Infection with Classical Swine Fever Virus Induces Expression of Type III Interferons and Activates Innate Immune Signaling

Classical swine fever virus (CSFV) commonly infects the lymphatic tissues and immune cells of pigs and could cause a lethal disease in the animals. The process and release of cytokines like type III interferons (IFNs) is one of the important responses of the host innate immunity to viral infection. However, little information is available about type III IFN response to the CSFV infection. In this study, we investigated the expression of type III IFNs including interleukin-28B (IL-28B) and IL-29 in PK-15 cells and pigs following CSFV infection. We found that infection with CSFV was able to induce expression of IL-28B and IL-29 in PK-15 cells, although the increased levels of type III IFNs were limited. Importantly, up-regulation of IL-28B and IL-29 was further observed in CSFV infected animal tissues. The production of IL-28B and IL-29 was reduced by the inactivation of NF-κB in cells, indicating that activated NF-κB is required for efficient expression of type III IFNs induced by CSFV. Moreover, our experiments demonstrated that infection with CSFV strongly stimulated the downstream of STAT1 signaling in vitro and in vivo. In addition, several critical IFN-stimulated genes (ISGs) including IFITM3, OASL, OAS1, and ISG15 were significantly upregulated at both mRNA and protein levels in PK-15 cells and infected pigs. Together, these results reveal that CSFV can trigger host antiviral immune responses including production of type III IFNs, activation of STAT1, and induction of some critical ISGs.


