Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China

Extended-spectrum beta-lactamase-producing Gram-negative bacteria are common in the community and hospitals. To monitor ESBLs mediated by the CTX-M genotype, we collected clinical ESBL pathogenic strains from a hospital in central China and observed a strain of Escherichia coli, namely Ec15103 carrying blaCTX–M–14, blaCTX–M–64 and blaTEM–1, isolated from the blood of a 7-day-old infant in 2015. Strain Ec15103 contains two drug resistance plasmids: pEc15103A, an IncFI-type plasmid that cannot be conjugatively transferred and carries the drug resistance genes blaTEM–1, aacC2, aadA5, sul1, mph(A), sul2, strAB, and tetA(A); and pEc15103B, an IncK2/Z-type plasmid that carries the conjugation transfer gene and blaCTX–M–14. In addition, blaCTX–M–64 is located on the chromosome of Ec15103, and it is the first report of pathogen with blaCTX–M–64 located on its chromosome (the search terms used “blaCTX-M-64” and “chromosome”). blaCTX–M–14 and blaCTX–M–64 are carried by ISEcp1-mediated transposon Tn6503a and Tn6502, respectively. The conjugation transfer ability of pEc15103B was significantly inhibited by zidovudine (AZT) and linoleic acid (LA) and that expression of blaCTX–M–14, blaCTX–M–64 and blaTEM–1 at the mRNA level did not change based on the concentration of cefotaxime or ampicillin. Co-occurrence of blaCTX–M–14 and blaCTX–M–64 in a single isolate will enhance the drug resistance of bacteria, and the presence of blaCTX–M–64 in the chromosome may make the resistance more maintain. This fact will facilitate its dissemination and persistence under different antimicrobial selection pressures. It is essential to prevent these strains from further spreading in a hospital environment.


Introduction
Refractory infections caused by multidrug-resistant (MDR) bacteria are rapidly increasing worldwide, especially for β-lactamase-producing Gram-negative bacteria (Hussain et al., 2021).These Gram-negative bacteria hydrolyze β-lactams, which renders them resistant to β-lactam antibiotics.According to Ambler's classification, β-lactamases can be divided into four categories (Ambler et al., 1991): A (serine penicillinases), B Yang et al. 10.3389/fmicb.2023.1158659(metallo-β-lactamases), C (cephalosporinases), and D (oxacillinases).Extended-spectrum beta-lactamases (ESBLs) are primarily plasmid-mediated, hydrolyzing penicillins, cephalosporins, and monocyclic beta-lactams aztreonam but not cephamycins and carbapenem enzymes.ESBLs can be inhibited by β-lactamase inhibitors, mainly of the class A and D enzymes.Previously, the TEM type and SHV type were the most common genotypes of ESBLs, but since the discovery of the CTX-M β-lactamase, this type has rapidly disseminated worldwide and become the most common ESBL genotype (Bonnet, 2004).It is denoted as CTX-M because it is resistant to cefotaxime but sensitive to ceftazidime (Bauernfeind et al., 1990); it is mainly encoded by plasmids that can be conjugated and transferred.The CTX-M genotype is found in 238 species, and subtypes1 are divided into 6 groups according to amino acid heterogeneity: Group 1, Group 2, Group 8, Group 9, Group 25, and KLUC Group (Rossolini et al., 2008).
ESBL-producing Gram-negative bacteria are common in the community and hospitals (Pitout et al., 2005).They cause a relatively high number of clinical infections, which are usually treated with beta-lactamase inhibitors or carbapenems.Therefore, ESBL-producing strains increase the use of carbapenem antibiotics.As this will further accelerate the evolution of bacterial resistance under antibiotic selection pressure (Bevan et al., 2017), monitoring ESBL strains is particularly important.
To monitor ESBLs mediated by the CTX-M genotype, we collected clinical ESBL-producing isolates from a hospital in central China.In this study, Escherichia coli Ec15103, which carried both bla CTX−M−14 and bla CTX−M−64 , was characterized.Moreover, a detailed genetic characterization of pEc15103A and pEc15103B was revealed.bla CTX−M−14 was located on IncK2/Ztype plasmid pEc15103B, however, bla CTX−M−64 was harbored in the chromosome.

