Comparative proteomics reveals the mechanism of cyclosporine production and mycelial growth in Tolypocladium inflatum affected by different carbon sources

Cyclosporine A (CsA) is a secondary cyclopeptide metabolite produced by Tolypocladium inflatum that is widely used clinically as an immunosuppressant. CsA production and mycelial growth differed when T. inflatum was cultured in different carbon source media. During early fermentation, CsA was preferred to be produced in fructose medium, while the mycelium preferred to accumulate in sucrose medium. On the sixth day, the difference was most pronounced. In this study, high-throughput comparative proteomics methods were applied to analyze differences in protein expression of mycelial samples on day 6, revealing the proteins and mechanisms that positively regulate CsA production related to carbon metabolism. The differences included small molecule acid metabolism, lipid metabolism, organic catabolism, exocrine secretion, CsA substrate Bmt synthesis, and transcriptional regulation processes. The proteins involved in the regulation of mycelial growth related to carbon metabolism were also revealed and were associated with waste reoxidation processes or coenzyme metabolism, small molecule synthesis or metabolism, the stress response, genetic information or epigenetic changes, cell component assembly, cell wall integrity, membrane metabolism, vesicle transport, intramembrane localization, and the regulation of filamentous growth. This study provides a reliable reference for CsA production from high-efficiency fermentation. This study provides key information for obtaining more CsA high-yielding strains through metabolic engineering strategies.


