (i) metabolites that occur in elevated levels at early time points of cultivation, (ii) metabolites that occur in elevated levels only in sor4, and (iii) metabolites that occur in elevated levels at late time points of cultivation.
First, we had a detailed look on the presence and amounts of metabolites in QM6a, which is wild-type regarding the sorbicillinoids synthesis. The main early compound is sorbicillinol (Figure 4) or to be more precisely, sorbicillinols, because we detect two compounds with the same mass that have very similar retention times ( Table 2). We consider them to be identical. Further, we detect small amounts of sorbicillin in the beginning (Figure 4). These findings are in concordance with the previously suggested biosynthesis pathway in P. chrysogenum, which claims that sorbicillin is oxidized to sorbicillinol (Figure 1). Further, we also detected 5-hydroxyvertinolide and the compound K_307 in the early stages of cultivation (Figure 4). The strain A4814 produced nearly the same amounts of all the early compounds as QM6a (Figure 4). We detected higher amounts of all early metabolites in the ypr1 overexpression strain Reypr1 compared to QM6a (Figure 4). In sor4 we detected substantially lower amounts of sorbicillinols, 5-hydroxyvertinolide, and the compound K_307 than in QM6a, but slightly higher amounts of sorbicillin (Figure 4).

Deletion of sor4 Leads to Accumulation of Dihydrosorbicillinol
In the sor4 strain, we observed high amounts of two compounds that were annotated to dihydrosorbicillinol ( Table 2). The compound J_251 was predominantly detected at early time points, while F_251 accumulated throughout growth ( Figure 5). Notably, these compounds were not detected in any other strain. We also found higher amounts of F_265, which was annotated to oxosorbicillinol or epoxysorbicillinol (Table 2), in strain sor4 compared to the other strains after 24 and 36 h of cultivation ( Figure 5).

Sorbicillinol Is the Building Block for the Other Sorbicillinoids
Next, we analyzed the abundance and occurrence of the late sorbicillinoids (Figure 6). We detected disorbicillinol, bisvertinolon, and two compounds that were annotated to oxosorbicillinol or epoxysorbicillinol, and traces of a dihydrobisvertinolone in QM6a (Figure 6). In Reypr1, we measured higher levels of these late sorbicillinoids (Figure 6). Notably, Reypr1 also produces higher amounts of sorbicillinols at the early and middle time points (Figure 4). Vice versa, in A4814, lower amounts of sorbicillinols were detected than in QM6a after 36 h (Figure 4), and also lower amounts of the late sorbicillinoids (Figure 6). In sor4, which produces very small amounts of sorbicillinols (Figure 4), we detected none of the late sorbicillinoids (Figure 6). This suggests that the late sorbicillinoids are the products of chemical and/or enzymatical conversion of sorbicillinol. Interestingly, the late metabolite Q_499 is found in small amounts in all strains (Figure 6). Its empirical formula suggests that is an unknown bicyclic sorbicillinoid ( Table 2).

The Sorbicillinoids of T. reesei Restrict Growth of Pathogenic Fungi But Not of Bacteria
The cultivation experiment on glucose pointed toward a growth limiting effect of sorbicillinoids on T. reesei. Consequently, we were interested, whether these metabolites might also influence growth of plant pathogenic fungi. To test this, supernatants from 48 h of cultivation of the T. reesei strains QM6a, ypr1, Reypr1, and sor4 were filtered and added to cultivation medium in order to cast plates containing the sorbicillinoids. On these plates, the plant pathogenic fungi A. alternata, B. cinerea, F. oxysporum, and R. solani were inoculated. Here we observed a clear growth impairment of all four tested fungi caused by the secreted sorbicillinoids of T. reesei (Figure 7). On plates containing the supernatant of ypr1, the four fungi grew the same as on plates containing medium that was not mixed with any cultivation supernatant (control). All plant pathogenic fungi grew slower on plates containing sorbicillinol  and the derived sorbicillinoids (i.e., supernatant of cultivation of T. reesei strains QM6a and Reypr1). Notably, the growth inhibiting effect seems to be in direct relation to the amount of sorbicillinoids in the plate. We observed a less pronounced inhibition of growth of A. alternata, B. cinerea, and R. solani on plates with the supernatant of sor4, which contains dihydrosorbicillinols and only small amounts sorbicillinol and no complex sorbicillinoids (Figures 4-6). F. oxysporum was not affected by the sor4 supernatant (Figure 7). Interestingly, the mycelium of R. solani had a more compact and organized appearance on sor4 supernatant than on the other plates. The growth of A. alternata is only marginally inhibited on sor4 supernatant, but sporulation is augmented compared to the control plate and ypr1 (Figure 7). We were also interested, whether the sorbicillinoids might also have antibacterial activities. To this end, we used the supernatants of QM6a, ypr1, and Reypr1 cultivated for 48 h on glucose in an agar diffusion assay against strains of E. coli, A. baumanii, S. aureus, and MRSA. As control, we used medium, which had not been inoculated with any T. reesei strain. None of the culture supernatants had a growth inhibiting effect on any of the tested bacteria (not shown).

