Methylobacterium ajmalii sp. nov., Isolated From the International Space Station

Four strains belonging to the family of Methylobacteriaceae were isolated from different locations on the International Space Station (ISS) across two consecutive flights. Of these, three were identified as Gram-negative, rod-shaped, catalase-positive, oxidase-positive, motile bacteria, designated as IF7SW-B2T, IIF1SW-B5, and IIF4SW-B5, whereas the fourth was identified as Methylorubrum rhodesianum. The sequence similarity of these three ISS strains, designated as IF7SW-B2T, IIF1SW-B5, and IIF4SW-B5, was <99.4% for 16S rRNA genes and <97.3% for gyrB gene, with the closest being Methylobacterium indicum SE2.11T. Furthermore, the multi-locus sequence analysis placed these three ISS strains in the same clade of M. indicum. The average nucleotide identity (ANI) values of these three ISS strains were <93% and digital DNA-DNA hybridization (dDDH) values were <46.4% with any described Methylobacterium species. Based on the ANI and dDDH analyses, these three ISS strains were considered as novel species belonging to the genus Methylobacterium. The three ISS strains showed 100% ANI similarity and dDDH values with each other, indicating that these three ISS strains, isolated during various flights and from different locations, belong to the same species. These three ISS strains were found to grow optimally at temperatures from 25 to 30°C, pH 6.0 to 8.0, and NaCl 0 to 1%. Phenotypically, these three ISS strains resemble M. aquaticum and M. terrae since they assimilate similar sugars as sole carbon substrate when compared to other Methylobacterium species. Fatty acid analysis showed that the major fatty acid produced by the ISS strains are C18:1−ω7c and C18:1−ω6c. The predominant quinone was ubiquinone 10, and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and an unidentified lipid. Therefore, based on genomic, phylogenetic, biochemical, and fatty acid analyses, strains IF7SW-B2T, IIF1SW-B5, and IIF4SW-B5, are assigned to a novel species within the genus Methylobacterium, and the name Methylobacterium ajmalii sp. nov. is proposed. The type strain is IF7SW-B2T (NRRL B-65601T and LMG 32165T).


INTRODUCTION
The genus Methylobacterium contains more species than any other genera within the family Methylobacteriaceae, order Rhizobiales, and class Alphaproteobacteria (Kelly et al., 2014). Methylobacterium species are Gram-negative, rodshaped bacteria. The genus was first proposed by Patt et al. (1976) with Methylobacterium organophilum as the type species. The genus Methylobacterium was first emended to include facultative methylotrophs that have the ability to grow on methane or methanol as the source of carbon and energy, in addition to sugars and organic acids (Patt et al., 1976). Another taxonomic study classified all other previously known pink-pigmented facultative methylotrophic bacteria under the genus Methylobacterium (Green and Bousfield, 1982). Thereafter, 11 species from the genus Methylobacterium were redefined into a new genus proposed as Methylorubrum, based on 16S rRNA gene sequence, multi-locus sequence analysis (MLSA), genomic, and phenotypic data (Green and Ardley, 2018).
The genus Methylobacterium consists of 45 recognized species, which are ubiquitously present in a wide variety of habitats including air, soil, freshwater, and sediments, and can exist either in free-form or associated with plant tissues (Gallego et al., 2005a,b;Kang et al., 2007;Veyisoglu et al., 2013;Kelly et al., 2014;Kwak et al., 2014;Chaudhry et al., 2016;Green and Ardley, 2018;Park et al., 2018). Methylobacterium species are involved in nitrogen fixation, phosphate solubilization, abiotic stress tolerance, plant growth promotion, and biocontrol activity against plant pathogens (Madhaiyan et al., 2006;Kumar M. et al., 2016;Parasuraman et al., 2019;Grossi et al., 2020;Krug et al., 2020). For instance, a novel Methylobacterium sp. 2A was observed to result in higher density of lateral roots in inoculated potato crops, even under salt stress conditions, compared with control plants that were not inoculated with the bacteria; it was also found to exhibit biocontrol activity against several plant pathogens (Grossi et al., 2020). Furthermore, genomic analysis of Methylobacterium sp. 2A revealed the presence of metabolic pathways involved in plant growth promotion, including the genes for producing an auxin, 3-indole acetic acid (Grossi et al., 2020).
In an ongoing Microbial Tracking experiment on the International Space Station (ISS), four strains belonging to the family Methylobacteriaceae were isolated (Checinska Sielaff et al., 2019). Some of the Methylobacterium species that are phylogenetically related to these ISS strains have been isolated from plant sources (Kang et al., 2007;Chaudhry et al., 2016), indicating that the ISS strains might also display properties related to plant growth promotion. The objectives of this study were to generate whole genome sequences (WGS) and define the phylogenetic novelty of the ISS Methylobacterium strains using polyphasic taxonomic analyses. The WGS generated and annotated in this study was used to predict biotechnologically useful genetic determinants.

