Amelioration of Alcohol Induced Gastric Ulcers Through the Administration of Lactobacillus plantarum APSulloc 331261 Isolated From Green Tea

Gastric inflammation is an indication of gastric ulcers and possible other underlying gastric malignancies. Epidemiological studies have revealed that several Asian countries, including South Korea, suffer from a high incidence of gastric diseases derived from high levels of stress, alcoholic consumption, pyloric infection and usage of non-steroidal anti-inflammatory drugs (NSAIDs). Clinical treatments of gastric ulcers are generally limited to proton pump inhibitors that neutralize the stomach acid, and the application of antibiotics for Helicobacter pylori eradication, both of which are known to have a negative effect on the gut microbiota. The potential of probiotics for alleviating gastrointestinal diseases such as intestinal bowel syndrome and intestinal bowel disease receives increasing scientific interest. Probiotics may support the amelioration of disease-related symptoms through modulation of the gut microbiota without causing dysbiosis. In this study the potential of Lactobacillus plantarum APSulloc 331261 (GTB1TM), isolated from green tea, was investigated for alleviating gastric inflammation in an alcohol induced gastric ulcer murine model (positive control). Treatment with the test strain significantly influenced the expression of pro-inflammatory and anti-inflammatory biomarkers, interleukin 6 (IL6) and interleukin 10 (IL10), of which the former was down- and the latter up-regulated when the alcohol induced mice were treated with the test strain. This positive effect was also indicated by less severe gastric morphological changes and the histological score of the gastric tissues. A significant increase in the abundance of Akkermansia within the GTB1TM treated group compared to the positive control group also correlated with a decrease in the ratio of acetate over propionate. The increased levels of propionate in the GTB1TM group appear to result from the impact of the test strain on the microbial population and the resulting metabolic activities. Moreover, there was a significant increase in beta-diversity in the group that received GTB1TM over that of the alcohol induced control group.

Gastric inflammation is an indication of gastric ulcers and possible other underlying gastric malignancies. Epidemiological studies have revealed that several Asian countries, including South Korea, suffer from a high incidence of gastric diseases derived from high levels of stress, alcoholic consumption, pyloric infection and usage of non-steroidal anti-inflammatory drugs (NSAIDs). Clinical treatments of gastric ulcers are generally limited to proton pump inhibitors that neutralize the stomach acid, and the application of antibiotics for Helicobacter pylori eradication, both of which are known to have a negative effect on the gut microbiota. The potential of probiotics for alleviating gastrointestinal diseases such as intestinal bowel syndrome and intestinal bowel disease receives increasing scientific interest. Probiotics may support the amelioration of disease-related symptoms through modulation of the gut microbiota without causing dysbiosis. In this study the potential of Lactobacillus plantarum APSulloc 331261 (GTB1 TM ), isolated from green tea, was investigated for alleviating gastric inflammation in an alcohol induced gastric ulcer murine model (positive control). Treatment with the test strain significantly influenced the expression of pro-inflammatory and anti-inflammatory biomarkers, interleukin 6 (IL6) and interleukin 10 (IL10), of which the former was downand the latter up-regulated when the alcohol induced mice were treated with the test strain. This positive effect was also indicated by less severe gastric morphological changes and the histological score of the gastric tissues. A significant increase in the abundance of Akkermansia within the GTB1 TM treated group compared to the positive control group also correlated with a decrease in the ratio of acetate over propionate. The increased levels of propionate in the GTB1 TM group appear to result from the impact of the test strain on the microbial population and the resulting metabolic activities. Moreover, there was a significant increase in beta-diversity in the group that received GTB1 TM over that of the alcohol induced control group.

