Mycobacterium leprae Infection in Ticks and Tick-Derived Cells

Leprosy is a zoonosis in the southern United States involving humans and wild armadillos. The majority of patients presenting with zoonotic strains of Mycobacterium leprae note extensive outdoor activity but only rarely report any history of direct contact with wild armadillos. Whether M. leprae is transmitted to new vertebrate hosts through the environment independently or with the aid of other organisms, e.g., arthropod vectors, is a fundamental question in leprosy transmission. The objectives of this study were to assess the potential for ticks to transmit M. leprae and to test if viable M. leprae can be maintained in tick-derived cells. To evaluate tick transmission, nymphal Amblyomma maculatum ticks were injected with isolated M. leprae. Infection and transmission were assessed by qPCR. Ticks infected as nymphs harbored M. leprae through vertical transmission events (nymph to adult and adult to progeny); and, horizontal transmission of M. leprae to a vertebrate host was observed. Mycobacterium leprae DNA was detected in multiple tick life cycle stages. Likewise, freshly isolated M. leprae (Thai-53) was used to infect a tick-derived cell line, and enumeration and bacterial viability were assessed at individual time points for up to 49 days. Evaluations of the viability of long-term cultured M. leprae (Thai-53 and Br4923) were also assessed in a mouse model. Tick-derived cells were able to maintain viable M. leprae over the 49-day course of infection and M. leprae remained infectious within tick cells for at least 300 days. The results of this study suggest that ticks themselves might serve as a vector for the transmission of M. leprae and that tick cells are suitable for maintenance of viable M. leprae for an extended period of time.


INTRODUCTION
Hansen's Disease, more commonly known as leprosy, is caused by an obligate intracellular bacillus, Mycobacterium leprae. Presenting as a granulomatous infection that involves the skin and peripheral nerves, leprosy can deform and disable individuals if not properly and promptly treated (Walker and Lockwood, 2006). Current disease control strategies depend on the early diagnosis and treatment of new leprosy cases. However, poor diagnostic criteria for leprosy and the stigma that surrounds it act as barriers to both diagnosis and treatment (Predaswat, 1992;World Health Organization [WHO], 2019). A lack of understanding of how leprosy is spread also confounds efforts to eliminate the disease, as transmission is thought to occur mainly through exposure of susceptible individuals to bacilli shed from untreated or asymptomatic cases, though as yet the exact mechanisms are still unclear (Davey, 1974;Scollard, 2005). Environmental contamination and zoonotic transmission from non-human reservoirs also have been implicated (Blake et al., 1987;Desikan and Sreevatsa, 1995;Truman and Fine, 2010;Truman et al., 2011;Sharma et al., 2015;Avanzi et al., 2016;Mohanty et al., 2016;Tio-Coma et al., 2019).
Though the majority of new cases of leprosy in the United States can be associated with international travel to regions where the disease is more prevalent, 38% of the new cases identified in 2014 were considered autochthonous (HHS, 2015) and many of those appear to have evolved through zoonotic transmission of leprosy bacilli. Nine-banded armadillos (Dasypus novemcinctus) in the southern United States, as well as in Central and South America, harbor a natural infection with M. leprae that was likely introduced to the animals sometime after colonization of the New World. Large numbers of infected armadillos were known to exist in the southern United States well before the animals were ever used in leprosy research, and recent studies confirm that armadillos are a source of infection for many leprosy cases in the United States (Truman et al., 1986;Sharma et al., 2015). While direct contact with the blood or tissues of infected armadillos is recognized as an important risk factor for zoonotic transmission of M. leprae from armadillos, about half the cases in the United States infected with zoonotic M. leprae straintypes are unable to recall ever having any direct contact with the animals, and other mechanisms also must be involved (Truman et al., 2011).
Investigators have explored the potential role of arthropods in leprosy transmission for more than 80 years. Though conclusive evidence has not been generated, their role has never been fully discounted (Fine, 1982). The potential for arthropod-derived cells to support viable bacteria in vitro is supported by the identification of an organism belonging to the Mycobacterium chelonae complex being recently isolated while generating a primary cell line from field-caught ticks (Palomar et al., 2019). Likewise, studies have examined the potential for ticks to acquire and transmit M. leprae (Souza-Araujo, 1941;Ferreira et al., 2018). In the United States, Amblyomma species of ticks transmit a wide variety of infectious organisms and feed on both armadillos and humans (Bechara et al., 2002;Sumner et al., 2007;Harris et al., 2017;Mertins et al., 2017;Suwanbongkot et al., 2019). Specifically, Amblyomma maculatum has a distribution which largely overlaps with that of the nine-banded armadillo (Teel et al., 2010;Taulman and Robbins, 2014). The tick's geographical range is expanding, and includes Florida, Louisiana and Texas, three of the top five states reporting new cases of leprosy (Taulman and Robbins, 2014;HHS, 2015;Sonenshine, 2018). Knowing that A. maculatum is an important tick vector of intracellular bacterial pathogens transmissible to vertebrate hosts (Paddock et al., 2004;Ferrari et al., 2012;Grasperge et al., 2014;Banajee et al., 2015;Paddock and Goddard, 2015), this tick as a potential vector for M. leprae transmission was examined. In the absence of an available A. maculatum-derived cell line, the ISE6, Ixodes scapularis-derived cell line was chosen in this study based on the functional use of the cell line for cultivation of other obligate intracellular bacteria (Kurtti et al., 1996;Pornwiroon et al., 2006;Sunyakumthorn et al., 2008). Thus, in the current study we investigated the potential for A. maculatum ticks to harbor M. leprae and assessed the ISE6 cell line for its potential to propagate bacilli.

