Toxicity of Food-Grade TiO2 to Commensal Intestinal and Transient Food-Borne Bacteria: New Insights Using Nano-SIMS and Synchrotron UV Fluorescence Imaging

Titanium dioxide (TiO2) is commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties. However, a risk of intestinal barrier disruption, including dysbiosis of the gut microbiota, is increasingly suspected because of the presence of a nano-sized fraction in this additive. We hypothesized that food-grade E171 and Aeroxyde P25 (identical to the NM-105 OECD reference nanomaterial in the European Union Joint Research Centre) interact with both commensal intestinal bacteria and transient food-borne bacteria under non-UV-irradiated conditions. Based on differences in their physicochemical properties, we expect a difference in their respective effects. To test these hypotheses, we chose a panel of eight Gram-positive/Gram-negative bacterial strains, isolated from different biotopes and belonging to the species Escherichia coli, Lactobacillus rhamnosus, Lactococcus lactis (subsp. lactis and cremoris), Streptococcus thermophilus, and Lactobacillus sakei. Bacterial cells were exposed to food-grade E171 vs. P25 in vitro and the interactions were explored with innovative (nano)imaging methods. The ability of bacteria to trap TiO2 was demonstrated using synchrotron UV fluorescence imaging with single cell resolution. Subsequent alterations in the growth profiles were shown, notably for the transient food-borne L. lactis and the commensal intestinal E. coli in contact with food-grade TiO2. However, for both species, the reduction in cell cultivability remained moderate, and the morphological and ultrastructural damages, observed with electron microscopy, were restricted to a small number of cells. E. coli exposed to food-grade TiO2 showed some internalization of TiO2 (7% of cells), observed with high-resolution nano-secondary ion mass spectrometry (Nano-SIMS) chemical imaging. Taken together, these data show that E171 may be trapped by commensal and transient food-borne bacteria within the gut. In return, it may induce some physiological alterations in the most sensitive species, with a putative impact on gut microbiota composition and functioning, especially after chronic exposure.

Titanium dioxide (TiO 2 ) is commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties. However, a risk of intestinal barrier disruption, including dysbiosis of the gut microbiota, is increasingly suspected because of the presence of a nano-sized fraction in this additive. We hypothesized that food-grade E171 and Aeroxyde P25 (identical to the NM-105 OECD reference nanomaterial in the European Union Joint Research Centre) interact with both commensal intestinal bacteria and transient food-borne bacteria under non-UV-irradiated conditions. Based on differences in their physicochemical properties, we expect a difference in their respective effects. To test these hypotheses, we chose a panel of eight Grampositive/Gram-negative bacterial strains, isolated from different biotopes and belonging to the species Escherichia coli, Lactobacillus rhamnosus, Lactococcus lactis (subsp. lactis and cremoris), Streptococcus thermophilus, and Lactobacillus sakei. Bacterial cells were exposed to food-grade E171 vs. P25 in vitro and the interactions were explored with innovative (nano)imaging methods. The ability of bacteria to trap TiO 2 was demonstrated using synchrotron UV fluorescence imaging with single cell resolution. Subsequent alterations in the growth profiles were shown, notably for the transient foodborne L. lactis and the commensal intestinal E. coli in contact with food-grade TiO 2 . However, for both species, the reduction in cell cultivability remained moderate, and the morphological and ultrastructural damages, observed with electron microscopy, were restricted to a small number of cells. E. coli exposed to food-grade TiO 2 showed some internalization of TiO 2 (7% of cells), observed with high-resolution nano-secondary ion

INTRODUCTION
Engineered nanomaterials are increasingly used in numerous industrial sectors, due to their unique properties compared to their larger counterparts, provided by their nanometric dimensions and their high specific surface area (up to several hundred m 2 /g of product) (Nel et al., 2006). Among nanomaterials, titanium dioxide (TiO 2 ) is widely used, notably in the food industry, as a white coloring agent (referred to as food-grade additive E171 in the EU) for confectionery, sauces, cakes, and pastries. In the United States, the Food and Drug Administration approved the use of food-grade TiO 2 in 1966 with the stipulation that TiO 2 should not exceed 1% by weight of the food (Joint Fao/Who Expert Committee on Food Additives, 2006). In Europe, EU Directive 94/36/EC authorizes the use of E171 in processed food, without establishment of an acceptable daily intake by the Joint FAO/WHO Expert Committee on Food Additives, because intestinal TiO 2 absorption was considered very low (Efsa Panel on Food Additives and Nutrient Sources Added to Food, 2005). A sizable fraction (17-55% of total particles) of nano-sized particles (<100 nm) is produced during the manufacturing process of the powder, depending on the commercial supplier of the E171 (Weir et al., 2012;Yang et al., 2014;Bettini et al., 2017;Dorier et al., 2017). The presence of this nano-sized fraction is increasingly suspected to play a role in intestinal barrier disruption, including dysbiosis of the gut microbiota. Daily dietary intake has been estimated to be 1-2 mg TiO 2 /kg body weight (bw) for US children under 10 years of age, and 0.2-0.7 mg TiO 2 /kg bw for others (Weir et al., 2012). In Europe, estimated daily exposure levels have ranged between 0.2 and 0.4 mg/kg bw in infants and the elderly, and 5.5 and 10.4 mg/kg bw in children, depending on the exposure scenario (Efsa Panel on Food Additives and Nutrient Sources Added to Food, 2016). Recent studies report deleterious effects of E171 on the gut epithelial barrier in vitro (Faust et al., 2014;Dorier et al., 2017;Proquin et al., 2017), some of which possibly predispose the host to intestinal diseases and colorectal cancer, as shown in rodents (Urrutia-Ortega et al., 2016;Bettini et al., 2017).