INTRODUCTION
Gastric ulcers, also known as peptic ulcers, develop within the epithelial lining of the stomach due to the reduction of the mucus layer by the enhanced secretion of acid and pepsin. Some of the main contributing factors to these erosion processes within the stomach are Helicobacter pylori infection, abuse of alcohol, smoking, or the prolonged use of non-steroidal antiinflammatory drugs (NSAIDs) (Toljamo et al., 2011). According to the systematic review on the global incidence of peptic ulcers by Azhari et al. (2018), South Korea has one of the highest annual incidences of perforated peptic ulcers in the 21 st century. The clinical approaches for treatment are still limited to proton pump inhibitors or the eradication of H. pylori, that includes antibiotic therapy. Most of the recommended drugs, such as the proton pump inhibitors prevent gastric ulcers through the suppression of acid production within the stomach, disrupting the protective pH barrier which promotes a higher risk of enteric infections caused by bacterial pathogens such as Clostridium difficile, Salmonella spp. and Campylobacter spp. (Imhann et al., 2017). Therefore, other alternative remedies are needed to counteract gastric ulcers by also protecting the gut from enteric infections.
Different strains of the lactic acid bacteria (LAB), and particularly some Lactobacillus spp., are representing some of the most commonly used probiotics, defined as "live microorganisms which, when administered in adequate amounts, confer a health benefit on the host" (Food and Agriculture Organization [FAO], and World Health Organization [WHO], 2002). Extensive research has been performed on the efficacy of various Lactobacillus strains on gastric ulcers (Dharmani et al., 2013;Khoder et al., 2016) either as single therapeutic agents or in combination with antibiotics (Boltin, 2016;Goderska et al., 2018). The majority of the investigated strains have been shown to inhibit or protect the gastric mucosal barrier through the up-regulation of prostaglandin E2 (Uchida and Kurakazu, 2004;Gotteland et al., 2006;Lam et al., 2007;Uchida et al., 2010), enhancement of mucus secretion (Gomi et al., 2013) or the regulation of inflammatory responses (Konturek et al., 2009;Şenol et al., 2014). Moreover, not only do these probiotics have prophylactic effects, but some of them also exert therapeutic effects through the enhancement of epithelial growth (Singh and Kaur, 2012), promotion of angiogenesis (Dharmani et al., 2013) and up-regulation effect on anti-inflammatory cytokines expression (Virchenko et al., 2015). Currently, approaches involving therapies either with "new" antimicrobials and/or probiotics are considered promising approaches for therapeutics or prophylactics of gastric ulcers (Goderska et al., 2018).
Several studies suggest that possible underlying mechanisms for the effect of probiotics on the host physiology may be related to their modulating role on the gut microbiota (Delzenne et al., 2011;Gerritsen et al., 2011;Gomes et al., 2014;Park et al., 2017;Ji et al., 2018). Yet, a deeper understanding of the impact of probiotic administration on ameliorating gastric ulcers is still lacking and needs further investigation and better understanding. Current knowledge is suggesting that butyrate, one of the three major short chain fatty acids (SCFAs) produced by the intestinal microbiota, has a protective effect against ethanol-induced gastric ulcer formation. Pre-treatment with butyrate down-regulated the pro-inflammatory cytokines IL1β, TNFα, and IL6 and enhanced the gastric wall mucus (Liu et al., 2016). Furthermore, a butyrate-producing Clostridium butyricum showed a similar effect as sodium butyrate in the alcohol induced gastric ulcer model and also alleviated the gastric mucosal damage and inflammation within the cold stress model and the pyloric induction model (Wang et al., 2015). A recent publication also demonstrated the protective effect of acetate against ethanol-induced acute gastric ulcers through the regulation of inflammation in the gastric mucosa (Liu et al., 2017). Even when the efficacy of probiotics, butyrate-producing species and short chain fatty acids on gastric erosion has been reported (Wang et al., 2015;Liu et al., 2017), data obtained in studies on the relationship between these administrations and the modulation of the gut microbiota in alcohol erosion models need further critical assessment in support of a better understanding on protective processes.
Probiotics are known to modulate the gut microbiota which may in turn regulate the production of the SCFAs within the gut. Therefore, in this study we explored the efficacy of Lb. plantarum APSulloc 331261 GTB1 TM by focusing on the production of SCFAs derived from the modulation of the gut microbiota in alcohol induced gastric ulcers.

