First report on the enzymatic and immune response of Metarhizium majus bag formulated conidia against Spodoptera frugiperda: An ecofriendly microbial insecticide

Entomopathogenic fungi from microbial sources are a powerful tool for combating insecticide resistance in insect pests. The purpose of the current study was to isolate, identify, and evaluate bag-formulated entomopathogenic fungal conidial virulence against insect pests. We further investigated the enzymatic responses induced by the entomopathogenic fungi as well as the effect on a non-target species. Entomopathogenic fungi were isolated from the Palamalai Hills, India, using the insect bait method, and the Metarhizium majus (MK418990.1) entomopathogen was identified using biotechnological techniques (genomic DNA isolation and 18S rDNA amplification). Bag-formulated fungal conidial efficacy (2.5 × 103, 2.5 × 104, 2.5 × 105, 2.5 × 106, and 2.5 × 107 conidia/ml) was evaluated against third instar larvae of Spodoptera frugiperda at 3, 6, 9, and 12 days of treatment, and acid and alkaline phosphatases, catalase, and superoxide dismutase enzymatic responses were evaluated at 3 days post-treatment. After 12 days of treatment, non-target assays on the earthworm Eudrilus eugeniae were performed using an artificial soil assay. Results of the bag formulated fungal conidial treatment showed that S. frugiperda had high susceptibility rates at higher concentrations (2.5 × 107 conidia/ml) of M. majus. Lower concentration of 2.5 × 103 conidia/ml caused 68.6% mortality, while 2.5 × 107 conidia/ml caused 100% mortality at 9 days post treatment. Investigation into enzymatic responses revealed that at 3 days post M. majus conidia exposure (2.5 × 103 conidia/ml), insect enzyme levels had significantly changed, with acid and alkaline phosphatases, and catalase enzymes significantly reduced and superoxide dismutase enzymes significantly raised relative to the control. After 12 days of treatment, no sublethal effects of M. majus conidia were observed on E. eugeniae, with no observed damage to gut tissues including lumen and epithelial cells, the nucleus, setae, coelom, mitochondria, and muscles. This study offers support for the use of fungal conidia in the target-specific control of insect pests.