Based on this background, the present study focuses on probing interactions between food-grade TiO 2 (E171) and several representative commensal intestinal and transient food-borne bacteria under non-UV irradiated conditions, by coupling different innovative (nano)imaging methods. Results were compared with those of the NM-105 (TiO 2 -NPs P25) OECD reference nanomaterial. To this end, the TiO 2 trapping properties of bacteria were evaluated using Synchrotron excitation deep ultraviolet (DUV) fluorescence imaging with single-cell resolution. Concurrently, the TiO 2 -mediated effects on bacterial growth were assessed. To gain further insight into the putative TiO 2 -driven toxicity to bacteria, morphological changes after exposure were evaluated with electron microscopy (SEM and TEM), in combination with high-resolution nanosecondary ion mass spectrometry (Nano-SIMS) to determine TiO 2 internalization.

Particle Preparation
The E171 sample was obtained from a French commercial supplier of food coloring. P25 (NM-105) nanomaterial was provided by the European Union Joint Research Centre (EU JRC) as a test material of manufactured TiO 2 nanoparticles (Aeroxyde P25) and selected by the Organization for Economic Cooperation and Development (OECD) for safety evaluation of titaniumbased nanomaterials. It displays mixed crystallinity with anatase as the predominant form (85% anatase: 15% rutile), and a mean particle diameter of 22 ± 1 nm (Rasmussen et al., 2014). The TiO 2 products, in their metrological aggregated (A) and dispersed (D) forms, were prepared according to the generic Nanogenotox dispersion protocol depicted by Jensen et al. (2011). Briefly, a 2.56 mg/mL stock suspension was prepared by pre-wetting the powder in absolute ethanol, followed by dispersion in 0.05% (w/v) BSA solution and probe sonication on ice for 27 min at 40% amplitude (Sonifier Cell Disruptor Model 250 20 kHz, Branson Ultrasonics Corporation). The stock suspensions were always prepared fresh prior to each experiment, sonicated if required, and diluted using MilliQ-grade water to the target test concentrations (0-320 µg/mL). The physicochemical characterization of the TiO 2 particles has been detailed elsewhere (Talbot et al., submitted). Spectrofluorimetric analysis was performed using a FluoroMax-4 (HORIBA Jobin Yvon, Inc., Chilly-Mazarin, France) spectrofluorometer. Measurements were performed using 10-mm path length quartz cuvettes. Fluorescence emission spectra of food-grade E171 vs. TiO 2 -NPs P25 were recorded at 20 • C at an excitation wavelength of 270 nm.

Bacterial Strains and Culture Conditions
Eight Gram-positive/Gram-negative commensal intestinal or food-borne bacterial strains, isolated from different biotopes and belonging to the species Escherichia coli, Lactobacillus rhamnosus, Lactococcus lactis (subsp. lactis and cremoris), Streptococcus thermophilus, and Lactobacillus sakei were used in this study ( Table 1). Growth kinetics experiments were performed for foodgrade E171 and TiO 2 -NPs P25 in batch cultures under non-UV irradiated conditions, with an early addition of TiO 2 (i.e., addition of TiO 2 to the culture medium before cell growth). Culture conditions are given in Table 1. For all strains, a first inoculum was obtained by adding 0.1 mL of stock culture into 5 mL of culture medium, followed by 6 h of incubation under the appropriate culture conditions ( Table 1). A volume of 0.1 mL of this preculture was then used to inoculate 10 mL of culture medium. This second preculture was incubated overnight under the same conditions until the stationary phase of growth, and then used to inoculate cultures to evaluate the effect induced by TiO 2 . A high-throughput method based on sterile microwell plates (384 wells; Greiner, Bio-one) was implemented. From the culture of each strain grown overnight, the optical density at 600 nm (OD 600nm ) was adjusted to 0.025 in appropriate culture medium. The aliquots of 70 µL from this OD 600nm -adjusted culture were deposited into wells (at least 5 wells per condition). Then, 10 µL of TiO 2 particles (E171 vs. P25), in the aggregated (A) or dispersed (D) metrological form, were added to achieve an exposure concentration of 320, 125, 62.5, or 32 µg/mL. Wells without TiO 2 particles and with only TiO 2 particles in appropriate culture medium were used as control and blank, respectively. Growth of the TiO 2exposed and non-exposed strains was then monitored every 15 min for 24 h by measuring the OD 600nm (with simultaneous stirring) using a dark-controlled and thermoregulated (30 or 37 • C depending on the strain under study) plate reader (Infinite M200 PRO, Tecan France). The strain tolerance to TiO 2 stress was assessed by determining the duration of the lag phase (h), the maximal specific growth rate µ max (h −1 ), and the final OD 600nm for each condition. Experiments were performed at least in duplicate on independently grown cultures to validate data reproducibility. In parallel, cell viability was evaluated by spreading-plating TiO 2 -exposed (test concentration of 320 µg/mL) and non-exposed bacteria on appropriate agar medium. Plates were incubated for 24 h at the appropriate temperature and the number of developed colony-forming units (CFU) was counted and expressed as CFU/mL. The viability percentage of the bacteria after TiO 2 exposure was calculated by dividing their CFU (CFU/mL) by that in the TiO 2 -free control.

