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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02360

Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov.

Cristina Merla1, 2,  Carla Rodrigues3,  Virginie Passet3,  Marta Corbella1, Harry A. Thorpe4,  Teemu V. Kallonen5, 6,  Zhiyong Zong7,  Piero Marone1,  Claudio Bandi8, 9,  Davide Sassera10, Jukka Corander5, 6, 11, Edward J. Feil4 and  Sylvain Brisse3*
  • 1San Matteo Hospital Foundation (IRCCS), Italy
  • 2Department of Microbiology and Virology, Faculty of Medicine, University of Pavia, Italy
  • 3Laboratory for Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, France
  • 4Milner Centre for Evolution, University of Bath, United Kingdom
  • 5Wellcome Sanger Institute (WT), United Kingdom
  • 6Department of Biostatistics, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
  • 7Center of Infectious Diseases, West China Hospital, Sichuan University, China
  • 8Department of Biosciences, Faculty of Science and Technology, University of Milan, Italy
  • 9Pediatric Research Center Romeo and Enrica Invernizzi Hospital, Italy
  • 10Department of Biology and Biotechnology Lazzaro Spallanzani, University of Pavia, Italy
  • 11Helsinki Institute for Information Technology (HIIT), Finland

Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhoea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4, Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), Klebsiella oxytoca (Ko2), K. huaxiensis (Ko8) and K. grimontii (Ko6). The average nucleotide identity of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human faeces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4 and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1T (CIP 111695T, DSM 109531T) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T, DSM 109530T) as type strain. Strains of K. spallanzanii were isolated from human urine, cow faeces and farm surfaces, while strains of K. pasteurii were found in faecal carriage from humans, cows and turtles.

Keywords: Klebsiella, Taxonomy, phylogeny, Classification, Biochemical characterisation, MALDI - TOF

Received: 05 Jul 2019; Accepted: 27 Sep 2019.

Copyright: © 2019 Merla, Rodrigues, Passet, Corbella, Thorpe, Kallonen, Zong, Marone, Bandi, Sassera, Corander, Feil and Brisse. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Sylvain Brisse, Laboratory for Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France, sbrisse@pasteur.fr