Introduction
The application of cyclosporine A (CsA) is another great discovery in the exploration of secondary fungal metabolites since the discovery of penicillin (Survase et al., 2011;Kjaerbølling et al., 2019).CsA inhibits the mixed lymphocyte response and contrasts with other immunosuppressants and cytostatic drugs with its weak myelotoxicity (Borel et al., 1976).Thus, CsA has been widely used in bone marrow immunotherapy and organ transplantation to inhibit chronic inflammatory reactions, HIV, and hepatitis C virus (Survase et al., 2011).Wang et al. 10.3389/fmicb.2023.1259101Frontiers in Microbiology 02 frontiersin.org CsA is a non-ribosomal peptide (NRP) secondary metabolite mainly derived from the Ascomycota fungus Tolypocladium inflatum W. Gams 1971 (Rossman et al., 2017).Tolypocladium inflatum is widely produced in the pharmaceutical industry but more efficient production methods are needed to optimize the procedure.Current research has focused on obtaining better strains using chemical mutagenesis and optimizing medium using high throughput contrast tests.Tolypocladium strain VCRC F21 NRRL No. 18950 produces a high level of CsA in composition-optimized fermentation media and has been exploited for industrial production (Balaraman and Mathew, 2006).Diethyl sulfate-based mutagenesis was performed on the fungal strain Tolypocladium inflatum MTCC-3538.Liquid chromatography-mass spectrometry (LC-MS)-based high throughput medium has been used to optimize one of the mutants in 20 different media combinations to increase the CsA yield (Abrol et al., 2022).
The carbon source provides energy for cell growth and metabolism and is a key factor affecting fungal growth and the synthesis of secondary metabolites (Li et al., 2015).It has been reported that Fusarium proliferatum (Matsush.)Nirenberg cultured with sucrose as the only carbon source increases the production of fumonisin but fumonisin production is inhibited when using fructose as the carbon source (Jian et al., 2019).Replacing glucose with fructose in the initial fermentation medium is more conducive to the production of pneumocandin B 0 , the precursor of the antifungal drug caspofungin, and biomass accumulation (Zhang et al., 2020).Reduced growth, thinner hyphae, and visible injury were observed early during the cultivation of Penicillium janczewskii K.W. Zaleski in a fructosecontaining medium, and the culture reached the maximum between days 12 and 15 (Pessoni et al., 2015).Some studies have attempted to replace CsA-producing strains with selected Aspergillus terreus Thom (FCBP58), and the CsA yield was effectively increased by optimizing the ratio of the carbon source to the nitrogen source in the medium (Tanseer and Anjum, 2011).However, few studies have analyzed how carbon sources affect the production of secondary metabolite CsA and its producing fungal mycelium growth.
Therefore, this aspect still needs further research.Some advancements in new technologies, like the various omics analyses, have become key tools to help us for these explorations and understand the molecular mechanisms that respond to changes in environmental conditions (Bi et al., 2018;Zhang et al., 2020).There are many successful studies here.The transcriptomic and proteomic analyses of the mycelium and fruiting bodies of Cordyceps militaris (L.) Fr. revealed differences in gene expression, including the cordycepin metabolism-related genes (Yin et al., 2012).The mechanism of the metabolic regulation and development of Pleurotus ostreatus sensu Stevenson fruiting bodies inhibited by a high CO 2 concentration was revealed using proteomics analysis (Lin et al., 2022).The global protein expression profile of white Hypsizygus marmoreus (Peck) H.E. Bigelow mycelium under heat stress has been studied using a label-free comparative proteomics technique, and the molecular mechanism of mushroom heat stress response was explained (Xu et al., 2021).Comparative proteomic analysis techniques combined with protein-to-protein interaction networks have been used to study the differences in the intracellular transcription mechanisms of carbon-de-inhibited and wild-type Pichia pastoris (Guillierm.)Phaff strains fed with three carbon sources.The cluster modules of the differentially expressed proteins (DEPs) associated with the carbon sources were obtained, and the co-expression relationships between the DEPs have been constructed (Shi et al., 2020;Wang et al., 2022).
In this study, fructose and sucrose were used as carbon sources during fermentation of T. inflatum.We determined the CsA yield between the two carbon sources and analyzed the differences in protein expression and metabolic pathways by proteomics analysis.The proteins regulating CsA synthesis related to carbon metabolism were explored.This study provides additional candidate genes or proteins for modification through metabolic engineering strategies and affords new ideas for obtaining more CsA high-yielding strains.
Tolypocladium inflatum NRRL 8044 was grown on potato dextrose agar (BD Difco, Sparks, MD, United States) for 2 weeks.The impurities and mycelia were removed by filtrating through sterile degreasing cotton with a thickness of about 0.5 cm to obtain the spore suspension.The spore suspension was diluted to a final concentration of 10 8 spores/mL.A 100 μL aliquot of the diluted spore suspension was removed and inoculated into 50 mL of liquid medium/250 mL conical flask at 25°C and 150 rpm in a constant temperature oscillating incubator for 3 days.The seed suspension was obtained after the incubation.Eight mL of the seed suspension was removed and inoculated into 250 mL of liquid medium/500 mL conical flask at 25°C at 150 rpm shaking for 9 days.Three biological replicates were used for each group.
The mycelia could be weighted and collected beginning on day 4.At the same time every day, 15 mL of culture broth including the fermentation product was removed and the mycelia were collected and placed in liquid nitrogen or stored at −80°C to determine hydrogen peroxide content, CsA production, total protein, and RNA.

Determination of CsA content
The Agilent 1,100 high-performance liquid chromatograph (Agilent Technologies Inc., Shanghai, China) was used to detect CsA, with a diode array UV detector.The separation was performed using the yuexu xtimate C18 column (particle size: 5 μm; column length: 4.6 × 250 mm); the sample size was 10 μL, and the detection wavelength was 210 nm.The parameter settings were column temperature of 40°C, mobile phase: A: water, B: acetonitrile (CAN), at a flow rate of 1 mL/min.The elution  (Yang et al., 2018).
The standard curve to determine the absolute weight of CsA was y = 2972.1 x -286.44,R2 = 0.9985, where x is the absolute weight of CsA, unit: μg; y was the integrated peak area, and the units are mAU•s.The CsA standard was 98% pure (Aladdin).

