TY - JOUR AU - Kwon, Sun-Jung AU - Bodaghi, Sohrab AU - Dang, Tyler AU - Gadhave, Kiran R. AU - Ho, Thien AU - Osman, Fatima AU - Al Rwahnih, Maher AU - Tzanetakis, Ioannis E. AU - Simon, Anne E. AU - Vidalakis, Georgios PY - 2021 M3 - Original Research TI - Complete Nucleotide Sequence, Genome Organization, and Comparative Genomic Analyses of Citrus Yellow-Vein Associated Virus (CYVaV) JO - Frontiers in Microbiology UR - https://www.frontiersin.org/articles/10.3389/fmicb.2021.683130 VL - 12 SN - 1664-302X N2 - Citrus yellow-vein disease (CYVD) was first reported in California in 1957. We now report that CYVD is associated with a virus-like agent, provisionally named citrus yellow-vein associated virus (CYVaV). The CYVaV RNA genome has 2,692 nucleotides and codes for two discernable open reading frames (ORFs). ORF1 encodes a protein of 190 amino acid (aa) whereas ORF2 is presumably generated by a −1 ribosomal frameshifting event just upstream of the ORF1 termination signal. The frameshift product (717 aa) encodes the RNA-dependent RNA polymerase (RdRp). Phylogenetic analyses suggest that CYVaV is closely related to unclassified virus-like RNAs in the family Tombusviridae. Bio-indexing and RNA-seq experiments indicate that CYVaV can induce yellow vein symptoms independently of known citrus viruses or viroids. ER -