The occurrence and molecular detection of mcr-1 and mcr-5 genes in Enterobacteriaceae isolated from poultry and poultry meats in Malaysia

The advent of antimicrobials-resistant (AMR), including colistin-resistant bacteria, poses a significant challenge to animal and human health, food safety, socio-economic growth, and the global environment. This study aimed to ascertain the colistin resistance prevalence and molecular mechanisms of colistin resistance in Enterobacteriaceae. The colistin resistance was determined using broth microdilution assay, PCR; and Sanger sequencing of mcr genes responsible for colistin resistance in Enterobacteriaceae (n = 627), including Escherichia coli (436), Salmonella spp. (n = 140), and Klebsiella pneumoniae (n = 51), obtained from chicken and chicken meats. Out of 627 Enterobacteriaceae, 8.6% of isolates exhibited colistin resistance phenotypically. Among these colistin resistant isolates, 9.3% (n = 37) were isolated from chicken meat, 7.2% (n = 11) from the cloacal swab of chicken and 7.9% (n = 6) from the litter samples. Overall, 12.96% of colistin-resistant isolates were positive with mcr genes, in which mcr-1 and mcr-5 genes were determined in 11.11% and 1.85% of colistin-resistant isolates, respectively. The E. coli isolates obtained from chicken meats, cloacal swabs and litter samples were found positive for mcr-1, and Salmonella spp. originated from the chicken meat sample was observed with mcr-5, whereas no mcr genes were observed in K. pneumoniae strains isolated from any of the collected samples. The other colistin resistance genes, including mcr-2, mcr-3, mcr-4, mcr-6, mcr-7, mcr-8, mcr-9, and mcr-10 were not detected in the studied samples. The mcr-1 and mcr-5 genes were sequenced and found to be 100% identical to the mcr-1 and mcr-5 gene sequences available in the NCBI database. This is the first report of colistin resistance mcr-5 gene in Malaysia which could portend the emergence of mcr-5 harboring bacterial strains for infection. Further studies are needed to characterize the mr-5 harbouring bacteria for the determination of plasmid associated with mcr-5 gene.

The advent of antimicrobials-resistant (AMR), including colistin-resistant bacteria, poses a significant challenge to animal and human health, food safety, socioeconomic growth, and the global environment. This study aimed to ascertain the colistin resistance prevalence and molecular mechanisms of colistin resistance in Enterobacteriaceae. The colistin resistance was determined using broth microdilution assay, PCR; and Sanger sequencing of mcr genes responsible for colistin resistance in Enterobacteriaceae (n = 627), including Escherichia coli (436), Salmonella spp. (n = 140), and Klebsiella pneumoniae (n = 51), obtained from chicken and chicken meats. Out of 627 Enterobacteriaceae, 8.6% of isolates exhibited colistin resistance phenotypically. Among these colistin resistant isolates, 9.3% (n = 37) were isolated from chicken meat, 7.2% (n = 11) from the cloacal swab of chicken and 7.9% (n = 6) from the litter samples. Overall, 12.96% of colistin-resistant isolates were positive with mcr genes, in which mcr-1 and mcr-5 genes were determined in 11.11% and 1.85% of colistin-resistant isolates, respectively. The E. coli isolates obtained from chicken meats, cloacal swabs and litter samples were found positive for mcr-1, and Salmonella spp. originated from the chicken meat sample was observed with mcr-5, whereas no mcr genes were observed in K. pneumoniae strains isolated from any of the collected samples. The other colistin resistance genes, including mcr-2, mcr-3, mcr-4, mcr-6, mcr-7, mcr-8, mcr-9, and mcr-10 were not detected in the studied samples. The mcr-1 and mcr-5 genes were sequenced and found to be 100% identical to the mcr-1 and mcr-5 gene sequences available in the NCBI database. This is the first report of colistin resistance mcr-5 gene in Malaysia which could portend the emergence of mcr-5 harboring bacterial strains for infection. Further studies are needed to characterize the mr-5 harbouring bacteria for the determination of plasmid associated with mcr-5 gene.

