Healing wounds, defeating biofilms: Lactiplantibacillus plantarum in tackling MRSA infections

Introduction Methicillin-resistant Staphylococcus aureus (MRSA) infections are well-known hospital-borne infections and are a major contributing factor to global health concerns of antimicrobial resistance due to the formation of biofilms. Probiotics are known to assist in the healing of wounds through immunomodulation and also possess anti-pathogen properties via competitive inhibition. The probiotic bacterium, Lactiplantibacillus plantarum MTCC 2621 and its cell-free supernatant (Lp2621) have previously been reported to have antibacterial, excellent antioxidant, and wound healing activity in in vitro conditions and wounds contaminated with S. aureus in mice. Methods In the current study, we evaluated its anti-MRSA, biofilm inhibition and eradication efficacy, immunomodulatory activity in THP-1 cells, and wound healing potential in wounds contaminated with MRSA infection in mice. Results In agar well diffusion assay, Lp2621 showed anti-MRSA activity and revealed dose-dependent inhibition and eradication of biofilm by crystal violet assay as well as by Confocal Scanning Laser Microscopy (CLSM) analysis. Further, Lp2621 showed immunomodulatory activity at varied concentrations as measured by IL-6 and IL-10 gene expression in THP-1 cells. Similar findings were observed in serum samples of mice after treatment of excision wound contaminated with MRSA infection by Lp2621 gel, as evident by expression of IL-6 (pro-inflammatory) and IL-10 (anti-inflammatory) cytokines. Conclusions Overall, our results show that Lp2621 has potent anti-MRSA and antioxidant properties and can prevent and eliminate biofilm formation. It also showed promise when applied to mice with MRSA-infected wounds.

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) infections are well-known hospital-borne infections and are a major contributing factor to global health concerns of antimicrobial resistance due to the formation of biofilms.Probiotics are known to assist in the healing of wounds through immunomodulation and also possess anti-pathogen properties via competitive inhibition.The probiotic bacterium, Lactiplantibacillus plantarum MTCC and its cell-free supernatant ( Lp) have previously been reported to have antibacterial, excellent antioxidant, and wound healing activity in in vitro conditions and wounds contaminated with S. aureus in mice.
Methods: In the current study, we evaluated its anti-MRSA, biofilm inhibition and eradication e cacy, immunomodulatory activity in THP-cells, and wound healing potential in wounds contaminated with MRSA infection in mice.

Results: In agar well di usion assay, Lp
showed anti-MRSA activity and revealed dose-dependent inhibition and eradication of biofilm by crystal violet assay as well as by Confocal Scanning Laser Microscopy (CLSM) analysis.Further, Lp showed immunomodulatory activity at varied concentrations as measured by IL-and IL-gene expression in THP-cells.Similar findings were observed in serum samples of mice after treatment of excision wound contaminated with MRSA infection by Lp gel, as evident by expression of IL-(pro-inflammatory) and IL-(anti-inflammatory) cytokines.