Sorbicillinoids Influence the Competition with Plant Pathogenic Fungi
Next, we aimed to test whether the growth limiting properties of the sorbicillinoids might support T. reesei in confrontation with other fungi. To this end, we performed confrontation plate assays using the plant pathogenic fungi A. alternata, B. cinerea, F. oxysporum, and R. solani and the following T. reesei strains: the wild-type QM6a, the sorbicillinoid nonproducer ypr1, the sorbicillinoid hyper-producer Reypr1, and sor4 which has a different sorbicillinoid spectrum [i.e., dihydrosorbicillinols, small amounts of sorbicillinol (Figures 4,  5), but no other sorbicillinoids (Figures 4, 6)]. Interestingly, T. reesei sor4 produced as much, or even more, yellow pigments as QM6a on the plates (Figure 8), in contrast to liquid cultures ( Figure 3A). We comment on this in the Section "Discussion." However, we observed no differences at all among the four T. reesei strains in confrontation to A. alternata (Figure 8). In confrontation with the other three fungi, we observed only subtle differences among the four T. reesei strains. Against B. cinerea, ypr1 grows weaker in vicinity to B. cinerea than the other strains at early time points. In confrontation with F. oxysporum, ypr1 gets stronger overgrown than QM6a, while Reypr1 is able to resist F. oxysporum better than the other strains. Notably, the FIGURE 4 | Abundance of compounds that are secreted early into the supernatant. The indicated T. reesei strains were cultivated on glucose in three replicates (compare Figure 3) for the indicated periods. A representative sample for each time point and strain was analyzed by HPLC-MS in technical duplicates. The quantities of the indicated compounds are given as response ratios (i.e., the fold-change of the compound detected in relation to the internal standard).
FIGURE 5 | Abundance of compounds that accumulate in the supernatant over time. The indicated T. reesei strains were cultivated on glucose in three replicates (compare Figure 3) for the indicated periods. A representative sample for each time point and strain was analyzed by LC-MS in technical duplicates. The quantities of the indicated compounds are given as response ratios (i.e., the fold-change of the compound detected in relation to the internal standard).
FIGURE 6 | Abundance of compounds that are elevated in the supernatant of strain sor4. The indicated T. reesei strains were cultivated on glucose in three replicates (compare Figure 3) for the indicated periods. A representative sample for each time point and strain was analyzed by LC-MS in technical duplicates. The quantities of the indicated compounds are given as response ratios (i.e., the fold-change of the compound detected in relation to the internal standard). mycelium of F. oxysporum overgrowing sor4 has a more structured and denser morphology than on the other T. reesei strains. Surprisingly, the sorbicillinoid non-producer ypr1 most strongly overgrows R. solani in comparison to the other T. reesei strains (Figure 8).