Sample Collection and Isolation of Bacteria
Several surface samples (1 m 2 ) were collected from the ISS during Microbial Tracking-1 flight experiments from 2015 to 2016. Sample collection, processing, and isolation of cultivable microorganisms were published elsewhere (Checinska Sielaff et al., 2019). Briefly, the polyester wipes used to collect samples and particulates associated with the sampling devices were transported to Earth before being disassociated into sterile phosphate-buffered saline (pH 7.4) solution and plated onto R2A agar medium (Checinska et al., 2015;Checinska Sielaff et al., 2019). The microbial cultures that were grown at 25 • C for 7 days were picked from the R2A plates, purified, and stored for further analyses. Distinct colonies (n = 4) isolated from three different locations and from a high-efficiency particulate arrestance (HEPA) filter were characterized during this study. These colonies exhibited unique coloration and differential genomic phylogeny. The type strain IF7SW-B2 T was isolated during Flight 1 (March 2015) at Location #7, the Overhead-3 panel surface of the Materials Science Research Rack 1, which is used for basic materials research in the microgravity environment of the ISS. The second strain, IIF1SW-B5, was isolated during Flight 2 (May 2015) at Location #1, the Port panel of the Cupola. The Cupola is a small module devoted to the observation of operations outside the ISS, such as robotic activities, spacecraft approaches, and extravehicular activities. The third strain, IIF4SW-B5, was isolated during Flight 2 (May 2015) at Location #4, the surface of the dining table. Even though the main function of the table was for dining, crewmembers also used the table for experimental work. The fourth strain was I1-R3, isolated from the ISS HEPA filter that was returned aboard STS-134/ULF6 in May 2011 and archived as reported earlier (Checinska et al., 2015).

DNA Extraction and Whole Genome Sequencing Analysis
A biomass of approximately 1 µg wet weight was collected for DNA extraction from each strain after growing on R2A medium at 25 • C for 3 days. Total nucleic acid extraction was carried out using ZymoBIOMICS 96 MagBead DNA kit (lysis tubes) (Zymo Research, United States) after bead beating with a Bertin Precellys homogenizer. This was followed by library preparation using the Illumina Nextera Flex Protocol as per Illumina document number 1000000025416 v07. The initial amount of DNA for library preparation was quantified, and 5 to 12 cycles of polymerase chain reaction (PCR) amplification were carried out to normalize the output depending on the input DNA concentration. The amplified genomic DNA fragments were indexed and pooled in 384-plex configuration. Whole-genome shotgun sequencing was performed on a NovaSeq 6000 S4 flowcell PE 2 × 150 platform with a paired-end module. The data were filtered with NGS QC Toolkit v2.3 (Patel and Jain, 2012) for high-quality (HQ) vector-and adaptor-free reads for genome assembly (cutoff read length for HQ, 80%; cutoff quality score, 20). The number of filtered reads obtained were used for assembly with SPAdes 3.14.0 (Bankevich et al., 2012) genome assembler (kmer size-32 to 72 bases) using default parameters. The genome was annotated using the National Center for Biotechnology Information (NCBI) Prokaryotic Genome Annotation Pipeline 4.11 (Tatusova et al., 2016;Haft et al., 2018). In addition, functional annotation of genome and seed categories were assigned to the genome by implementing the Rapid Annotations using Subsystems Technology (RAST) tool (Aziz et al., 2008).
Genomes of all other strains used in this study were downloaded from NCBI, and the genomic relatedness of ISS strains was identified based on average nucleotide identity (ANI; FastANI) calculations (Jain et al., 2018) and digital DNA-DNA hybridization (dDDH) analysis (Meier-Kolthoff et al., 2013). FastANI was run on all the genomes using the default parameters: Mashmap identity cutoff I 0 = 80%, non-overlapping fragments of size l = 3Kb, and minimum count of reciprocal mappings τ = 50.