Introduction
The Fall Armyworm, Spodoptera frugiperda, is an insect pest of global economic importance, capable of feeding on over 80 different types of crops, including rice, sorghum, millet, sugarcane, cotton, and vegetable crops (Shylesha et al., 2018;Rwomushana, 2019;Pittarate et al., 2021). Of particular importance to the African continent is maize, on which over 220 million people are reliant on this staple crop. For this reason, S. frugiperda represents a major threat to food security. Due to a lack of precise control measures, S. frugiperda is responsible for losses ranging from 8.3 to 20.6 million metric tons per year (Xiao et al., 2020;Pittarate et al., 2021). Unfortunately, synthetic chemicals commonly used in the control of insect pests, although initially promising, result in insecticide resistence in the target pest following repeated use . Furthermore, synthetic chemical pesticides pollute the environment and have negative impacts on non-target organisms. As a result, scientists and researchers are looking to new tools in the control of insect pests that are effective, target-specific, efficacious at low concentrations, and are environmentally friendly (Batta, 2003;Vivekanandhan et al., 2022a,b,c).
Entomopathogenic fungi represent one such promosing tool in the control of insect pests and are used against a variety of agricultural insect pests globally (Singh et al., 2017;Kumar et al., 2019;Liu et al., 2021). The pathogenicity of entomopathogenic fungi begins when spores adhere to the insect's outer cuticle. When conditions are favourable, the conidial spores germinate and penetrate the insect's cuticle. The fungi subsequently secrete toxic chemical constituents into the insect's hemolymph when conidia enter the insect host, defeating the insect's immune system and killing the insect pest (Vivekanandhan et al., 2022c). Of the known entomopathogenic fungi, Beauveria bassiana, Metarhizium anisopliae, B. brongniartii, and I. fumosorosea are currently the most commonly used in insect pest control. However, other species including Nomuraea rileyi, Fusarium oxysporum, and Verticillium lecanii, have also been reported to display high virulence in insect pests (Rao et al., 2006;Vivekanandhan et al., 2022a,b), with many species capable of inducing pathogenicity across the different developmental stages (Feng et al., 1994;Wu et al., 2020). The entomopathogenic fungi B. brongniartii, for example, has been observed to be affective against larval, pupal and adults stages of S. litura under laboratory conditions (Wu et al., 2020), highlighting the potential for entomopathogenic fungal control to target pest insects at various stages of development.
Metarhizium spp., an entomopathogenic fungus with a restricted host range, safety, environmental friendliness, and ease of mass production, has been the subject of extensive research (Schrank and Vainstein, 2010;Canassa et al., 2020;Vivekanandhan et al., 2020Vivekanandhan et al., , 2022aWasuwan et al., 2021). From the arctic to the tropics, the Metarhizum genus is found all over the world. It is a member of the class Hyphomycetes, which reproduces by conidia, or spores (Aw and Hue, 2017 (Thanakitpipattana et al., 2020).
Over the last three decades, a large number of mycoinsecticides based on Metarhizium, as well as Beauveria species, have been commercialised and registered (Wraight et al., 2001;de Faria and Wraight, 2007;Zimmermann, 2007). In many countries, the spores of M. anisopliae, B. bassiana and B. brongniartii have been successfully formulated as mycopesticides, and several of these products have passed registration requirements and are thus currently widely used in the biocontrol of pest insects (Wraight et al., 2001;de Faria and Wraight, 2007). L. lecanii was the first fungus to be developed as an inundated mycoinsecticide for use in medium to large-scale glasshouse farming (Shah and Pell, 2003). Here, the active ingredients from two different isolates were combined to create two commercial products: "Vertalec" for aphid control and "Mycotal" for whitefly and thrip control (Shah and Pell, 2003). Both products are registered in a number of European and non-European countries, including the Netherlands, Finland, Denmark, France, Norway, Turkey, Spain, and the United Kingdom.
When mass-producing entomopathogenic fungi for commercial use, two main methods exist: via a liquid culture or via a solid culture. Conidial production is faster in liquid cultures (fermentation) than in solid cultures. In addition, blastospores are produced in deep agitated liquid cultures. Although blastospores are more delicate and shorterlived than conidia, they have the capacity to be as virulent as conidia (Hall, 1981;Bartlett and Jaronski, 1988;Pham et al., 2009). A sophisticated but commercially viable process can be used to create an effective, long-lasting, wettable powder or suspension concentrate of blastospores (Kim et al., 2013). The majority of the fungal-based pesticides, however, are made with conidia from solid cultures. Conidia must be separated from the cereal substrate on which they were cultured after solid culture in order to formulate powder and liquid products. This is a labour-intensive process that ultimately raises production costs. A conidia-harvesting machine (Mycoharvester) 1 , was produced to overcome this issue, however, the cost of production must be reduced if fungal-based pesticides are to compete with chemicals.
The study entomopathogenic fungus species for the current study is Metarhizium majus; a species capable of growing in low and medium climatic conditions. Indeed, microbial insecticide formulations based on entomopathogenic fungi Metarhizium species are more active at environmental conditions of 20-40°C, with a fungal insecticide life time of 30.8 months (Batta, 2003). Metarhizium majus was originally isolated from the edible insect Protaetia brevitarsis in Korea (Kwak et al., 2021), although has yet to be thoroughly studied for its use as an insecticidal agent. The current study therefore aimed to isolate, characterize, and bag formulations of the entomopathogenic fungi M. majus and investigate the enzymatic and immune responses against S. frugiperda. Toxicity effects were further evaluated on the non-target species Eudrilus eugeniae. Ultimately, the current study aims to increase current understanding on the potential of M. majus for use as an entomopathogenic agent in the target-specific control of insect pests.

Soil sample
Soil samples were collected from the Palamalai Hills, located approximately 25 km north-east from the city centre of Coimbatore, Tamil Nadu, India (11.7355° North, 77.7494° East). Soil samples of 1 kg were collected from a depth of 1-20 cm. Before soil collection, leaf litter and other surface particles were removed according to the method detailed in Vivekanandhan et al. (2020). Collected soil was sealed in a sterile bag and kept at 4°C.