Synchrotron Excitation Deep Ultraviolet (DUV) Fluorescence Imaging of Bacterial Cells After TiO 2 Exposure
Deep ultraviolet fluorescence imaging of E. coli K12 MG1655 and L. lactis IBB477 cells after TiO 2 exposure was carried out on a Zeiss Axio Observer Z-1 microscope at the DISCO beamline (Giuliani et al., 2009) of the SOLEIL synchrotron facility, as previously described for tryptophan-functionalized gold (Pajović et al., 2015) and silver nanoparticles (Dojčilović et al., 2016). Bacterial cells were cultured in appropriate medium containing TiO 2 at a concentration of 320 µg/mL (aggregated vs. dispersed forms, E171 vs. P25), collected at the stationary phase of growth and washed twice in phosphate buffered saline (5000 g, 5 min, room temperature). Thereafter, 2 µL of the washed cell suspension were placed on quartz coverslips (ESCO Optics, United States) and dried in ambient conditions for 30 min. Bacteria were first observed in bright field through a 100× Zeiss Ultrafluar objective with a 1.25 numerical aperture that requires glycerin immersion. Afterwards, the samples were illuminated by a 270-nm monochromatized synchrotron beam, which was used as the excitation source. The fluorescent signals were collected by a PIXIS 1024 BUV camera (Princeton, United States) in the spectral ranges 327-353 nm (Filter I, OMEGA Filters United States) and 420-480 nm (Filter II, OMEGA Filters, United States) during 90 s of integration time. In addition, to increase the signal to noise ratio, images were recorded with binning of the pixels (2 × 2) in µManager software (Edelstein et al., 2010), used to control the whole setup. For each condition, two independent quartz coverslips with at least three different locations, covering a minimum of 200 bacterial cells, were investigated to validate the consistency of the observations. The images were analyzed using FIJI software (ImageJ, NIH) (Schindelin et al., 2012). A set of specific FIJI macro scripts was developed to standardize the analysis (see Supplementary Material 1).

Electron Microscopy of E. coli and L. lactis Bacterial Cells Subjected to TiO 2
For scanning electron microscopy (SEM) observations, TiO 2exposed (test concentration of 320 µg/mL) and non-exposed E. coli K12 MG1655 and L. lactis IBB477 bacterial cells were harvested by centrifugation (5000 g, 5 min, room temperature), washed twice with phosphate-buffered saline, fixed and colored with a 2.5% glutaraldehyde + 0.04% Ruthenium red solution in sodium cacodylate buffer (0.1 M, pH = 7.4) for at least 4 h at 4 • C. Cells were made to adhere on poly-lysine coated coverslips and washed in distilled water prior to dehydration in graded ethanol series. Desiccation was carried out in a Leica EM CPD300 automatic critical point drier. Samples were then mounted on stubs and coated with 10 nm platinum (Leica EM MED020). Examinations were carried out on a FEI Quanta FEG 250 scanning electron microscope at a 5 kV accelerating voltage. For transmission electron microscopy (TEM), the TiO 2exposed and non-exposed cells were washed, fixed, colored as described above, then embedded in 2% Low Melting Point Agarose and washed in sodium cacodylate buffer (0.2 M) with 0.04% Ruthenium red. The post-fixation step was carried out in a mixture solution of osmium tetroxide (1%) and 0.04% Ruthenium red in sodium cacodylate buffer (0.2 M) for 1 h at room temperature. Cell dehydration was achieved through a series of graded ethanol solutions up to 70%. Infiltration was performed in an automatic microwave tissue processor Leica EM AMW, using ethanol, then acetone and several changes of Embed 812 resin/acetone mixtures, in which the concentration of resin was gradually increased to 100%. Cells were then embedded in Embed 812 (EMS) and left to polymerize 48 h at 60 • C. Polymerized samples were sectioned in 90-nm-thick slices (Ultracut Reichert) and mounted on 150 mesh collodioncoated copper grids. Before observation, sections were stained with uranyl acetate and lead citrate. TEM observations were carried out on a Hitachi HT7700 microscope, at an accelerating voltage of 80 kV.