INTRODUCTION
Host innate immunity against viruses is stimulated by sensing the viral infection via pattern recognition receptors (PRRs). Such receptors include Toll-like receptors (TLRs), NOD like receptors and the cytosolic retinoic acid-inducible gene I (RIG-I) and melanoma differentiationassociated protein 5 (MDA-5), which recognize different conserved microbial structures and molecules, such as single-stranded and double-stranded viral RNA, collectively termed as pathogen associated molecular patterns (PAMPs). The interaction between host PRRs and viral PAMPs is a critical step for initiation of innate immune response. Upon detecting PAMPs, PRRs activate the intracellular signaling pathways that regulate both immediate innate and subsequent adaptive immunity (Iwasaki and Medzhitov, 2010;Goraya et al., 2015). A vital role of innate antiviral response is the rapid induction of interferons (IFNs), which further stimulates a cascade of IFN-stimulated genes (ISGs) whose expression shapes antiviral and immune-modulatory actions that help to limit the viral infection and replication (Takaoka and Yanai, 2006). It is known that expression of type I and type III IFNs depends upon the activation of interferon regulatory factor-3 (IRF-3), IRF-7 and nuclear factor-κB (NF-κB) via TLRs and RIG-I dependent signaling pathways (Janeway and Medzhitov, 2002). These transcription factors are also involved in regulating production of other cytokines and chemokines. Type III IFNs consist of three members in human, named as interleukin-29 (IL-29), IL-28A, and IL-28B, also known as IFN-λ1, IFN-λ2, and IFN-λ3, respectively. Type I and type III IFNs are considered to be the most important antiviral molecules of innate immune response against viral infection (Borden et al., 2007;Wang et al., 2014;Wei et al., 2014). However, little is known about how type III IFNs respond to classical swine fever virus (CSFV) infection.
Classical swine fever virus (previously called hog cholera virus) belongs to the genus Pestivirus of Flaviviridae family. CSFV is a spherical viral particle, about 40-60 nm in diameter, has positive single-strand RNA (ssRNA) genome of 12.3 kb surrounded by lipid bilayer (Meyers et al., 1989). The ssRNA of the virus carries a large open reading frame (ORF) and encodes a single polyprotein that is co-and post-translationally cleaved to produce 12 proteins by cellular and viral proteases: N-terminal auto-protease (N pro ), C, E rns , E1, E2, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B (Meyers and Thiel, 1996). CSFV is a highly infectious virus responsible for a contagious infection known as classical swine fever (CSF) in pigs. Clinical symptoms and severity of CSF vary according to different strains of the virus, age of the host and the immune status of the herd (Terpstra, 1987;Moennig et al., 2003). After establishing an infection in the tonsils, CSFV invades into host lymphatic organs through blood, with lethal outcome including death of piglets within the infected populations (Susa et al., 1992;Rossi et al., 2011). CSFV is commonly found in T cells, B cells and monocytes (Qu et al., 2001). Persistence of CSFV in lymphoid tissues is considered to be the most significant feature of CSFV infection, which leads to the acquired immunosuppression condition and is associated with pathogenesis of the virus (Summerfield et al., 1998;Ambagala et al., 2005). In addition, it is thought that virulence of CSFV is associated with mutations in several viral proteins including N pro , C, E rns , E1, E2, p7, and NS4B, which are related to viral invasion, uncoating, replication and interaction with cellular factors (Leifer et al., 2013;Ji et al., 2015).
Two crucial PAMPs can be sensed by cellular receptors during CSFV infection: one is the viral genomic ssRNA and the second is double-strand RNA (dsRNA), an intermediate replication product of the virus. The host TLR-3, 7, and 8 are responsible for sensing the RNA viruses (Kawai and Akira, 2006). TLR-7 binds with the ssRNA derived from CSFV and TLR-3 senses the replication intermediate dsRNAs (Diebold et al., 2004;Sen and Sarkar, 2005;Chen et al., 2015). Intracellular detection of ssRNA by TLR-7 triggers the activation of transcription factors IRF-7 and NF-κB, which ultimately initiate the expression of type I IFNs including IFNα and IFNβ (Lund et al., 2004;Cao et al., 2015). TLR-3 signaling activates the transcription factor IRF-3, which induces expression of the IFN-β and type III IFNs upon its entrance into the nucleus of cells (Kawai and Akira, 2006;Chen et al., 2015). It has been established that the presence of viral RNA in the cytoplasm can be detected by cellular RIG-I and MDA-5 (Yoneyama et al., 2005). The interaction between viral dsRNA and RIG-I or MDA-5 causes robust expression of IRF-3 dependent IFNs . In turn, IFNs can stimulate the gene expression of IRF-7, which results in an enhanced expression of type I IFNs and induces the transcription of antiviral proteins, such as ISG15, interferoninduced transmembrane proteins (IFITMs) and Mx A (Ouyang et al., 2014).
Although progress has been made in understanding host immune response to CSFV infection and immunosuppression caused by the virus, activation of innate immune signaling during CSFV infection and mechanisms underlying the innate immunity against CSFV are not fully understood. In particular, it is still unclear whether type III IFNs is induced by CSFV infection, and little is known about activation of STAT1 and how some critical ISGs are regulated during CSFV infection in vitro and in vivo. In this study, we analyzed the expression of type III IFNs and some critical ISGs in PK-15 cells infected with CSFV. Furthermore, these important molecules involved in host innate immunity were examined in pigs after CSFV infection. We observed that mRNA levels for IL-28B and Il-29 were increased both in vitro and in vivo after the infection with CSFV. Furthermore, CSFV infection greatly activated STAT1 and increased the expression of different ISGs, including IFITM3, ISG15, OAS1, and OASL. Interestingly, inactivation of NF-κB significantly reduced the expression of IL-28B and IL-29. These results demonstrate that CSFV can induce host innate antiviral response involving the expression of Type III IFNs and some critical ISGs.