Introduction
Wound healing is a multi-faceted biological phenomenon that allows the body to repair damaged tissue.Cells (keratinocytes, fibroblasts, and macrophages) are directed to the site of the wound as a result of an inflammatory response and initiate the healing process through interaction with stem cells.This leads to a variety of biochemical processes that allow the body to repair physical tissue (Eming et al., 2014).Wounds are most commonly infected with Staphylococcus aureus (Tong et al., 2015), which can develop resistance to methicillin and antibiotics (lactam) through the manifestation of a foreign penicillin-binding protein (PBP, PBP2a) (Stapleton and Taylor, 2002).MRSA is a grave public health problem worldwide, and infections caused by these germs are among the most difficult therapeutic challenges.Infections caused by bacterial biofilms are becoming more widespread around the world.Bacteria in biofilms are frequently resistant to standard antibiotic therapy because, over time, they have developed a number of defensive mechanisms against conventional antibiotic therapy (Xiu et al., 2021).All MRSA strains are capable of microplate attachment and biofilm formation.Developing biofilms and antibiotic resistance are crucial to the success of S. aureus pathogens in both hospitals and community surroundings (Akbari-Ayezloy et al., 2017).Methicillin-resistant Staphylococcus aureus (HA-MRSA & CA-MRSA) are hospital-and community-associated infections and are additional categories for MRSA infections that can affect different parts of the body and vary in severity from minor skin contaminations to more lethal infections such as bacteremia, skin and soft tissue infections, bone and joint infections, endocarditis, pneumonia, and sepsis (Siddiqui and Koirala, 2022).Approximately 20-50 cases/100,000 population for S. aureus bacteremia are reported annually, which alone causes more causality than AIDS, TB, and viral hepatitis combined (Hal et al., 2012).
MRSA has emerged in recent years primarily as an isolated bacterium in wound cultures (Naylor et al., 2001;Scriven et al., 2003;Reddy et al., 2007) that can dramatically slow wound healing and increase the risk of complications (Thimmappa et al., 2021).First, the inflammation caused by the bacteria can damage healthy tissue and impede the healing process.Second, MRSA can form a biofilm on the site that protects the germs from the body's immune response and makes it difficult for antibiotics to reach and destroy the germs (Simonetti et al., 2022).Treatment of MRSA-infected wounds usually involves a combination of antibiotics and wound care.Vancomycin and other MRSA-fighting antibiotics may be prescribed to treat the infection (Cong et al., 2020).
To effectively treat wounds and infections, a variety of biological strategies have been developed and adopted.These include the use of various scaffolds, carriers, and patches derived from algae, as well as stimuli-responsive hydrogels and ceria (CeO 2 )-modified nanoparticles (Zhong et al., 2021;Li et al., 2022;Ma et al., 2022).Only a small number of antibiofilm strategies are being used in clinical settings, and the rest still need to be developed significantly.The use of nanotechnology-based treatments to fight bacterial biofilm infections has been suggested by Xiu et al. (2021).
Probiotics improve health through oral or topical administration and may assist in the reduction of the risk of MRSA infection and improve outcomes for MRSA-infected patients (Cella et al., 2023).S. aureus and clinical MRSA isolates are prevented from growing in vitro by a wide range of lactobacilli and bifidobacteria strains (Sikorska and Smoragiewicz, 2013).However, to fully assess the potential benefits of probiotics in preventing or treating MRSA infections, more research is warranted.Probiotics are most likely to be ineffective in neonates and/or in individuals with certain clinical problems such as cancer, diabetes mellitus, leaky gut syndrome, and convalescence after organ transplantation (Kothari et al., 2019).Probiotic strains may have antibacterial .
/fmicb. .activity against MRSA.One study found that a probiotic strain called Lactobacillus reuteri suppressed the growth of MRSA in vitro (Prince et al., 2012), while Lactobacillus plantarum prevented the spread of MRSA in mice (Sikorska and Smoragiewicz, 2013).The bioactive molecules present in the cell-free supernatant of lactic acid bacteria exhibited anti-staphylococcal effects by upregulating β-defensin and modulating cytokines and chemokines during wound healing phases (Ong et al., 2020).Hydrogels loaded with Lactobacillus rhamnosus (HPF@L.rha)significantly decreased infection and inflammation, encouraged the production of new collagen and epithelium, and accelerated wound healing (Mei et al., 2022).Mice given curcumin-loaded solid lipid nanoparticles (CSLNs) along with a probiotic dressing (L.plantarum UBLP-40) demonstrated faster wound closure and less bioburden in the wound area.Through immunomodulation, antibacterial activity, suppression of pathogenic toxins, and anti-inflammatory attributes, these therapies also aided in the promotion of wound healing (Sandhu et al., 2023).
Previously, the cell-free supernatant of Lactiplantibacillus plantarum MTCC 2621 from our lab demonstrated the antimicrobial activity, antioxidant properties, and woundhealing potential of Lp2621 (Dubey et al., 2021).In this study, we sought to evaluate anti-MRSA, radical scavenging, biofilm inhibition, and eradication activities of Lp2621.We also provide evidence showing that Lp2621 has the potential to heal excisional wounds infected with MRSA 831 (Graphical abstract).
. Culture of L. plantarum MTCC and preparation of Lp L. plantarum MTCC 2621 was obtained from the Microbial Type Culture Collection (MTCC) (CSIR-IMTECH).The MRSA 831, a clinical strain, was a gift from Dr. Hemraj Nandanwar's laboratory at CSIR-IMTECH.The MRS broth was used to culture the L. plantarum at 37 • C, and the grown culture was centrifuged (5,000 rpm, 10 min) for collection of cell free supernatant (CFS) and stored at 4 • C and filtered through a 0.22 µm filter for animal tissue culture experimentation.The MRSA strain was cultured in Mueller Hinton Broth (MHB).The Institutional Biosafety Committee approved the use of MRSA 831 in experiments.
The experimental design of the study is illustrated in a flowchart in Figure 1.

