The Deterioration of Agronomical Traits of the Continuous Cropping of Stevia Is Associated With the Dynamics of Soil Bacterial Community

Stevia rebaudiana Bertoni is grown worldwide as an important, natural sweetener resource plant. The yield of steviol glycosides (SVglys) is greatly influenced by continuous cropping. In this study, we collected the roots, rhizosphere soils, and bulk soils from 2 years of continuous cropping (Y2) and 8 years of continuous cropping (Y8). A high-throughput sequencing technology based on Illumina Hiseq 2500 platform was used to study the structure and diversity of bacterial communities in the roots and soils of stevia with different years of continuous cropping. The results demonstrated that although the content of a group of SVglys was significantly increased in stevia of long-term continuous cropping, it inhibited the growth of plants and lowered the leaf dry weight; as a result, the total amount of SVglys was significantly decreased. Meanwhile, continuous cropping changed the physicochemical properties and the bacterial composition communities of soil. The different sampling sources of the root, rhizosphere soil, and bulk soil had no impact on the richness of bacterial communities, while it exhibited obvious effects on the diversity of bacterial communities. Continuous cropping had a stronger effect on the bacterial community composition in rhizosphere soil than in root and bulk soil. Based on linear discriminant analysis effect size (LEfSe), in the rhizosphere soil of Y8, the relative abundance of some beneficial bacterial genera of Sphingomonas, Devosia, Streptomyces, and Flavobacterium decreased significantly, while the relative abundance of Polycyclovorans, Haliangium, and Nitrospira greatly increased. Moreover, the soil pH and nutrient content, especially the soil organic matter, were correlated with the relative abundance of predominant bacteria at the genus level. This study provides a theoretical basis for uncovering the mechanism of obstacles in continuous stevia cropping and provides guidance for the sustainable development of stevia.