Elemental Composition Mapmaking of E. coli and L. lactis Bacterial Cells Subjected to TiO 2 Polymerized samples, obtained as described above, were sectioned in 300-nm thick slices (Ultracut Reichert) and mounted on silicon plots (Siltronix, Archamps, France). Elemental maps were obtained by Nano-SIMS using a NanoSIMS50 instrument (Cameca, Gennevilliers, France) (Höschen et al., 2015). The surface was scanned as a matrix of 256 × 256 pixels for an area of 10 µm 2 by an energetic primary cesium ion beam with an acceleration of 8 kV and a primary current of 1.2 pA. The secondary negative ions emitted with 8 kV were filtered in mass, detected and counted simultaneously, allowing an elemental mapping of the original voxel sputtered (Georgantzopoulou et al., 2013). In these conditions, the pixel size was about 40 nm for an estimated probe size of 100 nm. The ions recorded were 12 C 14 N, 32 S, 31 P 16 O 2 , 46 Ti 16 O, and 48 Ti 16 O with a mass resolution M/ M higher than 5000. The Ti element was mapped (as TiO − cluster) on at least 100 individual cells of each sample. The distribution of the 12 C 14 N − cluster, well known by the SIMS community, was simultaneously recorded as a "fingerprint" of bacterial cells. Due to the probability of ionization and the matrix effect, i.e., large variations in the ionization yields, SIMS is not a direct quantitative technique (Behrisch and Wittmaack, 1991). However, by normalization of the trace element by the matrix element signals, a quantitative analysis (coupled with a reference sample) or a semi-quantitative analysis can be performed with an excellent accuracy (Dowsett and Collins, 1996). Unfortunately, for biological samples, it is very difficult to obtain standard samples. This explains why only the semi-quantitative approach was proposed here, based on the normalization of the signal of the titanium cluster (TiO) by the signal intensity of the main ion detected, the cluster (CN). The intensity ratio (TiO/CN) was then calculated for each cell and for all conditions (Audinot et al., 2004;Biesemeier et al., 2016). A total number between 200 and 300 cells was considered (taking into account the aggregated and dispersed TiO 2 forms). The signal intensities of the different elements were extracted from image acquisitions using OpenMIMS, a FIJI plugin developed at Harvard at the National Resource for Imaging Mass Spectrometry 1 .

Statistical Analysis
Replicates in this study were technical and biological, i.e., repeated experiments with the same and independently grown bacterial cultures, respectively. Significance was determined using one-way ANOVA corrected for multiple comparisons with Dunnett test or two-way ANOVA corrected for multiple  comparisons with Bonferroni test (GraphPad Prism software, version 4). Significance was set at * p < 0.05.

RESULTS
DUV Fluorescence Imaging of Growing L. lactis and E. coli Cells Exposed to TiO 2 For this study, Gram-positive L. lactis (IBB477 strain) and Gram-negative E. coli (K12 MG1655 strain) were chosen as representatives of transient food-borne and commensal species of the gut microbiota, respectively. DUV fluorescence imaging of L. lactis and E. coli growing bacterial cells exposed to foodgrade E171 vs. TiO 2 -NPs (P25) at 320 µg/mL was performed using a 270-nm synchrotron beam as excitation source to obtain a high TiO 2 /autofluorescence ratio. First, food-grade E171 particles in aggregated (A) and dispersed (D) forms, like their P25 counterparts, could be excited in the DUV range at 270 nm and displayed fluorescence emission spectra with characteristic peaks of TiO 2 (Serpone et al., 1995), i.e., a maximum of fluorescence emission around 390 nm with fluorescence intensity remaining at high levels up to 470 nm ( Figure 1A). Interestingly, whatever the type of TiO 2 , the maximum of the fluorescence emission increased as the size of TiO 2 particles decreased. Next, interactions between bacteria and TiO 2 were visualized based on the differences in fluorescence emission spectra between bacteria and TiO 2 particles. The signals in the [327-353 nm] range originate from cell autofluorescence, mainly due to tryptophan fluorescence [peaking at around 340 nm (Jamme et al., 2010;Kašèáková et al., 2012)], which is responsible for the characteristic optical properties of many proteins (Lakowicz, 2006), whereas, as seen above (Figure 1A), emission from TiO 2 -exposed bacterial cells in the [420-480 nm] range is attributed to the fluorescence of TiO 2 particles, located inside and/or on the surface of the bacterial cells. Overlays of the fluorescence images acquired within [327-353 nm] and [420-480 nm] detection ranges are given in Figure 1B for L. lactis and E. coli exposed to foodgrade TiO 2 (E171-A) and TiO 2 -NPs P25 (P25-D). We clearly demonstrated that DUV fluorescence imaging allows for noninvasive monitoring of TiO 2 without any artificial labeling at single-cell resolution. In fact, whatever the TiO 2 type, a TiO 2 /cell co-localization was observed for both L. lactis and E. coli (Figures 1Ba-d).