Introduction
Antimicrobial agents are essential medicines in animals and humans to curb infections. Owing to the overuse and abuse of antimicrobial agents, the globe is being faced with the rapid proliferation of resistant microbes. Currently, the advent of antimicrobial-resistant (AMR) bacteria poses a significant challenge to animal and human health, food safety, socio-economic growth, and the global environment (Theuretzbacher, 2017). The proliferation of Gram-negative bacterial strains which are resistant to multiple drugs, and the absence of new drugs to combat such microbes, reintroduced colistin as a last-line therapy (Ezadi et al., 2019).
The resistance to colistin is a crucial problem to be tackled today. Numerous studies have demonstrated that this colistin resistance was present in various bacterial strains around the world. The plasmidmediated mcr genes are accountable for exceptional colistin resistance, as it is a conduit that spreads via horizontal transmission from one bacterial strain to another and through food chain or direct contact to humans, animals, and the environments (Gharaibeh and Shatnawi, 2019). Before 2015, all documented colistin-resistance was chromosomally regulated, involving modification of a two-component regulatory structure, phoPQ and pmrAB with the negative regulator, mgrB gene alteration (Cannatelli et al., 2013;Liu et al., 2016). In 2016, the Escherichia coli strains isolated from humans, retail chicken meat and pork, and Klebsiella pneumoniae strains isolated from humans were reported with the plasmid-encoded mcr-1 gene in China (Liu et al., 2016). The plasmid-mediated new mcr genes have rapidly emerged. The therapeutic effectiveness of colistin has been compromised by the advent of the plasmid-encoded mcr genes, including mcr-1 to mcr-10, which were reported during the last four years (Ling et al., 2020). The E. coli isolates recovered from chicken liver, and chicken feed in the trough (Yu et al., 2016), and poultry meat in Malaysia were found positive for mcr-1 gene (Aklilu and Raman, 2020).
Poultry meat is an important source of protein for humans, it could also be a significant conduit for spreading multidrug-resistant bacterial species from food-producing animals to humans. Previous study has shown that retail chicken meat plays a role in disseminating multiple antibiotic-resistant strains among humans and their environment, posing a severe threat to environmental health and food safety (Aidara-Kane et al., 2013). The colistin resistance-producing gene, mcr-1, was present in 52.1 percent of the E. coli isolates from raw chicken meat (Aklilu and Raman, 2020). Colistin resistance was found in more in 36.4% of bacteria from poultry chicken and 20% of strains isolated from native chicken in Bangladesh (Islam et al., 2020). In Nepal, it was reported that 27 (22.8%) of colistin-resistant E. coli in broiler farms carried the mcr-1 gene (Joshi et al., 2019). Aside from that, the horizontal transmission is thought to be the main mechanism for the spread of colistin resistance mcr genes in Enterobacteriaceae worldwide (Gharaibeh and Shatnawi, 2019).
With potent in vitro transfer rates and frequently harboured alongside other resistance determinants like β -lactamases, mcr genes have been identified on a variety of conjugative plasmids (IncI2, IncHI2, IncX4, and pHNSHP45) (EMA (European Medicine Agencey), 2016). As the mobilized colistin resistance (mcr) genes driven by plasmids are quickly emerging, appropriate information on colistin resistance, including the incidence and epidemiological studies of mcr-positive cases, is required to apply steps to prevent and manage its dissemination. Therefore, the aim of the study was to determine the prevalence and molecular determinants underlying colistin resistance in Enterobacteriaceae (E. coli, Salmonella spp., and Klebsiella pneumoniae) isolates recovered from poultry and poultry meats in Malaysia.

Ethics approval
The ethical board of Universiti Putra Malaysia (UPM), Institutional Animal Care and Use Committee (IACUC) approved the research study protocol for collecting cloacal swabs from live poultry (UPM/IACUC/AUP-R091/2019).

Study design and samples
The research study was performed in which chicken meat samples from supermarkets and cloacal swabs, and litter samples from chicken farms within Selangor, Malaysia (Figure 1) were collected from July 2019 to February 2021. Selangor is the densely populated area in Malaysia and most of the poultry farms are located in this state. Sterile plastic bags were used to collect the meat samples. The cloacal swab samples were collected aseptically from the healthy chicken and kept in sterile transport media, Stuart media. Litter samples were collected from the farms' floors using a sterile spoon, and placed in a sterile plastic bag. In total, 543 samples, including 350 chicken meats, 144 cloacal swabs, and 49 litter samples (Table 1), were collected from supermarkets and poultry farms in different areas of Selangor in Malaysia ( Figure 1). All collected samples were immediately transported in a sealed icebox to the Bacteriology Laboratory, Faculty of Veterinary Medicine at Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia.