Stevia rebaudiana Bertoni is grown worldwide as an important, natural sweetener resource plant. The yield of steviol glycosides (SVglys) is greatly influenced by continuous cropping. In this study, we collected the roots, rhizosphere soils, and bulk soils from 2 years of continuous cropping (Y2) and 8 years of continuous cropping (Y8). A high-throughput sequencing technology based on Illumina Hiseq 2500 platform was used to study the structure and diversity of bacterial communities in the roots and soils of stevia with different years of continuous cropping. The results demonstrated that although the content of a group of SVglys was significantly increased in stevia of long-term continuous cropping, it inhibited the growth of plants and lowered the leaf dry weight; as a result, the total amount of SVglys was significantly decreased. Meanwhile, continuous cropping changed the physicochemical properties and the bacterial composition communities of soil. The different sampling sources of the root, rhizosphere soil, and bulk soil had no impact on the richness of bacterial communities, while it exhibited obvious effects on the diversity of bacterial communities. Continuous cropping had a stronger effect on the bacterial community composition in rhizosphere soil than in root and bulk soil. Based on linear discriminant analysis effect size (LEfSe), in the rhizosphere soil of Y8, the relative abundance of some beneficial bacterial genera of Sphingomonas, Devosia, Streptomyces, and Flavobacterium decreased significantly, while the relative abundance of Polycyclovorans, Haliangium, and Nitrospira greatly increased. Moreover, the soil pH and nutrient content, especially the soil organic matter, were correlated with the relative INTRODUCTION Stevia [Stevia rebaudiana (Bertoni) Hemsl.] is a perennial medicinal herb of the family Asteraceae. Steviol glycosides (SVglys) have many advantages including high concentration (∼4-20% of leaf dry weight), high sweetness (∼300-450 times of sucrose), and low heat (close to zero calories) (Karimi et al., 2019). Belonging to a kind of terpenoid mixture, SVglys mainly consist of Stevioside (STV) and Rebaudioside series; among them, STV and Rebaudioside A (RA) are higher than other ingredients (Tavarini et al., 2015). SVglys have the characteristics of easy dissolution, heat resistance, stability, harmfulness, and certain adjuvant therapeutic effects on diabetes, obesity, and dental caries (Díaz-Gutiérrez et al., 2020). With the widespread usage of SVglys in food, beverage, and other industries as a natural sweetener, stevia is widely cultivated in the world as a new source of sugar. The continuous cropping inevitably occurs because the plant is normally grown around the processing factories for SVgly extraction. However, continuous cropping usually leads to poor crop growth, low yield, poor quality, and susceptibility to diseases (Yang et al., 2011;Wacal et al., 2019;Liu et al., 2020), which seriously restricts the sustainable cropping. Soil factors are considered to be the main causes of continuous cropping obstacles, such as nutrient imbalance, property deterioration, and microbial community structure change (Wang et al., 2020;Li et al., 2021).
Soil microorganisms and plant roots interact with each other mainly in the rhizosphere (Qiao et al., 2017). Plant rhizosphere refers to the soil around the root system that changes physicochemical and biological characteristics due to the growth and activity of plant roots (Berendsen et al., 2012). This is a highly diversified microbial habitat, in which, plants rely on the exchange between roots and rhizosphere microorganisms to obtain nutrients, promote growth, and inhibit diseases (Wu et al., 2015;Garcia et al., 2016). Rhizosphere bacteria with the most abundant are beneficial to the soil to remain in a healthy and productive state (Li et al., 2019). Apart from soil fertility, continuous cropping also changes the quantity and activity of rhizosphere microorganisms (Castrillo et al., 2017;Wang et al., 2018). For example, the relative abundances of the Firmicutes, Actinobacteria, Bacteroidetes, and Basidiomycota phyla were depleted during years of vanilla monoculture (Xiong et al., 2015). Similarly, after continuous cropping of Radix pseudostellariae, Pseudomonas spp., Burkburkella spp., and Bacillus pumilus in the rhizosphere decreased significantly (Wu et al., 2019), while the Fusarium genus, one major pathogen of plant root rot, showed increasing tendency with continuous cropping of American ginseng (Panax quinquefolium L.) (Fan et al., 2021). These observations indicated that continuous cropping destroyed the balance of original microbial flora and resulted in the imbalance of rhizosphere microecology (Dong et al., 2016;Li et al., 2021), which would have an important impact on the quality of medicinal plants. Zhu et al. (2021) found that different soil microenvironments significantly affected the growth and active components of Angelica sinensis, and the key bacterial species may be the key factor determining the quality of A. sinensis, which indicated that the soil microenvironment contributed to the growth and accumulation of active components of medicinal plants.
High-throughput sequencing provides a new strategy for the study of rhizosphere microbial communities (Xia et al., 2019). By performing large-scale soil microbial sequencing, the composition and distribution of bacteria colonized in the plant's roots and soil can be explored. Moreover, the changes in bacterial community components at different taxonomy levels can be traced (Wang et al., 2021). So far, an in-depth understanding of soil bacterial community diversity in stevia, especially for the situation of continuous cropping, is yet to be explored. Therefore, based on 16s rDNA high-throughput sequencing technology, this study aimed to investigate the differences in bacterial community composition and the structure in the rhizosphere of stevia after short-term and long-term continuous cropping, as well as to analyze the possible mechanisms and the impacts on rhizosphere of ecological processes. This study also tried to explore the relationship between the bacterial community and soil environmental factors, which provided valuable clues for bioremediation of continuous cropping obstacles of stevia.

Site Description and Experimental Design
The study area was located in Guardian Town, Mingguang City, Anhui Province, China (32 • 77'N, 117 • 99'E) with an altitude of 30 m above sea level. The climate belongs to the transition zone between subtropical and temperate zones. The average annual temperature is 15 • C, the annual rainfall is 940 mm, the annual sunshine is 2,210 h, and the frost-free period is 219 days. The test site was a continuous cropping field of stevia, where no other crops were planted before or after stevia harvest each year. This experiment was set for 2 years of continuous cropping (Y2) and 8 years of continuous cropping (Y8), which were performed in sandy loam. The physicochemical properties of Y2 were tested with pH 8.21, total nitrogen (TN) 1.19 g/kg, organic matter (OM) 14.86 g/kg, available phosphorus (AP) 93.41 mg/kg, and available potassium (AK) 72.00 mg/kg; while for the Y8, the corresponding profiles were pH 7.57, TN 1.10 g/kg, OM 13.25 g/kg, AP 72.61 mg/kg, and AK 80.33 mg/kg. Before transplanting, nitrogen, phosphorus, and potassium compound fertilizer (N:P 2 O 5 :K 2 O = 15:15:15, 750 kg/ha) and rotten chicken manure were applied as base fertilizer, and 300 kg/ha compound fertilizer was applied after transplanting.