Phylogenetic Analysis
Phylogenetic analysis was carried out based on 16S rRNA gene sequencing, and MLSA using six housekeeping genes: ATP synthase F1 beta subunit (atpD), DNA strand exchange and recombination gene (recA), chaperone gene (dnaK), DNA-directed RNA polymerase subunit beta (rpoB), glutamine synthetase type I (glnI), and DNA gyrase subunit B (gyrB), for differentiating Methylobacterium species (Green and Ardley, 2018). The 16S rRNA gene sequences of type strains of all 45 Methylobacterium species were included in the phylogenetic analysis. In addition, representative species of genus Methylorubrum, Enterovirga, Microvirga, and Neomegalonema from family Methylobacteriaceae, Rhizobium from order Rhizobiales, Caulobacter from order Caulobacterales, in class Alphaproteobacteria were included. Pseudomonas aeruginosa was selected as the outgroup.
The 16S rRNA gene sequences of all strains were retrieved from NCBI except for the four ISS strains, which were recovered from their respective WGS. Phylogenetic analysis based on housekeeping genes and MLSA was carried out with type strains of 24 Methylobacterium species and representative species of other genera. All the gene sequences were retrieved from the genome sequences using RAST v2.0 1 (Aziz et al., 2008;Overbeek et al., 2014;Brettin et al., 2015). The individual gene sequences for all strains were aligned separately using ClustalW, and then the maximum likelihood tree was generated using MEGA 7.0.26 . For MLSA, six housekeeping gene sequences for each strain were concatenated manually and aligned using ClustalW, and then the maximum likelihood tree was generated using MEGA 7.0.26 .
The genome-based tree for the Methylobacterium species, including ISS strains and representative species of other genus with available WGS, was constructed using GToTree (Lee, 2019). This tool takes the complete/draft genomes as input and creates a phylogenomic tree based on the prespecified single-copy gene set using a hidden Markov model (HMM); the tool currently has 2,044 unique HMM set as identifiers to cover all three domains of microbial life.

Phenotypic Characterization of ISS Strains
Phenotypic characterization was performed according to standard protocols (Jones, 1981). Growth of the ISS strains at different temperatures (7,25,30,37, and 45 • C) was assessed after incubation on nutrient agar (Sigma, United States) for 7 days. Growth at different pH (4.0-10.0 at intervals of 1.0) was assessed after incubation in nutrient broth (Sigma, United States) at 30 • C for 7 days. The pH of the nutrient medium was adjusted using citrate/NaH 2 PO 4 buffer (pH 4.0-5.0), phosphate buffer (pH 6.0-8.0), and tris buffer (pH 9.0-10.0) (Kim et al., 2019). Salt tolerance was tested by streaking the strains on R2A supplemented with NaCl (0-10% at intervals of 1%) and incubating the plates at 30 • C for 7 days. Motility was assessed via the "hanging drop" method by observing the culture under a light microscope (Tindall et al., 2007). Catalase activity was tested by adding 3% hydrogen peroxide to culture grown on R2A at 30 • C for 7 days, and effervescence was monitored (Tindall et al., 2007). An oxidase test was carried out in a filter paper soaked with the substrate tetramethylp-phenylenediamine dihydrochloride, and coloration was documented (JurtshukJr., and McQuitty, 1976). All other physiological and biochemical tests were carried out using API 20 NE, API 50 CH, and API ZYM kits as per manufacturer's procedures (bioMérieux, France).

Chemotaxonomic Analysis
All strains grown in the R2A broth were harvested when growth of the cultures reached around 70% of the maximal optical density (exponential growth phase), and then the cultures were used for analyses of cellular fatty acids, polar lipids, and quinones, which were carried out as described previously (Ramaprasad et al., 2015). Briefly, for cellular fatty acids analysis, 40 mg of bacterial cell pellet from each strain was subjected to a series of four different reagents followed by saponification and methylation of fatty acids, thus enabling their cleavage from lipids. The fatty acid methyl esters (FAME) thus obtained were analyzed by a gas chromatograph equipped with Sherlock MIS software (Microbial ID; MIDI 6.0 version; Agilent: 6850) 2 . The peaks obtained were then labeled, and the equivalent chain length (ECL) values were computed by the Sherlock software.

RESULTS AND DISCUSSION
This study reports the isolation and identification of four strains belonging to the family Methylobacteriaceae, collected from different locations on the ISS. Three of the strains, referred to as IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5, were identified based on the traditional and genomic taxonomic approaches. The fourth strain, which was isolated from a HEPA filter and referred to as I1-R3, was identified based on genomic analyses only.