Bacterial Culture Condition
Lactobacillus plantarum APSulloc 331261, isolated from green tea (Dolsongi tea field, Jeju island, South Korea) was provided by Amore Pacific (KCCM11179P), and its safety and beneficial properties were described previously (Arellano et al., 2019). Recently, the designation of this strain has been amended with its trademark (GTB1 TM ), and further reference to this strain (Lactobacillus plantarum APSulloc 331261 GTB1 TM ), will be as GTB1 TM . Lb. plantarum 299v is a commercial probiotic strain that has been investigated widely for its beneficial physiological functions, amongst others for improving irritable bowel syndrome (Ducrotté et al., 2012). The Lb. plantarum strains (GTB1 TM and 299v) were cultured at 37 • C for 18 h and prepared on a daily basis in MRS broth (Difco Laboratories Inc., Franklin Lakes, NJ, United States). Bacterial cell suspensions for experimental purposes were obtained by centrifugation of overnight cultures (12,000 × g for 1 min at 4 • C), the pellet was washed twice with sterile PBS (1x, pH 7.4, Lonza TM BioWhittaker TM , Walkersville, MD, United States) and re-suspended in PBS to match the concentration of 1 × 10 9 CFU per 200 µL of PBS before administration to the mice.

Murine Models
All procedures carried out in the animal study were approved by the Animal Ethical Committee of Handong Global University, South Korea (20160616-007). Four-week-old, specific pathogen free (SPF), Institute for Cancer Research (ICR) male mice obtained from Saeronbio Inc. (Gyeonggi-do, South Korea) were provided with sterilized water and normal chow diet (Purina, Chicago, IL, United States) at ad libitum and housed under 23 • C ± 1 • C and 55 ± 10% humidity in a 12 h light/dark cycle.

Alcohol Induced GU Murine Model
All the mice were adapted in the set environment for 1 week and then randomized into five groups (n = 8) as described: Group A -PBS (control group without ethanol induction); Group B -EtOH (control group with ethanol induction); Group C -AP (Lb. plantarum APSulloc 331261 GTB1 TM ); Group D -299v (probiotic control, Lb. plantarum 299v); Group E -OMPZ (most commonly used proton pump inhibitor drug control, Omeprazole).
The probiotics groups, receiving GTB1 TM and Lb. plantarum 299v, respectively, were pretreated with 1 × 10 9 CFU/day, for 7 days before ethanol induction, while the omeprazole group was fed with 13 mg/kg body weight/day of omeprazole according to the dose applied by Wang et al. (2015), for the same period. After the pre-treatments, the alcohol induced GU model was prepared and performed according to Liu et al. (2016) on 8 days and the mice were sacrificed 1 h after the induction. Part of the gastric tissue was fixated in 4% formaldehyde for histological assessment and the serum, cecum, liver, and stomach were collected and stored at −80 • C until further analysis.

Ethanol Erosion and H. pylori Infection Murine Model
Following the initial induction with ethanol and H. pylori infection, the groups received the probiotic strains (Lb. plantarum GTB1 TM and 299v) and the drug Omeprazole as control; sacrifice followed 2 weeks later. The 60% ethanol was fed orally to the pre-treatment and post-treatment groups 3 h prior to H. pylori infection by re-suspending 2 × 10 9 CFU/mL PBS and administering 0.5 mL to each animal according to Lee et al. (2006). All mice were sacrificed 2 weeks after the ethanol induction and H. pylori infection. Part of the gastric tissue was fixed in 4% formaldehyde for histological assessment and the serum, cecum, liver, and stomach were collected and stored at −80 • C until further analysis.

H&E Staining of Gastric Tissue
The paraffin blocks of the gastric tissues fixed in 4% formaldehyde were analyzed by the Korea Experimental Pathology Inc. (Gwangju-si, Gyeonggi-do, South Korea). These tissues were sectioned into 7 µm thick specimens and stained with hematoxylin and eosin (H&E). Observations were made under a light microscope to determine levels of pathological damage. The tissues were blindly scored according to gastric mucosal injury and neutrophil infiltration at a scale of 0-4 provided by Erben et al. (2014).