Isolation and identification of bacterial strains
In the Enterobacteriaceae family, Salmonella spp., E. coli, and K. pneumoniae have public health importance and are more prevalent with colistin resistance mcr genes (Elbediwi et al., 2019). For this reason, Salmonella spp., E. coli, and K. pneumoniae, were isolated and identified phenotypically using the standard protocol of traditional cultural and biochemical tests (Ghafur et al., 2019;Sharma et al., 2019). In the pre-enrichment step, the samples were cultured in buffered peptone water (BPW, Oxoid, United Kingdom), including 25 g of meat in 225 mL, 10 g litter in 90 mL, and cloacal swabs in 10 mL BPW medium, samples were incubated at 37°C for 24 h. The meat samples with BPW were homogenized for 2 min in a stomacher. For Salmonella spp. isolation, 100 μL of homogenized BPW was mixed with 10 mL of Rappaport-Vassiliadis (RVS; Oxoid, United Kingdom). Then the RVS mixtures were incubated at 42°C for 24 h. One loop-full RVS of each sample was sub-cultured onto Xylose Lysine Deoxycholate agar (XLD; Oxoid, United Kingdom) and kept at 37°C for 24 h for incubation. On XLD, typically, Salmonella colonies were red with a black centre. One pure colony from XLD was cultured onto nutrient agar (NA, Oxoid,

Molecular confirmation of Escherichia coli, Salmonella spp., and Klebsiella pneumoniae
The genomic DNA was extracted from the pure culture of the isolates cultured on nutrient agar using the boiling and snap chill method (Pui et al., 2011). The phenotypically positive E. coli, Salmonella spp., and Klebsiella pneumoniae species were then confirmed with conventional PCR with species-specific gene primers (Table 1), in which positive controls included E. coli ATCC 25922, Salmonella ATCC 14028 and Klebsiella pneumoniae ATCC 700603 (Rahn et al., 1992;Ranjbar et al., 2016;Moawad et al., 2018).

Colistin-susceptibility and minimum inhibitory concentration (MIC) assessment
Colistin-susceptibility and MIC in isolates were assessed by the ISO-20776 standard broth microdilution technique (BMD) jointly recommended by the EUCAST (2016a) with few modifications. In brief, a two-fold dilution (0.125-128 μg/mL) of the colistin sulfate salt (Sigma-Aldrich) was prepared on a 96 well microtitre plate. The bacterial inoculum was inoculated in each well with a final concentration of 5 × 10 5 CFU/mL. Then the microtitre plate was incubated at 37°C for 16-20 h. After the incubation period, 30 μL of 0.015% resazurin solution Map showing the sampling areas of Selangor, Malaysia. Star (*) and triangle (▲) marks indicating supermarkets and poultry farms, respectively. The geographic information software, ArcGIS v.10.4.1 for Windows was used to generate the sampling spot on the map.

Detection of the colistin resistance determinants, mcr genes
Colistin-resistant Enterobacteriaceae isolates were identified with MIC values greater than 2 μg/mL colistin (EUCAST, 2016b). The genomic DNA of the colistin-resistant (col-R) isolates was assessed with conventional PCR to detect colistin resistance (mcr) gene variants (mcr-1 to mcr-10). According to previous studies, the detection of mcr-1 to mcr-5 (Rebelo et al., 2018) and mcr-6 to mcr-9 (Borowiak et al., 2020) was performed with multiplex PCR. The uniplex PCR was performed for mcr-10 (Xu et al., 2021) by the previously designed oligonucleotide primers and the protocol (Table 2).

PCR results analysis
Amplified PCR products were examined on 1.5% agarose (Conda, Madrid, Spain) prepared in 100 mL of 0.5 × TBE Buffer stained with 4 μL of Nucleic Acid stain (ETB "out" Nucleic Acid, Cat. No. FYD007-200P, Yestern Biotech Co. ltd, Taiwan) for mcr gene. The expected bands for mcr-1 to mcr-10 ( Confirmation of mcr (mcr-1 and mcr-5) gene by sequencing The amplified PCR products for the mcr-1 gene of E. coli strains, E. coli E172, and mcr-5 gene from one Salmonella spp. strain S283 were sent to commercial company for DNA sequencing with the same primers for Sanger sequencing (Table 2) and compared to previously reported mcr-1 and mcr-5 sequences in the NCBI database. The consensus sequences of mcr-1 and mcr-5 were obtained based on the alignment of the forward and reverse sequences using BioEdit v. 7.2 program (Hall, 1999).

