Multifunctional pMGF505-7R Is a Key Virulence-Related Factor of African Swine Fever Virus

African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), and it is an enveloped, icosahedral, double-stranded DNA virus with a genome length ranging from 170 to 193 kb. The ASFV genome contains at least five multigene families (MGFs): MGF100, MGF110, MGF300, MGF360, and MGF505 at the right and left terminal variable regions. The members of the same MGF family are most similar and have conserved sequence motifs, whereas the genetic diversity of different MGF families varies widely. MGF genes play a crucial role in determining ASFV host range, virulence and reducing early cell death post-infection. pMGF505-7R is a multifunctional protein. Recent research advances of pMGF505-7R provide a few new clues to understand the functions of pMGF505-7R either in antagonizing the induction of type I IFN production and IFN downstream signaling or in suppressing inflammatory responses by inhibition of NF-κB signaling and NLRP3 inflammasome, which may be related to ASFV infection-induced pathogenesis.

FIGURE 1 | ASFV pMGF505-7R is a multifunctional protein involved in viral pathogenesis. (Golding et al., 2016). Consistently, a study revealed that the enhanced type I interferon (IFN-I) responses were observed in the PAMs infected with a recombinant virus Pr4 35 derived from the highly virulent strain Pr4 with deletion of MGF360 and MGF530 (Afonso et al., 2004). Subsequently, many recombinant viruses with deletion of MGF360 and MGF530 from three different genotypes also reduced virulence in pigs (O'Donnell et al., 2015). Until now, the functions of the members of these MGF families have not been well characterized.

AFRICAN SWINE FEVER VIRUS pMGF505-7R SUPPRESSES IFN-I PRODUCTION
Accumulating evidence suggests that several ASFV-encoded MGF proteins can suppress the IFN-I induction. For example, pMGF360-15R/pA276R was found to inhibit the induction of IFN-I via both TLR3 and the cytosolic sensing pathways by inhibiting IRF3 through a mechanism independent of IRF7 and NF-κB (Correia et al., 2013). pMGF505-7R/pA528R was found to inhibit the induction of IFN-I through the inhibition of IRF3 and NF-κB (Correia et al., 2013). Recently, ASFV pMGF505-7R was found to exert a negative regulatory effect on the cGAS-STING signaling pathway at the STING level (Li et al., 2021a). An ASFV-MGF505-7R recombinant virus derived from ASFV CN/GS/2018 strain induced more IFN-β production than wild-type ASFV infection in PAMs. Mechanistically, pMGF505-7R promoted the expression of the autophagy-related protein ULK1 to degrade STING (Li et al., 2021a). Li et al. (2021c) also noticed that pMGF505-7R interacted with and inhibited the nuclear translocation of IRF3, resulting in blocking IFN-I production. Of note, the replication ability of ASFV-MGF505-7R was reduced compared with wild-type ASFV in vivo, and the ASFV-MGF505-7R virus was fully attenuated in pigs. Altogether, these results showed that MGF505-7R may be a new key virulencerelated factor.

AFRICAN SWINE FEVER VIRUS pMGF505-7R MODULATES THE IFN SIGNALING PATHWAY
Treatment of ASFV-infected cells with IFN-I did not reduce the replication of virulent ASFV isolates. Consistently, ASFV lacking the gene of MGF360 and MGF505 families is partially sensitive to IFN-I compared to its parent virus (Golding et al., 2016). These results suggest that the ASFV developed strategies to inhibit IFN-I induced host antiviral state. On the other hand, it is well known that the binding of secreted IFN-II (γ) to their respective receptors (IFNGR1/IFNGR2) in the viralinfected cells or neighboring cells initiates the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway. The process leads to the phosphorylation of STAT1 by JAK1 and JAK2, resulting in formation of a STAT1 dimer which then translocates to the nucleus to activate transcription of IFN-regulated genes including IFN stimulated genes (ISGs). These ISGs have a variety of antiviral activities, including the production of chemokines and other proteins involved in activating the innate and adaptive responses to restrict virus replication (Mazewski et al., 2020).
It has been reported that ASFV infection suppresses JAK-STAT signaling to evade the immune response. For example, pMGF505-7R was reported to inhibit both the type I and type II IFN signaling pathways, limiting the effect of IFN-I and IFN-II on the JAK-STAT signaling pathway and the expression of ISGs (Correia et al., 2013). Recently, Li et al. (2021b) reported that pMGF505-7R from ASFV CN/GS/2018 strain interacts with JAK1/JAK2 and mediates their degradation by up-regulating the E3 ubiquitin ligase RNF125 expression and inhibiting the expression of Hes5. Consistently, ASFV-MGF505-7R derived from ASFV CN/GS/2018 strain induced higher levels of IRF1 expression and displayed compromised replication both in PAMs and pigs compared with wild-type ASFV (Li et al., 2021b). MGF505-7R deficiency attenuated the virulence of the ASFV and pathogenesis of ASF in pigs, suggesting that the MGF-505-7R gene plays a critical role in the virulence of the ASFV and pathogenesis of ASF by antagonizing IFN-I production and IFN (I and II) downstream JAK-STAT signaling.