TiO 2 Toxicity to Growing Bacteria
In order to evaluate the impact of co-localization between the selected bacteria and TiO 2 on their physiological profiles, growth kinetics were characterized in the presence of foodgrade E171 or TiO 2 -NPs (P25) in the aggregated vs. dispersed forms at concentrations ranging from 32 to 320 µg/mL. These growth kinetics patterns were compared to the control FIGURE 3 | Viability of bacterial cells after TiO 2 exposure. Viable counts (in % of the control) of L. lactis IBB477 (A) and E. coli K12 MG1655 (B) subjected to TiO 2 at 320 µg/mL in its dispersed form D for food-grade E171 (light orange) vs. TiO 2 -NPs P25 (light green), and sampled at the end of the culture. Data are the means ± SEM. Significance was determined using one-way ANOVA corrected for multiple comparisons with Dunnett test: ( * ) indicates p < 0.05 compared to control conditions with no TiO 2 added. FIGURE 4 | Scanning electron microscopy (SEM) images of bacterial cells after TiO 2 exposure. (A,D) non-exposed growing bacterial cells, (B,E) growing bacterial cells exposed to E171-A 320 µg/mL, and (C,F) growing bacterial cells exposed to E171-D 320 µg/mL; (A-C) L. lactis IBB477, (D-F) E. coli K12 MG1655. White arrows indicate damaged bacterial cells.
condition without any TiO 2 added. Results are displayed in Figure 2.
For L. lactis IBB477 exposed to E171 and P25, whatever the metrological form, bacterial growth was close to that of the control until a TiO 2 concentration of 62.5 µg/mL. For the concentration of 125 µg/mL, significant alterations occurred especially for food-grade TiO 2 in its dispersed form (Figures 2A,B and Supplementary Material 2) and, for the highest concentration of 320 µg/mL, growth kinetics were substantially affected under all tested conditions (p < 0.001, Figures 2A,B and Supplementary Material 2). For E. coli K12 MG1655, TiO 2 -induced inhibition of bacterial growth was also clearly demonstrated, with a more pronounced dosedependent manner than for L. lactis (Figures 2C,D). In particular, for food-grade TiO 2 at 320 µg/mL, no growth of E. coli occurred throughout the entire experiment, i.e., 20 h of culture ( Figure 2D).
These effects on L. lactis IBB477 and E. coli K12 MG1655 were measured by the variations in three growth-related parameters, i.e., lag time (delay in the onset of growth), specific growth rate (µ max ) and final biomass (OD 600nm ) between TiO 2 -exposed and non-exposed cells at the highest concentration tested (320 µg/mL). Results for E171 vs. TiO 2 -NPs P25 in the dispersed form are displayed in Table 2. For L. lactis IBB477, growth parameters were drastically affected, depending on the type of TiO 2 . For E171 and P25, respectively, lag time increased by a factor of 4.3 and 1.7, µ max decreased by a factor of 2.4 and 2.3, and the final biomass was reduced by a factor of 6 and TABLE 2 | Sensitivity of all bacterial strains to Titanium dioxide (TiO 2) in its dispersed form (P25-D in light green and E171-D in light orange).

Lag time (h)
Specific growth rate µ max (h −1 ) Final biomass (OD 600nm ) NS * * * * * * * * * * Three growth parameters have been considered: lag time (h), specific growth rate µ max (h −1 ) and final biomass (OD 600nm ). Ratios have been calculated with the mean values obtained for the maximal TiO 2 concentration (320 µg/mL) vs. control conditions (no TiO 2 added); decrease, increase, NA, not available. Significance was determined for each growth parameter considering the overall P25-D-and E171-D-treated strains (except E. coli K12 MG1655) using one-way ANOVA corrected for multiple comparisons with Dunnett test: ( * * ) indicates p < 0.01 compared to control conditions with no TiO 2 added (NS, not significant).
3. After exposure of E. coli K12 MG1655 to P25-D, growth parameters were impacted as well. For E171-D, it was not possible to obtain quantitative data since no bacterial growth occurred at all. These deleterious effects were confirmed for other strains of the same species and were highest for foodgrade TiO 2 ( Table 2). Among the E. coli strains tested, K12 MG1655 was shown to be the most sensitive. Furthermore, for the other commensal intestinal and food-borne bacteria studied (L. rhamnosus, L. sakei, S. thermophilus), TiO 2 -induced inhibition of bacterial growth was systematically observed [lag time: p < 0.01 for E171-D (p > 0.05 for P25-D); µ max and final biomass: p < 0.01 for E171-D and P25-D; Table 2].
The effects of TiO 2 in its dispersed form (E171-D and P25-D) at 320 µg/mL on L. lactis IBB477 and E. coli K12 MG1655 were assessed using the plate count method for TiO 2 -exposed vs. non-exposed bacteria sampled at the end of the culture. A non-significant loss in cell cultivability was observed for L. lactis IBB477 ( Figure 3A). However, for E. coli K12 MG1655, a moderate albeit significant reduction in viability counts (50% decrease, p < 0.05) occurred for food-grade TiO 2 ( Figure 3B). This is consistent with the above findings on the impact of TiO 2 on the growth kinetics of L. lactis and E. coli.