Entomopathogenic fungi isolation
Insect-borne pathogenic fungi were isolated from the soil using the insect bait method . The insect bait method is a very sensitive technique for entomopathogenic fungi isolation. A total of 10 greater wax moth, Galleria mellonella, at the early third instar larvae stage were transferred to a plastic container. The plastic container [11 (L) × 7 (W) × 9 (H) cm], containing 200 g of forest sample soil, was covered with a lid and transferred to the incubator at ambient temperature and relative humidity (27 ± 2°C and 80% RH).
Plastic containers were observed twice a day for 10 days. Every day, dead larvae were collected and sterilised with 70% ethanol for 10 min, and then washed with sterile water. Sterilized insect cadavers were placed on Petri plates (90 × 15 mm) containing PDA medium. Plates were kept at 27 ± 1°C and 85% relative humidity. After 5 days, the pure fungal cultures were separated and stored in a BOD incubator so they could be used in further experiments.

Morphological confirmation
Isolated entomopathogenic fungi were morphologically identified according to Vivekanandhan et al. (2021), based on the fungal colony, color, mycelium shape, and spores. The morphological characteristics of entomopathogenic fungi were evaluated after lactophenol cotton blue (LCB) staining. The glass slides were observed using a light microscope at 40× magnification (Olympus-CH20i/India).

Mass culturing of fungi
To isolate genomic DNA from entomopathogenic fungi, liquid cultures were prepared according to the methods detailed in Vivekanandhan et al. (2021). The culturing flask was filled with 150 ml of potato dextrose broth (PDB) and then sterilised at 120°C using an autoclave for 15 min. The liquid medium was transferred to an aseptic condition (LAF) and 1 × 10 6 conidia/ml was subsequently added to the culture medium. In addition to conidial inoculation, 0.5 ml of chloramphenicol was also added as an anti-bacterial agent. The liquid medium was then mixed and incubated for 7-10 days at 28 ± 1°C to allow for fungal growth.

Molecular identification DNA extraction
After 7-10 days of incubation, Whatman No. 1 filter paper was used to filter the young fungal mycelium (HiMedia, India) and the fungal mat used to extract genomic genetic material. 0.5 g of fungi biomass was broken down for extraction using liquid nitrogen in a sterile mortar and pestle. After the mycelium had been broken down, 2.5 ml of freshly prepared CTAB lysis buffer was added, and the solution transferred to sterile microtubes. Microtubes were incubated in a water bath at 60°C for 60 min. Following incubation, the microtubes were placed at 4°C for 18 min before being centrifuged for 15 min at 8,500 rpm. After centrifugation, the supernatant was transferred to new tubes with an equal amount of chloroform and isoamyl alcohol at a ratio of 24:1 and lightly shaken until an emulsion appeared. Microtubes were then centrifuged at 13,000 rpm for 20 min and the resultant supernatant poured into new tubes. After mixing an equal amount of ice-cold isopropanol with 90% ethanol, micro-tubes were incubated at 25°C for 1 h. Following incubation, micro-tubes were centrifuged at 13,500 rpm for 20 min to collect the genomic DNA pellet in new tubes. Finally, 70% ethanol was used to clean the genomic DNA. Following removal of the ethanol, the purity of the genomic DNA was checked using 0.8% agarose gel electrophoresis.

Fungal sequence examination
At Chromous Biotech Pvt. Ltd. in Tamil Nadu, India, the DNA was sequenced, and the sequence was then submitted to GenBank. By using BLAST analysis, sequence results were compared to the GenBank data bases. Results from the NCBI Genbank database were used to identify the entire species. Following that, the CLUSTAL W programme was used to align the order of the organisms with IN-5. Use of CLUSTALW (BioEdit) (Hall, 1999) for multiple sequence alignments. MegAlign was used to determine the nucleotide and deduced amino acid sequence homology (DNA Star, Inc., Madison, WI, United States). For phylogenetic analysis, FigTree V1.3.1 software was used, along with MEGA5 (Tamura et al., 2011), distance matrix, neighbor-joining (Saitou and Nei, 1987), and maximum parsimony methods (Tamura et al., 2004;Rambaut, 2009).