Virus and Cells
Classical swine fever virus Shimen strain was obtained from Prof. Jinding Chen (The South China Agriculture University, Guangzhou, China). To determine the virus titers, swine kidney PK-15 cells were cultured in 96 well plates and inoculated with 10 fold serial dilutions of CSFV Shimen strain. After infection, cells were incubated at 37 • C for 5 days. Then, PK-15 cells were fixed with 80% acetone at −20 • C for 30 min, and viruses were detected by an immunofluorescence assay using mouse anti-CSFV E2 antibody and FITC-conjugated goat anti-mouse secondary antibody. Virus titers were calculated according to Kaerber Knoetig (Knoetig et al., 1999) and expressed as 50% tissue culture infectious doses (TCID 50 ) per milliliter. The multiplicity of infection (MOI) was calculated based on TCID 50 . 293T cells and swine kidney cell line PK-15 were purchased from American Type Culture Collection (Manassas, VA, United States). 293T cells and PK-15 cells were maintained in complete Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics. All cells were incubated at 37 • C with 5% CO 2 .

Viral Infection
PK-15 cells were grown to approximately 80% confluence in cell culture plates and then infected with CSFV at an MOI of 1∼3. The mock cells were treated with the blood of healthy pigs. Then cells were placed at 37 • C with 5% CO 2 for 1 h. Later, cells were washed three times with PBS and cultured in DMEM supplemented with 2% FBS at 37 • C with 5% CO 2 . Cultured cells were harvested at different time points. Total RNA and proteins were isolated from the cells and further used for qRT-PCR, PCR and Western blotting. For in vivo experiments, five pigs (40 days old) were purchased from a farm, which was CSFVnegative. All pigs were infected by intramuscularly injecting 0.1 ml (TCID50 = 10 −4.25 /0.1 ml) of CSFV Shimen strain or mock per pig. All pigs were reared under the same conditions. At 5 days post-infection, lymphoid tissues (spleen, tonsils, inguinal lymph node, sub-maxillary lymph node, and mesenteric lymph node) of infected or control animals were collected for further studies.

RT-PCR and Quantitative Real-time PCR
Total RNA was isolated from cultured cells and lymphoid tissues of pigs using TRIzol reagent (Invitrogen, Carlsbad, CA, United States) and reverse transcribed into cDNA utilizing M-MLV Reverse Transcriptase (Promega, United States) according to the manufacturer's instructions. The cDNA was analyzed by qRT-PCR using TransStart Green qPCR SuperMix (TransGen Biotech) and PCR using rTaq DNA polymerase (Takara Bio). The amplified products by PCR were resolved on 1% agarose gels, and when necessary, the intensity of bands was analyzed using Quantity One software (Bio-Rad, United States), as previously described . The primers specific for CSFV E2, pig IL-28B, IL-29, OAS1, OASL, IFITM3, ISG15, and β-actin (the reference housekeeping gene for internal standardization) were designed using the Primer 5 software ( Table 1). The data of qRT-PCR analysis were shown in normalized ratios which was auto-calculated using C T method by LightCycler system (Roche, Switzerland).

Enzyme-Linked Immunosorbent Assay (ELISA)
Cell culture supernatants were collected from the culture medium of PK-15 cells infected with CSFV Shimen strain, as a source of virus-induced cytokines. To quantify production of IL-28B and IL-29 by host cells, cell culture supernatants from virus infected

Primer name
Primer sequence (5 -3 ) cells was harvested and examined by ELISA using the ready SET-Go of pig IL-28B or IL-29 analysis kit (eBioscience, San Diego, CA, United States) according to manufacturer's instruction.

Statistical Analysis
Results were shown as mean values ± standard error (mean ± SE). Statistical significance was determined by Student's t-test analysis. A level of P < 0.05 was considered to be significant.

Ethics Statement
The animal protocols used in this study were approved by the Research Ethics Committee of College of Animal Science, Fujian Agriculture and Forestry University (Permit Number PZCASFAFU2014003). The procedures were carried out in accordance with the approved guidelines and the regulations for the Administration of Affairs Concerning Experimental Animals approved by the State Council of China.