Bacterial strains
Escherichia coli Ec15103 was isolated from the blood of an infected 7-day-old infant at a Chinese teaching hospital in 2015.Bacterial species identification was performed using a Bruker MALDI Biotyper (Bruker Daltonics, Bremen, Germany).E. coli Ec600 (LacZ − , Nal R , Rif R ) was used as the recipient bacterium for plasmid conjugation transfer in this study.Competent E. coli EPI300 was used as the recipient bacterium for plasmid chemical transformation.The strains used in this study can be found in Supplementary Table 1.

ESBL confirmatory test
The ESBL confirmation test used the disc diffusion Antibiotic Sensitivity test (Drieux et al., 2008) and refers to the American Clinical and Laboratory Standards Institute 2020 (CLSI) standard (CLSI, 2020).The zone of inhibition is used to determine the susceptibility of bacteria to an antibiotic.Antimicrobial susceptibility disks (Kangtai Biotechnology, Wenzhou, China) containing ceftazidime (30 µg), cefotaxime (30 µg), ceftazidime/clavulanic acid (30 µg/10 µg), or cefotaxime/clavulanic acid (30 µg/10 µg) were placed on Mueller-Hinton plates (MH plates, Autobio, Zhengzhou, China) coated on the surface with a bacterial suspension (0.5 McFarland turbidity).The size of the inhibition zone was measured after 16-18 h of incubation at 35 • C. Measured the inhibition zone around a disk of cephalosporin and around a disk of the same cephalosporin plus clavulanate.Depending on the disk type, a difference of ≥5 mm between the two diameters are considered as indicating ESBL production.

Plasmid conjugation transfer
Plasmid conjugal transfer experiments were carried out with E. coli Ec600 as the recipient and strain Ec15103 as the donor.The recipient and donor bacteria were inoculated in Lysogeny Broth (LB, Solarbio Science & Technology, Beijing, China) and incubated at 37 • C and 200 rpm overnight.A total of 50 µL of the overnight cultured bacterial suspension was inoculated in 5 mL of LB broth at 37 • C and 200 rpm followed by growth to OD 600 ≈1.0, and then 500 µL of donor and recipient bacteria were mixed.The mixture was spotted onto a 1-cm 2 filter membrane placed on LB nutrient agar (Solarbio Science & Technology, Beijing, China).The plates were incubated at 37 • C for 12∼18 h, then the cells were washed from the filter membrane to 1 mL of LB broth.After 1,000fold dilution, 100 µL was spread on LB nutrient agar medium containing 100 µg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 µg/mL rifampicin (Solarbio Science & Technology, Beijing, China).The colonies after overnight culture at 37 • C were transconjugants (Ec15103-Ec600), as verified by PCR.

Plasmid extraction and transformation
Plasmid DNA of E. coli Ec15103 was extracted using a large plasmid extraction kit (Mei5 Biotechnology, Beijing, China), and plasmid transformation was carried out using competent EPI300 cells as the recipient bacteria.First, competent EPI300 cells were taken out from -80 • C and quickly placed on ice.After 5 min, the bacteria had thawed, the plasmid DNA of E. coli Ec15103 was added, and the mixture was allowed to stand on ice for 30 min.Then, the samples were placed in a water bath at 42 • C for 60 s, quickly placed on ice, and allowed to stand for 5 min.Next, 800 µL of LB broth was added to the EP tube and incubated at 37 • C for 60 min at 200 rpm, followed by centrifugation at 6,000 rpm for 5 min to collect the bacteria.Approximately 100 µL of the supernatant was retained to resuspend the bacteria and spread the cells on LB medium containing 100 µg/mL ampicillin.The colonies after overnight culture at 37 • C were transformants (Ec15103-EPI300-1, Ec15103-EPI300-2), as verified by PCR.

Detection of resistance genes by PCR and product sequencing
Main resistance genes (bla CTX−M−1G , bla CTX−M−9G , and bla TEM−1 ) were screened by PCR (for the primers used, see

Antimicrobial susceptibility testing
The minimum inhibitory concentrations (MICs) values of 25 antibiotics (see Table 1) in this study were evaluated by the broth microdilution method and determined according to Clinical and Laboratory Standards Institute 2020 guidelines (CLSI, 2020).

