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Front. Microbiol. | doi: 10.3389/fmicb.2019.00300

Probiotic properties of Enterococcus isolated from artisanal dairy products

  • 1Agricultural Research, Education and Extension Organization (AREEO), Iran

The present study focused on probiotic characterization and safety evaluation of Enterococcus bacteria from different artisanal dairy products. Morphological properties, acid and bile tolerance, antimicrobial activities, PCR amplification of known enterocin genes, exopolysaccharide production, auto-aggregation, co-aggregation, cell surface hydrophobicity, adhesion to human intestinal cells, cholesterol removing ability, hemolytic and β-galactosidase activities, bile salt hydrolysis, resistance to antibiotics and detection of virulence factors were evaluated. All the isolates exhibited inhibitory activity against several food spoilage bacteria and food-borne pathogens, including Shigella flexneri, Staphylococcus aureus, Listeria monocytogenes, Yersinia enterocolitica, Klebsiella pneumoniae, Escherichia coli and Bacillus subtilis. The Enterococcus isolates showed cholesterol lowering and pathogen inhibition properties. Hydrophobicity and auto-aggregation results indicated strong attachment capacity of the isolates. The PCR results indicated the presence of at least one enterocin structural gene in all the tested strains. The Enterococcus isolates were further evaluated regarding their safety properties and functional features. The isolates were susceptible to vancomycin, gentamycin and chloramphenicol. The results of PCR amplification revealed that all the tested isolates harbored none of the tested virulence genes. This study revealed that the Enterococcus isolates, especially E. durans ES11, ES20 and ES32, might be excellent candidates for production of functional foods to promote health benefits.

Keywords: Low cholesterol, Antimicrobial activity, Safety evaluation, Enterococcus as probiotics, Virulence Factors

Received: 26 Sep 2018; Accepted: 04 Feb 2019.

Edited by:

Learn-Han Lee, Novel Bacteria and Drug Discovery Research Group (NBDD), Monash University Malaysia, Malaysia

Reviewed by:

Sergio E. Pasteris, Universidad Nacional de Tucumán, Argentina
Konstantinos Papadimitriou, Agricultural University of Athens, Greece  

Copyright: © 2019 NAMI, Vaseghi Bakhshayesh, Mohammadzadeh Jalaly, Lotfi, Eslami and Hejazi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Mohammad Amin Hejazi, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran, aminhejazi@yahoo.com