Phylogenetic analysis
The mcr gene sequences were used in the phylogenetic tree construction, and analysis was carried out according to Li et al. (2019). The sequences from this study and those from GenBank for the mcr-1 and mcr-5 were aligned separately using the MEGA X software (Kumar et al., 2018) to compare their similarities. There are four different types of mcr-5 gene variants such as mcr-5.1, mcr-5.2, mcr-5.3 and mcr-5.4 has been identified in the world. We have selected partial Frontiers in Microbiology 05 frontiersin.org sequences of these four mcr-5 variants from GenBank in the NCBI database and analysed with our mcr-5 sequence data. The phylogenetic tree for mcr-1 and mcr-5 was constructed with aligned sequences by the neighbor-joining method using the Kimura 2-parameter model, and Bootstrap values were calculated using 500 replicates.

Statistical analysis
Microsoft Excel sheets (MS-2019) were used to input data, which were then uploaded into the SPSS program v. 25.0 (IBM, Armonk, NY, United States). Descriptive analysis was used to quantify the prevalence, in which level of significance was assessed using the χ 2 test. For statistical significance, p-values less than 0.05 (p < 0.05) was taken into consideration.

Detection of colistin resistance determinants, mcr gene variants
All 54 isolated colistin-resistant (Col-R) Enterobacteriaceae were analyzed to observe the presence of mcr-1 to mcr-10. Overall, 12.96% (n = 7) of colistin-resistant Enterobacteriaceae isolates were found possessing colistin resistance mcr genes comprising 8.1% (n = 3), 27.3% (n = 3), and 16.67% (n = 1) of Col-R isolates from the chicken meat, chicken and litter samples, respectively. Variations of colistin resistance from different sources were not statistically significant (value of p > 0.05) as shown in Table 4. Out of seven mcr harboring Col-R isolates, 11.11% (n = 6) and 1.85% (n = 1) were found with mcr-1 and mcr-5, respectively. The E. coli isolates obtained from chicken meats, cloacal swabs and litter samples were found positive for mcr-1, and Salmonella spp. originated from the chicken meat sample was observed with mcr-5, whereas no mcr genes were observed in K. pneumoniae strains isolated from any of the collected samples ( Figure 3). The other colistin resistance genes, including mcr-2, mcr-3, mcr-4, mcr-6, mcr-7, mcr-8, mcr-9, and mcr-10 were not detected in the studied samples.

Confirmation of mcr gene variants
In BLAST analysis, our studied ¬mcr-1 sequence was found to be 100% identical to the mcr-1 sequence (Genbank: NG_050417.1) in the NCBI database with 99% query coverage. On the other hand, our studied ¬mcr-5 sequence was found to be 100% identical to the mcr-5 sequence (Genbank: NG_055658) in the NCBI database with 98% query coverage.

Minimum inhibitory concentration determination
The broth microdilution test was conducted to assess the MIC value of all isolates following EUCAST guidelines, with the epidemiological cutoff value >2 μg/mL for colistin resistance. Control strains, colistin-resistant isolates Escherichia coli NTCC 13846 and susceptible isolate Escherichia coli ATCC 25922, showed growth up to 4 μg/mL and 0.5 μg/mL colistin concentration, respectively. The MIC value of isolated colistin-resistant bacteria exhibited 4 to 128 μg/mL of colistin. The mcr-carrying isolates were observed with MIC values of 4 and 8 μg/mL colistin (Figure 4). In contrast, mcr negative colistin-resistant isolates had extremely high   Figure S1).

Phylogenetic comparison
In the phylogeny analysis, it is observed that mcr-1 genes in the obtained isolates were divided into two clades, named clade A, including subclade I and subclade II, and clade B. The colistin resistance mcr-1 gene sequence from E. coli strains E48, E13, E278, E297, E331 recovered from cloacal swabs and chicken meat samples were clustered in subclade I. These isolates were closely related to subclade II, grouped with E. coli and Salmonella spp. strains recovered from humans and animals obtained from the NCBI GenBank database. The nucleotide sequence of the mcr-1 gene of E. coli E172 obtained from chicken litter samples was very close to the previously identified isolate in Malaysia and human isolate in China. On the other hand, mcr-1 sequences of E. coli isolates recovered from chicken meats, and chicken cloacal swab samples were grouped in one cluster and were found close to isolates from China and Brazil ( Figure 5).
In the phylogeny analysis of the mcr-5 gene, it was revealed that the nucleotide sequence of the mcr-5 gene of Salmonella spp. obtained from chicken meat has a close relation to the mcr-5.3 gene in the E. coli isolate obtained from the horse in Brazil (Figure 6).