DISCUSSION
On the Biosynthesis Pathway of Sorbicillinoids, 5-Hydroxyvertinolide, and the Roles of Sor4 and ACRE_048140 Previously, P. chrysogenum SorC was demonstrated to oxidize sorbicillin and 2 ,3 -dihydrosorbicillin to sorbicillinol and 2 ,3 -dihydrosorbicillinol, respectively, in vitro (Fahad et al., 2014). Based on these results, in silico analyses of the PKS SorA and SorB, and previous data from metabolite identifications and radio-labeled feed experiments, the authors proposed the model described in Figure 1. An earlier model had claimed that oxosorbicillinol was a key intermediate for sorbicillinol synthesis via a hypothetical compound, and that sorbicillin was derived from sorbicillinol . Our data support the newer model proposed by Fahad et al. (2014), because we detected the highest amounts of sorbicillin at the earliest time point, and because we observed the accumulation of oxosorbicillinol only after sorbicillinol had been built, timely and hierarchically (Figures 4, 6).
The model of  also suggested that 5-hydroxyvertinolide was derived from the same hypothetical compound as sorbicillinol. Another, previous model proposed that epoxysorbicillinol could be converted into 5-hydroxyvertinolide (Sperry et al., 1998). Our observations do not support any of these two models, because we detect 5-hydroxyvertinolide already at early time points in dependence of sorbicillinol (Figure 4). We consider 5-hydroxyvertinolide, as well as the unknown, early metabolite K_307 (Table 2), to be (chemically and/or enzymatically) derived from sorbicillinol because they are barely present in sor4 (Figure 4).
FIGURE 7 | Influence of T. reesei sorbicillinoids on growth of plant pathogenic fungi. The T. reesei strains (indicated on top) were cultivated on glucose for 48 h and the resulting culture supernatants were filtered, mixed 1:1 (v/v) with potato dextrose agar, and poured into petri dishes. As a control, medium that was not inoculated with any T. reesei strain was used. The plant pathogenic fungi (indicated on the left) were inoculated on these plates and incubated at room temperature. Pictures were taken after 3 days in the case of R. solani and B. cinerea, and after 5 days in the case of F. oxysporum and A. alternata. The diameters of the growth halos are provided.
However, we would like to extend the model proposed by Fahad et al. (2014) on the biosynthesis of sorbicillinol in T. reesei. We observed the accumulation of the two compounds, J_251 and F_251, that were both annotated to dihydrosorbicillinol ( Table 2), but only small amounts of sorbicillinol in T. reesei sor4 (Figures 4, 5). Notably, we did not detect the dihydrosorbicillinols in any other strain. We speculate, that the early arising compound J_251 might be 2 ,3dihydrosorbicillinol. This assumption is based on the already existing model, which proposes that 2 ,3 -dihydrosorbicillinol is synthesized in parallel to sorbicillinol (Fahad et al., 2014) (Figure 1). Sor4 is an FAD-binding dehydrogenase according to a conserved domain analysis. These enzymes are able to reduce alkanes to alkenes, because FAD has such a high reduction potential that it can accept two electrons and two protons simultaneously. The accumulation of dihydrosorbicillinol in the absence of Sor4 suggests that Sor4 might reduce the doublebond in the linear side-chain of 2 ,3 -dihydrosorbicillinol (4). This implies that the main product of the PKS-cascade and the cyclization reaction is in fact 2 ,3 -dihydrosorbicillin (2), which is oxidized to 2 ,3 -dihydrosorbicillinol (4) by Sor3. Finally, Sor4 might reduce 2 ,3 -dihydrosorbicillinol (4) to sorbicillinol (3).
Alternatively, Sor4 might as well reduce 2 ,3 -dihydrosorbicillin (2) to sorbicillin (1) before it is oxidized to sorbicillinol (3) by Sor3 in a final step ( Figure 9A). Of course, the two alternative pathways might occur simultaneously. F_251, the second dihydrosorbicillinol in the sor4 supernatant (Figure 5), could be 2 ,5 -dihydrosorbicillinol or 4 ,5 -dihydrosorbicillinol or another unknown isomer; 2 ,5 -dihydrosorbicillin was previously identified as a product of chemical hydrogenation of sorbicillin (Trifonov et al., 1983). If F_251 was 2 ,5 -dihydrosorbicillinol, its occurrence could be explained by a chemical conversion. This speculation is supported by the fact that F_251 accumulates over time ( Figure 5). Alternatively, it could be another unknown isomer that occurs by chemical isomerization. If F_251 was 4 ,5dihydrosorbicillinol, its occurrence could be explained by the implied sloppy mode of action of SorA/1, as already discussed by Fahad et al. (2014). The PKS SorA/1 contains, next to the core acyl transferase subunit, a presumably non-functional methyl transferase subunit, a ketoacyl reductase subunit, and an enoyl reductase subunit. The ketoacyl reductase subunit reduces the beta-carbonyl of the growing polyketide to a hydroxyl group. The enoyl reductase subunit reduces a double to single FIGURE 8 | Influence of T. reesei sorbicillinoids on direct fungal confrontations. The T. reesei strains (indicated on top) and plant pathogenic fungi (indicated on the left) were inoculated on potato dextrose agar plates at opposite sides (T. reesei on the right, the plant pathogenic fungi on the left) using equal pieces of overgrown potato dextrose agar, and incubated at room temperature. Pictures were taken after 2 days after initial contact in the case of B. cinerea, and after 3 weeks in the case of F. oxysporum, R. solani, and A. alternata.
Further, if we speculate that the ketoacyl reductase subunit of Sor1 was not active during a cycle of polyketide elongation, a sorbicillinol derivate containing a keto-group would be synthesized ultimately (Figure 9C, compound 6). This hypothetical compound has the same empiric formula as oxosorbicillinol and epoxysorbicillinol, and might be compound F_265 ( Table 2) that was detected in all sorbicillinoid producing strains (Figure 5). Its higher levels in sor4 could be explained by a feedback of the accumulating dihydrosorbicillinol on Sor1.
The amounts of early metabolites were nearly equal in the wild-type-like QM6a and the strain A4814, which bears the hydrolase ACRE_048140 from A. chrysogenum (Figure 4). We detected smaller amounts of the late metabolites in A4814 than in QM6a (Figure 6), but we consider this to be the result of lower sorbicillinol levels in strain A4814 (Figure 4) because these metabolites are derived from sorbicillinol. Therefore, we conclude that the hydrolase ACRE_048140 did not influence the sorbicillinoid biosynthesis in T. reesei.