. Anti-MRSA activity of Lp
The antibacterial activity against MRSA 831 was evaluated using the agar well diffusion technique.The MRSA strain was inoculated on the nutrient agar (10 6 CFU/ml).Six mm diameter wells were punched with a sterile well borer, and 80 µL of Lp2621 was added into the test well.Vancomycin (positive control) and MRS broth (negative control) were used and incubated at 37 • C. The inhibitory zone diameter was calculated with a ruler after 24 h (Dahiya and Purkayastha, 2012).

. Biofilm inhibition and eradication activity of Lp
The method of Yang et al. (2021) was used to test whether LP2621 could inhibit the formation and eradication of MRSAinduced biofilms (Yang et al., 2021).Briefly, MRSA 831 overnight culture was suspended in MHB, 5 × 10 5 CFU/ml were inoculated in 96-well plates, and Lp2621 was incubated for 24 h at 37 • C. The media used was taken out, and the biofilms were rinsed three times with 1X PBS and fixed with 99% v/v methanol for 15 min at room temperature.Crystal violet 0.1% w/v was applied to stain the biofilm for 10 min at room temperature in the dark.

Biofilm inhibition (%) =
Control absorbance − Test absorbance Control absorbance × 100 (1) The method was followed with some changes used by Perumal and Mahmud (2013) for the biofilm eradication study of Lp2621.Briefly, MRSA831 overnight culture was inoculated at a 96-well plate and kept for biofilm production at 37 • C for 48 h.1X PBS (pH 7.4) was used to wash the wells.The established biofilm was treated with Lp2621 and incubated at 37 • C for 24 h.The media used were taken out, and the biofilms were rinsed three times with 1X PBS, and 99% v/v methanol was used for fixation for 15 min at 25 • C. Crystal violet 0.1% w/v was applied to fixed biofilm for 10 min at 25 • C and kept in the dark.