Determination of accumulated biomass
A 5 mL aliquot of the fermentation products was thoroughly dehydrated at low temperature in a freeze-drying machine (Scientz-18 N/A, Ningbo Scientz Biotechnology Co., Ltd., Ningbo, China).The products were weighed to dryness to determine dry weight (DW).

Total protein acquisition and mass spectrometry detection
The proteome was detected using label-free LC-MS/MS Quantitative Proteomics Analysis technology (Benagen Co., Ltd., Wuhan, China).All protein extraction and digestion methods and the MS detection for total proteins were described in a previous study (Yang et al., 2021).

Protein identification and DEP screening
The total protein sequences from the raw files were screened out with a false discovery rate (FDR) < 0.05 for mapping to the T. inflatum genomic database (Bushley et al., 2013).Raw data were normalized by Proteome Discovery software suite version 2.0 (Thermo Fisher Scientific, San Jose, CA, United States).Firstly, missing values were supplemented by the k proximity method.Then, median standardization was executed on intensity data.After that, EdgeR was used to screen DEPs with |log 2 (fold change)| > 1 and value of p<0.05.The protein expression difference of different samples was shown by a volcano plot constructed by TB tools.

Functional annotation of the DEPs
The DEPs were annotated and functionally enriched using Uniprot and Pfam.We also performed GO and KEGG analyses to annotate the DEPs.TBtools was used for the enrichment analyses.
The GO tool (http://geneontology.org; accessed on May 2023) was used and different terms were enriched.All terms were divided into three classes cellular components (CC), molecular functions (MF), and biological processes (BP).

Co-expression analysis of the DEPs
The String (search tool for the retrieval of interacting genes/ proteins) database was used to accurately identify and annotate all co-expressed DEPs. 1 The network of related DEPs in each sample was constructed using Cytoscape.The minimum required interaction score of confidence was 0.4.Proteins with high connectivity in the network were identified as hub DEPs and were believed to play core roles in co-regulating life processes.The DEPs that closely contacted the hubs were hub-surrounded DEPs, which assisted in determining the functionality of the hubs.

The proteomic data were validated by quantitative real-time PCR analysis
Total RNA and cDNA were prepared according to the description of the previous job (Yu et al., 2018).The threshold cycle values were normalized by the expression level of the 18S rRNA gene (Liu et al., 2018).RT-qPCR reactions were performed in a volume of 15 μL on a LightCycler ® 96 Real-Time PCR System (F.Hoffmann-La Roche Ltd., Basel, Switzerland) with ChamQ Universal SYBR qPCR Master Mix (Sparkjade, Shandong, China) according to the manufacturer's instruction.Expression levels were calculated according to the 2 -Δ Δ CT method.

Results and discussion
3.1 Changes in CsA yield and mycelium in fructose medium and sucrose medium, respectively CsA and mycelium production increased over time, and their production rates were different every day.The maximum CsA production rate in fructose medium was 73.38 μg CyA/5 mL fermentation broth/day (μg/5 mL/day) on day 6, and the rate of increase was the fastest.Compared with the rate on day 5 (17.60 μg/5 mL/day), the rate of increase was 316.93% [=(73.38-17.60)/17.60](Figures 1A,B).However, mycelium production increased slowly in the fructose medium on day 6 at a production rate of 2.72 mg DW mycelium/5 mL fermentation broth (mg DW/5 mL) (Figures 1C,D).
However, the CsA yield and production rate in sucrose medium were much lower than those in fructose medium.On day 6, the CsA production rate was 18.71 μg/5 mL/day, which was 1/3.92 of that in fructose medium (Figures 1A,B).However, the mycelium production rate reached a maximum of 10.58 mg DW/5 mL on day 6, which was 3.89 times higher than the mycelium production rate in fructose medium (Figures 1C,D).CsA production, CsA production rate, mycelium production, and mycelium production rate were recorded (Supplementary Table S1).
It was known that the production of CsA and the accumulation of mycelium had different priorities in these two different media Frontiers in Microbiology 04 frontiersin.orgaccording to the above results.On day 6, there was a higher priority for the production of CsA in the fructose medium, at this point fructose became a better carbon source for promoting CsA production, and the relatively high expression protein in the fructose fermentation product was more likely to be positive regulatory for the production of cyclosporine.However, mycelium preferentially accumulated in the sucrose medium on day 6, so sucrose was a better carbon source suitable for mycelium growth and the highly expressed protein in the sucrose fermentation was more likely to be a positive regulatory protein promoting mycelial growth (Figure 2).