WGS and analysis
Whole-genome DNA of E. coli Ec15103 was extracted with OMEGA D3350 Bacterial DNA Kit (Omega Bio-Tek, Norcross, GA, USA).The E. coli strain Ec15103 genome was sequenced using a PacBio Sequel II and DNBSEQ platform at Beijing Genomics Institute (BGI, Shenzhen, China).Four SMRT cells Zero-Mode Waveguide arrays of sequencing were used with the PacBio platform to generate the subread set.PacBio subreads (length < 1 kb) were removed.The program Canu was used for self-correction.Draft genomic unitigs, which are uncontested groups of fragments, were assembled using Canu, a high-quality corrected circular consensus sequence subread set.To improve the accuracy of the genome sequences, GATK2 was applied for single-base corrections.Genes were predicted with GeneMarkS TM and RAST and further annotated with BLASTP and BLASTN against the UniProt and NR databases.Annotation of mobile elements was based on the databases ISfinder, INTEGRALL, and Tn Number Registry.Gene organization diagrams were drawn with Inkscape version 0.48.
The genome sequence of strain Ec15103 has been submitted to GenBank: the accession number of the Ec15103 chromosome is CP104274; the accession numbers of pEc15103A and pEc15103B are ON324203 and ON324204, respectively.

Inhibition of plasmid conjugation transfer
Since zidovudine (AZT, Solarbio Science & Technology, Beijing, China) and linoleic acid (LA, Solarbio Science & Technology, Beijing, China) have different solubilities, we used two methods for inhibiting plasmid conjugation transfer.E. coli Ec600 was used as the recipient, and strain Ec15103 was used as the donor.(I) (AZT): The recipient and donor bacteria were inoculated in LB broth at 37 • C and 200 rpm overnight.A total of 5 µL of the overnight bacteria was inoculated in 5 mL of LB broth and incubated at 37 • C and 200 rpm to OD 600 ≈1.0, and then 500 µL of donor and recipient bacteria were mixed.A total of 10 µL of the mixture was spotted on nitrocellulose membrane (4 cm 2 size, 0.45 µm pore size) placed on LB nutrient agar medium, and the different groups of LB nutrient agar contained different concentrations of AZT.The plates were incubated at 37 • C for 12∼18 h, then the cells were washed from the filter membrane to 1 mL of LB broth.After 1,000-fold dilution, 100 µL was spread onto LB nutrient agar medium containing 100 µg/mL ampicillin and 750 µg/mL rifampicin.The colony after 24-28 h of culture at 37 • C was considered the transconjugant (Ec15103-Ec600).(II) (LA): Recipient and donor bacteria were inoculated in LB broth and incubated at 37 • C and 200 rpm overnight.A total of 5 µL of the overnight bacterial culture was inoculated in 5 mL of LB broth and incubated at 37 • C and 200 rpm to OD 600 ≈1.0, and then 500 µL of donor and recipient bacteria were mixed.A 10 µL aliquot was added to 5 mL LB broth and cultured overnight at 37 • C and 200 rpm.The different groups of LB broth contained different concentrations of LA, which was dissolved in 30% DMSO (Solarbio Science & Technology, Beijing, China).A total of 100 µl of the bacteria were cultured overnight and diluted 100-fold, and taken 100 µL to spread onto LB nutrient agar medium containing 100 µg/mL ampicillin and 750 µg/mL rifampicin.The colony after 24-28 h of culture at 37 • C was considered the transconjugant (Ec15103-Ec600).All plasmid conjugation transfer inhibition assays were completed using a minimum of three independent experiments, with three biological replicates per experiment.

RNA extraction and mRNA expression assays (quantitative real-time PCR)
RNA was extracted from 2 mL of logarithmic phase culture grown in LB broth (OD 600 ≈1.0) using a kit (Mei5 Biotechnology, Beijing, China).After reverse transcription real-time quantitative PCR with the SYBR Green method (Pfaffl, 2001), a reaction system with a total volume of 20 µL was established.E. coli 16S rRNA was used as a reference gene to observe relative transcription levels of the target genes bla CTX−M−64 , bla CTX−M−14 , and bla TEM−1 in strains Ec15103, Ec15103-Ec600, and Ec15103-EPI300-1 at different concentrations of cefotaxime (CTX, Solarbio Science & Technology, Beijing, China) or ampicillin (AMP, for the primers used, see Supplementary Table 2).No antibiotics was used as the control group.The 2 − Ct method, corrected for different primer efficiencies and reference genes, was employed.All mRNA expression assays were completed with a minimum of three independent experiments, with at least three biological replicates per experiment.