Phylogenetic Analysis of Novel ISS Strains
To confirm that three of the ISS strains (IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5) belong to a novel species, their phylogenetic affiliations were analyzed with other species belonging to the genus Methylobacterium. The sequence similarity of these three ISS strains with validly described Methylobacterium species was <99.4% for 16S rRNA gene (Supplementary Table 1   (Supplementary Figure 5) gene sequences. In addition, MLSA was carried out by concatenating the six housekeeping genes manually (Figure 3). In addition, a phylogenetic tree based on WGS was generated (Figure 4). The phylogenetic trees constructed based on all these genes, MLSA, and WGS showed that these three ISS strains (IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5) are clustered together and in the same clade with M. indicum SE2.11 T . The 16S rRNA gene-sequencing, housekeeping gene-based analyses, MLSA, and genome-based tree further supported the concept that these three ISS strains belong to the same species but are closely related to M. indicum.
In addition, the identity of the ISS strain I1-R3 was further confirmed to be M. rhodesianum based on its 16S rRNA gene (Figure 1) and gyrB (Figure 2) phylogenetic affiliation to the type strain M. rhodesianum DSM 5687 T .

Whole Genome Sequence-Based Phylogenetic Analysis
The genomes of the four isolated ISS strains were sequenced, with their draft genome assembled and annotated. The results are summarized in Table 1. The genome varied in size from 6.1 to 6.8 Mbp with GC content between 68 and 71%, similar to other members of the family Methylobacteriaceae. Due to higher sequence similarities of three ISS strains with M. indicum SE2.11 T (99.4% for 16S rRNA gene and 97.3% for gyrB gene), the draft genomes of three ISS strains were subjected to ANI and dDDH analysis with other species belonging to family Methylobacteriaceae ( Table 2). The ANI indices of three ISS strains (IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5) with M. indicum SE2.11 T were 92.7 to 93%, and dDDH values were 45.8 to 46.4%. The ANI and dDDH values obtained for three ISS strains with other Methylobacterium species were below the threshold of 95% ANI (Yoon et al., 2017) and 70% dDDH values (Auch et al., 2010), which were established for prokaryotic species delineation. This suggested that these three ISS strains are novel species of the genus Methylobacterium. These three ISS strains exhibited ANI and dDDH values around 99-100% with each other, indicating that they belong to the same species. The entire genomes of these three ISS strains, M. indicum SE2.11 T , and M. platani PMB02 T were aligned to detect their divergence and similarity using the MUMmer 3.0 system (Kurtz et al., 2004). As shown in Supplementary Figure 6, genomes of these three ISS strains aligned perfectly, while the closest genomes of M. indicum and M. platani exhibited divergence with the ISS type strain IF7SW-B2 T . Since these three ISS strains were isolated at different time periods and from various locations, their persistence in the ISS environment and ecological significance in the closed systems warrant further study.

Phenotypic Characterization of Novel ISS Strains
The minimal information about the ISS strain genome characteristics are given in Supplementary Table 2. The differential phenotypic characteristics of IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5 are listed in Table 3, in comparison with other related Methylobacterium species. Three strains belonging to Methylobacterium sp. nov. are reddish pink-pigmented, Gram-stain-negative, catalase-positive, oxidase-positive, motile, and rod-shaped. These strains grew well on nutrient agar and R2A. These three strains grew optimally at temperatures between 25 and 30 • C, were viable only at pH 6.0 to 8.0, and exhibited poor tolerance to salt (0 to 1%). Absence of growth was observed when grown at 7, 37, and 45 • C. These strains were positive for assimilation of L-arabinose, D-glucose, maltose, D-mannitol, D-mannose, malic acid, potassium gluconate, and trisodium citrate. These strains also exhibited esterase lipase and trypsin enzymatic activities. The complete results of phenotypic characteristics determined using API 20 NE, API ZYM, and API 50 CH are detailed in Supplementary Tables 3-5, respectively. The majority of the phenotypic characteristics of the ISS strains were similar to other Methylobacterium species. Phenotypically, these three ISS strains were different from the closest genomic relative M. indicum in assimilating glucose, malic acid, maltose, mannitol, potassium gluconate, and trisodium citrate. Furthermore, unlike M. indicum, these ISS strains did not exhibit growth at pH 5.0. In comparison to other Methylobacterium species, M. aquaticum and M. terrae exhibit similar carbon substrate utilization and enzyme production profiles. However, malic acid was assimilated by these ISS strains but not by M. aquaticum. Maltose was also utilized by these ISS strains but not by M. terrae cells.
The main phenotypic characteristics of the ISS strains IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5 were in accordance with the description of the genus Methylobacterium, with the most important being reddish pink pigmentation (Green and Bousfield, 1982). The optimum growth conditions (temperature, pH, salt tolerance) of the ISS Methylobacterium strains were similar to other members belonging to the genus Methylobacterium. Also, these three ISS strains shared the properties of exhibiting catalase activity and motility with other Methylobacterium species. However, the three novel ISS Methylobacterium strains differed from other members of the genus Methylobacterium in some of the phenotypic characteristics, as shown in Table 3. For instance, they exhibited properties like assimilation of certain sugars, which was absent in some of the Methylobacterium species. They also did not show cystine arylamidase activity as opposed to several related Methylobacterium species.