Expression of Type III IFNs Is Upregulated in PK-15 Cells during CSFV Infection
To better understand host innate immune response to CSFV infection, we investigated the cellular transcriptional response of type III IFNs, important components of innate immunity, during CSFV infection of PK-15 swine kidney cells. For this, PK-15 cells were infected with CSFV Shimen strain, and the samples were harvested at different time points of postinfection and examined for expression of CSFV gene and type III IFNs. CSFV was well-replicated in the cells as indicated by robust expression of CSFV E2 gene that encodes one of CSFV structural protein (Figures 1A,B and Supplementary Figure S1). Importantly, we observed that expression of swine IL-28B and IL-29, two members of type III IFN family in pig, was clearly upregulated during CSFV infection (Figures 1A,B).
The highest mRNA levels of IL-28B and IL-29 were found at 36-48 h post-infection with CSFV. This finding was further confirmed by independent analysis using quantitative real-time PCR (Figures 1C,D), although the increased levels of type III IFNs were limited.

CSFV Infection Causes Increased Expression of Type III IFNs in Vivo
Next, we determined whether CSFV infection could activate type III IFN response in vivo. Pigs were infected with CSFV (0.1 ml per pig, TCID50 = 10 −4 /0.1 ml) and spleen, heart, liver, lungs, kidney, mesenteric lymph node, inguinal lymph node, sub-maxillary lymph node, tonsils, and serum of infected animals were collected 5 days post-infection. We found that high levels of CSFV E2 gene expression were detected in the spleen, mesenteric lymph node, inguinal lymph node, sub-maxillary node, and tonsils after challenge with the virus (Supplementary Figure S2A). Thus, these lymphoid organs were selected for further studies. The expression of swine IL-28B and IL-29 were then examined by RT-PCR ( Supplementary Figures S2B-E) and quantitative real-time PCR (Figures 2A-D). Using RT-PCR, we observed that expression of IL-28B and IL-29 was clearly induced in spleen, inguinal lymph node, mesenteric lymph node and tonsils of CSFV infected pigs, whereas their expression levels were very low in these organs from mock-treated control animals ( Supplementary Figures S2B-E). Similarly, results from analysis using quantitative real-time PCR showed that expression of IL-28B and IL-29 was significantly increased in spleen, mesenteric lymph node, inguinal lymph node, and sub-maxillary node in pigs infected with CSFV as compared to that in these organs of control animals (Figures 2A-D). In particular, mRNA levels of IL-29 in spleen, mesenteric lymph node, and sub-maxillary node of the pigs were the most highly elevated after the virus infection (Figures 2A,B,D). Furthermore, protein levels of IL-28B and IL-29 were examined in pig serum by ELISA. The data exhibited that IL-29 protein levels were markedly increased in serum of infected pigs as compared with the control animals, but no significant change in IL-28B protein levels was detected in pig serum after infection with the virus (Figures 2E,F). Taken together, these experiments demonstrate that infection with virulent CSFV Shimen strain can induce the expression of type III IFNs both in vitro and in vivo.

NF-κB Is Involved in Regulating the Expression of Type III IFNs Induced by CSFV
NF-κB, an inducible transcription factor, plays a variety of evolutionarily conserved roles in the immune system (Hayden and Ghosh, 2014). In an attempt to provide an insight into the mechanism underlying the expression of type III IFNs during CSFV infection, we tested whether there was a functional link between induced expression of type III IFNs and activation of NF-κB, a key transcriptional factor downstream of the interaction between host PRRs and viral PAMPs (Ank et al., 2006b). To this end, cells were infected with CSFV and IκB-α protein was examined. We found that the protein level of IκB-α, an inhibitor of NF-κB, decreased in PK-15 cells at different time points (12-36 h) post-infection ( Figure 3A). Therefore, we hypothesized that NF-κB might be a key factor in regulating the expression of type III IFNs induced by CSFV. To test this possibility, PK-15 cells were treated with either BAY11-7082 (a selective inhibitor of NF-κB) or DMSO (control) and then infected with CSFV. Indeed, the CSFV-induced expression of IL-28B and IL-29 was reduced in NF-κB inhibited cells ( Figure 3B). These data suggest that NF-κB may play an important role in regulating the production of IL-28B and IL-29 during the CSFV infection.