Statistical analyses
Statistical analysis was performed using GraphPad Prism 8 and SPSS software.All data are presented as the mean standard deviation.

Characterization of E. coli Ec15103
For strain Ec15103, the inhibition zone for cefotaxime was almost 6 mm, and that for cefotaxime/clavulanic acid was 26 mm.The inhibition zone for ceftazidime was 15 mm; that for ceftazidime/clavulanic acid was 23 mm (Figure 1A).The inhibition zone diameter was 5 mm larger than without clavulanic acid, indicating that strain Ec15103 produces ESBL.As determined by PCR screening and sequencing of the major plasmid-borne ESBL and carbapenem resistance genes, strain Ec15103 harbored bla CTX−M −14 , bla CTX−M −64 , and bla TEM−1 but not any of the other genes tested (Figure 2).Plasmid conjugation transfer was carried out using Ec15103 as the donor strain and Ec600 (LacZ − , Nal R , Rif R ) as the recipient strain.However, only one resistance marker, bla CTX−M−14, was transferred from strain Ec15103 to Ec600 through conjugation, generating the transconjugant Ec15103-Ec600 (Figure 2).Additionally, we performed an ESBL confirmation test for Ec15103-Ec600, obtaining an inhibition zone for cefotaxime of 13 mm, for cefotaxime/clavulanic acid of 28 mm, for ceftazidime of 23 mm, and for ceftazidime/clavulanic acid of 30 mm (Figure 1B).As the inhibition zone diameter was 5 mm larger with clavulanic acid than without it, strain Ec15103-Ec600 also shows ESBL enzymatic activity.Both Ec15103 and Ec15103-Ec600 were found to be resistant to ampicillin, ampicillin/sulbactam, cefazolin, cefuroxime, ceftriaxone, and cefotaxime but susceptible to cefoxitin, piperacillin/tazobactam, cefoperazone/sulbactam, meropenem, imipenem, amikacin, polymyxin B, nitrofurantoin, minocycline, and chloramphenicol.Ec15103 was resistant to ceftazidime, cefepime, gentamicin, ciprofloxacin, and cotrimoxazole, whereas Ec15103-Ec600 was not (Table 1).