. Confocal scanning laser microscopy analysis
Confocal microscopy was used to visualize the MRSA-formed biofilm morphology.MRSA 831 was cultured overnight in MHB at a concentration of 5 × 10 5 CFU/ml (0.01 OD).The culture was then inoculated onto poly L-lysine coated coverslip into a 6-well plate and supplemented with different dilutions of Lp2621, i.e., 50, 12.5, and 0.78% (v/v) for 12-14 h.The medium was removed, and the biofilms were washed with 1X PBS (pH 7.4) three times and stained with 3 µM SYTO9 for 15-20 min at room temperature in the dark.The coverslips were washed after staining with 1X PBS (pH-7.4)three times and placed onto a slide (on 10 µL live antifade reagent added), and mounted onto sides.The prepared slides were then visualized under confocal scanning laser microscopy at 60X (oil) magnification. .Antioxidant properties DPPH assay is a reliable and accurate method to assess the ability of antioxidants to scavenge free radicals.We followed the DPPH method of free radical scavenging activity for evaluating the antioxidant potential of Lp2621 used by Sancineto et al. (2016) with slight modifications (Sancineto et al., 2016).In brief, the CFS was diluted two-fold in methanol up to 0.78% v/v and mixed with a methanol solution of DPPH (25 µg/ml).The reaction was allowed for 15 min, 30 min, and 24 h in the dark at room temperature.Ascorbic acid (AA) (60 µg/ml) was used as a positive standard, while the control had DPPH radical and methanol solution.The sample and methanol combination were used as sample blank.
The percentage inhibition of DPPH was calculated using the following equation: .Gene expression analysis of IL-and IL-. .THP-cell cultivation THP-1 (human leukemia monocyte cell line T-helper) cells were revived using RPMI 1640 medium (Gibco), with 10% FBS (Gibco), and antimicrobial 1% penicillin-streptomycin (Gibco) and maintained in CO 2 incubator at 37 • C and 5% CO 2 .Cells were plated in a 6-well culture plate (10 6 cells/ml) and incubated with PMA (4-phorbol 12-myristate 13-acetate) (20 ng/ml) for 24 h at 37 • C and 5% CO 2 in a CO 2 incubator for differentiation to macrophages.The differentiated THP-1 cells were treated with different concentrations of Lp2621, and RNA was extracted from the treated cells for analysis of gene expression of IL-6 and IL-10 by qRT-PCR.

. . Extraction of RNA and cDNA synthesis
TRIzol was used to extract total RNA from the cells.Concentration and purity of RNA were checked on a TECAN UV-VIS spectrophotometer (Nanodrop Technologies, Thermo Fisher Scientific), and the cDNA was made using reverse transcription with a high-capacity cDNA synthesis kit (Applied Biosystems cat no.4368814).The qRT-PCR was carried out on a qTOWER3G (Analytik Jena).The primers used for different genes have been given in Table 1.GAPDH, the housekeeping gene, was used as an .

Rheological properties of Lp gel
The bacterial culture of L. plantarum 2621 containing 1 × 10 9 CFU/ml was centrifuged for 10 min at 5,000 rpm, and the cellfree supernatant (CFS) was taken.A total of 2% carboxy methyl cellulose (CMC) was added to CFS and mixed continuously until a homogenous gel of Lp2621 was formed.The gel was kept at 4 • C for subsequent use.Rheological properties of the gel were conducted utilizing a rheometer (MCR 102, Anton Paar) equipped with a Peltier plate temperature-controlled device for precise thermoregulation.A parallel plate (40 mm) with a set gap of 1.0 mm was used to study the viscosity flow curve under the shear range between 0.001-1,000 s −1 to determine the flow behavior of the gel (Srivastava and Choudhury, 2021).

. Lp gel improved healing of wounds contaminated with MRSA infection in mice
The Institutional Animal Ethics Committee of CSIR-Institute of Microbial Technology (CSIR-IMTECH) approved the use of animals (IAEC/22/06).Experiments on mice were conducted as per the guiding principles of the Committee for the Control and Supervision of Experiments on Animals (CCSEA), Ministry of Fisheries, Animal Husbandry, and Dairying, India.
BALB/c mice (8 weeks) were obtained from the IMTECH Centre for Animal Resources and Experimentation (iCARE) of CSIR-IMTECH.Mice were housed in IVC cages in the ABSL-2 facility of iCARE, and pelleted food and water were provided ad libitum.Mice were randomly assigned to different groups before the experiment for a period of 1 week to acclimatize.In our previous study, we showed the beneficial effect of Lp2621 on normal wounds as well as wounds contaminated with S. aureus infection (Dubey et al., 2021).
Since contamination of wounds with MRSA is difficult to treat, we next pondered whether Lp2621 would be effective in the treatment of such wounds.We, therefore, designed an experiment wherein excision wounds were created in mice, and the wounds were contaminated with MRSA infection, followed by treatment with Lp2621 gel.In brief, mice were divided into the following five groups (n = 8 mice per group): negative (only wound), disease (wound + infection), vehicle (wound + infection + CMC), positive (wound + infection + betadine), and Lp2621 group (wound + infection + Lp2621).The mice were acclimatized for a week, anesthetized, dorsal hair removed, wiped, and disinfected (70% ethanol).The previous method for the creation of a wound was followed (Dubey et al., 2021).The wound was infected with 1 × 10 5 CFU/ml of MRSA 831 (Hoffmann et al., 2020) with slight modification, and each group of mice was topically treated with respective treatment for up to 21 days (twice a day) after 24 h of infection.On days 0, 7, 14, and 21, images of wounds were taken by DSLR camera and processed using ImageJ software for wound area calculation.