CSFV Infection Activates STAT1 in Vitro and in Vivo
In natural host, the strength and the duration of cytokine expression and cytokine-activated signaling are tightly regulated (Cao, 2016). Since type III IFNs were induced during the CSFV infection, we asked whether their downstream signaling was normally activated. Production of type III IFNs is regulated through similar signal transduction pathways as type I IFNs (Ank et al., 2006b;Zhang et al., 2008), and these IFNs primarily activate the JAK-STAT1 signal pathway to achieve their antiviral function (Egli et al., 2014). Therefore, to address this question, we sought to investigate activation of STAT1 during the viral infection. For this, a time course experiment was conducted in vitro. We found that phosphorylation of STAT1 in CSFV infected PK-15 cells was up-regulated at early stages of infection (24, 36, and 48 h p.i.), but slightly declined at later stages of infection (after 60 h p.i.) ( Figure 4A). Furthermore, phosphorylation status of STAT1 during the CSFV infection was examined in vivo. Strikingly, phosphorylation levels of STAT1 were greatly elevated in spleen of pigs infected with CSFV as compared to those in control animals ( Figure 4B). Similar results were also obtained from experiments examining STAT1 phosphorylation in inguinal lymph node of CSFV infected pigs ( Figure 4C). In addition, we observed that phosphorylation of STAT1 was also increased in sub-maxillary node and mesenteric lymph node in CSFV infected pigs, compared to that in control pigs (Figures 4D,E). These data suggest that effective activation of STAT1 signaling can be triggered by the CSFV infection.

Expression of Some Critical ISGs Is Induced by CSFV Infection in Vitro and in Vivo
As we found that CSFV infection induced the expression of type III IFNs and activation of STAT1, next we further tested whether CSFV infection could enhance the expression of some key ISGs in vitro and in vivo. For this, RT-PCR and quantitative real-time PCR was performed. As shown in Figure 5A, the mRNA expression of some key ISGs, including IFITM3, OAS1 and OASL, was induced in PK-15 cells during the CSFV infection. Expression of ISG15 was slightly increased at early stages during the CSFV infection, but the virus infection had no significant effect on expression of Mx1 ( Figure 5A). The increased mRNA expression of IFITM3, OAS1, OASL, and ISG15 in infected PK-15 cells was further confirmed by quantitative real-time PCR (Figure 5B and Supplementary Figure S3). mRNA expression of these ISGs was then examined in vivo. Using RT-PCR, we observed that expression of IFITM3, ISG15, OAS1, and OASL was markedly up-regulated in spleen, mesenteric lymph node, inguinal lymph node, sub-maxillary node, and tonsils in pigs infected with CSFV as compared to that in the control animals ( Figures 5C,D and Supplementary Figure S4). Similarly, analysis by quantitative real-time PCR showed that expression of IFITM3, OAS1, and OASL was significantly increased in spleen, mesenteric lymph node, inguinal lymph node, and submaxillary node of infected pigs as compared with the controls (Figures 5E-H).
Moreover, protein expression of IFITM3 and ISG15 in pigs infected with CSFV was examined by Western blot analysis (Figures 6A-D). Indeed, we observed that IFITM3 and ISG15 protein levels were greatly elevated in spleen, mesenteric   PK-15 cells were infected with CSFV for indicated time, followed by analysis of Western blotting using indicated antibodies. (B-E) Pigs were infected with CSFV for 5 days as described in Figure 2, followed by Western blotting using indicated antibodies. Expression of STAT1 and its phosphorylation status were examined in (B) spleen, (C) inguinal lymph node, (D) sub-maxillary node, and (E) mesenteric lymph node. Shown are representative of three independent experiments. lymph node, inguinal lymph node, and sub-maxillary node in pigs after the infection (Figures 6A-D). Together, these results suggest that CSFV infection can trigger the innate immune response in pigs, which may play an important role in host defense against the virus infection in the early days.