Electron Microscopy of L. lactis and E. coli Cells Exposed to TiO 2 The interaction of L. lactis IBB477 and E. coli K12 MG1655 with TiO 2 and the subsequent changes in cell morphology and structure were investigated with SEM and TEM. Figure 4 shows representative SEM images obtained for both species with or without exposure to food-grade E171 in its aggregated and dispersed forms at 320 µg/mL. For L. lactis IBB477, untreated bacteria appeared as intact cocci with no evidence of cell wall rupture and collapse ( Figure 4A). In contrast, after TiO 2 exposure, a small number of bacteria (see white arrows in Figures 4B,C) showed extensive cell wall damage and leaking out of intracellular components, probably causing cell lysis. For untreated E. coli K12 MG1655, the surface structure of rod-shaped bacteria was almost intact, with regular wrinkles at nano-scale resolution ( Figure 4D). After treatment with food-grade TiO 2 , in a small number of cases, cells became twisted and rougher. Regular wrinkles were damaged and groove-like rifts appeared on the surface. The cell morphology was substantially altered, for instance cells became wider and shorter, or completely deformed (see white arrows in Figures 4E,F). For TiO 2 -NPs P25, the same changes were observed, although to a lesser extent (data not shown). Figure 5 shows TEM images of L. lactis IBB477 and E. coli K12 MG1655 bacterial cells untreated vs. treated with foodgrade E171 or TiO 2 -NPs P25 in aggregated and dispersed forms at 320 µg/mL. Non-exposed bacteria remained intact with unimpaired morphology and ultrastructure, as seen in Figure 5A,D for L. lactis and E. coli, respectively. After TiO 2 exposure, particle aggregates were observed attaching to the bacterial cells, but some were also present away from the cells. Of note, the primary particle size of food-grade E171 was higher than that of P25, which is consistent with previous findings Bettini et al., 2017). Regarding the consequences of TiO 2 exposure on L. lactis IBB477, in a few cases, cells were completely lysed and appeared as "ghosts" (Figures 5B,C). In some E. coli K12 MG1655 cells, the TiO 2 treatment induced cell distortion, plasmolysis, and cell wall and membrane damages (Figures 5E-G). Observations made with SEM and TEM were consistent between each other, both showing morphological and ultrastructural changes restricted to a limited number of bacterial cells. Interestingly, food-grade E171 particles were found tightly associated to the cell surface, causing some deformation at the contact zone (Figures 5H,I).

TiO 2 Internalization Within L. lactis and E. coli Cells
The conventional analytical capabilities associated with electron microscopy (EM), such as the Energy Dispersive Spectroscopy (EDS) or Electron Energy-Loss Spectroscopy (EELS), are not sensitive enough for the characterization of biological samples containing trace elements, with a detection limit of 0.1 at %. This FIGURE 5 | Transmission electron microscopy (TEM) images of bacterial cells after TiO 2 exposure. (A-C) growing L. lactis IBB477 bacterial cells: (A) non-exposed, (B) exposed to P25-A 320 µg/mL and (C) exposed to E171-D 320 µg/mL; (D-I) growing E. coli K12 MG1655 bacterial cells: (D) non-exposed, (E) exposed to P25-A 320 µg/mL, (F) exposed to P25-D 320 µg/mL, (G,H) exposed to E171-A 320 µg/mL and (I) exposed to E171-D 320 µg/mL. explains why it was not possible to use EM to accurately assess whether or not TiO 2 internalization occurred in bacterial cells. High-resolution Nano-SIMS was used, which combines high lateral resolution, high mass resolution and a high detection limit (few ppm per voxel) (Sangely et al., 2014;Höschen et al., 2015;Wirtz et al., 2015). The methodology developed and presented in Figure 6A allowed us to obtain semi-quantitative information at the subcellular scale (<100 nm) on the intracellular localization of Ti for L. lactis IBB477 and E. coli K12MG1655 exposed to E171 (E171-A and E171-D) or TiO 2 -NPs P25 (P25-A and P25-D). The intensity ratios between the trace element and the matrix element signals (TiO/CN) are displayed in Figures 6Ba,b for L. lactis and E. coli, respectively. The percentage of TiO/CNpositive bacterial cells is also given in Table 3. For L. lactis cells, no TiO 2 internalization was observed (Figure 6Ba and Table 3). In contrast, for E. coli, about 7% of bacterial cells internalized TiO 2 particles (Table 3), with the highest levels achieved with food-grade TiO 2 (Figure 6Bb). This corresponds to the altered growth kinetics, and morphological and ultrastructural changes depicted above.