Healed area Total area
× 100 (4) The wound tissues and blood samples were collected from different groups of mice on days 7 and 14 for histopathological examination and cytokine analysis, respectively.The tissues were fixed in 10% neutral buffered formalin, cleaned in xylene, dehydrated with graded alcohol, molded in paraffin, and sections of 4-5 µm thickness were prepared and stained with hematoxylin and eosin (H&E) and were then examined under a light microscope.
The serum samples were used for immunomodulatory analysis.Cytokine analysis was performed using a BD multiplex CBA kit through FACS.

. Statistical analysis
The statistical analysis was done using a one-way analysis of variance (ANOVA).The results are expressed as the mean ± SD.

Results . Anti-MRSA activity of Lp
Lp2621 exhibited anti-MRSA activity in agar well diffusion assay.The measured zone of inhibition for Lp2621 was 11.66 ± 0.57 mm, and for vancomycin (positive control), it was 19.00 ± 1.00 mm (Figure 2).

. MRSA biofilm inhibition and eradication activity of Lp
The underlying mechanisms controlling the biofilm formation of MRSA strains in various types of human infections are not very clear.Lp2621 could inhibit biofilm formed by MRSA in a dose-dependent manner (Figure 3A).At higher concentrations (100-12.5% v/v), it showed significantly higher inhibition of 77%, whereas the inhibition was 33% at lower concentrations (0.78% v/v).Moreover, higher concentrations of Lp2621 (100, 50, and 25% v/v) were found to be highly effective in eradicating and removing the biofilm formed by MRSA 831 (>80%) (Figure 3B).To further investigate the antibiofilm effect of Lp2621 against MRSA 831 and observe the morphological changes in biofilm, CLSM was performed.CLSM images (Figure 3C) showed thick biofilms in MRSA 831 containing live cells; however, the thickness and viability of cells significantly decreased after treatment with different concentrations of Lp2621 (dose-dependent).The biofilm

Agar well di usion technique to measure Lp
′ s e ectiveness against MRSA .Data from three separate biological investigations were presented in triplicate. .

Antioxidant properties of Lp
Having observed the anti-MRSA effect of the cell-free supernatant, we next planned to explore the antioxidant potential of Lp2621 using a DPPH assay.Ascorbic acid was taken as a positive standard (60 µg/ml).Figure 4 shows the scavenging activity of Lp2621 for free radicals.DPPH inhibition mediated by Lp2621 was highly significant at higher concentrations (50, 25, and 12.5 v/v) at 15 and 30 min compared to the control (methyl alcohol and DPPH).At a concentration of 50% v/v, Lp2621 showed 76 and 81% inhibition, respectively, after 15 and 30 min of reaction time (p < 0.0001).Interestingly, after 24 h of reaction, Lp2621 exhibited better scavenging activity at various concentrations, and maximum inhibition was 90% at 25% v/v (p < 0.0001).The effective concentration (EC 50 ) was found to be 95.04 v/v (at 15 min), 40.94 v/v (at 30 min), and 5.94 v/v (at 24 h) at respective time points.