Label-free LC-MS/MS technology was used to enable the high-throughput proteomic analysis of the samples separately cultured in fructose medium and sucrose medium
To screen the positively regulating proteins related to carbon metabolism of CsA synthesis and mycelial growth, fresh mycelium obtained on day 6 was selected as the test sample according to the results and named "Fru" and "Suc." The extracted total proteins were detected by label-free LC-MS/MS technology.A total of 3,227 proteins were identified and were median standardized (Supplementary Table S2).Principal component analysis indicated large differences between the groups and high similarities within the groups, indicating that the data were valid and could be used for further analysis (Figure 3A).

Screening of the DEPs
The DEPs were screened to compare the expression of Fru with Suc.A total of 244 DEPs were obtained and annotated (Supplementary Table S3-Total), including 74 highly expressed DEPs in the Fru (Supplementary Table S3-Fru) and 170 highly expressed DEPs in the Suc (Supplementary Table S3-Suc).The volcano plot shows the different effects of protein expression between the two groups and the distribution of the DEPs in each group.The different DEPs are circled and marked in yellow in the DEP list (Supplementary Table S3-Fru, Suc), and they may play a role in the trait formation of each group (Figure 3B).Four of the highly expressed DEPs, which are marked in green in the DEPs list (Supplementary Table S3-Fru), have been verified to be enzymes from the CsA biosynthetic gene cluster (Yang et al., 2018), including non-ribosomal peptide synthetase (NRPS, SimA, TINF00159), polyketide synthase (PKS, SimG, TINF00267), aminotransferase (SimJ, TINF00351), and ABC transporter (SimD, TINF00536).None of the CsA biosynthetic gene cluster proteins were highly expressed in Suc.These results further demonstrate that our analysis was accurate.

