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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02673

Identification of novel putative bacterial feruloyl esterases from anaerobic ecosystems by use of whole-genome shotgun metagenomics and genome binning

  • 1Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Sweden
  • 2Stockholm University, Sweden

Feruloyl esterases (FAEs) have a wide range of applications in industry. They can be used to reduce recalcitrance of lignocellulosic biomass to enzymatic degradation and thereby to enhance utilization of lignocellulosic biomass in biorefineries or in animal feeding. In addition, ferulic acid, a product of FAE activity, has applications in pharmaceutical and food/beverage industries. It is therefore of great interest to enhance extant understanding about this enzyme family. For this purpose, we used whole-genome shotgun metagenomics and genome binning and explored rumen of dairy cows, large intestine of horses, sediment of freshwater and topsoil of forest to identify novel prokaryotic FAEs and their producing microorganisms. A number of prokaryotic genomes were recovered from which five novel bacterial FAEs were predicted. The phylogenetic analyses showed that these putative FAEs are closely related to type A FAEs and might thus possess characteristics similar to type A FAEs. We nominate Candidatus Rhabdochlamydi genus as a novel FAE producing taxonomic unit.

Keywords: Biorefinery, genome binning, lignocellulosic biomass, Shotgun metagenomics, phylogeny

Received: 24 Jun 2019; Accepted: 04 Nov 2019.

Copyright: © 2019 Mogodiniyai Kasmaei and Sundh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Kamyar Mogodiniyai Kasmaei, Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala, Sweden,