Ticks
A colony of Mycobacterium-free Amblyomma maculatum ticks was maintained at the Louisiana State University School of Veterinary Medicine as described previously (Grasperge et al., 2014). Briefly, immature and adult tick groups were fed separately via encapsulation on BALB/c mice and Sprague-Dawley rats, respectively. Ticks off host were kept in a 27 • C incubator at 90% relative humidity.

Bacteria
Mycobacterium leprae strains; Thai-53 and Br4923 were used in these studies. Both M. leprae strains were maintained and isolated from nude mice footpads by the National Hansen's Disease Program, Laboratory Research Branch (Truman et al., 2004;Montoya et al., 2019).

Tick Infection Bioassays, Sample Collection, and Mycobacterium leprae Detection
Microinjection was employed to expose nymphal and adult ticks as previous described (Kariu et al., 2011), with minor modification for M. leprae. Briefly, Drummond 0.4 mm diameter 75 mm length glass capillaries were pulled and fitted with a 0.50 ml Hammond syringe to aspirate a 1:1 dilution consisting of 0.1% Rhodamine B and 1 × 10 9 bacteria/ml in the M. leprae suspension. For ticks injected as nymphs (IAN), a capillary needle was inserted into the anal pore and the suspension (M. leprae solution was approximately 5 × 10 3 M. leprae/nymph) injected until the nymph was slightly distended. Immediate mortalities were discarded as they occurred. Injected nymphs were stored in an incubator for 24 h and then assessed for viability and fluorescent detection of Rhodamine B, as described (Harris et al., 2017). Surviving ticks that were positive by fluorescence were allowed to feed to repletion and molt into adults. A portion of these IAN adults were immediately used whole for DNA extraction. Another subsample of IAN adult female ticks was fed with unexposed male ticks for 1, 3, or 6 days before whole ticks were processed for DNA extraction. Individual IAN adult females were allowed to feed until repletion, oviposit, and three pools of either F 1 larvae (n = 30 larvae/pool) or F 1 nymphs (n = 5 nymphs/pool) from individual ticks were placed in TRIzol until DNA extraction, while the remaining F 1 progeny were allowed to molt to the adult stage. F 1 adult female ticks were fed with unexposed male ticks for 1, 3, or 6 days before whole ticks were processed for DNA extraction.
Adult tick injections were carried out as described for the nymphs, except ticks were not "filled" until distended (M. leprae solution was approximately 5-10 × 10 3 M. leprae/adult tick) and injections were direct into the hemocoel through the caudal margin of the tick, rather than through the anal pore. The injected as adult (IAA) ticks were fed on vertebrate hosts and removed shortly before full engorgement or following natural detachment for saliva collection and tissue recovery. Briefly, ticks were removed from the host and sequentially washed in 70% ethanol and deionized water. Salivation was induced through dorsal application of a 3% pilocarpine in EtOH and collected saliva was pooled into microcentrifuge tubes for subsequent DNA extraction. Subsequently, tick tissues (salivary gland and midgut) were recovered, snap frozen in liquid nitrogen and crushed with a sterile plastic pestle before the addition of TRIzol Reagent (ThermoScientific, CA, United States). and storage at −80 • C until DNA extraction previously described (Patton et al., 2012;Suwanbongkot et al., 2019). Samples of vertebrate host skin were collected from the tick feeding site, as well as skin outside the feeding capsule. All skin samples were immediately processed for DNA extraction (Patton et al., 2012;Suwanbongkot et al., 2019). DNA extracted from tick and vertebrate host samples was evaluated for the presence of M. leprae. Mycobacterium leprae-specific repetitive element (RLEP) qPCR was used to quantify M. leprae as previously described (Truman et al., 2008). Primer sequences were listed in Table 1.