Functional analysis for the highly expressed DEPs in the Fru
Eighteen terms (p < 0.05) were significantly enriched in the GO analysis, including the BP terms of small molecules, such as carboxylic acid, oxoacid, organic acid, metabolic processes, lipid metabolic processes (p < 0.01), cellular/organic substance catabolic processes, organonitrogen compound catabolic processes, (organic substance) and metabolic processes, including the CC terms of cell periphery, endoplasmic reticulum, and obsolete cytoplasmic part and the MF term of catalytic activity (p < 0.01).The DEPs annotations, expression ratios, and p-values are listed (Figure 4A; Supplementary Table S4-1).Five pathways (p < 0.05) were enriched in the KEGG pathway enrichment analysis.Three pathways were involved in metabolism, including amino acid metabolism (p < 0.01), lipid metabolism, and carbohydrate metabolism.The exosome pathway belonged to the Brite hierarchy.The last pathway was transport and catabolism, which belonged to the CP.The detailed KEGG information is listed (Figure 5A; Supplementary Table S5-1).
Nineteen highly expressed DEPs were functionally related to Fru and connected in the network with the help of a co-expression analysis (Figure 6A).Twelve of these DEPs were related to small molecules, such as monocarboxylic acid, lipid, amino acid, and carbohydrate, metabolic processes (GO: 0044281, 0032787, 0006629, 0044255, 0019752, 0043436, 0006082, 0071704; KEGG: B 09103, B 09105, B 09101).The hub DEPs were aldehyde dehydrogenase (TINF03264), long-chain-fatty-acid-CoA ligase 1 (TINF02972), 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing] (TINF06393), and malate synthase (TINF00525).Among them, polyketide synthase (TINF00267) was a major enzyme in the CsA gene cluster.The TINF02972 hub positively regulates CsA synthesis because it has a direct co-expression relationship with TINF00267.Ten of the DEPs were related to organic substances, particularly organonitrogen compounds, catabolic processes (GO: 1901575, 0009056, and 1,901,565), and transport and catabolism (KEGG: B 09141).The hub DEPs were TINF06393, TINF00525, TINF03264, and catalase (TINF06528).Notably, almost all DEPs involved in this network belonged to the obsolete cytoplasmic part (GO:0044444) or exosome (KEGG: 04147), indicating that these proteins were less relevant to primary metabolism, and more relevant to secondary metabolism, further reminding us that these proteins are related to the synthesis, metabolism, and transportation of secondary CsA metabolites.The DEPs involved in the network are listed and the hub DEPs are marked in red (Table 1).
Many studies have improved the CsA yield.These mostly rely on the traditional method of mutagenesis and screening of high-yielding strains, but results using this method are random and counterproductive (Domratcheva et al., 2018).Although filamentous fungi could undergo some natural mutations as they grow, such as low-frequency gene recombination could be produced by parasexuality, some recombinants could be obtained for screening strains with a higher yield of secondary metabolites to a certain extent, but this evolutionary speed is not as fast as bacteria, which is far from meeting the needs of production (Meyer, 2008).CsA is a secondary cyclopeptide metabolite (Zhang et al., 2017) composed of 11 amino acids (Molnár et al., 2010).CsA synthesis requires the unusual amino acid (4R)-4-[(E) -2-butenyl]-4-methyl-L-threonine (Bmt) as a substrate (Kirihata et al., 1995), and MeBmt (partially methylated Bmt) was marked out (Figure 7).However, CsA chemical synthetic methods are mostly limited by the difficulty of obtaining Bmt, because Bmt does not exist in natural medium, and its yield is very low in Bmt-producing fungi.In addition, Bmt is difficult to isolate and purify from complex products.Some researchers have been working to obtain MeBmt through a chemical synthesis approach (Rolt et al., 2019).However, this method involves many steps, many intermediate products, and a low final yield, and is difficult to put into industrial production.