Collection of Soil and Plant Samples
The samples were collected before the flowering stage of stevia, and 18 plants were randomly selected for the sampling method ("S" shape) for 6 replicates (3 plants for each replicate). During the sampling, the root and the soil around the root were all excavated with a spade and immediately put into an icebox, which was transported to the laboratory for subsequent experiments. The soil shaken off was used as bulk soil (BS), and the soil adhering to the root surface brushed off with a brush was used as rhizosphere soil (RS). The collected soil samples were divided into two parts, one part is for the determination of physicochemical indexes after natural air drying, and the other part is that soil and root samples cleaned with sterile water were placed in a −80 • C refrigerator for DNA extraction. The microbial test samples were named root (Y2.R and Y8.R), rhizosphere soil (Y2.RS and Y8.RS), and bulk soil (Y2.BS and Y8.BS), respectively. At the same time, the leaves of the above-ground plants were taken and dried naturally for SVglys determination.

Analysis of Soil Chemical Properties, Plant Growth, and SVgly Content
The soil samples were sieved through a 2-mm mesh prior to chemical analysis. Soil pH was determined using a pH electrode in soil-water (1:2.5 W/V) after shaking for 30 min. OM, TN, AP, and AK of soil were determined using a previously described method (Shen et al., 2015). Plant height, stem diameter, and leaf dry weight were measured using a meter ruler, vernier caliper, and electronic balance, respectively. The soil and impurities attached to the root system were removed and rinsed with clean water. Root pictures were obtained after scanning using a scanner (Epson V700), and indicators of root length, root surface area, root diameter, and root volume were obtained using the WinRHIZO root analysis system (Pro2007d). The leaf sample was ground thoroughly in a mortar. After passing through a 60-mesh sieve, accurately weighed 50 g of the sample, added 1.5 ml of 50% ethanol, ultrasonically extracted for 40 min, centrifuged at 10,000 r/min for 10 min, the supernatant was filtered by 0.45 µm organic microporous membrane, and the contents of STV, RA, Rebaudioside C (RC), Rebaudioside D (RD), Rebaudioside M (RM), and Rebaudioside F (RF) were detected according to Liu et al. (2010).

DNA Extraction, Amplification, and Sequencing
The total DNA was extracted from root and soil samples using the Minkagene Plant DNA Kit and Advanced Soil DNA Kit, respectively. NanoDrop was used to detect DNA concentration and purity. The amplification primers were 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'), and the 16S rDNA V3-V4 region sequences were amplified. The polymerase chain reaction (PCR) amplification system was as follows: 25 µl 2X Premix Taq, 1 µl Primer-F (10 mM), 1 µl Primer-R (10 mM), 3 µl DNA (20 ng/µl), and 20 µl Nuclease-free water. PCR reaction parameters were as follows: 94 • C denaturation for 5 min, 94 • C for 30 s, 52 • C for 30 s, 72 • C for 30 s, a total of 30 cycles, and extension at 72 • C for 10 min. The concentrations of PCR products were compared using the GeneTools analysis software (version 4.03.05.0, SynGene). The required volumes of each sample were calculated according to the principle of equal mass, and the PCR products were mixed. Reagent for gel recovery using E.Z.N.A. R Gel Extraction Kit and target DNA fragments were recovered using TE buffer elution. The library was built according to the NEBNext R Ultra TM DNA Library Prep Kit for the Illumine R standard process. The library was sequenced on an IlluminaHiseq2500, and 250 bp paired-end reads were generated (Guangdong Magigene Biotechnology Co., Ltd., Guangzhou, China).