. Modulation of relative gene expression of IL-and IL-by Lp
THP-1 cells are the most commonly used cells for immune response (inflammation) studies.The uniform genetic basis of THP-1 minimizes the degree of variability in cell phenotype, which increases the reproducibility of results.qRT-PCR was used to verify gene expression of IL-6 and IL-10 after incubating differentiated THP-1 cells with various concentrations of Lp2621 for 24 h.Although there were changes in mRNA expression of IL-10 (increased at 25% v/v) and IL-6 (decreased at various concentrations 12.5-50% v/v) after treatment with Lp2621 as compared to the control gene, i.e., GAPDH, these changes in expression levels were statistically non-significant (Figure 5).

. Rheological profile of Lp gel
The flow behavior of the Lp2621 gel was determined by rheological tests.From the rheological data (Figure 6A), it was found that the viscosity decreased with increasing shear rate, indicating that it was a pseudoplastic, non-Newtonian material.
Here, the viscosities at zero (η0) and at infinite shear rate (η∞) were determined by fitting the Carreau-Yasuda model.The η∞ values for CMC were 123.84 mPa•s and for CFS-CMC 13.972 mPa•s, showing that the viscosity of the gel decreased, which is desirable for topical applications.
. Lp gel improved healing of wounds contaminated with MRSA infection in mice MRSA infections are difficult to treat as these bugs are resistant to most antibiotics.Therefore, we wanted to investigate whether treating wounds contaminated with MRSA with Lp2621 could shorten wound healing time.Topical application of Lp2621 gel resulted in a recovery of wound infected with MRSA.Faster wound healing was observed in mice treated with Lp2621 and betadine on day 7 as compared to CMC (vehicle) treated and the diseased control (p < 0.0001) untreated mice, and at day 14, wound healing was statistically more significant in Lp2621 and betadine treated mice than other groups.This is evident by an increase in the percent wound contraction in mice treated with our gel and betadine (Figures 6B, C).

. . Histopathological examination
The results of histopathologic evaluation of the wound tissues of mice from each treatment group on days 7 and 14 after wounding are shown in Figure 7.The results revealed pronounced infiltration of polymorphonuclear leukocyte (PMNL) in disease control group mice at day 7. Vehicle-treated group showed PMNL infiltration, fibroblast proliferation, vascularization, and some epithelialization (day 7, vehicle control), whereas mice treated with Lp2621 and betadine showed significant recovery of wound as indicated by mild PMNL infiltration, marked fibroblast proliferation, vascularization, and a distinct and thicker epidermis at day 7. Day 14 data showed the development of capillaries, fibroblasts, collagen, and connective tissue in the treated groups (betadine and Lp2621), whereas fibroblast cell formation, granulation tissue, follicle formation, and finally, re-epithelialization of the wound skin were delayed in the control groups (diseased and treated with vehicle).

. . Immunomodulatory potential of Lp
The immunomodulatory effect of Lp2621 was studied in the serum of mice.Quantification of IL-6 was performed using the BD Enhanced Sensitivity Kit according to the manufacturer's instructions.Levels of IL-6 (pro-inflammatory cytokine) were higher in disease-control mice on day 7 and decreased significantly after treatment with betadine and Lp2621 on days 7 and 14.However, this difference between the vehicle-and betadine-treated groups was not significant at day 7 but was significant at day 14.Interestingly, we observed a significant decrease in the levels of IL-6 in the Lp2621-treated mice compared with the vehicletreated ( * * p = 0.03) mice on both day 7 and day 14 (Figure 8).However, we could observe IL-10 expression only in a few samples of the betadine and Lp2621-treated groups of mice at day 14.Therefore, no conclusion can be drawn from this observation (data not shown).

