TY - JOUR AU - Yang, Dong AU - Taylor, Zachary E. AU - Handy, Scott AU - Li, Shaoji AU - Liu, Jiawang AU - Stabenow, Jennifer AU - Zalduondo, Lillian AU - Jonsson, Colleen B. AU - Altman, Elliot AU - Kong, Ying PY - 2020 M3 - Original Research TI - Identification of Anti-tuberculosis Compounds From Aurone Analogs JO - Frontiers in Microbiology UR - https://www.frontiersin.org/articles/10.3389/fmicb.2020.01004 VL - 11 SN - 1664-302X N2 - The emergence of multidrug-resistant Mycobacterium tuberculosis (Mtb) strains has made tuberculosis (TB) control more difficult. Aurone derivatives have demonstrated promising anti-bacterial activities, but their effects against Mtb have not been thoroughly determined. In this study, we aimed to develop anti-TB compounds from aurone analogs. We used a fluorescent protein tdTomato labeled Mtb CDC1551 strain to screen 146 synthesized aurone derivatives for effective anti-TB compounds. The 9504, 9505, 9501, 9510, AA2A, and AA8 aurones inhibited the growth of Mtb with minimal inhibitory concentrations of 6.25, 12.5, 25, 25, 25, and 50 μM, respectively. We also examined cytotoxicities of the six leads against the human liver cell line HepG2, the primate kidney cell line Vero and human monocyte THP-1 derived macrophages. Three of the aurone leads (9504, 9501, and 9510) showed low cytotoxic effects on all three cell lines and high Mtb inhibitory efficacy (selectivity index > 10). Aurone 9504, 9501, AA2A, or AA8 significantly reduced the Mtb load in the lungs of infected mice after a 12-days treatment. We determined that the aurone leads inhibit Mtb chorismate synthase, an essential enzyme for aromatic acid synthesis. Our studies demonstrate the promise of synthetic aurones as novel anti-TB therapeutics. ER -