Bioinformatics and Statistical Analyses
The raw sequenced data were analyzed according to the Qiime2 platform (version 2020.11.0) (Bolyen et al., 2019). After optimizing statistics and removing impurities, the original sequencing data were flatted, and then the effective sequences were clustered into the operational taxonomic unit (OTU) according to 97% similarity using Usearch (version 10.0.240; http://www.drive5.com/usearch/) (Edgar, 2010) with default parameters. The taxonomic assignment for each OTU was carried out using Usearch -sintax with a cutoff of 0.8. The search database for the assignment was the SILVA 16S rRNA database (Quast et al., 2013). The taxonomic assignment results were shown in seven levels (i.e., Kingdom, Phylum, Class, Order, Family, Genus, and Species). Alpha diversity (Chao richness and Shannon's diversity) statistics for each sequencing sample was performed using Usearch alpha_div (version 10.0.240). Statistical analysis of the sequencing data was conducted in the R environment (version 3.5.1). Beta diversity (principal coordinate analysis, PCoA) based on Bray Curtis dissimilarities was performed using R package "vegan". We performed LEfSe analysis to discover the significant differences in microbiota taxa between the long-and shortterm groups. In this study, LEfSe analysis was carried out using microbiota taxa in phylum and genus level, and taxa were identified with statistically significant (p < 0.05) and linear discriminant analysis (LDA) score > 3. The canonical correspondence analysis (CCA) was used to evaluate the effects of soil physicochemical properties on microbial community structure. The SAS 9.12 was used for the analysis of variance and correlation, and the least significance difference (LSD) method was used for multiple comparisons with the significance level set to p < 0.05. The graphs were created using GrapPad Prism 7.

Effect of Continuous Cropping on Plant Growth, SVgly Content, and Soil Physicochemical Properties
The continuous cropping severely inhibited the growth of stevia ( Figure 1A). Except for root diameter, other plant growth parameters, namely, plant height, stem diameter, branching number, leaf dry weight per plant, root length, root surface area, root diameter, root volume, and yield of Y8 were significantly lower (p < 0.05) than that of Y2 (Figures 1B-J).
Although the content of SVglys in Y8 was higher than in Y2 (Figure 1K), the lower biomass still determined its lower total amount ( Figure 1L). In addition, the soil TN, AP, and AK contents significantly decreased, whereas soil pH significantly increased (p < 0.05) after 8 years of continuous cultivation (Figure 2).

Impacts of Continuous Cropping on Bacterial Community Composition in Plant's Root and Soil
In this study, there were 6,443 distinct OTUs observed among all subsamples, which were from an average of 460,261 sequences across each subsample. The results showed that the number of OTUs in the root was lower than that in rhizosphere soil and bulk soil ( Figure 3A). The overlap number of OTUs in the Venn diagram reflected the correlation degree of bacterial diversity. The total number of OTUs in Y2 was 6,059, and the specific OTUs in the root, rhizosphere soil, and bulk soil accounted for 0.36, 8.57, and 16.04%, respectively. The total number of OTUs in Y8 was 6,137, of which the specific OTUs in the root, rhizosphere soil, and bulk soil accounted for 0.33, 9.71, and 13.28%, respectively. In addition, the OTU numbers that were commonly detected among root, rhizosphere soil, and bulk soil accounted for 15.58 and 15.46% in Y2 and Y8, respectively. Overall, the number of bacterial species detected in roots was much lower than that in soil samples, and they diverged across different years of continuous cropping ( Figure 3B). The Chao richness and Shannon's diversity were calculated to further investigate the community richness and diversity across each sample. The microbial richness in roots was lower than that in soil samples separately in both Y2 and Y8. Across different continuous cropping years, the richness has no significant differences in the root, rhizosphere soil, and bulk soil (Figure 3C). The microbial richness varied along with the continuous cropping but without significance, while the species diversity values were significantly different in the root and rhizosphere soil ( Figure 3D).
The PCoA was performed to compare the similarity or dissimilarity of bacterial communities among different samples ( Figure 4A). The two main coordinates explained 47.00% of the microbial community changes among all the samples, of which PC1 explained 35.4% of the variation and PC2 explained 11.6% of the variation. Meanwhile, PCoA showed that all the samples were grouped into three clusters. The root samples, rhizosphere soil samples, and bulk soil samples between Y2 and Y8 were much closer due to their similar bacterial community structure. The root samples differed mostly from soil samples, and the two root samples were not farther apart. This indicated that soil bacterial community structures were distinctly different from the root samples, and it suggested that the structure of the soil bacterial community was affected by continuous stevia cropping. To illustrate the above results, we further investigated the abundance divergence of microbiome compositions among all the six groups in six taxonomy classification levels, and only the rhizosphere soil showed significant differences between Y2 and Y8 (Supplementary Table 1).
The abundance distribution of bacterial composition in each group at the phylum and genus levels is shown in Figures 4B,C. At the phylum level, the top 10 bacterial phyla (>0.1%) in Y2 and Y8 were Proteobacteria, Bacteroidetes, Actinobacteria, Actinobacteria, Chloroflexi, Verrucomicrobia, Firmicutes, Gemmatimonadetes, Planctomycetes, and Patescibacteria. It was noteworthy that all the phyla showed significant differences among the six treatments except for Proteobacteria (p < 0.05). The relative abundance of Actinobacteria, Chloroflexi, Gemmatimonadetes, and Planctomycetes in bulk soils was significantly higher than in roots and rhizosphere soils, while Actinobacteria exhibited the opposite trend. In addition, only Actinobacteria, Gemmatimonadetes, and Planctomycetes in rhizosphere soils showed significant differences between Y2 and Y8, the same as Patescibacteria in roots. At the genus level, Bacillus, Pseudomonas, Streptomyces, MND1, Flavobacterium, Sphingomonas, Devosia, Ramlibacter, Nitrospira, and Ensifer were the top 10 genera, followed by Pseudarthrobacter,