Whole-genome sequencing and bioinformatics analysis of strain Ec15103
Whole-genome sequencing (WGS) of strain Ec15103 produced a 4991.97-kbcircular chromosome and two plasmids: pEc15103A and pEc15103B.The chromosome has a mean G+C content of 50.6% and consists of 5,121 open reading frames (ORFs).Plasmid pEc15103A is 104.00 kb in length, with an average G+C content of 52.1%, and contains 119 ORFs (Figure 3A).The pEc15103B genome consists of an 87.62-kb circular DNA molecule with an average G+C content of 52.6% and a total of 96 ORFs (Figure 4A).Each plasmid can be divided into backbone and accessory modules.The pEc15103A backbone (Figure 3A) is composed of DNA regions for plasmid replication (repB and repE) and plasmid maintenance (parA, parB, yubM, ssb, etc.).pEc15103A contains five distinct accessory modules (Figure 3A): the MDR region, IS1294-IS2 region, ISKpn26-IS1203 region, IS1A, and IS640.The drug resistance genes are all located in the MDR region, including bla TEM−1 , aacC2, aadA5, sul1, mph(A), sul2, strAB, and tetA(A).
The MDR region of p1-09-02E DNA and pEc15103A are similar, and there is only one modular difference: the 4.7-kb 5 -terminal structure of the MDR region of pEc15103A is lost in p1-09-02E DNA, meanwhile, a unique structure ( Tn6503c-IS26-Tn3 remnant-IS1R) is added in the 3 -end of MDR region in p1-09-02E DNA.Tn6503c is an ISEcp1-mediated transposon and displays an ISEcp1-bla CTX−M−27 -IS903D-iroN structure Inhibition of plasmid conjugation transfer experiments.The effect of drugs on plasmid conjugation transfer was evaluated by comparing the monoclonal colony count of conjugants under different drug concentrations.The LB group was the blank control group, and LA was dissolved in 30% DMSO.(A) The effect of zidovudine (AZT) on plasmid conjugation transfer; AZT was dissolved in water.(B) Effects of linoleic acid (LA) on the conjugative transfer of plasmids; LA was dissolved in 30% DMSO.Data show the mean standard deviation from a minimum of three independent experiments, each with a minimum of three biological replicates.*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, statistical method using one-way ANOVA.(Cheng et al., 2019).For p1-09-02E DNA, Tn6503c is truncated by two copies of IS26, and the line structure Tn6503c is ISEcp1bla CTX−M−27 -IS903D.
pEc15103B is an IncK2/Z plasmid.In this study, the IncK2/Z reference plasmid pO26-CRL-125 (Venturini et al., 2013) (GenBank accession number KC340960) was included in the comparative genomic analysis (Figure 4B).The results showed that pEc15103B contains the conserved IncK2/Z-type plasmid backbone region.There were two accessory regions in pEc15103B, that was IS26 and Tn6503a.The bla CTX−M−14 is located in Tn6503a (Figure 4C).Tn6503a is almost identical to Tn6503c except that bla CTX−M−27 mutates into bla CTX−M−14 .The 3 end of Tn6503a is truncated in pEc15103B, mainly manifesting as deletion of iroN and truncation of IS903D.
In addition, the chromosome of strain Ec15103 contains the drug resistance gene bla CTX−M−64 , which is located in Tn6502 (Figure 4D).The structure of Tn6502 is ISEcp1-bla CTX−M−3 -orf477; in the Ec15103 chromosome, orf477 in this transposon is truncated, and bla CTX−M−3 has undergone mutation of individual bases, forming the structure ISEcp1-bla CTX−M−64 -orf477.

Inhibition of plasmid conjugation transfer
Currently, plasmid-mediated intraspecific and interspecific horizontal gene transfer accounts for the majority of the prevalence and spread of antibiotic resistance genes (Alekshun and Levy, 2007;Carattoli, 2013).However, it has been found that some drugs can inhibit plasmid conjugation transfer (Liu et al., 2020).Using strain Ec15103 as the donor strain and Ec600 as the recipient strain, we performed inhibition of plasmid conjugation transfer experiments as described by Buckner et al. (2020).Compared with the LB broth control group, the number of monoclonal clones of the conjugative transfer strain in the AZT drug group decreased in a concentration-dependent manner, even when the concentration was as low as 0.008 µg/mL, the number of monoclonal clones of the conjugative transfer strain was significantly reduced (P < 0.01; Figure 5A).When the concentration was 0.064 µg/mL, the number of monoclonal clones of the conjugative transfer strain was close to 0, which was very significant (P < 0.0001; Figure 5A).Because LA is insoluble in water, we used 30% DMSO as the solvent, and the results between the 30% DMSO group and the LB broth group were not significantly different (P > 0.05; Figure 5B).When the concentration of LA was 1 mM, the number of monoclonal clones of the conjugative transfer strain was significantly reduced (P < 0.001; Figure 5B), and the number of monoclonal clones of the conjugative transfer strain was further reduced when the concentration of LA was 2 mM, 3 mM, and 6 mM (P < 0.0001; Figure 5B), with a significant difference compared with the 1 mM group (P < 0.01; Figure 5B).

Effect of antibiotics on drug resistance gene expression at the mRNA level
To investigate the effect of antibiotics on expression of resistance genes at the mRNA level, different concentrations of antibiotics were used, and expression of resistance genes was determined by RT-qPCR.Analysis of bla CTX−M−14 and bla CTX−M−64 mRNA levels of strain Ec15103 at different concentrations of CTX showed that expression of bla CTX−M−14 and bla CTX−M−64 did not differ significantly in the presence of CTX (Figures 6A, B).Similarly, expression of bla CTX−M−14 did not change substantially in strain Ec15103-Ec600 in the presence of CTX (Figure 6C), nor did bla TEM−1 mRNA levels in strain Ec15103-EPI300-1 at different concentrations of AMP (Figure 6D).