DISCUSSION
Classical swine fever causes huge economic losses in pig industry particularly in developing countries (Li et al., 2013). Understanding pathogenesis and host antiviral response is critical for finding a feasible control strategy against CSFV infection. However, so far, the pathogenesis of CSFV and the host antiviral immune response are still not fully determined.
In the present study, we investigated the involvement of host innate immunity by assessing the expression of type III IFNs and activation of their downstream signaling, as well as expression of several critical ISGs after infection of PK-15 cells and pigs with CSFV Shimen strain. We observed that CSFV infection induced the expression of type III IFNs, enhanced the phosphorylation of STAT1, and caused up-regulation of several critical ISGs in PK-15 cells and pigs after the infection. The kinetics of type III IFNs, the ISGs and STAT1 activation in lymphoid tissues of pigs after the exposure to CSFV were similar to those in PK-15 cells, but their quantity appeared to be higher in the infected animals. These data indicate that CSFV can effectively activate the host innate immune system and thereby initiates a positive antiviral immune response both in vitro and in vivo.
It has been shown that infection with several single-stranded RNA viruses can induce the expression of type III IFNs (Brand et al., 2005). However, Type III IFNs can only be produced by specific cell types such as lymphoid cells, in contrast to type I IFNs which can be expressed by almost all cell types (Ank et al., 2006a). The interferon signaling pathways and their regulation are very complex and diverse. Here, we found that CSFV infection induced the expression of Type III IFNs, but the increased levels of type III IFNs caused by the virus were limited as compared to their expression levels induced by influenza A virus in our previous studies . These observations suggest that CSFV may have evolved a strategy to suppress the expression of type III IFNs. It is known that type III IFNs are induced through similar signal transduction pathways as type I IFNs (Ank et al., 2006b;Zhang et al., 2008), and both type I and type III IFNs activate the JAK-STAT signal pathway to achieve their antiviral function (Egli et al., 2014). For example, activation of signaling pathways mediated by TLR-3 and RIG-I, which have been well-characterized to induce IFN-α/β production, also induces type III IFNs in murine and human cells (Dumoutier et al., 2004;Onoguchi et al., 2007;Zhou et al., 2009). But unlike type I IFN receptor, type III IFN receptor is expressed in a cell-specific fashion (Sommereyns et al., 2008). It still remains to be determined how expression of type III IFNs is regulated and how they activate STAT1 in a cell-specific fashion during the CSFV infection.
Many viruses have potential to trigger the production of interferons to a certain extent. Previous studies have shown that a number of RNA viruses are able to trigger the expression of Type III IFNs (Coccia et al., 2004;Ank et al., 2006a). To establish a successful infection, however, RNA viruses can interrupt signaling pathways and perturb host antiviral defenses to evade the innate immunity. Limited induction of type III IFNs indicates that there exists a mechanism by which CSFV inhibits the signaling pathway regulating expression of type III IFNs. Therefore, it is of interest to study the mechanism underlying control of type III IFNs by CSFV. On the other hand, to combat the infection, host immune system deploys various antiviral cytokines that can activate a number of key downstream signaling pathways like JAK/STAT, which increases the production of effective antiviral molecules, including numerous ISGs (Takeuchi and Akira, 2009). In this study, we found that STAT1 was clearly activated in PK-15 cells following the CSFV infection. Furthermore, we observed that STAT1 was greatly activated in pigs infected with CSFV. It was previously revealed that CSFV infection caused an increased production of type I IFNs . Together, these results suggest that both type I and type III IFNs likely contribute to activation of STAT1 during the CSFV infection.
