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Front. Microbiol. | doi: 10.3389/fmicb.2018.00281

Involvement of BcYak1 in the regulation of vegetative differentiation and adaptation to oxidative stress of Botrytis cinerea

 Qianqian Yang1, Jianan Zhang1, Jicheng Hu1, Xue Wang1,  Binna Lv1 and  Wenxing Liang1*
  • 1Qingdao Agricultural University, China

Yak1, a member of the dual-specificity tyrosine phosphorylation-regulated protein kinases, plays an important role in diverse cellular processes in fungi. However, to date, the role of BcYak1 in Botrytis cinerea, the causal agent of gray mold diseases in various plant species, remains uncharacterized. Our previous study identified one lysine acetylation site (Lys252) in BcYak1, which is the first report of such a site in Yak1. In this study, the function of BcYak1 and its lysine acetylation site were investigated using gene disruption and site-directed mutagenesis. The gene deletion mutant ΔBcYak1 not only exhibits much lower pathogenicity, conidiation and sclerotium formation, but was also much more sensitive to H2O2 and the ergosterol biosynthesis inhibitor (EBI) triadimefon. The Lys252 site-directed mutagenesis mutant strain ΔBcYak1-K252Q (mimicking the acetylation of the site), however, only showed lower sclerotium formation and higher sensitivity to H2O2. These results indicate that BcYAK1 is involved in the vegetative differentiation, adaptation to oxidative stress and triadimefon, and virulence of B. cinerea.

Keywords: Botrytis cinerea, lysine acetylation, Yak1, vegetative differentiation, Oxidative Stress, Virulence

Received: 18 Oct 2017; Accepted: 07 Feb 2018.

Edited by:

Raffaella Balestrini, Consiglio Nazionale Delle Ricerche (CNR), Italy

Reviewed by:

Sabine Fillinger, Institut National de la Recherche Agronomique (INRA), France
Antonella Amicucci, University of Urbino, Italy  

Copyright: © 2018 Yang, Zhang, Hu, Wang, Lv and Liang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Wenxing Liang, Qingdao Agricultural University, Qingdao, China,