On the Growth-Limiting Properties of Sorbicillinoids
During the growth experiment of the recombinant strains, we observed that the growth rate seems to be negatively related to the biosynthesis of sorbicillinoids (Figure 3). The strains, which do not produce sorbicillinoids, ypr1 and sor1, grew better than the wild-type-like strains QM6a and A4814, whereas the sorbicillinoid hyper-producer Reypr1 accumulated less biomass. Interestingly, sor3 and sor4 grew equally well as the wild-type-like strains, although they do not produce mature sorbicillinoids. In sor3 the biosynthesis  Figure 1). Briefly, the PKS Sor1 iteratively combines three acetyl units and the growing chain is modified by the ketoacyl reductase subunit, and optional by the enoyl reductase subunit in the second cycle. The polyketide is handed over to the PKS Sor2, which adds three more acetyl units, and two methyl groups. Sor2 releases an aldehyde, which undergoes spontaneous cyclization resulting in the formation of sorbicillin (1) or 2 ,3 -dihydrosorbicillin (2). Next, the FAD-dependent monooxygenase Sor3 transforms sorbicillin or 2 ,3 -dihydrosorbicillin to sorbicillinol (3) or 2 ,3 -dihydrosorbicillinol (4). Further, Sor4 might reduce 2 ,3 -dihydrosorbicillin (2) to sorbicillin (1) and/or 2 ,3 -dihydrosorbicillinol (4) to sorbicillinol (3). The obtained data from our study (Figures 4-6) suggest that the main product of Sor1/2 is in fact 2 ,3 -dihydrosorbicillin. (B) Assumed that the enoyl reductase subunit of Sor1 was active during the first cycle, 4 ,5 -dihydrosorbicillinol (5) would have been synthesized by Sor2 and Sor3. (C) Assumed that the ketoacyl reductase subunit of Sor1 was not active during the third cycle, Sor2 and Sor3 would have assembled 3 -keto-sorbicillinol (6).
pathway is disrupted after the cyclization of the released aldehydes (Figure 1), implying that intracellular sorbicillin and dihydrosorbicillin are built. In sor4 dihydrosorbicillinol accumulates, very small amounts of sorbicillinol are synthesized, and no late sorbicillinoids are produced (Figure 6). Therefore, the reason for the growth limitation is likely to be either the metabolic burden or the intracellular presence of sorbicillin and dihydrosorbicillin (Figure 9). It cannot be excluded that the extracellular sorbicillinoids also have a growth limiting effect on T. reesei. Probably, all three factors contribute to some extents to the observed growth restrictions.
During the confrontation assays on the plates, we observed that sor4 turned the medium more intensely yellow than the wild-type-like QM6a (Figure 8), but it was the opposite in liquid cultures ( Figure 3A). Generally, we observed that the medium in plates became more yellow than in liquid cultures. We speculate that oxygen and/or light might degrade the sorbicillinoids. The strain sor4 produces predominantly dihydrosorbicillinols (Figure 5), which might be more susceptible to degradation by light and/or oxygen than sorbicillinol and the late sorbicillinoids. The presence of the dihydrosorbicillinols and the lack of the mature sorbicillinoids explain why sor4 did influence neither the morphology of the other fungi nor their growth rates.
The extracellular sorbicillinol and/or mature sorbicillinoids had a clear influence on the growth of other fungi (Figure 6). In direct fungal confrontations, the sorbicillinoids supported growth of T. reesei in presence of B. cinerea and F. oxysporum, however, subtle the effects were. In confrontation against B. cinerea, the radical-scavenging properties and/or the chemical reactivity of the sorbicillinoids might protect T. reesei from secreted compounds and/or proteins of B. cinerea. In confrontation against F. oxysporum and R. solani, the growthinhibiting effects pose an obstacle for the stronger fungus. This is, the sorbicillinoids delayed F. oxysporum in overgrowing T. reesei, but they also hindered T. reesei from overgrowing R. solani. Taken together, the sorbicillinoids pose a possibility for T. reesei to maintain its already claimed territory in confrontation with other fungi, but it has to put up with their growth limiting effects.

AUTHOR CONTRIBUTIONS
CD constructed the plasmids, the deletion strains, performed the growth experiment, and the fungal confrontation assays, performed data analysis, co-drafted the manuscript, and was involved in the study design. FG-C performed the LC-MS analyses and performed data analysis. TM-d-S constructed the strain A4814. H-JB performed the agar diffusion assay. AD performed data analysis. RM was involved in the study design. AM-A co-drafted the manuscript and was involved in the study design.

FUNDING
This work was supported by two grants from the Austrian Science Fund (FWF): [P26733, P29556] given to AM-A and RM, respectively.