Discussion
The present study was envisaged to study the ability of Lp2621, CFS from L. plantarum, to inhibit MRSA infection.Furthermore, the free radical scavenging activity (measured by the DPPH method), immunomodulatory analysis, and healing of excision wounds in mice models infected with MRSA were also established.Earlier studies have shown that L. plantarum MTCC 2621 and its cell-free supernatant have antibacterial activity against Staphylococcus aureus MTCC 737, Micrococcus luteus MTCC 106, Klebsiella pneumonia MTCC 618, Pseudomonas aeruginosa MTCC  1934, Bacillus subtilis MTCC 441, and Escherichia coli MTCC 739 (Sreevani and Kumari, 2013;Dubey et al., 2021).This prompted us to investigate the anti-MRSA role of Lp2621.We observed significant antibacterial activity of Lp2621 against the clinical strain MRSA 831 using an agar gel diffusion assay.Further, we were interested in checking the anti-biofilm activity of Lp2621 because biofilm helps these bugs in their survival against many antibiotics.
Biofilms are aggregates of complicated, sessile populations of microorganisms that are either firmly rooted in an extracellular matrix or attached to a surface.As a result, the bacteria are resistant to adverse environments and antibacterial treatments, making effective treatment extremely difficult (Roy et al., 2018).Lactic acid bacteria (LAB) and their metabolites, viz.exopolysaccharides, enzymes, peptides, polyphenols, antibiotics, and lantibiotics-nisin , and ****p < . .
have been investigated as possible biofilm biocontrol agents (Parisot et al., 2008;Roy et al., 2018;Song et al., 2020).Given that Lp2621 exhibited anti-MRSA activity and that MRSA is known to form biofilms, we next used confocal microscopy to test the effectiveness of Lp2621 in preventing and eradicating biofilm.
In the biofilm inhibition assay, cells were exposed to Lp2621 at varying concentrations, including 50, 12.5, and 0.78% v/v, against MRSA 831.The findings revealed that biofilm formation was totally suppressed at these concentrations.Karska-Wysocki et al. (2010) discovered the antibacterial activity of Lactobacillus acidophilus and Lactobacillus casei, which contain antimicrobial components that can limit the growth and destroy MRSA cells.These observations are in agreement with earlier reports utilizing CFS of Lactobacillus brevis KCCM 202399 and L. fermentum TCUESC01 (Melo et al., 2016;Kim et al., 2022).Lp2621 has been shown to limit the amount of biofilm that pathogenic clinical MRSA strains may form, making it a possible candidate for use in the fight against antimicrobial resistance (AMR).Experiments on biofilm eradication have demonstrated how challenging it is to entirely eliminate a biofilm.L. plantarum and its by-products have been shown to possess a high capacity to block the infectioncausing abilities of P. aeruginosa and MRSA (Onbas et al., 2019;Moghadam et al., 2020).Our findings also suggest that Lp2621 has strong biofilm inhibition activity against clinical isolate of MRSA 831 at higher doses (Figure 3).Excellent DPPH scavenging activity was demonstrated by Lp2621, which has previously been noted as the cell-free supernatant of various lactobacilli in the literature (Xing et al., 2015;Sornsenee et al., 2021).Next, we evaluated the immunomodulatory activity of Lp2621 and found that it has the potential to alter the expression of pro-and anti-inflammatory cytokines, according to cytokine gene expression study and quantification by qRT-PCR using THP-1 cells.These results corroborated with previously published studies (Bermudez-Brito et al., 2013;Marco et al., 2018).To further validate the role of Lp2621 in the treatment of wounds infected with MRSA in mice, a gel was prepared having CFS of L. plantarum MTCC 2621.The rheological properties of the gel were evaluated in terms of viscosity, and the gel was found to be suitable for use (non-Newtonian pseudoplastic).The gel showed tremendous healing activity in wounds infected with MRSA.L. plantarum applied to a wound before and after the initiation of MRSA infection reduced the occurrence of MRSA superinfection in a burn wound model in rats (Sürmeli et al., 2019).Previous studies have also shown that probiotics/lactobacilli play a role in healing various types of wounds, such as oral wounds (Han et al., 2019), excisional wounds in Wistar rats (Sinha et al., 2019), chronic ischemic wound lesions (von Ossowski et al., 2013), and burn wounds (Moghadam et al., 2020).
Histopathological analysis of the skin tissue revealed that Lp2621 had an impact on the lesions, as evidenced by PMNL infiltration, fibroblast proliferation, vascularization, and epidermal development.Similar histological alterations, including PMNL infiltration, collagen deposition, angiogenesis, and tissue granulation, were seen in an excisional wound treated with a cell-free supernatant of Bifidobacterium bifidum in conjunction with chitosan (Bazjou et al., 2022).L. rhamnosus, when administered orally to Swiss mice, stimulated epithelization, reduced macrophage and mast cell infiltration, improved angiogenesis and blood circulation, collagen deposition and scarring, and also reduced inflammation and fibrogenesis in wounded skin (Moreira et al., 2021).
Another key finding of our study is that Lp2621 treatment of mice with wounds contaminated with MRSA resulted in a decreased expression of IL-6 in serum.These observations are in agreement with our previous studies, where decreased IL-6 levels were reported in mice with wounds infected by S. aureus following treatment with Lp2621 (Dubey et al., 2021).Probiotic bacterial strains and/lactic acid bacteria have been shown in numerous studies (Karamese et al., 2016;Johnson et al., 2020;Dubey et al., 2021) to reverse histopathological abnormalities in wounds and modify pro-and anti-inflammatory cytokines in serum.These findings are the first tangible proof that Lp2621 promotes wound healing by limiting the growth of the clinical MRSA strain in vivo.
In summary, our results show that Lp2621, a probiotic CFS, has potent antibacterial and antioxidant properties.It also exhibits in vitro biofilm inhibition and eradication activity, as well as anti-MRSA activity.Additionally, it showed promise in the treatment of mice with MRSA-infected wounds.Our findings support the notion that L. plantarum produces metabolites capable of suppressing growth and eradicating MRSA.Although more research is needed to explore the mechanistic insights and characterize the metabolites responsible for these activities, the strategy of using Lp2621 as a therapeutic intervention against MRSA and other antibiotic-resistant microbiological infections can be weighed.
as an inhibitor of MRSA: in vitro and in vivo studies.