RNA Extraction and qRT-PCR Analysis
Gastric tissue was homogenized in 1 mL of TRIzol TM Reagent (Invitrogen TM , Thermo Fisher Scientific, San Diego, CA, United States) and RNA extracted according to the manufacture's protocol. SPECTROstar Nano (BMG Labtech, Offenburg, Germany) was used to evaluate the RNA concentration and purity. Two micrograms of complementary DNA were prepared using the GoScript TM Reverse Transcription System (Promega, Madison, WI, United States) and 5 min incubation with oligo-dT primer at 70 • C on the Verity 96-well thermal cycler (Applied Biosystems, Foster City, CA, United States). Specific primers for analyzing gastric cytokines (Supplementary Table S1), SYBR green Premix Ex Taq TM II (Takara, Shiga, Japan) and 20 ng of cDNA were mixed for each reaction using the Step-One Plus realtime PCR system (Applied Biosystems) for quantitative real-time PCR analysis according to Park et al. (2017).

Cecal Short Chain Fatty Acid (SCFA) Detection
The SCFAs, obtained from 40 mg of cecal content, were mixed with 150 µL of extraction buffer formulated according to Schwiertz et al. (2010) and lysed for 3 min in a Mini-Beadbeater-16 (BioSpec Products, Bartlesville, OK, United States). The mixture was incubated in a horizontal shaker at 25 • C for 1 h and then centrifuged at 16,000 × g and 25 • C for 5 min. The supernatant was carefully transferred into a 9 mm blue screw-capped clear glass vial with interlock insert (Agilent Technologies, Palo Alto, CA, United States). Volatile Free Acid Mix (Supelco, 46975-U, Sigma-Aldrich, St. Louis, MO, United States) was used to create a standard curve for the quantification of the cecal SCFAs that were detected using gas chromatography (Shimadzu GC2010, Shimadzu, Kyoto, Japan).

Cecum DNA Extraction and qRT-PCR Analysis
DNA was extracted by using the QIAmp DNA mini-kit (Qiagen, Valencia, CA, United States). 50 mg of the cecum were mixed with 0.3 g of 0.1 mm sterile zirconia beads with 700 µL of ASL Buffer (Qiagen) in a 2 mL bead beating tube and homogenized for 3 min in the Mini-Beadbeater-16. The following DNA extraction procedures were performed according to the QIAmp DNA Mini Kit manufacturer's instructions. Microbiota analysis of the supplementary model was performed by qRT-PCR and using the specific primers suggested by Bergström et al. (2012).

Cecum Library Preparation and Microbiota Analysis
Cecal genomic DNA was diluted with 10 mM Tris-HCl pH 8.5 buffer to 5 ng/µL prepared according to the Illumina 16S metagenomics sequencing library protocol. The 16S rRNA V3-V4 region was amplified using the following amplicon primers: 16S Amplicon PCR Forward Primer 5 TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG 3 and 16S Amplicon PCR Reverse Primer 5'GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C 3.
The amplified samples with linker primers were then barcoded using the dual indexing method involving the Nextera XT kit (Illumina, San Diego, CA, United States). The final products were normalized and pooled using PicoGreen, and the size of libraries were verified using the LabChip GX HT DNA High Sensitivity Kit (PerkinElmer, Waltham, MA, United States) and performed on an Illumina Miseq platform.
The barcode, linker, and primer sequences were then removed from the original sequencing reads and were replaced with sample names. The removed reads were then merged by their paired-ends using FLASH v 1.2.11. The merged reads containing two or more ambiguous nucleotides, those with a low-quality score (average score < 20), and reads shorter than 300 base pairs, were filtered out. Potential chimeric sequences were detected using the Bellerophon method. The pre-processed reads from each sample were used to calculate the number of operational taxonomic units (OTUs). The number of OTUs was determined by clustering the sequences from each sample using a 97% sequence identity cut-off and the taxonomic profiling and diversity of each group were analyzed and visualized using macQIIME software (v.1.9.1).