Discussion
In this study, E. coli Ec15103 was isolated from the blood of a 7-day-old infant in 2015, and Ec15103 harbored two CTX-M genes: bla CTX−M−14 and bla CTX−M−64 .Genome sequencing denoted two drug resistance plasmids: pEc15103A is an IncFI-type plasmid that cannot be conjugatively transferred and contains the drug resistance genes bla TEM−1 , aacC2, aadA5, sul1, mph(A), sul2, Frontiers in Microbiology 08 frontiersin.orgstrAB, and tetA(A); pEc15103B contains the conjugation transfer gene, is an IncK2/Z-type plasmid and contains bla CTX−M−14 .In pEc15103B, bla CTX−M−14 is located in Tn6503a with a structure of ISEcp1-bla CTX−M−14 -IS903D.bla CTX−M−64 was located on the chromosome of strain Ec15103, and it is the first report of pathogen with bla CTX−M−64 located on its chromosome (the search terms used "blaCTX-M-64" and "chromosome").
In plasmid conjugation transfer inhibition experiments, the conjugation transfer ability of plasmid pEc15103B was significantly inhibited by AZT and LA, which is consistent with the results of Buckner et al. (2020).In general, conjugative transfer of plasmids contributes significantly to the spread of drug resistance genes (Grohmann et al., 2003;Harrison and Brockhurst, 2012).In the post-antibiotic era, it seems reasonable to curb the spread of drug resistance genes and slow the pace of superbug production by inhibiting plasmid conjugation transfer, as the human intestine is a natural library for drug resistance gene spread (Huddleston, 2014).AZT was the first antiretroviral agent to be licensed for treating human immunodeficiency virus (HIV) infection (Rachlis, 1990), and 0.008 µg/mL is much lower than the dose of the drug in AIDS treatment.However, it can significantly inhibit the conjugative transfer of the plasmid.LA, which is an essential nutrient (Whelan and Fritsche, 2013), is very effective in inhibiting the conjugation and transfer of plasmids, and is an excellent choice.Nevertheless, these drugs may not be effective for the treatment of infection and will increase the patient's treatment burden, and there is no animal study on their inhibitory effect.
In addition, we sought to determine whether the expression of these resistance genes increases with an increase in antibiotic concentration.Therefore, we conducted qPCR experiments to verify the relationship between expression of drug-resistance genes and the concentration of antibiotics at the mRNA level.Under the conditions of different concentrations of cefotaxime, mRNA expression levels of the genes bla CTX−M−14 and bla CTX−M−64 in strains Ec15103 and Ec15103-Ec600 did not change significantly.Similarly, under the effect of ampicillin, expression at the mRNA level of the bla TEM−1 gene of strain Ec15103-EPI300-1 was also unaffected.This is different from the description by Kjeldsen et al. (2015), probably because of differences between strains, and some studies had noted that relative expression at the mRNA level and the protein level of the bla CTX−M gene differ (Geyer et al., 2016).
Due to the presence of bla CTX−M−64 on the chromosome, strain Ec15103 exhibited resistant to ceftazidime; conversely, other conjugated and transformed strains were sensitive.bla CTX−M−64 was first reported in 2009 and found on a Shigella drug resistance plasmid in Japanese patients with diarrhea after travel to China (Nagano et al., 2009).It is considered to be a hybrid chimera of bla CTX−M−15 (CTX-M-1G) and bla CTX−M−14 (CTX-M-9G) and shows strong ceftazidime hydrolysis activity.There were relatively few subsequent reports of pathogenic bacteria carrying Frontiers in Microbiology 09 frontiersin.orgthe bla CTX−M−64 resistance gene, and in most cases, it was found on plasmids.At present, it is generally believed that the CTX-M gene derive from the chromosome of Kluyvera spp (Poirel et al., 2002) and that it became incorporated on a plasmid through the action of mobile elements such as transposons and integrons.Therefore, it is generally believed that the CTX-M resistance genes in other bacteria are mainly located on plasmids (D'Andrea et al., 2013).However, there are an increasing number of reports of chromosomally located CTX-M genes (most of which are bla CTX−M−15 and bla CTX−M−14 ) recently (Irrgang et al., 2017;Zheng et al., 2021).Therefore, as the CTX-M drug resistance gene derives from the chromosome, it may again be inserted into the bacterial chromosome.In addition to E. coli, Klebsiella pneumoniae (Coelho et al., 2010), Acinetobacter baumannii (Potron et al., 2011), Proteus mirabilis (Song et al., 2011), and Salmonella (Zhang et al., 2019) all harbor CTX-M gene on the chromosome.This also demonstrates that insertion of bla CTX−M into the chromosome by a plasmid under the action of a transposon is not a random event.Indeed, bacterial resistance may be evolving in this direction.
Because the presence of plasmids is not without cost to bacteria, those containing plasmids often have a relatively long replication cycle (Harrison and Brockhurst, 2012), and the plasmids may be lost during passage and reproduction.Once the resistance gene is inserted into the chromosome, there is no longer a cost, and the resistance gene will continue to replicate with each division cycle, presenting another challenge for antibiotic treatment.

