Front. Microbiol. Frontiers in Microbiology Front. Microbiol. 1664-302X Frontiers Media S.A. 10.3389/fmicb.2019.00021 Microbiology Correction Corrigendum: Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology Deng Yiqin 1 2 Su Youlu 1 Liu Songlin 3 Guo Zhixun 1 Cheng Changhong 1 Ma Hongling 1 Wu Jinjun 1 Feng Juan 1 * Chen Chang 2 4 * 1Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China 2Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China 3Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China 4Xisha/Nansha Ocean Observation and Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China

Edited and reviewed by: Eric Altermann, AgResearch, New Zealand

 *Correspondence: Juan Feng juanfeng@scsfri.ac.cn Chang Chen chen.chang@scsio.ac.cn

This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology

 31 01 2019 2019 10 21 14 12 2018 09 01 2019 Copyright © 2019 Deng, Su, Liu, Guo, Cheng, Ma, Wu, Feng and Chen. 2019 Deng, Su, Liu, Guo, Cheng, Ma, Wu, Feng and Chen

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 A Corrigendum on Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology by Deng, Y., Su, Y., Liu, S., Guo, Z., Cheng, C., Ma, H., et al. (2018). Front. Microbiol. 9:2394. doi: 10.3389/fmicb.2018.02394 small non-coding RNAs srvg23535 Vibrio alginolyticus identification Phenotype MicroArray technology

In the published article, there was an error in affiliation 1. Instead of “Ministry of Agriculture” the correct name of the ministry is “Ministry of Agriculture and Rural Affairs”.

In Table 1, the references for “53813,” “GEB88,” and “pSW7848,” were incorrectly written as “This lab.” It should be “Le Roux et al., 2007,” “Nguyen et al., 2018,” and “Val et al., 2012,” respectively. Additionally, the intermediate host Escherichia coli strain was named as “GEB802,” but should be “53813.”

The corrected Table 1 appears below.

Strains and plasmids used in this study.

Strains or plasmids Relevant characteristics Sources
V. alginolyticus
ZJ-T Apr (ampicillin resistant), translucent/smooth variant of wild strain ZJ-51 (Xiaochun et al., 2017); isolated from diseased Epinephelus coioides off the Southern China coast Chang et al., 2009
ZJ-T-Δsrvg23535 Apr; ZJ-T carrying an deletion of srvg23535 This study
E. coli
Π3813 Emrr, Tcr, lacIQ, thi1, supE44, endA1, recA1, hsdR17, gyrA462, zei298::tn10[Tc], ΔthyA:: (erm-pir116); the intermediate host of suicide vector pSW7848 Le Roux et al., 2007
GEB883 Eryr, Tetr, WT E. coli K12 ΔdapA::erm pir RP4-2 ΔrecA gyrA462, zei298::Tn10; donor strain for conjugation Nguyen et al., 2018
Plasmids
pSW7848 Cmr; suicide vector with an R6K origin, requiring the Pir protein for its replication, and the ccdB toxin gene Val et al., 2012
pSW7848-Δsrvg23535 Cmr; pSW848 containing the mutant allele of Δsrvg23535 This study

A correction has also been made to the MATERIALS AND METHODS, Bacterial Strains, Plasmids, and Growth Conditions and Gene Disruption, paragraph one:

“To generate the sRNA disruptant, the sequence from 46 bp before the 5′ end to 2 bp after the 3′ end was deleted from the chromosome of V. alginolyticus ZJ-T. The deletion was constructed by homologous recombination as described before with some modification (Yiqin et al., 2016). Briefly, two flanking fragments of srvg23535 (Figure 1A) were amplified with two pairs of primers, srvg23535-UP-F and -R and srvg23535-DOWN-F and -R respectively, and the linearized pSW7848 was amplified with pSW7848-F and -R (Supplementary Table 1). srvg23535-UP-F and srvg23535-DOWN-R contained overlapping extensions with pSW7848-R and -F, respectively, and srvg23535-UP-R contained overlapping extensions with srvg23535-DOWN-F. The two flanking fragments were further assembled into the linearized pSW7848 by using a ClonExpress Multis One Step Cloning Kit (Vozyme, China), generating the recombinant plasmid pSW7848-Δsrvg23535 comprising the 1,084 bp upstream and 1,105 bp downstream regions of srvg23535 (Table 1), using E. coli Π3813 as an intermediate host. The recombinant plasmid was transferred by conjugation from strain GEB883 (Table 1) to V. alginolyticus ZJ-T before allelic exchange as described above. The sRNA disruptant was then confirmed by sequencing and the strain was named ZJ-T-Δsrvg23535 (Figure 1 and Table 1).”

The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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