Chemotaxonomic Characterization of Novel ISS Strains
The FAME profiling of three ISS strains and other related Methylobacterium species are given in Table 4. The major fatty acids in these ISS strains were C 18:1 ω7c and/or C 18:1 ω6c (Sum  in Feature 8; 82 to 85%) with small amounts of C 18:0 3-OH, C 16:0 , C 17:0 , Sum in Feature 3, Sum in Feature 2, C 18:0 and C 12:0 , and traces of C 11:0 , C 13:0 , and C 14:0. The fatty acids, C 18:1 ω7c and/or C 18:1 ω6c, were observed to be dominant in these ISS strains, similar to other species. However, complete FAME profiles were not consistent among Methylobacterium species and some significant differences in the proportions of certain fatty acids were observed ( Table 4). The notable difference in the FAME profile was the lower abundance of C 18:1 ω7c in M. indicum (46%) when compared with these ISS strains (82 to 85%). These ISS strains contained Q-10 as the major respiratory isoprenoid quinone, which is common in members of the genus Methylobacterium. The polar lipids present in these three strains were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidyl choline (PC), phosphatidyl-ethanolamine (PE), and an unidentified lipid (Supplementary Figure 7). The total polar lipid profile of these ISS strains was consistent with their close relatives, predominated with phospholipids, DPG, PG, and PE. Furthermore, the chemotaxonomic data together with the results of the genomic and phylogenetic analysis support the affiliation of strains IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5 to the genus Methylobacterium.

Functional Characteristics of the Novel ISS Strain
The genome of the ISS strain IF7SW-B2 T , type strain, was annotated and analyzed to determine biotechnologically important genetic determinants. The whole genome and annotation analysis predicted a total of 6,531 genes in the assembled draft genome. Among these, 1,430 fell into various RAST categories, contributing to 2,067 predicted features described in Table 5. All the 1,430 feature and subsystems have been documented in Supplementary Data 1. A major fraction of the annotated genes was composed of amino acids and derivatives (408), carbohydrate metabolism (246), protein metabolism (198), genes associated with cofactors, vitamins, prosthetic groups, pigments metabolism (190), and respiration (151) ( Table 5). Genes responsible for motility and chemotaxis  Catalase , not determined. All strains exhibited 0-1% NaCl tolerance and were motile. *Growth was tested at 7, 25, 30, 37, and 45 • C and cells were grown optimally at 25 and 30 • C but no growth was observed at 7, 37, and 45 • C. Growth lower than 25 • C might be possible but not tested.
(95), metabolism of aromatic compounds (47), and stress response (72) were also observed. Based on the genome annotation, genes for nitrogen metabolism were predicted in the genome of the ISS strain IF7SW-B2 T . Most of the subsystem features aligned with the ammonia assimilation pathway (11 genes), which is a preferred nitrogen source for the bacteria (Leigh and Dodsworth, 2007). In addition, metabolic factors similar to high-affinity phosphate transporter and control of Pho regulon were also identified in the ISS strain IF7SW-B2 T (Wanner, 1993(Wanner, , 1996. Interestingly, a higher number of stress tolerance genes, especially the oxidative stress response factors, were observed in the ISS strain IF7SW-B2 T when compared with other novel species isolated from the ISS; Methylobacterium sp. IF7SW-B2 T exhibited 58 features, whereas 36 features were identified in Solibacillus kalamii (Seuylemezian et al., 2017) and 18 features were identified in Kalamiella piersonii (Singh et al., 2019). The results obtained agree with the previous reports that showed altered regulation of the stress response factors in microorganisms, in the presence of microgravity conditions (Orsini et al., 2017;Aunins et al., 2018). Further studies on the role of oxidative stress in species selection are warranted. The WGS assembly of these three ISS strains reported here will enable the comparative genomic characterization of ISS isolates with Earth counterparts in future studies. This will further aid in the identification of genetic determinants that might potentially be responsible for promoting plant growth under microgravity conditions and contribute to the development of self-sustainable plant crops for long-term space missions in future.