FIGURE 1 ESBL
FIGURE 1 ESBL confirmatory test.(A) The ESBL confirmation test of Ec15103.(B) The ESBL confirmation test of Ec15103-Ec600.Each experiment was conducted with three parallel replicates, and only one representative figure is shown.

FIGURE 2
FIGURE 2Detection of drug resistance genes by PCR.

FIGURE 3 General
FIGURE 3 General Features of pEc15103A.(A) Schematic map of the sequence of plasmid pEc15103A.Genes are denoted by arrows and colored according to gene function classification.The innermost circle presents the GC skew [(G-C)/(G+C)] with a window size of 500 bp and a step size of 20 bp.The next-to-innermost circle presents the GC content.The backbone and accessory module regions are also shown.(B) Linear comparison of the sequenced plasmid pEc15103A.Genes are denoted by arrows colored according to gene function classification.Shaded regions denote regions of homology (>95% nucleotide identity).(C) Linear comparison of bla TEM−1 genetic surroundings.Genes are denoted by arrows and colored based on gene function classification.

FIGURE 4
FIGURE 4 General features of pEc15103B and bla CTX−M−64 .(A) Schematic map of the sequence of plasmid pEc15103B.Genes are denoted by arrows and colored according to gene function classification.The innermost circle presents the GC skew [(G-C)/(G+C)] with a window size of 500 bp and a step size of 20 bp.The next-to-innermost circle presents the GC content.The backbone and accessory module regions are also shown.(B) Linear comparison of the sequenced plasmid pEc15103B.Genes are denoted by arrows colored according to gene function classification.Shaded regions denote regions of homology (>95% nucleotide identity).(C) Linear comparison of bla CTX−M−14 genetic surroundings.Genes are denoted by arrows and colored based on gene function classification.(D) Linear comparison of bla CTX−M−64 genetic surroundings.Genes are denoted by arrows and colored based on gene function classification.

FIGURE 6
FIGURE 6Relative changes in resistance gene mRNA levels in strains Ec15103, Ec15103-Ec600, and Ec15103-EPI300-1 under the influence of antibiotics.(A,B) Relative changes in bla CTX−M−14 and bla CTX−M−64 mRNA levels in strain Ec15103 under different concentrations of cefotaxime (CTX); bla CTX−M−14 is present on the Ec15103 plasmid pEc15103B and bla CTX−M−64 on the Ec15103 chromosome.(C) Relative changes in bla CTX−M−14 mRNA levels in strain Ec15103-Ec600 at different concentrations of CTX.bla CTX−M−14 is located on the Ec15103-Ec600 conjugate plasmid pEc15103B.(D) Relative changes in bla TEM−1 mRNA levels in strain Ec15103-EPI300-1 at different concentrations of ampicillin (AMP).bla TEM−1 is located on the Ec15103-EPI300-1 transconjugant plasmid pEc15103A.For at least two independent experiments, each with at least three biological replicates, the data shown represent the mean standard deviation using one-way ANOVA.

TABLE 1
Mics of antibiotics and compounds.