Statistics
All values are expressed as mean ± SD. Statistical differences were determined by one-way ANOVA using Fisher's Least Significant Difference (LSD). A p-value less than 0.05 was considered statistically significant.

Efficacy of Lb. plantarum APSulloc 331261 GTB1 TM Against Alcohol Induced Gastric Ulcer
The visual damage shown from the gross morphology of the gastric mucosa was significantly different in the GTB1 TM treated group (AP) after the induction with alcohol, and showed lower impairment of hemorrhaging gastric tissue ( Figure 1A). Furthermore, histological staining revealed less mucosal tissue damage while the pathological score in the AP fed group was significantly lower than that of the ethanol induced (EtOH) group (Figures 1B,C).

Expression of Inflammatory Cytokines in Alcohol Induced Gastric Tissue
There was no significant difference in the AP (GTB1 TM ) group compared to the EtOH group in the expression levels, neither of the anti-inflammatory cytokine interleukin 10 (IL10) nor the pro-inflammatory cytokine interleukin (IL6) (Figures 2A,B). However, the ratio of IL6/IL10 showed a significant difference with the EtOH group in both the non-treated PBS control and AP (GTB1 TM ) groups ( Figure 2C). Furthermore, in the study of the H. pylori infected mice after erosion of the gastric mucosa through alcohol induction, the inflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL1β) and interleukin 4 (IL4) were significantly down-regulated in the GTB1 TM treated mice when compared to the HP control (H. pylori infected) group and, with the exception of TNF-α, also the 299v group (Supplementary Figure S1).

Effects of Lb. plantarum APSulloc 331261 on SCFA Production
While no significant difference in the production of the SCFAs acetate, propionate and butyrate could be detected between the positive control (EtOH treated group) and the two LAB treated groups, OMPZ treatment resulted in a significant decrease in each of the three SCFAs (Figures 3A-C) and the total SCFAs ( Figure 3D). On the other hand, a significant decrease was recorded in the acetate/propionate ratio in the LAB treated and the omeprazole drug control (OMPZ) groups ( Figure 3E) and also in the acetate/butyrate ratio ( Figure 3F). Moreover, in the study on the H. pylori infected mice, all three SCFAs significantly increased only in the post-treatment GTB1 TM and OMPZ groups after erosion of the gastric mucosa through alcohol induction (Supplementary Figure S2).

Modulation of the Gut Microbiota Through the Administration of Lb. plantarum APSulloc 331261 GTB1 TM
The administration of GTB1 TM induced significant changes in the structure of the gut microbiota and the ratio among bacterial groups. Both the un-weighted and weighted beta diversity showed a significant shift in the microbial community when compared to the alcohol-induced (EtOH) control (Figures 4D,E). A clear distinction between the microbial communities is shown across the PC1 of the un-weighted principle coordinate analysis (PCoA) plot ( Figure 4D) and PC1 and PC2 of the weighted PCoA plot ( Figure 4E). Furthermore, the AP group showed significantly higher richness in alpha diversity (Chao 1 and observed OTUs) but lower evenness (Shannon diversity) compared to the EtOH control group (Figures 4A-C). At the genus level there was a significant increase of Akkermansia in the AP group compared to the EtOH group, while in the family Ruminococcaceae one genus was significantly decreased in the AP group (Figure 5). It is also noteworthy that, compared to all other groups, administration of GTB1 TM resulted in a significant increase in abundance of both Bifidobacterium spp. and Clostridium butyricum. More detailed information on modulation of the gut microbiota resulting from the different treatments is shown in Supplementary Figure S3.