Rearing of insect culture
Spodoptera frugiperda 1st instar larvae were obtained from a colony established in the laboratory and the date it was established and from where the insects were collected was provided . Larvae were individually cultured on maize in a small plastic cup and maintained under laboratory conditions. All experiments were conducted in the laboratory at 28 ± 1°C and 75-85% relative humidity with a photoperiod of 12L: 12 D.

Solid culture of fungi on maize
Metarhizium majus fungal conidia were grown in a polyethylene bag using maize (500 g). To reduce bacterial contamination, maize (300 g) was placed in a polyethylene bag, and then 100 ml distilled water containing 1 ml citric acid (50% stock solution) was added to the bag. The bag was placed in a water bath at 90 ± 1°C for 1 h before being autoclaved at 12L°C for 15 min. After cooling to room temperature, each bag was inoculated with a 5-ml aliquot of fungal isolate liquid culture. For 2 weeks, the inoculated bags were kept at 28 ± 1°C and a photoperiod of 16L: 8 D. The mycotized maize was dried at room temperature for 2 days to reduce the moisture content to 5%, as measured by a moisture analyser.

Screen bag formulation
The fungal granule containers were made of a burlap bag (10 × 14 cm 2 ) with a pore diameter of 15 m (Alibaba.com, Bangkok, Thailand). To make a screen bag formulation, dried fungal granules (50 g) were packed in the screen bag and closed at the top. To store the finished product, the bag was wrapped in aluminium foil. To make a conidial suspension, the screen bag was immersed in 5 l of distilled water and then agitated to extract the conidia into a 50 ml fresh centrifuge tube (Figure 1). The conidia were then vortexed vigorously for 1 min to homogenise before sieving. The conidial homogenate suspensions were sieved through four layers of sterile cheesecloth and placed in sterile centrifuge tubes to be used later. The concentrations of conidia were measured with a Neubauer hemocytometer under a bright-field microscope (Olympus SZ51/SZ61, India). For the bioassay, five concentrations of 2.5 × 10 3 , 2.5 × 10 4 , 2.5 × 10 5 , 2.5 × 10 6 , and 2.5 × 10 7 conidia/ml were prepared in dilutions.

Larvicidal bioassay
A fungal virulence test was done on the third instar larvae of S. frugiperda by using the maize dip technique. Five different concentrations were prepared from the test solution and were as follows: 1 × 10 5 , 1 × 10 6 , 1 × 10 7 , 1 × 10 8 and 1 × 10 9 conidia/ml. The maize was individually dipped in various concentrations of M. majus fungal conidia. In the test solution, the maize was dipped for 5 s then air dried and placed into a Petri dish. Each Petri dish contained 15 third instar larvae of S. frugiperda. Distilled water was used as a negative control. The larval mortality rate was recorded following 3, 6, 9, and 12 days of treatment. All experiments were conducted in triplicate. The percentage mortality was calculated every 3 days post treatment using Abbott's formula (Abbott, 1925)

Insect enzyme homogenate preparation
Larval midgut tissue was homogenized with 2 ml of PBS buffer and spun at 4°C, 10,000 rpm for 15 min. Cell debris and solid waste were removed, and the supernatant was poured into a clean centrifuge tube, put on ice, and used straight away for enzyme assays.

Acid and alkaline phosphatase assays
Acid and alkaline phosphatase (ACP and ALP) levels were determined using the procedure described by Asakura (1978), with minor modifications. Acid phosphatase activity was determined by thoroughly mixing 0.05 ml of an enzyme source with 2 ml of alkaline buffer solution. The tubes were then incubated for 30 min at 36°C. 2 ml of NaOH solution was added and thoroughly mixed. The absorbance was then measured in a spectrophotometer at 405 nm.
In the alkaline phosphatase activity, 50 mM of sodium acetate buffer (pH; 4.6) was used in place of the alkaline buffer. For 20 μl larvae midgut tissue homogenates, a 500 μl sample of 50 mM Tris-HCl buffer (pH 8.0) was prepared and mixed with an equal volume of the respective buffer containing 12.5 mM p-nitrophenyl phosphate. The enzymatic reaction was stopped after 15 min at 37°C in a water bath by adding 0.5 N NaOH solutions, and subsequently centrifuged (4,000 ×g; 5 min). The absorbance of the resulting clear supernatants was measured at 405 nm. Production of an entomopathogenic fungi M. majus conidia bag formulation. The formulation preparation process and how it is used to make a fungal suspension for spray application are depicted schematically.