Tick Cell Culture and Mycobacterium leprae Infection
The ISE6 tick-derived cell line (Ixodes scapularis-derived), provided by T. Kurtti (University of Minnesota), was maintained with Leibovitz's L-15B medium (Gibco-BRL, NY, United States) supplemented with 10% tryptose phosphate broth (pH 6.8-7.0, Sigma, MD, United States), 10% fetal bovine serum (HyClone, UT, United States), and maintained at 32 • C in a 5% CO 2 humidified incubator. For experiments, ISE6 cells at cell density 7 × 10 6 cells/flask were infected with 1 × 10 8 M. leprae/T25 flask (MOI of 15) and maintained in a 30 • C incubator. The presence of M. leprae in ISE6 cells was routinely assessed by comparing infected and uninfected cells. Briefly, cells cytocentrifuged onto a slide and stained with Fite's acid fast staining for 20 min at room temperature (Job and Chacko, 1986). Slides were rinsed with water and 1% acid alcohol was used to decolorize the slides for 1 min or until they were a faint pink. Slides were rinsed again with water, counterstained with methylene blue for 2 min prior to a final rinse, and then mounted in Permount TM (Fisher Scientific). Images were captured using Olympus BX53 microscope.

Enumeration and Viability Assays for
Mycobacterium leprae in Tick Cell Culture ISE6 cells were seeded in 5 ml in a T25 flask at a density of 7 × 10 6 cells/flask and incubated for 24 h prior to M. leprae (Thai-53; 1 × 10 8 ) infection. Cells and supernatants were collected at 21, 35, and 49 days post infection (dpi). Briefly, cell pellets and supernatant were collected at each time point, homogenized in a TissueLyser II (QIAGEN, Germany) using a speed setting of 6.5 m/s for 45 s, and nucleic acid was extracted using TRIzol Reagent. Extracted DNA and RNA were stored at −80 • C until qPCR was performed. The RLEP qPCR assay previously described for M. leprae was used for enumeration (Truman et al., 2008). Normalized esxA gene expression was used to determine M. leprae viability. Briefly, extracted RNA following DNAse (Fisher Thermo Scientific) treatment and normalization using RLEP counts, was converted to cDNA (cDNA Synthesis Kit-Advantage R RT for PCR kit (Clonetech, CA, United States) and esxA gene expression determined by qPCR as previously described (Davis et al., 2013) and sequences of primers are listed in Table 1. Viability in this assay is verified using a standard curve, mock controls in which no reverse transcriptase was used, and positive mouse foot pad controls infected with live M. leprae. Two biological replicates for infection assays were performed.

Transmission Electron Microscopy Imaging
For TEM analysis, ISE6 cells were seeded on plastic cover slips (Thermanox R ; TMX Coverslips) placed into a 24-well plate at a density of 5 × 10 5 cells/well. After 24 h, ISE6 cells were infected with freshly isolated M. leprae suspension (5 × 10 7 M. leprae/well). Media was removed at 21, 35, and 49 dpi and cover slips were washed with PBS. Fixative solution (3% Glutaraldehyde in 0.1 M CAC buffer, pH 7.4) was added to each well for 30 min at room temperature. Cover slips were then washed three times with 0.1 M CAC buffer with sucrose, pH 7.4 and post fixed with 1% Osmium tetroxide in 0.1 M CAC buffer at room temperature for 1 h. Araldite-Epon 812 (EP) was prepared with 60 g of Aradite (60 g of Polybed 812, 123 g DDSA). Samples were dehydrated with graded series of EtOH (30, 50, 70, 80, 90, and 100%) and embedded gradually with different ratios of EP to EtOH (1:3, 2:2, 3:1, EP only and EP + DMP-30). Sections were then stained with 4% uranyl acetate for 2 min and lead citrate for 1 min. Coverslips and all chemicals were purchased from Electron Microscopy Sciences (Hatfield, PA, United States). Images were captured using TEM: JEOL JEM-1011 microscope (JEOL, Inc., MA).