The full biosynthetic mechanism of CsA has been analyzed at the genetic level.The CsA biosynthetic gene cluster has been suggested to include 12 genes encoding enzymes, including NRPS (SimA, The overall research idea to illustrate the correlation between CsA production, mycelium growth, and protein expression.TINF00159) responsible for assembling the 11 amino acid substrates of CsA and PKS (SimG, TINF00267) to mediate Bmt production (Yang et al., 2018).Many attempts have been made to obtain highyielding engineered strains by expressing important secondary metabolites genes or gene clusters in a heterologous host that are produced at low levels by the original strain (Lan et al., 2019).However, NRPS and PKS are usually abundant in filamentous fungi, so cloning and processing large DNA fragments or complete gene clusters to achieve their hetero-expression is a challenge (Nielsen et al., 2019).Mining more genes or proteins that positively regulate CsA synthesis from several perspectives will indicate the direction to obtain engineered strains with higher CsA yields and provide more space for genetic manipulation.
In addition to these proteins analyzed, other proteins may play vital roles in the regulation of CsA synthesis.It has been confirmed that cytochrome P450 (SimI, TINF00470) and aminotransferase (SimJ, TINF00351) are involved in Bmt synthesis (Yang et al., 2018).Two DEPs highly expressed in Fru were functionally similar.Copper radical oxidase (TINF06995) is a biocatalyst for the selective oxidation of primary alcohols to aldehydes and has a wide range of specificities for aliphatic compounds, which have a similar function to TINF00470 (Cleveland et al., 2021).The other DEP was an aminotransferase (putative, TINF08869), which was the same as TINF00351.
Overexpressing the basic leucine zipper (bZIP) transcription regulator (SimL, TINF00394), which directly regulates the expression of the CsA gene cluster, improves CsA production (Yang et al., 2018).Interestingly, three highly expressed DEPs regulated DNA transcription in Fru.The Myb transcription factor (TINF04891), which can be activated by bZip transcription factors, participated in transducing the aerial stimulus signal.Both factors jointly activate a C(2)H(2) zinc finger transcription factor, which plays a central role in fungal growth and virulence (Garzia et al., 2010;Lee et al., 2021).TINF04891 may participate in the regulation of CsA biosynthesis.Another protein containing the critical features of the tristetraprolin zinc finger domain has been linked to the control of pheromone signal transduction and the coordination of mitosis (Cuthbertson et al., 2008).The CCCH zinc finger DNA binding protein (TINF00841) may regulate the CsA biosynthetic process.The transcriptional activator ALCR, which is a transcriptional activator in the ethanol utilization pathway of filamentous fungi, is a DNA-binding protein with a helixturn-helix structure (Kulmburg et al., 1992).The helix-turn-helix domain-containing protein (TINF02756) may also be a transcriptional activator of CsA biosynthesis.The ABC transporter (SimD, TINF00536) in the CsA gene cluster may facilitate the production of more CsA and increase tolerance to the fungus by transferring CsA to reduce its concentration (Yang et al., 2018).The WSC domain-containing protein (TINF08949) may have a similar function as SimD because it localizes in the vacuoles and cell wall/membrane of the filamentous fungus Beauveria bassiana and, hence, has been linked to cell membrane-and vacuole-related cellular events (Tong et al., 2019).The short-chain dehydrogenase/reductase SDR (TINF06213, TINF03230) could also enhance fungal tolerance to secondary metabolites by the non-toxic transformation of secondary metabolites (Xing et al., 2021).It may also participate in CsA transformation.