Catalase assay
Catalase (CAT) activity was measured using the method by Wang et al. (2001). 2.9 ml of Solution-A (50 mM KPO 4 ; pH 7.0) and Solution-B (0.036% H 2 O 2 , KPO 4 ), and 0.03 ml of H 2 O 2 mixed with phosphate buffer, were mixed with 0.1 ml of larvae midgut tissue homogenate and read against control cuvette containing H 2 O 2 and phosphate buffer only. Absorbance was recorded at 240 nm.

Superoxide dismutase assay
The method of Marklund and Marklund (1974) was used to test superoxide dismutase assay (SOD) activity. 50 μl of a homogenate of larvae midgut tissue was added to new test tubes. The final volume was changed by adding 2.90 ml of 50 mM Tris and 10 mM EDTA, pH 8.2, and then mixing in 100 μl of pyrogallol solution. A UV-visible spectrophotometer was used to measure the absorbance at 440 nm.

Artificial soil assay
Artificial soil for use in the artificial soil toxicity assay was comprised of 27% kaolinite clay, 17% sphagnum peat, and 77% soil in accordance with OECD (1984). Three drops of CaCO 3 were added to regulate the soil pH to 6.0 ± 0.2. Soil water content was maintained within the range of 30 ± 2%. Soil samples were prepared by adding different concentrations of fungal conidia as follows: 2.5 × 10 3 , 2.5 × 10 4 , 2.5 × 10 5 , 2.5 × 10 6, and 2.5 × 10 7 conidia/kg on the basis of dry weight. Fifteen mature E. eugeniae earthworms were transferred to the 1 kg test substrate with different concentrations in test containers, which were covered tightly to prevent the escape of worms. A control group was set up involving the artificial substrate in the absence of a fungal conidia solution. Each replicate contained 15 adults of E. eugeniae, and was replicated in triplicate. The mortality of earthworms was calculated after 3, 6, 9, and 12 days of exposure time.
Histopathological study E. eugeniae were exposed to various concentrations of fungal conidia (treatment group) or no fungal conidia (control group) for 12 days. After 12 days of treatment, control and fungal conidia treated earthworms were fixed in 3% formalin for 3 h at 4°C. Formalin blocks were chilled at 25°C for 3 h, then sliced into 2.5-mm-thick ribbons with 0.5-mm ribbons using a microtome (Leica, Germany). E. eugeniae was stained with Ehrlich's hematoxylin and eosin. After staining, slides were viewed under a light microscope at 40× magnification (Olympus CH20i/Thailand).

Statistical analysis
Mortality of S. frugiperda and E. eugeniae was calculated using the Abbott formula (Abbott, 1925). A one-way ANOVA was used to calculate the variances between treatments, and the Tukey's HSD test was done to categorise the homogeneous types of data sets using SPSS software. In all experiments, the significance level was reported as p = 0.05.

Morphological and molecular identification
The morphological features of isolated entomopathogenic fungi was shown to be light green in colour and rod-like in shape, and produced green conidia (Figure 2). Entomopathogenic fungal genomic DNA was amplified in an optimised state using a universal primer by the 18s rDNA gene, and the amplified DNA fragments were examined using a gel documentation unit. The DNA fragment range obtained was 1,200 bp (Figure 3). The    (Nishi et al., 2015). The neighbourjoining tree method was used to determine the evolutionary closeness of isolated entomopathogenic fungi (Figure 4).

FIGURE 4
The Neighbor-Joining tree method was used to make a phylogenetic tree of the stages of evolution of entomopathogenic fungi that had been isolated. Our isolated fungal strains showed 100% similarity with Metarhizium majus.
Frontiers in Microbiology 07 frontiersin.org entomopathogenic fungi proliferated both inside and outside the insect cadaver ( Figure 5). In a similar study, entomopathogenic fungi Metarhizium majus fungal conidia demonstrated high insecticidal activity against the coleopteran pests Holotricha serrata and Oryctes rhinoceros (Velavan et al., 2017).