FIGURE
FIGUREFlowchart outlining the research plan for examining Lp′ s ability to treat MRSA-infected wounds and to suppress, eliminate, and eradicate biofilms.

%
of Inhibition of the DPPH radical = Abs control − Abs sample − Abs blank Abs control × 100 (3) Abs (control) = The absorbance of DPPH and methanol.Abs (sample) = The absorbance of sample.Abs (blank) = The absorbance of the sample and methanol.

FIGURE
FIGURE The e ect of Lp on biofilm: insights into inhibition and eradication; (A) E ective inhibition of biofilm formation; (B) Eradication of MRSA by Lp ; (C) Confocal laser scanning microscopy (CLSM) -Series , , , and show TD, dye only, merged image of TD and dye, and Z-stack images, respectively.SYTO-( µM) dye was used for observation at a wavelength of and magnification of × (oil).Scale bar: µm.The data shown are from three independent biological experiments (mean ± SD).

FIGURE
FIGURE Percent DPPH inhibition (Lp : to .% v/v) and EC values of DPPH scavenging activity of Lp .Data presented are in triplicate from three independent biological experiments (mean ± SD). ****mean p < ..

FIGURE
FIGUREGene expression analysis (A) IL− and (B) IL− genes in THP-cells treated with di erent concentrations of Lp .Fold change was compared with control (untreated cells) and calculated with respect to GAPDH (glyceraldehyde--phosphate dehydrogenase).Data are representative of three independent experiments in triplicate, expressed as mean ± SD.

FIGURE
FIGURERheological profile of Lp gel (A) and its e ect on wound healing; (B) percent wound contraction in wounds of di erent groups of mice; and (C) representative images of wound healing in mice.Data are presented as mean ± SD. *p = ., **p = ., and ****p < . .

FIGURE
FIGUREImmunomodulatory activities of Lp.Analysis of cytokine immune response profile in response to Lp treatment in serum of mice.Data are expressed as mean ± SD. *p = .and **p = . .