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Front. Microbiol. | doi: 10.3389/fmicb.2018.00347

The Effect of Carbohydrates and Bacteriocins on the Growth Kinetics and Resistance of Listeria monocytogenes

Danielle R. Balay1,  Michael G. Gänzle1 and  Lynn M. McMullen1*
  • 1Agricultural, Food and Nutritional Science, University of Alberta, Canada

The aim of this study was to determine if different carbohydrates influence the growth of Listeria monocytogenes in the presence of carnocyclin A or leucocin A. Carnobacterium maltaromaticum ATCC PTA-5313 and Leuconostoc gelidum UAL187 were used to produce carnocyclin A and leucocin A, respectively. Growth curves were modelled for 5 strains of L. monocytogenes grown in basal medium supplemented with glucose, sucrose, fructose, mannose, or cellobiose, in the presence of carnocyclin A or leucocin A. The growth of L. monocytogenes to leucocin A or carnocyclin A was influenced by carbohydrate and/or strain. Carnocyclin A inhibited the growth of L. monocytogenes more than leucocin A. Growth in media containing glucose, mannose, and fructose increased the sensitivity of some strains of L. monocytogenes to bacteriocins, while growth in cellobiose and sucrose increased the resistance of L. monocytogenes to bacteriocins, as evidenced by a shorter lag phase. Strains of L. monocytogenes developed resistance to both leucocin A and carnocyclin A, but the time to develop resistance was longer when strains are treated with carnocyclin A. Carbohydrate influences the development of resistance of L. monocytogenes to the bacteriocins, but the ability of strains to develop resistance to leucocin A or carnocyclin A differs. Results of this study indicate that carbohydrates influence the ability of L. monocytogenes to grow in the presence of bacteriocins.

Keywords: Carnocyclin A, Leucocin A, Listeria, Growth, Resistance, carbohydrate, bacteriocin

Received: 23 Oct 2017; Accepted: 13 Feb 2018.

Edited by:

Aldo Corsetti, Università di Teramo, Italy

Reviewed by:

Antonio Galvez, Universidad de Jaén, Spain
Svetoslav Todorov, University of São Paulo, Brazil  

Copyright: © 2018 Balay, Gänzle and McMullen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Lynn M. McMullen, University of Alberta, Agricultural, Food and Nutritional Science, 4-10 Agriculture Forestry Centre, Edmonton, T6G 2P5, Alberta, Canada, lmcmulle@ualberta.ca