Eudrilus eugeniae toxicity assay
Treatment of the terrestrial soil bioindicator species E. eugeneae with various fungal conidial concentrations revealed no lethal toxicity effects. No mortality was observed after 12 days of treatment. According to Zimmermann (2007), the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii have no sublethal toxicity effect on non-target earthworm species.

Histopathology of Eudrilus eugeniae
Sub-lethal toxicity was evaluated in E. eugeniae tissues after 12 days of entomopathogenic fungal conidia treatment. Results showed that fungal conidia induced no damage to gut tissues, namely, the lumen and epithelial cells, nucleus, setae, coelom, mitochondria, and muscles ( Figure 8). The tissues of earthworms treated with the entomopathogenic fungus M. majus conidia did not differ from the tissues of control earthworm. Similarly, to this study, entomopathogenic fungi Beauveria bassiana had no histopathological effect on the earthworm Eisenia fetida (Zhou et al., 2021).

Discussion
Entomopathogenic fungi play an important role in controlling insect pests in an integrated pest management program. The previous study provided a thorough investigation into the use of M. majus, first isolated in 2021 (Kwak et al., 2021), in the control of S. frugiperda. Results revealed that bag-formulated M. majus conidia cause significant mortality in S. frugiperda larvae after 12 days of treatment, whilst leaving the non-target bioindicator species E. fetida unharmed.
Entomopathogenic fungi have been shown to induce mortality across all developmental stages of major insect pests under both laboratory and field conditions and thus represent an important tool in the control of insect pests . M. anisopliae, B. bassiana, V. lecanii, and F. oxysporum fungal conidia, for example, have been shown to be highly virulent against several medical and agricultural insect pests including Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus, Tenebrio molitor and Spodoptera litura (e.g., Roberts and Humber,  Percentage mortality of S. frugiperda after treatment of M. majus conidia for 3, 6, 9, and 12 days under laboratory condition. A M. majus conidia treatment at 3, 6, 9, and 12 days of treatment. Frontiers in Microbiology 08 frontiersin.org 1981; Rai et al., 2014;Sujeetha and Sahayaraj, 2014;Islam et al., 2021). In addition, spores of M. anisopliae, B. bassiana, V. lecanii, C. tropicum, C. lobatum, and L. giganteum induce significant mortality in larvae of the mosquito A. aegypti, A. stephensi, and C. quinquefasciatus (Darbro et al., 2011;Singh and Prakash, 2014;Bharathi et al., 2022). In the present study, M. majus exhibited distinct morphological and molecular characteristics in line with previous studies (Nishi et al., 2015;Sivakumar et al., 2020;Kwak et al., 2021;Mathulwe et al., 2021). M. majus is known to display cylindrical to ellipsoid characteristics, forming candelabrum-like arrangements that create compact conidiophores in a hymenial layer. Mature conidia colonies, which are dark green in color, form chains of equal length within clusters (Mathulwe et al., 2021). Furthermore, bag-formulated M. majus conidia induced significant larvicidal activity against third instar larvae of S. frugiperda, with 100% mortality achieved within 9 days. Dosedependent larvicidal activity was observed, with the highest larvicidal activity achieved at a dose of 2.5 × 10 7 conidia/ml. M. majus fungal pathogenicity and conidial growth were visible on the dead cadavers of S. frugiperda, while in control samples, insect larvae were in good physical shape and no fungal infection was observed. These findings support previous research on species of the genus Spodoptera, whereby M. rileyi caused significant insecticidal activity on S. litura in 10 days (Dutta et al., 2014). Acid Phosphatase activity (A), Alkaline Phosphatase activity (B), CAT assay (C), Superoxide dismutase activities (D). According to the Tukey test at p = 0.05, statistical values followed by the same letter do not differ significantly (one-way ANOVA).
Frontiers in Microbiology 09 frontiersin.org Likewise, Krishnaveni et al. (2016) reports 80% mortality with a grain formulation of M. rileyi against third instar larvae of S. litura in laboratory experiments. Previous research has found that, among the tested lepidopteran insects, B. bassiana and M. anisopliae produce the greatest insecticidal effect against S. frugiperda (Wraight et al., 2010). The fungi conidia of B. bassiana resulted in 96.6% larval mortality when isolated from cadavers of mosquitoes (García-Munguía et al., 2011). B. bassiana strains, isolated from dead larvae of S. frugiperda caused 74% larval mortality (Cruz-Avalos et al., 2019). Likewise, this present study showed that entomopathogenic fungi M. majus conidia caused high larvicidal activity against S. frugiperda within 9 days under laboratory conditions. Similarly, M. anisopliae and B. bassiana spores display insecticidal activity against disease-transmitting mosquito Aedes and Culex species (Ravindran et al., 2015;Lee et al., 2019;Hamama et al., 2022).
Enzymatic analysis revealed altered ACP, ALP, catalase, and SOD enzyme activity in third instar larvae of S. frugiperda infected with M. majus, thus indicating that the entomopathogenic fungi alters the insect immune system to enable infection. Enzyme levels changed with dose-dependent activity in response to entomopathogenic fungi conidia treatment compared to a control Here, larval ACP, ALP, and catalase enzyme levels significantly decreased following fungi conidia treatment. In contrast, SOD enzyme levels significantly increased following fungi conidia treatment. Catalase (CAT) enzymes are critical in the defence against H 2 O 2 entomopathogens and other toxicants. The findings indicate that the pathogenicity of M. majus fungi conidia reduce the level of CAT enzyme in S. frugiperda larvae. CAT enzymes are also important in the fight against oxidative stress. Wang and Granados (2000) discovered that when S. litura larvae are exposed to UV light, their CAT enzyme levels decrease. The excess ROS produced by the crude extract suppresses antioxidant enzymes. Extremely high levels of H 2 O 2 have been shown to inhibit SOD enzymes by generating more hydroxyl radicals (Vivekanandhan et al., 2022b).
In the current study, M. majus conidia had no toxic effect and caused no mortality in the terrestrial soil bioindicator E. eugeniae following 12 days of treatment. This supports previous research which has reported that entomopathogenic fungi conidia and their secondary metabolites do not kill earthworms (Vivekanandhan et al., , 2022a. The histopathological evaluation of E. eugeniae after 12 days of treatment with M. majus fungal conidia (2.5 × 10 7 conidia/kg) showed that fungal conidia do not damage gut tissues, including lumen and epithelial cells, the nucleus, setae, coelom, mitochondria, and muscles, and that entomopathogenic fungi are unable to penetrate the earthworm epidermis. It is thought that the epidermal mucus of the earthworm E. fetida is capable of destroying the entomopathogenic fungi B. bassiana conidia, thus preventing infection (Zhou et al., 2021). It is possible that the epidermal mucus is also capable of killing M. majus, thus preventing infection of the earthworm as observed in the current study.