Identification of Dominant Bacteria That Are Related to Continuous Cropping
To gain more insights into the variation in microbial abundance and composition during the continuous cropping, we narrowed our focus to detect differences at the genus level between Y2 and Y8. The LEfSe identified differently abundant taxa between groups and estimated each significantly different taxon's effect size. The LEfSe was performed to identify a specific genus and to search for a statistically significant biomarker at the genus level. The results (Figure 5) showed 11, 30, and 11 bacterial taxa with significant differences in root samples, rhizosphere soils, and bulk soils, respectively. For the Y2 group, Devosia in roots, Streptomyces in bulk soils, and Streptomyces, Flavobacterium, Sphingomonas, Glycomyces, and Niastella in rhizosphere soils were the most abundant types of genera, respectively, whereas for the Y8 group, the most abundant ones were Roseburia in the root, Haliangium in bulk soils, and Polycyclovorans, Haliangium, Methylobacillus, Nitrospira, and Lamia in rhizosphere soils, respectively.

Correlation Analysis Between Soil Microbiome Composition and Soil Environmental Variables
Canonical correspondence analysis (CCA) at the OTU level reflected the relationship between physicochemical properties and the bacterial community of soil ( Figure 6A). The four groups were completely separated, and all the soil physicochemical properties significantly affected the bacterial community structure, and the correlation coefficients were 0.929 (pH), 0.922 (AK), 0.881 (AP), 0.765 (TN), and 0.709 (OM), respectively (Supplementary Table 2). The heatmap based on Spearman's analysis was used to analyze the relationship between soil physiochemistry properties and the top 20 bacterial genera of rhizosphere soil and bulk soil ( Figure 6B). Group I including Flavobacterium, Devosia, Hydrogenophaga, Ensifer, Sphingomonas, Streptomyces, Bacillus, Dongia, Pseudarthrobacter, Methylotenera, and Lechevalieria presented the negative correlation of pH and the positive correlation of OM. AP, TN, and AK did not affect the relative abundance of bacteria in group I, except Sphingomonas and Streptomyces. Compared with group I, group II, including Polycyclovorans, Haliangium, Bryobacter, Pseudomonas, MND1, and Nitrospira, exhibited the opposite trend, and their relative abundance was positively correlated with pH and was negatively correlated with OM. Besides, AP, TN, and AK had no impact on the relative abundance of bacteria in group II, except Polycyclovorans and Haliangium.

