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Front. Microbiol. | doi: 10.3389/fmicb.2019.00295

A Major Facilitator Superfamily Peptide Transporter from Fusarium oxysporum influences bioethanol production from lignocellulosic material.

  • 1School of Biology and Environmental Science, University College Dublin, Ireland
  • 2Beltsville Agricultural Research Center, Agricultural Research Service (USDA), United States
  • 3Department of Crop Science, Teagasc, The Irish Agriculture and Food Development Authority, Ireland

Fusarium oxysporum is a leading microbial agent in the emerging consolidated bioprocessing (CBP) industry owing to its capability to infiltrate the plant’s lignin barrier and degrade complex carbohydrates to value-added chemicals such as bioethanol in a single step. Membrane transport of nutrients is a key factor in successful microbial colonisation of host tissue. This study assessed the impact of a peptide transporter on F. oxysporum’s ability to convert lignocellulosic straw to ethanol. We characterized a novel F. oxysporum peptide transporter (FoPTR2) of the dipeptide/tripeptide transporter (PTR) class. FoPTR2 represents a novel transporter with high homology to the Trichoderma sp. peptide transporters ThPTR2 and TrEST-AO793. Its expression level was highly activated in nitrogen-poor environments, which is a characteristic of PTR class peptide transporters. Overexpression and post-translational gene silencing of the FoPTR2 in F. oxysporum affected the peptide transport capacity and ethanol yielded from a both a wheat straw/bran mix and glucose. Thus, we conclude that it FoPTR2 plays a role in the nutrient acquisition system of F. oxysporum which serves to not only enhance fungal fitness but also CBP efficacy.

Keywords: bioethanol, Fusarium, Peptide transporter, Consolidated Bioprocessing, Lignocellulose

Received: 11 Jun 2018; Accepted: 04 Feb 2019.

Edited by:

Hector Mora Montes, Universidad de Guanajuato, Mexico

Reviewed by:

Claudio Altomare, Italian National Research Council (CNR), Italy
Arindam Kuila, Department of Bioscience and Biotechnology, Banasthali University, India  

Copyright: © 2019 Nugent, Ali, Mullins and Doohan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Shahin S. Ali, Beltsville Agricultural Research Center, Agricultural Research Service (USDA), Beltsville, United States,