Discussion
The most common bacteria associated with bacterial infections in chickens are E. coli, Salmonella spp., and K. pneumoniae. These microorganisms are known to cause serious health issues, which can result in increased mortality, decreased productivity, and higher costs for both disease prevention and treatment (Ibrahim et al., 2021). Escherichia coli isolates were the most prevalent in the collected samples, followed by Salmonella spp. and K. pneumoniae isolates. Escherichia coli isolates were high prevalence in chicken cloacal swabs (93.1%), Salmonella spp. were in chicken meats (34.9%), and K. pneumoniae isolates were in litter samples (36.7%). Previous studies have also reported 83 and 53.04% of cloacal swabs were found positive for E. coli in Malaysia and China, respectively (Suryadevara et al., 2020;Li et al., 2022). In Indonesia, 13.75% of cloacal swabs obtained from broiler chicken farms were found positive for K. pneumoniae (Permatasari et al., 2020). Another study in Malaysia showed that the prevalence of Salmonella spp. and E. coli from cloacal swabs obtained from broilers were 6.5 and 51.8%, respectively (Ibrahim et al., 2021). Backyard chickens in Malaysia were documented with 2.5% of Salmonella spp. (Jajere et al., 2019). In contrast, a much higher prevalence of Salmonella spp. (48%) in chicken meat samples was observed in Bangladesh (Islam et al., 2016). In Nepal, 33.33% of chicken meat samples were positive for E. coli (Joshi et al., 2019) which is lower than our findings, indicating that the frequency of E. coli in chicken meat might vary widely. Different levels of hygienic practices in various geographic regions and environmental factors, such as The rate trend and number of phenotypically colistin-resistant Enterobacteriaceae isolates, E. coli, Salmonella spp., and K. pneumoniae. The blue column = rate trend and red line = number of isolates, left y axis = % of colR isolates, right y axis = number of colR isolates.
Frontiers in Microbiology 07 frontiersin.org exposure to poor sanitation, could be the source of the variations in prevalence (Dawadi et al., 2021). Colistin is being prescribed for the therapeutic purposes of bacterial infection in humans that are resistant to multiple drugs despite its side effects. The introduction of the colistin resistance (mcr) gene in bacterial strains that are already resistant to many antibiotics, creates the ineffectiveness of colistin, the last resort drug. In this study, the Enterobacteriaceae isolates recovered from various sources, comprising chicken meat from supermarkets and cloacal swabs and litter samples from poultry farms, were prevalent with colistin resistance and mcr genes. The MIC value of colistin-resistant isolates ranged from 4 μg/mL to 128 μg/mL of colistin, however, low MIC values were found in mcr positive isolates. In previous studies, the mcr-1 had been documented from Klebsiella pneumoniae, and E. coli isolates in Malaysia (Yu et al., 2016;Mobasseri et al., 2019;Aklilu and Raman, 2020). The colistin resistance mcr-1 gene is also observed in E. coli isolates from poultry in many countries in Asia (Trung et al., 2017;Joshi et al., 2019;Amin et al., 2020). A greater percentage of mcr-1 (>94%) was depicted in bacterial strains from turkey and broiler feces in Germany (Irrgang et al., 2016). The colistin resistance gene (mcr-1) was found in 25.8% of poultry (turkey and chicken) meats from Italy (5 samples) and Germany (28 samples) but not in any samples (turkey and chicken meats) from Switzerland, Denmark, Austria, or Hungary, according to a Swiss study (Zurfluh et al., 2016). In Brazil, 19.5% of chicken meat and liver samples were positive for E. coli harboring mcr-1 (Monte et al., 2017). All the The rate of Enterobacteriaceae isolates harboring mcr gene based on sample sources. The rate of mcr genes was marked by blue, red and green bars to the Y-axis for chicken meat, chicken cloacal swab and litter samples, respectively. Colistin MICs of Enterobacteriaceae with the mcr-genes.
