Corrigendum: Phylogenetic Analyses of Shigella and Enteroinvasive Escherichia coli for the Identification of Molecular Epidemiological Markers: Whole-Genome Comparative Analysis Does Not Support Distinct Genera Designation

[This corrects the article on p. 1573 in vol. 6, PMID: 26834722.].

In the original article, there was a mistake in Table 1 as published. Our collection stock of EIEC-O152 (1) contained low level of ExPEC-O25:H16 which was sequenced in the study instead of EIEC. The corrected Table 1 appears below.
The ExPEC cluster contains the ExPEC-O25:H16 instead of the one EIEC isolate but this cluster was not discussed in the original manuscript. The NCBI accession has been updated as well.
Additionally, the text in the Phylogeny subsection in Results, first paragraph, should be written as: One hundred and seventy-one genomes were selected to encompass a large selection of EIEC strains and represent the diversity of the Shigella genus. Genomes from 35 isolates were in-house sequenced draft genomes while 136 were available in public databases (Supplementary Table 1). We used 23 isolates of SD, including a minimum of 14 serotypes, 36 SF isolates, including at least six serotypes, 32 SB isolates, covering all 20 serotypes, 26 SS isolates, 32 EIEC isolates with 15 different serotypes, 18 isolates of non-invasive E. coli composed of 14 different serotypes, two isolates of E. fergusonii. The genomes of two Salmonella isolates were used for an outgroup ( Table 1). In the original article, there was a mistake in Table 2 as published. A coding mistake led to incorrect identification of lineage-specific SNPs. We reported 404 diagnostic SNPs, but the correct count is 254. The corrected Table 2 appears below and Supplementary Table 2 with the sequences of the regions containing the diagnostic SNPs has been modified. The abstract should read "Lastly, we identified a panel of 254 single nucleotide polymorphism (SNP) markers specific to each phylogenetic cluster for more accurate identification of Shigella and EIEC." Similarly, the second line in the Lineage-Specific SNP Identification and Evaluation of Previously Described Molecular Assays for the Differentiation of Shigella and EIEC subsection in Results should read: "From 7,062 core SNPs, we found 254 SNP positions that were diagnostic for each of the clusters (Supplementary Table S2)." The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way.
The original article has been updated.

SUPPLEMENTARY MATERIAL
The    in this study. A blue cell for a particular genome indicates that both primers of the pair aligned to 95% or greater sequence identity and should therefore hybridize to yield a PCR product. The phylogenetic group designation assigned by Sahl et al. is noted next to the cluster designations we observed with these genomes.
Supplementary Figure 6 | In silico alignment of primer-probe sets described by Pavlovic et al. (2011) with genomes used in this study using BLAST. The lacY set was supposed to differentiate between Shigella (absent) and EIEC (present), while the uidA set was intended to be a positive control (present in both). BLAST identities of 92% or higher are shown with blue cells. Although PCR products are expected from a particular genome if both cells corresponding to the forward and reverse primers are highlighted in blue, the real-time PCR assay (Pavlovic et al., 2011) also require the respective probe to hybridize efficiently and therefore the respective cell to be highlighted in blue in the figure. Supplementary

Conflict of Interest Statement:
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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