AUTHOR=Flannagan Ronald S. , Heinrichs David E. TITLE=A Fluorescence Based-Proliferation Assay for the Identification of Replicating Bacteria Within Host Cells JOURNAL=Frontiers in Microbiology VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.03084 DOI=10.3389/fmicb.2018.03084 ISSN=1664-302X ABSTRACT=
Understanding host pathogen interactions is paramount to the development of novel antimicrobials. An important facet of this pursuit is the accurate characterization of pathogen replication within infected host cells. Here we describe the use of a fluorescence-based proliferation assay to identify intracellular populations of replicating bacteria at the subcellular level. Using