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This article is part of the Research Topic

Host Genetics in Viral Pathogenesis and Controls

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.00636

Elucidating the host interactome of EV-A71 2C reveals viral dependency factors

Ye Li1,  Xia Jian1,  Peiqi Yin1, Guofeng Zhu2 and  Leiliang Zhang1*
  • 1MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, China
  • 2Institute of Biophysics (CAS), China

Viral protein 2C plays a critical role in EV-A71 replication. The discovery of 2C binding proteins will likely provide potential targets to treat EV-A71 infection. Here, we provide a global proteomic analysis of the human proteins that interact with the EV-A71 2C protein. TRIM4, exportin2 and ARFGAP1 were validated as 2C binding partners. Further functional studies revealed that TRIM4, exportin2 and ARFGAP1 were novel host dependency factors for EV-A71. Moreover, enteroviruses 2C family proteins interacted with exportin2 and ARFGAP1. In conclusion, our study provides a cellular interactome of the EV-A71 2C and identifies the proviral roles of TRIM4, exportin2 and ARFGAP1 in EV-A71 infection.

Keywords: EV-A71, 2C, TRIM4, exportin2, ArfGAP1

Received: 13 Jul 2018; Accepted: 13 Mar 2019.

Edited by:

Ju-Tao Guo, Baruch S. Blumberg Institute, United States

Reviewed by:

Ping Zhao, Second Military Medical University, China
Ke Lan, State Key Laboratory of Virology, Wuhan University, China  

Copyright: © 2019 Li, Jian, Yin, Zhu and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Leiliang Zhang, MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China, armzhang@hotmail.com