Current Advances on Structure-Function Relationships of Pyridoxal 5′-Phosphate-Dependent Enzymes

Pyridoxal 5′-phosphate (PLP) functions as a coenzyme in many enzymatic processes, including decarboxylation, deamination, transamination, racemization, and others. Enzymes, requiring PLP, are commonly termed PLP-dependent enzymes, and they are widely involved in crucial cellular metabolic pathways in most of (if not all) living organisms. The chemical mechanisms for PLP-mediated reactions have been well elaborated and accepted with an emphasis on the pure chemical steps, but how the chemical steps are processed by enzymes, especially by functions of active site residues, are not fully elucidated. Furthermore, the specific mechanism of an enzyme in relation to the one for a similar class of enzymes seems scarcely described or discussed. This discussion aims to link the specific mechanism described for the individual enzyme to the same types of enzymes from different species with aminotransferases, decarboxylases, racemase, aldolase, cystathionine β-synthase, aromatic phenylacetaldehyde synthase, et al. as models. The structural factors that contribute to the reaction mechanisms, particularly active site residues critical for dictating the reaction specificity, are summarized in this review.


INTRODUCTION
Pyridoxal 5 ′ -phosphate (PLP) is one of the active forms of vitamin B 6 , which is produced by pyridoxal kinase-mediated reactions. PLP-dependent enzymes catalyze a wide variety of reaction types and usually have a conserved lysine residue in the active site for PLP binding. The ε-amino group of the lysine residue and the aldehyde group of PLP forms a Schiff-base structure. Because this Schiff-base structure is linked through a protein-associated lysine residue, it is commonly referred as internal aldimine. After substrate (amino acid or amine) binding, the internal aldimine breaks up and a new Schiff base structure is formed between the amino group of substrate and aldehyde group of PLP via a gem-diamine intermediate (Fukui and Soda, 2008). This newly formed Schiff base is generally termed external aldimine to distinguish it from Schiff-base structure linked with the lysine residue in proteins. The aldimine exchange has been termed transaldimination. This external aldimine formation is common in many PLP-containing enzymes, but once external aldimine is formed, the subsequent reaction mediated by any given enzyme differs, which is dictated primarily by active site conformation or more specifically by the biochemical characteristics of the active site residues that interact with the specific chemical groups of the external aldimine.
In addition to the diversity of reaction specificity, PLPdependent enzymes are involved in many key cellular processes and metabolism. These enzymes are involved in the amino acid metabolism and production of amino acid-derived metabolites. For example, L-3,4-dihydroxyphenylalanine (Ldopa) decarboxylase (DDC) catalyzes the decarboxylation of L-dopa and 5-hydroxytryptophan to produce dopamine and serotonin, respectively. Both dopamine and serotonin are neurotransmitters for not only mammals but also many other species. The regulation problems and deficiency of PLP-dependent enzymes caused probably by pathogenic mutations, lead to several metabolism symptoms. For example, the deficiency of DDC leads to development delay, abnormal movement, and other neurotransmitter-related symptoms. Deficiency of alanine-glyoxylate aminotransferase is involved in the primary hyperoxaluria type I disease while ornithine aminotransferase deficiency contributes to vision problems at night or under dim light (Cellini et al., 2014). There is also an inverse association between the blood PLP level and the risk of colorectal cancer (Larsson et al., 2010). In addition, it was reported and evaluated recently, for the first time that the insufficient PLP or B 6 intake from food might contribute to pancreatic islet autoimmunity and the development of type I diabetes (Rubí, 2012).
As a result of diverse reaction specificity and the physiological significance, PLP-dependent enzymes are the foci of enzyme structure-function relationship studies. With the technological and methodological development, especially the sequencing of more genomes, technical progress of protein expression and purification, and the determination of more enzyme crystal structures, a better understanding of enzymatic catalysis is achieved, especially how the active site residues facilitate PLP to increase the specific reaction and at the same time decrease the possibility of side reactions. In this review, we focus on mechanisms involved in initial catalytic steps shared by most PLP-dependent enzymes and try to link the mechanism of one enzyme to the reaction specificity mechanism of enzymes with similar reaction specificity from different species.

