AUTHOR=Hua Jingjing , Jia Xiangyin , Zhang Liang , Li Yanyan 

TITLE=The Characterization of Two-Component System PmrA/PmrB in Cronobacter sakazakii

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00903

DOI=10.3389/fmicb.2020.00903

ISSN=1664-302X

ABSTRACT=<p><italic>Cronobacter sakazakii</italic> is an opportunistic Gram-negative pathogen that could cause meningitis and necrotizing enterocolitis. Several Gram-negative bacteria use the PmrA/PmrB system to sense and adapt to environmental change by resistance to cationic antimicrobial peptides of host immune systems. The PmrA/PmrB two-component system regulates several genes to modify LPS structure in the bacterial outer membrane. The role of PmrA/PmrB of <italic>C. sakazakii</italic> has been studied within the current study. The results suggest that PmrA/PmrB plays a crucial role in modifying LPS structure, cationic antimicrobial peptide susceptibility, cell membrane permeability and hydrophobicity, and invading macrophage.</p>