Evaluation of Mycobacterium leprae
Replication and Viability in ISE6 Cells During Extended Culture M. leprae Thai-53 and Br4923 were maintained in ISE6 cells in 30 • C incubator and subcultured (1:2) every 8 weeks into uninfected tick cells to avoid overgrowth of ISE6 cells.  Davis et al., 2013 At approximately 6 month-intervals, M. leprae were semipurified via needle lysis of host cells with low-and high-speed centrifugation (Simser et al., 2001;Sunyakumthorn et al., 2008) and inoculated into T75 uninfected tick cells at cell density of 1.0-1.8 × 10 8 cells/flask. The cell pellets were collected at 716, 729 dpi for Thai-53 and Br4923 strains, respectively. Samples were processed for imaging as previously detailed.

Mouse Footpad Infection With Mycobacterium leprae Maintained in ISE6 Cells
Both M. leprae strains Thai-53 and Br4923 maintained in ISE6 cells for 294 and 309 days, respectively, were purified and inoculated in athymic (nu/nu) mice (Envigo, CA) footpads, to assess their in vivo infectivity and growth as previously described (Truman and Krahenbuhl, 2001). Briefly, both hind footpads were inoculated with media alone (control) or either strain of M. leprae. The footpads were harvested at 6 months post-inoculation and immediately processed for DNA and RNA isolation to determine M. leprae numbers (RLEP) and viability (normalized esxA expression).

Statistical Analysis
Numerical data were analyzed using non-parametric Mann-Whitney test. All statistical analyses were performed with GraphPad Prism 9.0 software.

Tick Infection With Mycobacterium leprae and Subsequent Transmission
Using the RLEP qPCR, the presence or absence of M. leprae in tick samples was assessed after microinjection, during the tick life cycle, and in the vertebrate host skin where the ticks had attached. Of the 29 IAN ticks that molted to the adult stage assessed for M. leprae DNA, 71% of the unfed adults were positive ( Table 2). Of the ticks that were allowed to feed as adults, 40 and 33% of those that fed for 1 and 6 days, respectively, were positive for M. leprae DNA. None of the ticks assessed at 3 days of feeding (n = 4) were determined to be infected with M. leprae. Additionally, M. leprae DNA was detected in the F 1 larvae and F 1 nymph pools, but not in the F 1 adult ticks. For IAN ticks feeding on mice, 50% (n = 18) of the skin samples at the tick feeding site were positive for M. leprae. A mean (±SD) of 390 (±804) and 630 (±1040) M. leprae were detected inside and outside the capsule, respectively. The data suggest that while M. leprae can be maintained through transstadial (from nymph to adult) and transovarial (adult female to progeny) transmission events, sustained infection through the entire F 1 life cycle was not observed. The microinjected adult ticks (IAA; n = 6) had detectable levels of M. leprae in the midgut tissue (∼66%) and salivary gland samples (∼33%), with one tick positive in both tissues. Pooled saliva collected from two adult ticks exposed to M. leprae was also positive by PCR.

Mycobacterium leprae Can Infect and Persist in Tick-Derived Cells
For successful transmission of M. leprae, an obligate intracellular pathogen, the vector must be able to internalize and then, either support growth or maintain viability of the intracellular organisms for a period sufficient to find and transmit to the host. Tick-derived ISE6 cell lines were infected with M. leprae (Thai-53) for 21, 35, and 49 days. No significant changes in the M. leprae numbers, as enumerated by M. leprae specific RLEP qPCR, were observed in tick cell culture at the time points assessed (Figure 1). Both acid fast staining (Figure 2) and TEM (Figure 3) confirmed presence of intracellular bacilli at all these time points. There were no apparent differences in cell morphology or growth characteristics between infected and uninfected ISE6 cells at any of these time points.