Highly expressed DEPs in Suc
Industrial fermentation to produce secondary metabolites often requires a segmented culture of engineered fungi.More secondary metabolites are produced when the mycelium reaches a particular (A) GO analysis of the highly expressed DEPs in the Fru.(B) GO analysis of the highly expressed DEPs in the Suc.The GO terms were distributed into three classes, biological process (•), molecular function (▴), and cellular component (◼).The larger the graph, the more DEPs were involved.p-values ranged from 0 to 0.05, indicating warmer colors and more significant differences.The enrichment score (ES) is the maximum statistical value accumulated by the degree of association between each protein in the protein set and the phenotype, representing the degree of association between the whole protein set and the phenotype.abundance.A previous study was aimed at optimizing the culture medium for biomass production and phenolic compounds using Ganoderma lucidum.The culture was optimized in two stages.After the maximum biomass production was reached under optimal conditions, more phenolic compounds were obtained (Zárate-Chaves et al., 2013).We analyzed the highly expressed DEPs in Suc medium, which are mostly involved in the regulation of T. inflatum mycelial growth.
Seventy-four terms (p < 0.05) were significantly enriched in the GO analysis, including the BP terms of obsolete oxidation-reduction processes and obsolete oxidoreduction coenzyme metabolic processes, small molecules, such as antibiotic and pyridinecontaining compounds, biosynthetic or metabolic processes (p < 0.01), cellular response to xenobiotic/chemical stimulus/stress, response to inorganic substance/oxidative stress and detoxification, xenobiotic catabolic/metabolic processes, obsolete cofactor metabolic  The hub proteins were marked in yellow.
The cell walls of filamentous fungi contain chitin, protein, mannan, and amorphous glucan.Six highly expressed DEPs related to fungal cell walls occurred.Four of these DEPs were involved in the formation of cell wall components and maintaining cell wall integrity.The beta-flanking protein (TINF04729) was a conserved genomic neighbor localized within a recently identified metabolic cell wall gene cluster in genomes of Aspergillus spp., which may participate in cell wall biosynthesis (Guerriero et al., 2016).The carbohydratebinding WSC (TINF05352) was not recognized in substrates but attaches the enzyme to plant and/or fungal cell walls, and may  be potentially involved in β-glucan remodeling to maintain cell wall integrity and participate in the stress response (Wawra et al., 2019).The oxidoreductase, 2OG-Fe(II) oxygenase family (putative, TINF05005) may affect the composition of the secondary cell wall (Fang et al., 2012).The sphingolipid long-chain base-responsive protein PIL1 (TINF07009) inhibits protein kinases involved in signaling pathways for cell wall integrity (Delom et al., 2006).The other two DEPs were components of the cell wall and related to different cell wall functions.The chitin-binding, domain 3 (TINF05053) has been predicted to be localized in the cell wall (Zhan and Guo, 2015).Cyanovirin-N (TINF06073) in filamentous ascomycetes is a nonsecretory monodomain protein and a multidomain protein bearing functionally related modules, such as peptidoglycans and chitin-binding domain LysM, in the cell wall (Percudani et al., 2005).
Lipid molecules, such as cholesterol and ergosterol, are found in eukaryotic cell membranes.Eight DEPs were related to membrane and transport.Four of these DEPs were involved in the formation of the plasma membrane.Squalene monooxygenase (TINF07977) is an important enzyme involved in the synthesis of ergosterol, cholesterol, and phytosterols (Zare et al., 2014).The BAR domain protein (TINF01359) is a membrane-shaping protein, which determines organelle biogenesis, membrane trafficking, cell division, and cell migration (Frost et al., 2009).The ankyrin repeats domain-containing protein (TINF00399) is localized on the cytoplasmic membrane during transient expression in onion epidermal cells (Zhang et al., 2010).The oxysterol binding protein (TINF02166) is an important non-vesicular trafficking protein involved in the transportation of lipids in eukaryotic cells, and may also participate in membrane formation (Qiu and Zeng, 2019).Another four DEPs were involved in the formation of membrane organelles, such as vacuoles and vesicles, as well as transportation processes.Carboxypeptidase (TINF00103) has been used as a marker enzyme for investigations on the intracellular transport of vacuolar proteins and vacuolar biogenesis in S. cerevisiae (Ohsumi et al., 2001).The hsc70 cochaperone (SGT) (putative, TINF09583) bends membranes based on their ability to oligomerize.This activity promotes endosomal microautophagy and the turnover of specific synaptic proteins (Uytterhoeven et al., 2015).The apolipoprotein/apolipoprotein (TINF08525) is involved in phagocytosis, and possibly pattern recognition (Whitten et al., 2004).The vesicle-fusing ATPase (TINF02607) serves a dual role in vacuolar integrity to regulate vacuole fusion and fission reactions in yeast (Qiu, 2012).Five DEPs were related to mycelial growth and development.The cipC-like antibiotic response protein (putative, TINF04883) was exclusively found in the hyphal morphotype, which enables invasive growth of Aspergillus fumigatus during infection (Bauer et al., 2010).The protein kinase activator (Mob2) (putative, TINF07215) is crucial for normal hyphal development (Gutiérrez-Escribano et al., 2011).Two DEPs targeting the endoplasmic reticulum (ER), the short-chain dehydrogenase/reductase family protein (TINF05550), and signal recognition particle subunit SRP72 (TINF01479) are related to cell death, defense responses, and protein transport (Brown et al., 1994;Zheng et al., 2022).The glia maturation factor beta (TINF07923) is a regulator of the actin cytoskeleton with a unique role in remodeling the actin network architecture, and it has roles in controlling actin filament spatial organization and the dynamics underlying cell motility, endocytosis, and other biological processes (Goode et al., 2018).