Conclusion
In conclusion, M. majus conidia caused high insecticidal efficacy against third instar larvae of S. frugiperda in 2.5 × 10 7 conidia/ml concentration, producing 100% mortality within 9 days. No sublethal toxicity was observed in E. eugeniae after 12 days of treatment. Histopathological evaluation of E. eugeniae exposed to M. majus conidia showed no toxicity effects at 12 days post-exposure. M. majus exposure causes a physiological response in S. frugiperda that results in lower levels of ALP and ACP, CAT enzymes in larvae. Furthermore, after 3 days of treatment, the level of the SOD enzyme increases. The findings suggest that M. majus fungal infection of S. frugiperda larvae affects insect immune function directly, with larval immune function being significantly reduced. Larvicidal activity indicates that M. majus fungi spores can kill S. frugiperda larvae in 3 days. Overall, our findings indicate that M. majus conidia has potential in the control of the agricultural pest S. frugiperda while being less toxic to the non-target organism E. eugeniae.

Data availability statement
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/supplementary material.

Author contributions
VP, PK, and SK: conceptualization. VP and SK: data observation, methodology, and writing -original draft. VP: formal analysis and supervision. VP and PK: investigation. SK: resources and visualization. LA, DE, RM, VP, and SK: validation. VP, LA, DE, RM, SP, RG, PK, and SK: writing -review and editing. All authors contributed to the article and approved the submitted version.

Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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