Further analysis determined that NF-κB is involved in regulation of the CSFV-induced expression of type III IFNs. It is well-known that after recognizing the viral RNA, RIG-I and MDA-5 interact with CARD and IPS-1 to recruit TRAF6, TBK1 and IKK, which activate the downstream signaling of transcription factors IRFs and NF-κB (Han et al., 2014;Goraya et al., 2015). A previous study revealed that CSFV was recognized by RIG-I and MDA-5 and activated the NF-κB signaling pathway, ultimately resulting in production of type I IFNs . NF-κB acts as a transcription factor that responds to cellular environment upon viral infection and controls the expression of innate molecules (Bonizzi and Karin, 2004). IκBα is a specific inhibitor of NF-κB, which interacts with NF-κB dimers and retains NF-κB in the cytoplasm of different cell types (Karin and Ben-Neriah, 2000). Degradation of the IκBα subunit leads to the release and translocation of NF-κB to the nucleus, which activates the transcription of IFN genes involved in innate immunity (Kanarek et al., 2010). In the present study, we found that the levels of IκBα decreased in response to CSFV infection, suggesting an increase of NF-κB activity. In order to elucidate the function of NF-κB in response to CSFV infection, cells were treated with BAY11, a NF-κB inhibitor, prior to CSFV infection. Interestingly, our experiments demonstrated that expression of IL-28B and IL-29 was significantly reduced in CSFV-infected cells after treatment with BAY11. Together, these findings suggest that NF-κB may play an important role in antiviral innate immunity against CSFV infection.
Type III IFNs functionally resemble type I IFNs, inducing antiviral protection in vitro (Kotenko et al., 2003;Robek et al., 2005) as well as in vivo (Ank et al., 2006b). Interaction between type III IFNs and their receptor enhances the phosphorylation of STAT family and the formation of the interferon-stimulated gene factor 3 (ISGF3) complex (Kotenko et al., 2003), and the induction of typical IFN-induced genes like the IFITM3, OAS1, OASL, and Mx1 genes (Sadler and Williams, 2008;Li et al., 2016). In the present study, we noticed that expression of several critical ISGs including IFITM3, OASL, OAS1, and ISG15 was upregulated in both PK-15 cells and lymphoid tissues of pigs infected with CSFV. A previous study revealed that OASL suppressed the CSFV replication via interacting with MDA-5, a double stranded RNA sensor, and enhanced type I IFN signaling (Li et al., 2017). However, precise roles of other ISGs in the innate immunity against CSFV are needed to be elucidated.
We found that expression levels of OAS1, IFTIM3, and OASL in CSFV infected pigs and control animals showed some variation among spleen, mesenteric lymph node, submaxillary lymph node and inguinal lymph node. It is likely that this is due to varied cytotropism of CSFV in pigs. Although we observed basal expression of IFITM3 and ISG15 in mesenteric lymph node and inguinal lymph node of mock infected animals, significant increase in the expression of these ISGs was seen in these lymphoid tissues of CSFV infected pigs as compared to that in the control animals. Interestingly, there was an unexpected expression of IFITM3 at a low level observed in a negative control pig. These data suggest that there is a possibility that expression levels of particular ISGs might be varied due to individual differences. Therefore, a large number of the animals might be needed in further studies.
In addition, suppressor of cytokine signaling (SOCS) proteins play a critical role in the regulation of JAK/STAT signaling pathway (Shuai and Liu, 2003). They specifically bind with key phosphorylated tyrosine residues in the activation site on JAK proteins. SOCS proteins also bind with cytokine receptors and degrade signaling molecules to negatively regulate JAK/STAT signaling (Strebovsky et al., 2012). Therefore, more studies are required to reveal the biological significance of SOCS proteins in regulation of JAK/STAT pathway and expression of ISGs in response to CSFV infection.

AUTHOR CONTRIBUTIONS
BxC, QB, and XC performed the most experiments and wrote the manuscript. J-LC, MG, LW, and BC performed some experiments and analyzed the data. SW and XC analyzed the data and revised the manuscript. J-LC designed the study, wrote and revised the manuscript. All authors read and approved the final manuscript.