Quantitative real-time polymerase chain reaction (PCR) validation of DEP expression
Gene expression is largely consistent with protein expression.We used RT-qPCR to determine the gene expression levels (mRNA content) of seven key DEPs, including the hub DEPs in the pathways of carbohydrate and monosaccharide metabolism, protein-containing complex organization and mRNA binding, amino acid metabolism, (ribose) and phosphorus and purine ribonucleotide metabolism to verify the accuracy of our proteomics results.The primers for RT-qPCR analysis are listed in Supplementary Table S6-1.The results showed that the changes in gene expression (Supplementary Table S6-2) were consistent with changes in protein expression (Supplementary Table S6-3), indicating the reliability of our proteomic results and the accuracy of the DEP screening (Figure 8).

Conclusion
CsA production and the accumulation of mycelia differed in Fru and Suc media.The difference was most obvious on day 6 of culture when CsA was produced in the Fru medium and mycelium preferentially grew in the Suc medium.The mycelium proteome in these two groups was determined and compared.The highly expressed mycelial proteins cultured in Fru medium were involved in the regulation of CsA production, metabolism, and transportation.These DEPs participated in the processes of small molecules, such as carboxylic acid, oxic acid, and organic acid metabolism; lipid metabolism; catabolic processes of organic substances, particularly organo-nitrogen compounds; CsA transport and exocrine-derived functions of the ER, exosomes, and obsolete cytoplasmic parts; regulating the synthesis of the CsA substrate Bmt; functions of transcriptional regulators.The highly expressed DEPs in the Suc medium were involved in the regulation of mycelial growth.They were mainly involved in the processes of the abandoned REDOX or coenzyme metabolism; small molecules, such as antibiotics, pyridine-containing compounds, amino acids, carbohydrates, biosynthesis or metabolism; response to stress, such as exogenous stimuli/inorganic/chemical stimuli, exogenous catabolism, oxidative stress, and detoxification; genetic information or epigenetic changes, such as changes in nuclear

FIGURE 1
FIGURE 1 Comparison of CsA production and mycelial production from different carbon sources.(A) The change in the CsA yield from days 4 to 9 was cultured separately in fructose and sucrose media.(B) The CsA production rate from days 5 to 9. (C) The change in mycelial production corresponded to the change in CsA yield.(D) The mycelial production rate corresponded to the CsA production rate.★: There were different priorities in CsA production and mycelium production on the 6th day.

FIGURE 3 PCA
FIGURE 3 PCA analysis and volcano plot.(A) PCA plot shows the clustering of the samples based on their similarities.(B) The volcano plot shows the differential expression of the proteins in Fru and Suc.Each dot represents one protein.The horizontal coordinate measures the difference, and the vertical coordinates measure the significance of the difference.The highly expressed DEPs are blue in the Fru and red in the Suc.DEPs with relatively large and significant differences are circled.

FIGURE 4
FIGURE 4 FIGURE 5 (A) KEGG pathway analysis of the highly expressed DEPs in the Fru.(B) KEGG pathway analysis of the highly expressed DEPs in the Suc.The color of the graph represents the value of p ranging from 0 to 0.05.The size of the graph represents the number of the included DEPs.

FIGURE 6
FIGURE 6 The co-expression network diagrams of the functionally related DEPs in the Fru (A) and the Suc (B).Circles represent one DEP and size indicates the connectivity value of the proteins.The hub DEPs with high connectivity values are marked in deeper colors.The straight lines represent edges and the width of a straight line indicates the weighted value.The larger the weighted value between two DEPs, the closer their relationship.

FIGURE 8
FIGURE 8 Quantitative real-time PCR validation of the proteomic results.(A) The mRNA expression of the seven hub DEPs.(B) The protein expression of the seven hub DEPs.***: p-value <0.001, **: p-value <0.01, *: p-value <0.05.The protein expression changes are consistent with those of their mRNAs.

TABLE 1
The highly expressed DEPs of Fru in a network.

TABLE 2
The highly expressed DEPs of Suc in a network.