DISCUSSION
Continuous cropping significantly inhibited the growth of stevia and the yield of SVglys; and soil deterioration plays an unquestioned role (Tan et al., 2021). Except pH, the OM, TN, AP, and AK of Y8 were all significantly lower than that of Y2 in the rhizosphere soil, and similar trends were observed in bulk soil except for OM. Previous studies have shown that soil pH exerted a strong influence on the structure of soil microbial communities, and it correlates with the diversity and richness of soil bacterial communities found in different ecosystems (Fierer and Jackson, 2006;Lauber et al., 2009;Tripathi et al., 2012). Future experiments with more continuous cropping years of stevia might help in clarifying the potential of soil pH to regulate the soil bacterial community.
In this study, the total number of OTUs found in the stevia root was significantly lower than in the soil, and the OTUs shared by root, rhizosphere soil, and bulk soil were close in both Y2 and Y8. It suggested that continuous cropping did not affect the stability distribution of bacteria between root and soil, which might be explained by the structural stability of bacterial communities in different niches of plants (Beckers et al., 2017). Moreover, a significantly higher difference value of the OTU number was found in the rhizosphere soil than in the root and the bulk soil, suggesting that a broad diversity of bacterial activities is enhanced in strict contact with plant roots (Li et al., 2014). The small differences in the number of unique OTUs of roots and bulk soils between Y2 and Y8 can be ascribed to the fact that the rhizosphere soil exhibits intense biological and chemical activities (Prashar et al., 2014), which is consistent with the soil physicochemical properties.
Root endophytes are an important part of the plant root microecosystem, and the root microbiome is predominantly assembled from the external microbes in the soil (Edwards et al., 2015). Obviously, the roots and soil bacterial communities were clearly separated from each other, indicating a significant impact of stevia roots on selecting and shaping the rhizosphere bacterial community. Previous study indicated that the diversity of endophytic bacteria in roots of Panax notoginseng was significantly lower than in rhizosphere soil and had less change in the planting time (Tan et al., 2017), which was similar to the results of our study. Moreover, soil environmental factors are the main driving forces for the change in microbial communities (Jiao et al., 2016). Continuous cropping affects the diversity rather than the richness of soil bacteria, which might be due to the selectivity of soil microorganisms in the soil environment. The specific environment became more suitable for the survival of specific bacteria, such as Bacteroidetes and Firmicutes, which were enriched in nutrient-rich soil and poorly structured soil, respectively (Marasco et al., 2013;Griffith et al., 2017). In the previous study, the diversity of Nitrospirae and Elusimicrobia in rhizosphere soil of diseased plants was higher after continuous cropping of Atractylodes macrocephala (Yuan et al., 2019); Planctomycete, one soil oligotroph (Fierer et al., 2007), was found in environments with pH values ranging from below 3 to above 11 (Bohorquez et al., 2012), which was difficult for the plant to grow. Coincidently, Nitrospirae, Elusimicrobia, and Planctomycete of rhizosphere soil significantly increased after Y8, which was also consistent with the decrease in soil nutrient content after Y8.
At the genus level, continuous cropping also changed the relative abundance of predominant bacteria. In the previous studies, Streptomyces (Actinobacteria) is the most important bacterial community in soil and has great potential for bioremediation of toxic compounds (Álvarez et al., 2012); Flavobacterium (Bacteroidetes), a kind of plant growthpromoting bacteria (PGPB), exhibits significant biocontrol activity and affects many events occurring during the plant life cycle (Jeong et al., 2019;Kim et al., 2020); Sphingomonas (Bacteroidetes) resisted plant diseases and promoted plant growth (Khan et al., 2014;Zhou et al., 2016); and Devosia is well known for its dominance in soil habitats contaminated with various toxins and is best characterized for its bioremediation potential (Talwar et al., 2020). Unfortunately, our study showed that the above four genera were not abundant in Y8. The desolating microbial populations decrease soil productivity and crop yields over time. In addition, Polycyclovorans (Proteobacteria) may be the obligate hydrocarbonoclastic bacteria and exhibit a narrow nutritional spectrum (Thompson et al., 2018), which is considered to be the bacterial host related to soil pollution (Costeira et al., 2019); Haliangium is sensitive to antibiotic disturbance (Uddin et al., 2019), and it produced antifungal substances that act against phytopathogens (Ma et al., 2018); Methylobacillus was involved in polycyclic aromatic hydrocarbon (PAH) degradation and might have the ability to restore the contaminated soil (Lu et al., 2019). In this study, the relative abundance of Polycyclovorans, Haliangium, and Methylobacillus significantly increased in rhizosphere soil after 8 years of continuous stevia cropping. It suggested that the adverse effects caused by long-term continuous cropping might be mitigated by the increase of the above degrading bacteria. Notably, as a kind of beneficial bacteria, Nitrospira is significantly influenced by soil pH, exhibiting the strongest biological activity under pH neutral or slightly alkaline conditions, and its abundance is easily inhibited by a high nutritional environment (Simonin et al., 2015;Bai et al., 2020;Yin et al., 2021). Soil with the highest pH and lower nutrient content after 8 years of continuous stevia cropping may explain the significant increase of Nitrospira in soil.
Soil ecosystems are formed by complex interactions between biological communities and physicochemical variables, which jointly determine the overall quality of soil (Hermans et al., 2020). The complex regulatory network of the soil bacterial community is mainly related to soil fertility and ecological function, especially soil pH (Kim et al., 2016;Ren et al., 2018). We concluded that the soil bacterial community was significantly affected by soil physicochemical properties including pH, AK, and AP, which was consistent with previous studies (Rousk et al., 2010;Shen et al., 2013;Li et al., 2019). It also indicated that continuous cropping may interfere with the composition and diversity of the microbial community by affecting soil pH. As a result, continuous cropping played a key role in the composition, chemical properties, and utilization efficiency of the matrix and the imbalance of bacterial community function, and it further exhibited an adverse influence on the growth of stevia and the content of SVglys.
In summary, the long-term continuous stevia cropping changed the bacterial communities in the root and soil, enriched the harmful bacteria, and reduced the beneficial bacteria. Therefore, measures must be taken to restore soil microbial communities (Shen et al., 2021). Results of this study may be used to explore reasonable management measures, such as rational fertilization, microbial fertilizer application, and rotation or intercropping for eventual relief of the long-term monoculture problems of stevia plants. In addition, the stevia rhizosphere exhibited strong impacts on the population of bacteria and fungi (Deng et al., 2018); both bacterial and fungal communities were shaped by the same edaphic factors (Zheng et al., 2019). Thus, the whole microbial communities including bacteria, fungi, and archaea shall be studied in the future, together with the dynamics of plant developmental stages.