DISCUSSION
Overconsumption of alcohol is known to impair the gastric mucosal barrier causing extensive hemorrhagic injuries, accumulate oxidative stress and increase inflammation through the production of cytokines such as IL1β, IL6, and TNF-α (Liu et al., 2017). The excessive use of alcohol not only causes organ dysfunction and health problems, but also disrupts the stability of the intestinal microbiome, resulting in what is generally known as dysbiosis (Engen et al., 2015). Some cases of microbial dysbiosis were associated with a decrease of Verrucomicrobiae Frontiers in Microbiology | www.frontiersin.org  and Bacteriodes and an increase in Gammaproteobacteria and Bacilli in alcoholics (Chen et al., 2011;Mutlu et al., 2012). In alcohol induced dysbiotic mice the Proteobacteria and Actinobacteria populations increased and were contrasted by a decrease in Bacteroidetes. On the other hand, the treatment of probiotics induced a shift in the three phyla in the opposite direction (Bull-Otterson et al., 2013). Furthermore, beneficial effects through the direct consumption of SCFAs, known as microbial by-products, have previously been proved effective in alcohol induced murine models (Liu et al., 2016(Liu et al., , 2017. In pilot studies several attempts have been made for the direct delivery of SCFAs as postbiotic, either via enemas or orally through a pH-dependent slow release mechanism. However, the results have been inconsistent thus far, and further research with a larger control group is required (Gill et al., 2018). It has been clearly established that short chain fatty acids are produced mainly in the colon as a result of microbial metabolism of nondigestible carbohydrates (Alexander et al., 2019). Lactobacillus and Bifidobacterium, the main genera used as probiotics, have been shown to produce acetate in vitro, however, they were not able to produce propionate and butyrate as primary metabolites. However, the administration of these common probiotics may stimulate the short chain fatty acid production of other colon bacteria through generating pyruvate and lactate from the dietary carbohydrates (LeBlanc et al., 2017). Moreover, probiotics, prebiotics and/or synbiotics can modulate the gut microbiota, and thereby inhibit pathogens and promote the growth of SCFA producing bacteria such as the propionate-producing genera Bacteriodes, Akkermansia and butyrate-producing genera such as Clostridium, Lachnospira, Roseburia and several others depending on the (probiotic) strain administered and the targeted disease (Louis and Flint, 2017).
It was shown previously that abundance of the genus Akkermansia is significantly decreased in the gut after the consumption of alcohol in an alcoholic hepatitis murine model (Lowe et al., 2017). Similar effects were confirmed in our experiments. The decrease in Akkermansia was significantly reversed by the administration of GTB1 TM compared to all the other control groups. Strains belonging to the genus Akkermansia, a member of the phylum Verrucomicrobia, are strictly anaerobic, oval-shaped, Gram-negative, mucin-degrading bacteria, and are highly abundant in the colon of a healthy person, comprising about 1-4% of the total population (Belzer and de Vos, 2012). Akkermansia is known to produce acetate (Derrien et al., 2004) and propionate through the fermentation of indigestible carbohydrates by the succinate pathway (Reichardt et al., 2014;Louis and Flint, 2017). The increase of Akkermansia therefore may explain the increase of propionate in the AP group (animals receiving GTB1 TM ); this was indicated by the significant decrease in the acetate/propionate ratio ( Figure 3E). There have been very few publications that suggest some corelation of Akkermansia with proinflammatory cytokines (Collado et al., 2012) and colorectal cancer (Weir et al., 2013). However, potential pathogenicity of Akkermansia has not been clearly defined. Furthermore, more studies prove that the decrease of Akkermansia was clearly correlated with diabetes, obesity and inflammatory bowel disease demonstrating a protective role of Akkermansia and also suggesting it as a promising probiotic (Zhang et al., 2019). The short chain fatty acids, acetate, propionate and butyrate are produced by bacterial fermentation of indigestible carbohydrates within the gut (Morrison and Preston, 2016). Liu et al. (2017) reported that although the administration of propionate did not show attenuation of gastric erosion in the alcohol induced model, it is widely known for its immunomodulatory effects in various gastrointestinal diseases, e.g. by inhibiting pro-inflammatory cytokines such as TNF-α, IL8 and IL6 in inflammatory bowel disease (Tedelind et al., 2007). Apart from the gut, the production of propionate influences the host metabolic health by decreasing lipid synthesis within the liver, regulating satiety through the stimulation of leptin and glucagon-like peptide-1, and lowering serum cholesterol levels (Hosseini et al., 2011;Park et al., 2018).