AFRICAN SWINE FEVER VIRUS pMGF505-7R INHIBITS THE ACTIVATION OF THE NF-κB SIGNALING PATHWAY AND NLRP3 INFLAMMASOME
Inflammatory factors are key components of host antiviral responses released from the pathogens-infected macrophages. Recently, ASFV pMGF505-7R was found to regulate ASFV pathogenicity by inhibiting IL-1β and IFN-I production (Li et al., 2021c). ASFV HLJ/18 isolate infection induced low levels of IL-1β in PAMs, even in the presence of strong inducers such as LPS and poly(dA:dT). Additionally, ASFV HLJ/18 strain infection-induced IL-1β production is depended on TLRs/NF-κB signaling pathway and NLRP3 inflammasome. Subsequently, several members of the MGF360 and MGF505 families were found to significantly inhibit IL-1β maturation and secretion. Among them, pMGF505-7R had the strongest inhibitory effect. Mechanistically, pMGF505-7R not only interacted with IKKα in the IKK complex to inhibit NF-κB activation and but also bound to NLRP3 to inhibit inflammasome formation, resulting in decreased IL-1β production. Consistent with these results, A528R can suppress the NF-κB p65 phosphorylation and nuclear translocation, and the antiviral and antibacterial activity (Liu et al., 2021), while an ASFV-MGF505-7R recombinant virus derived from ASFV HLJ/18 induced higher levels of IL-1β in PAMs compared with its parental ASFV HLJ/18 strain. Importantly, the virulence of ASFV-MGF505-7R is reduced in piglets, which may occur due to induction of higher IL-1β and IFN-I production in vivo.
Although both the two groups confirmed that the virulence of the two ASFV-MGF505-7R recombinant viruses, derived from ASFV HLJ/18 strain and ASFV CN/GS/2018 strain, respectively, were attenuated compared with wild-type ASFV (Li et al., 2021a,b,c). Zheng's group found that the ASFV-MGF505-7R virus derived from the ASFV CN/GS/2018 strain, was fully attenuated in pigs (Li et al., 2021a) while Weng's group showed that nearly 40% of piglets inoculated with the ASFV-MGF505-7R virus derived from ASFV HLJ/18 strain still died, suggesting that this recombinant ASFV-MGF505-7R retains some of its virulence. The differences of experimental results between the two groups may be caused by the usage of different ASFV isolates with different genetic backgrounds or by using different does of virus to challenge the pigs, and the specific reasons still need to be further investigated in the future.

CONCLUSION
Taken together, pMGF505-7R is a multifunctional protein.
Recent research advances of pMGF505-7R provide a few new clues to understand the functions of pMGF505-7R either in antagonizing the induction of IFN-I and IFN downstream signaling or in suppressing inflammatory responses by inhibition of NF-κB signaling and NLRP3 inflammasome (Figure 1), which may be related to ASFV infection-induced pathogenesis. pMGF505-7R, as a multifunctional protein, may have other functions not found, and needs to be further explored. The current progress in understanding of pMGF505-7R might help design antiviral agents or live-attenuated vaccines to control ASF.

AUTHOR CONTRIBUTIONS
CW and LH wrote the manuscript. LH and JZ drew the diagram. JL and DL modified the manuscript. All authors contributed to the article and approved the submitted version.

FUNDING
This study was supported by the National Natural Science Foundation of China (31941002).