Mycobacterium leprae Can Survive in Tick-Derived Cells
As ISE6 cells were able to maintain M. leprae numbers, bacterial viability was assessed by determining the normalized expression of esxA gene. Cells and supernatant were collected separately to quantify intracellular and extracellular M. leprae, respectively. At 49 days post-inoculation, >90% of the bacteria were intracellular (Figure 4A). Both infected-ISE6 cells and culture supernatant had viable M. leprae (Figure 4B). Together these data show that tick derived ISE6 cells do not support propagation but can maintain intracellular M. leprae number and viability for at least 49 days.  Total  17  5  4  3  3  3  3  2  2   RLEP positive  12  2  0  1  3  3  0  0  0 Vertical transmission, from one life cycle stage to the next, was observed for ticks infected as nymphs (IAN), with persistent infection waning by the adult stage of the subsequent generation. The numbers in parentheses are the number of larvae or nymphs per pool. Mycobacterium leprae DNA quantification was accessed by the RLEP qPCR. Two biological replicates were performed. Statistically significant differences (p < 0.05) were analyzed by Mann-Whitney test when comparing with initial suspension.

Prolonged Maintenance of Viable Mycobacterium leprae in Tick-Derived Cells
In addition to the limited time-course infection assay for M. leprae in tick cells, bacteria were maintained in cells by regular 1:2 splitting and providing new cells every 8 weeks. Both M. leprae strains, Thai-53 and Br4923, were maintained in the laboratory culture for 716 and 729 days, respectively. Mycobacterium leprae were observed under light microscopy by acid-fast staining ( Figure 5A) and by TEM (Figure 5B), confirming the presence of bacilli in the tick cells after 700 days.

Tick Cell Cultured Mycobacterium leprae Is Infectious for a Vertebrate Host
Both M. leprae strains Thai-53 and Br4923 were maintained in tick-derived cells for a prolonged period (around 300 days) and then inoculated in athymic nu/nu foot pads. MFPs were harvested 6-month post-inoculation to determine M. leprae growth and viability. With no significant differences between strains, both Thai-53 and Br4923 showed appreciable growth ( Figure 6A) and were highly viable ( Figure 6B) in MFPs. This confirms that tickderived ISE6 cells were able to maintain intracellular M. leprae viability and subsequent infectivity to vertebrate host even after 300 days of in vitro culture.