Genes Essential for Interaction With Plants in the ISS Strain
A thorough genomic analysis of the ISS strain IF7SW-B2 T revealed the presence of genes that have been involved in promoting plant growth. The isopentenyl tRNA transferase (miaA) essential for cytokinin production reported in M. aquaticum strain 22A (Tani et al., 2015) was also found in genome of the ISS strain IF7SW-B2 T with high similarity. The product of the miaA gene was reported to be responsible for isopentenylation of a specific adenine in some tRNAs and confirmed the secretion of zeatin originated from tRNA in M. extorquens (Koenig et al., 2002). Furthermore, multiple components of the cobalamin synthesis pathway, such as cobalamin biosynthesis protein BluB, L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), cobyric acid synthase (EC 6.3.5.10), nicotinatenucleotide-dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), adenosylcobinamide-phosphate synthase (EC    (Lee and Jeon, 2018). -: Not detected, tr: trace amount (<1%). *Summed features represent groups of two or three fatty acids that cannot be separated using the MIDI system. Summed feature 2 (iso-C16:1 I and/or C14:0 3-OH), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), Sum In Feature 5 comprises of C18:0-ω6,9c and ante-C18:0 and summed feature 8 (C18:1 ω7c and/or C18:1ω6c).  Figure 8). Supporting this prediction, previous study also reported that Methylobacterium strains harbor genes involved in the production of a variety of vitamins, such as cobalamin, biotin, thiamin, and riboflavin, indicating the potential of methylobacteria promoting algal growth (Krug et al., 2020). In addition, genes associated with siderophore production, i.e., ferric siderophore transport system, biopolymer transport protein ExbB, and multiple flagellar proteins, were identified in genome of the ISS strain IF7SW-B2 T and are listed in the Supplementary Figure 8. Genes involved in iron acquisition and metabolism in which microalgae benefit from bacterial siderophores have been reported previously in Methylobacterium spp. (Krug et al., 2020). In the "carbon for iron mutualism" concept, algae assimilated iron complexed in bacterial siderophores and in return provided essential dissolved organic matter for the bacteria (Amin et al., 2015). Similar studies are warranted to confirm the plant-growth promoting activities in the IF7SW-B2 T ISS strain.
In summary, the phylogenetic and genetic distinctiveness and differential phenotypic properties were sufficient to categorize these three ISS strains as members of a species distinct from other recognized Methylobacterium species. Therefore, on the basis of the data presented, strains IF7SW-B2 T , IIF1SW-B5, and IIF4SW-B5 represent a novel species of the genus Methylobacterium, for which the name Methylobacterium ajmalii sp. nov. is proposed. The type strain is IF7SW-B2 T (NRRL B-65601 T and LMG 32165 T ).
The type strain IF7SW-B2 T is isolated from the International Space Station.

DATA AVAILABILITY STATEMENT
The 16S rRNA gene sequences of Methylobacterium sp. IIF1SW-B5, and Methylobacterium sp. IIF4SW-B5 are submitted under accession numbers KY218843 and KY218865, respectively. The WGS and the raw data deposited under BioProject accession number PRJNA634337. The WGS accession numbers are mentioned in Table 1. The WGS was also deposited in GeneLab under GeneLab dataset (GLDS-300; https://genelabdata.ndc.nasa.gov/genelab/accession/GLDS339 300). The version described in this paper is the first version.

AUTHOR CONTRIBUTIONS
KV and NKS conceived and designed the experiments. SB, VE, and NKS performed the experiments. NKS analyzed the genomic data inclusive of de novo assemblies and verification, scaffold quality assessment, and annotation and generation of the whole genome and protein level alignment for positional description of organism in the tree of life. SB independently verified the genome assembly, generated alignments for all gene trees in the manuscript, and manually curated the tree images. KV and NKS isolated the type strain, and NKS carried out the phenotypic assays and biochemical characterization. KV compiled the contribution of write-ups from all authors associated with phenotype, NKS generated genotype and tables, and SB generated phylogenetic trees and figures. VE conducted the SB generated chemotaxonomic analysis. All authors read and approved the final manuscript. CEM generated the genomic library and sequenced the genomes of all strains. CCCW and ARP reviewed the manuscript.