CONCLUSION
This study analyzed the variation of rhizosphere bacterial community structure of stevia under Y2 and Y8. The relationship of the bacterial community, soil physicochemical properties, and SVgly content was investigated. Our study indicated that during the long-term continuous cropping, bacterial communities shaped into great compositional variations in the stevia root, rhizosphere soil, and bulk soil. The soil physicochemical properties were also greatly transformed with continuous cropping, and it affected the yield and content of SVglys. Our study provides a theoretical reference for the alleviation of continuous cropping obstacles in stevia.

DATA AVAILABILITY STATEMENT
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm. nih.gov/, PRJNA824437.

AUTHOR CONTRIBUTIONS
XX: validation and visualization. XX and QL: investigation. XX, SJ, and ZH: data curation. QL and XH: project administration. QL, ZH, and XH: funding acquisition. XX, XH, MF, ZX, CY, and ZZ: manuscript writing and editing. All authors contributed to the article and approved the submitted version.

FUNDING
This study was supported by the project Environmental assessment of Stevia rebaudiana production in China (20190012), which was delivered in collaboration with Earthwatch Europe, and the Postgraduate Education Reform and Quality Improvement Project of Henan Province (Yu degree [2018] No. 23). This study was also supported by the Shennongjia Academy of Forestry, Hubei, China (No. SAF202102 to XH) and the Hubei Technology Innovation Center for Agricultural Sciences−2020 key technology research and demonstration project of safe and efficient production of genuine medicinal materials (No. 2020-620-000-002-04 to XH).