DISCUSSION
Ticks are effective vectors for transmitting a variety of diseasecausing agents, including numerous obligate intracellular bacteria (Laukaitis and Macaluso, 2021). These data indicate that Amblyomma ticks infected with M. leprae can successfully transmit the organisms both vertically and horizontally, making it possible for them to transfer the organisms to other hosts in subsequent blood meals. Once infected, ticks retain these bacilli through subsequent life-cycle stages and they can pass the organisms along to their progeny. Mycobacterium leprae also may have a special affinity for tick cells, as evidenced by the extraordinarily long survival time (≥ 300 days) of the bacilli with in vitro cultures of tick cells. In combination, ticks can be effective vehicles aiding the spread of leprosy bacilli between human and zoonotic hosts and their role in perpetuating leprosy merits additional investigation. Investigating the role ticks may have in the transmission of leprosy is not without precedent. In the 1940s, acid-fast bacilli were found within the gut tissue of Amblyomma spp. ticks fed on a leprosy patients' skin lesion, suggesting the ability to acquire bacilli (Souza-Araujo, 1941). More recently, Amblyomma sculptum ticks were determined capable of sustaining M. leprae infection (Ferreira et al., 2018). In the United States, Amblyomma spp. ticks were recovered from 50% of armadillos trapped, with an average 6 ticks per host (Mertins et al., 2017). Importantly, A. maculatum, an aggressive human biting tick has expanding geographical range that overlaps with that of the nine-banded armadillo (Taulman and Robbins, 2014;Sonenshine, 2018).
Employing a microinjection technique to expose ticks to M. leprae, nymphs were able to maintain infection and transstadially transmit to the adult stage. Likewise, adult ticks could transovarially transmit M. leprae to larvae, which remained infected into the nymphal stage. It has been demonstrated that bacterial loads in ticks can be coupled with the feeding activity (Suwanbongkot et al., 2019); however, feeding F 1 adult ticks that were infected as nymphs did not result in detectable levels of M. leprae. While it is possible that bacilli were shed in the feces of ticks during the feeding process, the presence and viability of M. leprae in tick feces requires further examination. Similar infection kinetics were observed for spotted fever group Rickettsia in A. maculatum ticks (Harris et al., 2017), it is possible that vertebrate amplification hosts may be needed for sustained pathogen infection in tick populations.
Delivery of M. leprae via an infected tick may help identify a route of horizontal transmission between zoonotic and human hosts. Of the ticks exposed to M. leprae as adults and allowed to take a blood meal, RLEP was detected in midguts and salivary glands after feeding, suggesting organs essential for transmission can become infected. Additionally, pooled saliva collected from these ticks were also positive for RLEP, confirming the potential for horizontal transmission. The detection of M. leprae in the skin of vertebrate hosts fed on by recently exposed nymphal FIGURE 3 | TEM imaging on M. leprae Thai-53 strain infected in tick-derived cell lines. ISE6 cells on coverslips were infected with M. leprae Thai-53 strain. Infected and uninfected samples at 21, 35, and 49 dpi were prepared for TEM imaging. Images were captured using TEM microscope and bacilli were indicated by red arrowheads.   ticks further supports the likelihood of horizontal transmission.
The results of the current study are consistent with previous examination of A. sculptum transmission of M. leprae to rabbit hosts (Ferreira et al., 2018). Combined, employing different tick life cycle stages (nymph vs. adult) and inoculation routes (injection vs. feeding), these results demonstrate that ticks can deliver M. leprae via saliva during blood meal acquisition. Further studies are needed to determine if ticks can acquire or transmit M. leprae while feeding on zoonotic hosts.
A major obstacle to laboratory research on M. leprae is associated the difficulty to propagate the organisms in cell culture and the slow doubling time (∼14 days). Utilization of protozoan-and arthropod-derived cell lines have provided an intriguing observation for M. leprae cultivation that merits additional consideration (Ferreira et al., 2018). In the current study, M. leprae Thai-53 strain was sustained in either tickderived ISE6 cells at 30 • C for up to 49 days. Previously, the ability of tick-derived cells to support viable M. leprae Thai-53 identified a cell origin-dependent susceptibility to infection for a 20-day period, with an increase in viable M. leprae in Ixodesderived (IDE8) cell line (Ferreira et al., 2018). Despite using Ixodes-derived cell lines in both the Ferreira et al. (2018) and the current study, several variables including cell line origin, times of assessment, inoculum dose, and viability RT-qPCR assays likely account for the differences between the two studies. The 16s rRNA/16s rDNA assay employed in the previous study does not account for a minimal threshold of bacteria to assess viability and the residual stability of 16srRNA (Martinez et al., 2009). The viability assay used in the current study requires at least 3,000 bacteria to be present in the reverse transcription reaction in order to evaluate viability (Davis et al., 2013). This assay has been validated by direct comparison with other established viability assays for M. leprae, including radiorespirometry, live/dead staining, and mouse foot pad assay. More studies are required to discern if other variables, including host cell background, contribute to the growth kinetics of M. leprae in vitro.