DISCUSSION
In this study, Nano-SIMS-and synchrotron UV fluorescencebased imaging was used in combination with electron microscopy to provide valuable mechanistic insights into the toxicity of food-grade TiO 2 (additive E171) to commensal intestinal and transient food-borne bacteria. The presence of a nano-sized fraction in this additive, as shown by Yang et al. (2014) and Dudefoi et al. (2017b) is increasingly suspected to play a role in disruption of intestinal homeostasis and development of gut microbiota dysbiosis. The microbiota, an important gut player, is rarely explored in food nanotoxicology (Fröhlich and Fröhlich,FIGURE 6 | High-resolution nano-secondary ion mass spectrometry (Nano-SIMS) chemical imaging of bacterial cells after TiO 2 exposure. (A) Methodology for the extraction of the SIMS intensities for each individual cell. From the SIMS CN image acquisition, by auto sharpening, each cell is individualized. The mask obtained is applied on image acquisition, allowing to extract the total SIMS intensities for each cell and for all the ions recorded; (B) Distribution of the TiO signal normalized by the CN signal among the bacterial population for (a) L. lactis IBB477 and (b) E. coli K12 MG1655 bacterial cells non-exposed (black hatched) and exposed to P25-A (dark green hatched), P25-D (light green hatched), E171-A (red hatched) or E171-D (yellow hatched). For each species a representative image is given with cells (in red) exposed to E171-A (in green) at 320 µg/mL. White arrows indicate internalized TiO 2 particles. Samples were fixed, embedded and ultra-sectioned. Scale bar corresponds to 2 µm. 2016; Fröhlich and Roblegg, 2016;Mercier-Bonin et al., 2016;Pietroiusti et al., 2016), apart from two recent studies which focus on E171 vs. P25 (Dudefoi et al., 2017a;Waller et al., 2017). Both studies were based on an in vitro colon model, inoculated with a gut microbial community from a healthy donor (Waller et al., 2017) or the defined anaerobic human gut bacterial community MET-1 (Dudefoi et al., 2017a). Depending on the conditions under study (source and characteristics of foodgrade TiO 2 , concentration of TiO 2 particles, exposure duration, model colon reactor design), different results were obtained. Food-grade TiO 2 showed little impact on bacterial respiration, fatty acid profiles and phylogenetic composition (Dudefoi et al., 2017a); in contrast, changes to the microbial composition (i.e., inhibition of the control-induced shift in microbial composition from Proteobacteria to Firmicutes phyla) were found, together with some alterations of biochemical responses like a decrease in colonic pH (Waller et al., 2017). These differences in gut ecosystem functioning may be explained, at least to some extent, by direct interaction between TiO 2 particles and bacteria (Jiang et al., 2009;Waller et al., 2017).
To address the question of TiO 2 /bacteria interactions and related toxicity, we selected a panel of Gram-negative/Grampositive bacterial species from diverse origins and biotopes, including commensal and transient food-borne bacteria, exposed to non-UV-irradiated food-grade TiO 2 (E171) vs. TiO 2 -NPs (P25). Apart from commensal bacteria, transient food-borne bacteria may also be in contact with TiO 2 through ingested foods and/or during their passage through the gut, where they can also impact the resident microbiota and thus possibly the health of the host (Veiga et al., 2014;Derrien and van Hylckama Vlieg, 2015;Zhang et al., 2016). To date, the only study dealing with interactions between exogenously applied bacteria and nano-sized TiO 2 relates to the food-borne pathogen Listeria monocytogenes (Ammendolia et al., 2014). In the present study, bacterial growth was inhibited by TiO 2 in all selected bacteria (E. coli, L. lactis, L. rhamnosus, L. sakei, and S. thermophilus), particularly by food-grade TiO 2 . Based on these data, we focused on E. coli as a representative of Gram-negative commensal intestinal bacteria  and widely used as a model to characterize the antibacterial properties of nanosized TiO 2 (Liu et al., 2010;Kumar et al., 2011;Zhukova et al., 2012;Erdem et al., 2015;Sohm et al., 2015;Planchon et al., 2017). Indeed, E. coli is one of the first bacterial species to colonize the intestine and remains the predominant aerobic organism in the gastrointestinal tract into adulthood (Tenaillon et al., 2010). Additionally, we used the lactic acid bacterium L. lactis as a model of Gram-positive food-borne bacteria. The strain L. lactis IBB477 we studied possesses adhesive properties toward components of the intestinal mucosa (Radziwill-Bienkowska et al., 2014, which can be partly responsible for the prolonged and/or enhanced contact between these exogenously applied bacteria and the host. The percentage of TiO/CN-positive cells is given for L. lactis IBB477 and E. coli K12 MG1655, and for TiO 2 -NPs P25 and food-grade E171 (sum of aggregated and dispersed forms).
In our study, imaging with DUV fluorescence was performed. Under these conditions, the photoluminescence of TiO 2 particles enables tracking their accumulation in bacteria with a higher spatial resolution compared to conventional optical methods (Jamme et al., 2010). In previous works, this technique has been successfully employed to probe the distribution of antimicrobials in Enterobacter aerogenes (Kašèáková et al., 2012) as well as the accumulation of tryptophan-functionalized gold and silver nanoparticles in E. coli (Pajović et al., 2015;Dojčilović et al., 2016). Here, DUV fluorescence imaging with singlecell resolution revealed TiO 2 /cell co-localization by enabling to distinguish between the fluorescent signal pertaining to TiO 2 particles and the autofluorescence of treated E. coli or L. lactis bacterial cells. As further shown by scanning and transmission electron microscopy, in a few cases, such TiO 2 /cell co-localization resulted in morphological and ultrastructural damages to both L. lactis and E. coli. One part of the bacterial population strongly interacted with food-grade E171 and to a lesser extent with TiO 2 -NPs P25 while the other part was totally free of TiO 2 on the cell surface. This is completely in line with the findings of Planchon et al. (2017) on E. coli exposed to TiO 2 -NPs, which underlined that, which underlined that such heterogeneities, probably due to physiological variability, could account for the different metabolomic and proteomic responses observed.