Frontiers in Microbiology 08 frontiersin.org colistin-resistant E. coli obtained from raw beef and beef products in Egypt were found positive for mcr-1 (Sabala et al., 2021). In comparison with previous studies, a low prevalence of mcr-1 gene in colistin-resistant isolates was found in the current research. The explanation for the low incidence in Malaysia isolates is unknown, although other resistance mechanisms, such as chromosomal alteration and modulation in the mgrB gene, could be involved (Chen et al., 2017). The colistin resistance gene, mcr-5 was detected in Salmonella spp. for the first time in Malaysia. There were no reports about this gene in any bacterial strains from animals or humans in Malaysia to the best of our knowledge. In this study, the mcr-5 gene in Salmonella spp. was found at a very low rate. This finding is consistent with a previous report in which 2.5% (8/315) of colistin-resistant Salmonella spp. originated from pigs and meats (pork) in Germany (Borowiak et al., 2019), and 0.7% of human vaginal swab samples in Yangzhou in China (Zhang et al., 2018) were found to be positive for the mcr-5 gene. The mcr-5 gene was also observed in colistin-resistant E. coli strains obtained from cloacal swabs of poultry in Nigeria (Ngbede et al., 2020), veal in Belgium (Timmermans et al., 2021), pork samples in Cambodia (Pungpian et al., 2021) and poultry and pigs samples in Spain and China (García-Meniño et al., 2019). The situation is concerning because these resistant pathogens could be spread to humans through the food chain or close contact with animals (Dawadi et al., 2021).
The mcr-1 gene was found among the isolates showing MIC values of 4 μg/mL and 8 μg/mL of colistin in this study which is consistent with the previous studies in Bangladesh and China, which showed that colistin-resistant isolates with mcr-1 had a MIC value of 4 μg/mL, and 8 to 16 μg/mL of colistin, respectively (Islam et al., 2017;Amin et al., 2020). Phylogenetic tree of mcr-1 gene showing the relationship of the partial coding regions of mcr-1 gene sequences. The studied sequences were marked by red boxes. MEGA X was used to create the phylogenetic tree. Phylogenetic tree inferred from the nucleotide sequences of mcr-5 gene using the maximum likelihood method for distance calculations using MEGA X software. Bootstrap percentages retrieved in 500 replications are shown at the nodes. The studied sequence is marked by the red box.
Frontiers in Microbiology 09 frontiersin.org In nucleotide BLAST, the sequences of the mcr-1 gene isolated from E. coli and the mcr-5 gene obtained from Salmonella spp. were found to be 100% identical to previously reported mcr-1 and mcr-5 gene sequences in the NCBI database. In the phylogenetic analysis, mcr-1 gene sequences were closely related to previous reports, mcr-1 gene sequence of E. coli isolates recovered from chicken in Malaysia (Yu et al., 2016) and E. coli strain isolated from human in China (Genbank_MZ450868). This suggests that mcr-1 gene in E. coli has been circulating in Malaysia, which is a threat to animals and public health. The colistin resistance mcr-5 gene was detected in Salmonella spp. isolate for the first time in Malaysia. The various types of mcr-5 gene variants, including mcr-5.1 to mcr-5.4, were identified in the world (Fleres et al., 2019;Ling et al., 2020). In the phylogeny, the mcr-5 gene sequence of the current study was closely related to mcr-5.3 gene recovered from E. coli isolates obtained from the horse in Brazil (GenBank database, NG_061405).

Conclusion
The introduction and dissemination of colistin resistance with mcr genes in Enterobacteriaceae is a major worldwide issue. Colistinresistant Enterobacteriaceae were observed in poultry meats and poultry farms in the present study. The mcr-1 and mcr-5 genes were found in colistin-resistant E. coli and Salmonella spp., respectively. The mcr-5 has been identified in Malaysia for the first time, which could signal the advent of mcr-5 harboring bacterial strains for infection. The existence of mcr-positive Enterobacteriaceae in poultry and poultry meat in Malaysia emphasizes the importance of proper poultry waste disposal and good hygiene practices among people who are exposed to poultry and poultry meats.

Data availability statement
The nucleotide sequences of mcr-1 and mcr-5 have been submitted to the GenBank of the NCBI (accession no. OR333822, OR333835). The raw data supporting the findings of this article will be made available by the authors, without undue reservation.

Ethics statement
The animal study was reviewed and approved by the ethical board of Universiti Putra Malaysia (UPM), Institutional Animal Care and Use Committee (IACUC) approved the research study protocol for collecting cloacal swabs from live poultry (UPM/IACUC/AUP-R091/2019).