Comparison of intracellular and extracellular preparations identified that while M. leprae DNA was mostly associated with host cells, viability of M. leprae was greater in extracellular supernatant. Because half of the media was replaced on weekly intervals, it is possible that some bacilli were removed from the samples over the course of culture. Subsequent studies should recover any bacilli that may be extracellular and return to the culture during media exchanges to better quantify extracellular M. leprae. Bioinformatic analysis indicates that all anabolic pathways of M. leprae are intact but non-functional in synthesizing their own energy (Singh and Cole, 2011); therefore, the growth of mycobacteria are restricted by carbon sources and catabolism (Cole et al., 2001;Wheeler, 2001). The L15B media for tick cells is composed of amino acids, tricarboxylic acid, tryptose phosphate, glucose, minerals, vitamins and heat inactivated bovine serum (Munderloh and Kurtti, 1989). The abundant nutrient source can support extracellular survival of other obligate intracellular bacteria including Rickettsia (Sunyakumthorn et al., 2008), consistent with what was observed with M. leprae for 49 dpi in the current study.
In order to demonstrate M. leprae viability, MFP test as gold standard is currently an essential tool in mycobacterium research (Ng et al., 1973;Levy and Ji, 2006). In the current study, after >300 days in culture, both strains of M. leprae were able to replicate in MFP, suggesting that non-propagative culture still allows for maintenance of viable bacilli. Ticks can maintain obligate intracellular bacterial populations for an extended life cycle, sometimes up to two years, and transovarially transmit bacteria to their progeny (Laukaitis and Macaluso, 2021). The isolation and cultivation of the M. chelonae complex bacilli in tick cells derived from eggs of field-caught ticks (Palomar et al., 2019) supports the current in vitro studies demonstrating Mycobacterium persistence in tick cells which is consistent with the previous study (Ferreira et al., 2018), M. leprae does not show sustainable growth in ISE6 cells. The extended culture of M. leprae in tick cells (>700 days) provided an opportunity for visual and molecular characterization. Rare bacilli within tick cells were observed by both acid-fast staining and TEM and observations on M. leprae morphology suggest that viable stained bacilli form a rod shape, with dying or dead bacilli commonly appearing as a shorter beaded or granular shape (Scollard et al., 2006). Another observation in prolonged passage of M. lepraeinfected tick cell culture included the presence of black spheres resembling virus when assessed by TEM. Embryo-derived tick cell lines commonly contain endogenous viruses (Bell-Sakyi and Attoui, 2013); however, the characterization and potential influence of co-infections on M. leprae viability or maintenance remain to be examined.
Strain and/or species variation in other tick-borne obligate intracellular bacteria is associated with differences in phenotype in tick cell culture (Bell-Sakyi et al., 2018). After extended culture of M. leprae strains Thai-53 and Br4923 in tick-derived cells for ∼300 days, strain Br4923 grew to higher levels in mouse footpads than Thai-53. This would suggest that Br4923 had a higher percent survival rate in the tick cells. Interestingly, when compared head-to-head in an armadillo model, Br4923 likewise exhibited more prolific growth than Thai-53 (Sharma et al., 2018). Although whole genome sequence analysis of these geographically distant M. leprae strains suggest they are identical with over 99.995% genome sequence identity in terms of order of genes, sequences, and with no mutations; inversion, translocation, duplication, or transpositions (Monot et al., 2009;Singh and Cole, 2011), maintenance of M. leprae in ISE6 cells may select for pathological variations between M. leprae strain types. Alternatively, latency, as seen in other Mycobacterium spp. including Mycobacterium tuberculosis, is a non-metabolically active stage (Cardona and Ruiz-Manzano, 2004) and may have been induced in a strain-specific manner after prolonged culture. However, the ability of M. leprae to limit replication in extreme conditions has been shown as bacilli survive in amoebal cysts for months and recover to a replicative state when returned to favorable conditions (Wheat et al., 2014). Nevertheless, the Thai-53 strain recovered from ISE6 cells multiplied once inoculated into the mouse footpads and, like Br4923, produced a robust normalized esxA signal indicative of highly viable M. leprae.
In summary, the results of the current study demonstrate ticks can harbor M. leprae when introduced via needle inoculation. Likewise, the ability to maintain viable and infectious M. leprae under culture conditions using an arthropod-derived cell line was exhibited. Identifying the correct pairing of tick cell line and bacterial strain that will facilitate propagation of bacteria will advance the field with respect to molecular biology and therapeutic advances. Similarly, the prolonged culture of M. leprae for nearly one year can serve as a model to investigate the biology of pathogen persistence. Although untested in the current study, the evidence for ticks that parasitize both zoonotic reservoir hosts and humans to act as a vector for M. leprae is apparent and should be further examined using a biologically relevant transmission/disease system.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

ETHICS STATEMENT
The animal study was reviewed and approved by all animal procedures were performed in accordance with the United States Public Health Service Policy on the Humane Care and Use of Laboratory Animals. The use of vertebrate animals for tick feeding was reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at LSU-SVM (protocol 17-001). The use of mice for the infectivity assay was reviewed and approved by NHDP IACUC (Assurance # D16-00019 [A3032-01]).