TY - JOUR AU - Feyereisen, Marine AU - Mahony, Jennifer AU - Neve, Horst AU - Franz, Charles M. A. P. AU - Noben, Jean-Paul AU - O’Sullivan, Tadhg AU - Boer, Viktor AU - van Sinderen, Douwe PY - 2019 M3 - Original Research TI - Biodiversity and Classification of Phages Infecting Lactobacillus brevis JO - Frontiers in Microbiology UR - https://www.frontiersin.org/articles/10.3389/fmicb.2019.02396 VL - 10 SN - 1664-302X N2 - Lactobacillus brevis is a lactic acid bacterium that is known as a food and beverage spoilage organism, and more specifically as a beer-spoiler. Phages of L. brevis have been described, but very limited data is available regarding temperate phages of L. brevis. Temperate phages may exert benefits to the host, while they may also be employed to combat beer spoilage. The current study reports on the incidence of prophage sequences present in nineteen distinct L. brevis genomes. Prophage induction was evaluated using mitomycin C exposure followed by genome targeted-PCR, electron microscopy and structural proteome analysis. The morphological and genome sequence analyses revealed significant diversity among L. brevis prophages, which appear to be dominated by members of the Myoviridae phage family. Based on this analysis, we propose a classification of L. brevis phages into five groups. ER -