To gain further insight into the mechanisms involved, the internalization of TiO 2 in bacterial cells was probed using Nano-SIMS imaging. This method, initially developed for isotopic measurement at the single-cell level Li et al., 2008), was first applied to elemental silver-exposed E. coli (Saulou-Bérion et al., 2015). In fact, image acquisition at the subcellular scale (<100 nm) allowed for determination of the cell shape, thanks to the CN − signal, and the intracellular localization of Ti (TiO − signal). The ratio between intensities of the minor element TiO, and the major matrix ions CN, was measured per individual bacterium, which gives semi-quantitative values on the ability of the Ti element to enter the cells, for all experimental conditions tested. In contrast with L. lactis, for approximately 7% of E. coli cells exposed to food-grade TiO 2 , internalization occurred. These findings correspond to toxicity results of E171-exposed bacteria, where alterations in growth kinetics and viability reduction were greatest for E. coli. This is the first in vitro study demonstrating the bacterial toxicity of food-grade TiO 2 , which differs from the P25 OECD reference nanomaterial, probably due to its specific physicochemical properties Dudefoi et al., 2017b). Previous works under similar non-UV-irradiated conditions mainly focused on the effects of P25 or "P25like" TiO 2 -NPs, generally tested in E. coli. Depending on the experimental conditions (size of TiO 2 -NPs, exposure regime), E. coli mortality was in the range between 20 and 80% (Erdem et al., 2015;Sohm et al., 2015;Planchon et al., 2017) with Gram-negative bacteria (E. coli) more susceptible than Grampositive ones (Bacillus subtilis) (Erdem et al., 2015), which is fully consistent with our results. In other studies, morphological damages were depicted, together with probable internalization even though only qualitative observations were provided at this stage (Liu et al., 2010;Lin et al., 2014). TiO 2 -NPs also induced lipid peroxidation and disruption of cellular respiration (Erdem et al., 2015), membrane depolarization, loss of integrity and fluidity, and higher cell permeability (Liu et al., 2010;Sohm et al., 2015). These alterations on E. coli were further dissected at the transcriptomic, proteomic and metabolomic levels to identify biomarkers (Sohm et al., 2015;Planchon et al., 2017). Moreover, TiO 2 -NPs were found to induce both DNA damage and oxidative stress (Kumar et al., 2011), even though in the later study of Sohm et al. (2015), very few genes/proteins linked to oxidative stress were deregulated. Although beyond the scope of the present study, it would be valuable to further decipher the primary mode of action of food-grade TiO 2 on E. coli and other commensal intestinal bacteria, as well as transient food-borne bacteria.
To conclude, our study provides new insights on interactions under non-UV irradiated conditions between TiO 2 (food-grade E171 vs. TiO 2 -NPs P25) and bacteria of the gut microbiota (e.g., E. coli) or ingested with food (e.g., L. lactis) using innovative biophysics-based methods. We showed that TiO 2 can be trapped by bacteria, which in turn induces moderate bacterial toxicity; E. coli exposed to food-grade TiO 2 showed the most striking effects. However, especially during chronic exposure, the continuing presence of TiO 2 within the intestine may reasonably be expected to produce substantial changes in the composition and the metabolic activity of microbiota, by its influence on the most sensitive bacteria. Furthermore, alterations in gut microbiota composition have been shown to occur in response to microbial challenges, variations in diet, and intestinal disorders. Deleterious effects of the food additive E171 might thus be potentiated by these factors. The TiO 2 -trapping capacity of foodborne bacteria could also be exploited to improve conditions for a healthy gut. All these areas of research should be further considered in risk assessment of dietary nanoparticles.

ACKNOWLEDGMENTS
The authors wish to thank Marie-Pierre Duviau (LISBP, Toulouse, France) for her technical assistance in bacterial cell cultures. DUV imaging of TiO 2 -exposed vs. non-exposed bacteria was performed at the DISCO beamline of Synchrotron SOLEIL (France) as part of the research project number 20131260. They wish to thank Florian Chain, Claire Cherbuy, Françoise Rul (MICALIS, Jouy-en-Josas, France), and Patrick Grysan (LIST, Belvaux, France) for their technical help and fruitful discussions on Synchrotron SOLEIL experiments and NanoSIMS50 experiments, respectively. Hervé Robert (ToxAlim, Toulouse, France) is gratefully thanked for his help in the data statistical analysis and Cezary Bieńkowski (Software Engineer, Warsaw, Poland) for his contribution to writing the FIJI macro scripts for data analysis after DUV fluorescence imaging. The Synchrotron SOLEIL for provision of synchrotron radiation facilities, the DISCO beamline staff, especially Bertrand Cinquin, and the Biology support lab, are also gratefully thanked.