The GTB1 TM treated group showed significant differences in beta-diversity between the microbiota groups in both un-weighted and weighted PCoA plots (Figures 4D,E) demonstrating a different abundance and variety of the microbial communities between each group. Compared to the EtOH control group there was also a significantly higher richness shown through chao1 and the observed OTUs (observed operational taxonomic units) (Figures 4A,B) in the AP group, but a much lower evenness calculated by Shannon diversity. Also, in comparison with the EtOH control group, the richness in the non-treated PBS control group and the OMPZ group (Omeprazole treated) significantly increased ( Figure 4C). Loss of microbial diversity is used as one of the indicators of intestinal dysbiosis, especially in metabolic diseases caused by "Western Diet" which are typically characterized by a high amount of fat and sugar (Mosca et al., 2016). The AP group (receiving GTB1 TM ) showed an increase in richness. This suggests significant changes in particular microbial communities, and thereby an overall increase in alpha diversity. A similar change was detected in the OMPZ group. On the other hand, there was only a decrease in evenness of the GTB1 TM group, while for all the other groups significant changes could not be detected. Although there was a higher richness in the microbial genera of the AP group, the significantly lower evenness may have been mainly due to the increased ratio of Akkermansia within the community ( Figure 5B).
Furthermore, the beneficial effects of GTB1 TM administration were supported by histological analysis under the light microscope showing a morphologically significant lower gastric tissue erosion level an a significantly reduced pathological score. This result was in fact comparable to that of the OMPZ group, receiving omeprazole, the most commonly used drug for gastric ulcers (Figure 1). In both the GTB1 TM and OMPZ treated groups the production of the anti-inflammatory cytokine IL10 (Figure 2A), was increased, while only in the GTB1 TM group a lower production of the pro-inflammatory cytokine IL6 (Figure 2B) was detected. The IL6/IL10 ratio (Figure 2C) in the GTB1 TM group was significantly reduced compared to the EtOH group. An increase in the ratio of IL6/IL10 has been associated with gastric cancer prognosis (Madej-Michniewicz et al., 2015). Moreover, an increase in IL-6 in association with H. pylori pathogenicity and other peptic and duodenal ulcers has also been reported (Sugimoto et al., 2010;Cadamuro et al., 2014;Michalkiewicz et al., 2015).
In this study, we observed the preventive effects in ameliorating alcohol induced gastric erosion through the pretreatment with GTB1 TM . This pretreatment modulated the gut microbiota by increasing the diversity of the microbial community and the abundance of Akkermansia within the gut which in turn increased the ratio of acetate/propionate. The administration of GTB1 TM also increased the production of the anti-inflammatory cytokine IL10 concomitantly with a decrease FIGURE 5 | Taxonomic analysis of cecal microbiota from ethanol treated mice: (A) relative abundance of the genus level taxonomic composition, and (B) relative abundance of the genera Akkermansia and Ruminococcus. PBS: non-ethanol treated group, EtOH: ethanol treated control group, OMPZ: omemprazole treated group, 299v: Lactobacillus plantarum 299v, AP: Lactobacillus plantarum APSulloc GTB1 TM . Data was analyzed with one-way-ANOVA compared to EtOH *p < 0.05, **p < 0.01.
in IL6 production and an overall lowering in the inflammation of the gastric tissue. Our study showed that Lb. plantarum strain GTB1 TM displays potential probiotic functions through the modulation of the gut microbiota and amelioration of gastric erosion in an alcohol induced murine model.

DATA AVAILABILITY STATEMENT
The datasets generated for this study are available on request to the corresponding author.

ETHICS STATEMENT
The animal study was conducted at Handong Global University and was approved by the Animal Ethics Committee of Handong Global University, South Korea (20160616-007).

AUTHOR CONTRIBUTIONS
HP initiated and performed the experiments supported by EH. YJ and WH guided the initiation and planning of the work and the initial writing of the text together with HP. YJ, ST, and WH collaborated with HP in the interpretation of the data, and in the writing and final editing of the manuscript. YJ, WH, HP, and DC, participated in the experimental design. DC, JS, and WK worked on receiving the funding for this project.