NEW INSIGHTS INTO PLP CHEMISTRY IN CATALYSIS
PLP acts as a cofactor in these enzymes because of its specific properties. PLP has one heteroaromatic pyridine ring, one aldehyde group, a hydroxyl group, and a phosphate group.
The aldehyde group of PLP makes it possible to form imine with free amino group (e.g., the internal aldimine, formed between PLP and the conserved lysine residue and external aldimine formed between PLP and substrate amino group). PLP binding in the active site through phosphate group in some exceptions are also observed (Eliot and Kirsch, 2004). Another unprotonated primary amino group can attack the internal aldimine or external aldimine formed to make the internal or external aldimine reversible. The ability to form reversible imine allows PLP binding, substrate binding, product release and regeneration of enzyme with PLP bond in the active site.
The heteroaromatic pyridine ring of PLP enables it to stabilize carbanionic intermediate formed for most (if not all) of the PLP-dependent enzymes, except aminomutase family (radicalinitiated reaction) (Frey, 2001). The electrons of the carbanionic intermediate are resonance stabilized and delocalized by the electron-sink of PLP. Quinonoid intermediates, proposed as the key intermediates in many PLP-dependent catalytic mechanisms, are the resonance forms of carbanionic intermediates, but quinonoid intermediates have not been actually observed in some reactions, such as, the reaction catalyzed by alanine racemase. In alanine racemase, the unprotonated pyridine nitrogen made it difficult to form a quinonoid intermediate, which might be destabilized and could be a transition state in the reaction catalyzed by alanine racemase (Major and Gao, 2006).
The functions of other groups of PLP were also discussed. The hydroxyl group could function as a proton donor or acceptor, which will be discussed in detail in the mechanism part. The role of 5 ′ -phosphate group was less mentioned or elaborated. It was proposed that the phosphate group of PLP played a role as a general acid/base for accepting or donating a proton in the reaction catalyzed by glycogen phosphorylase (Helmreich, 1992;Livanova et al., 2002). The phosphate group of PLP is in close proximity to the substrate phosphates (Parrish et al., 1977;Chang et al., 1983;Livanova et al., 2002). In the forward reaction, the glycoside oxygen is protonated by orthophosphate and PLP becomes a dianion by donating one proton to the substrate phosphate. Then a covalent ligation of substrate phosphate with the intermediate leads to glucose 1phosphate formation and PLP is converted back to a monoanion (Helmreich, 1992;Livanova et al., 2002). The importance of the phosphate group in PLP was also studied recently through comparative analysis using pyridoxal and pyridoxal 5 ′ -phosphate as the cofactor in serine palmitoyltransferase. Although pyridoxal could associate with a conserved active site lysine residue and transaldimination reactions proceeded, a replacement of PLP with pyridoxal lowered more than 10-fold of its enzyme activity (Beattie et al., 2013). The phosphate group of PLP was proposed to interact with the substrate L-serine hydroxyl group and contributed to the critical intermediate formation and stereospecific orientation of formed quinonoid or carbanionic intermediates (Beattie et al., 2013). The phosphate group was also suggested functioning as an acid/base catalyst to promote proton transfer to aid in external aldimine formation and accelerating gem-diol intermediate formation in kynureninasemediated hydrolytic cleavage reaction (Phillips et al., 2014).
is through deprotonation on one side and reprotonation of Cα on the opposite side, while a reprotonation at C4 ′ of PLP following deprotonation at Cα is a critical step of transamination for a ketimine intermediate formation. The α, β-elimination is dependent on the leaving group at the β position.
Decarboxylation is through the removal of -COO − group from the external aldimine to form a carbanionic or quinonoid intermediate and followed by protonation of the intermediate at Cα to form an amine. Retro-aldol condensation is through bond breaking between Cα and Cβ to form a carbanionic intermediate (Toney, 2011). Some enzymes catalyze a combination of different reaction types, e.g., dialkylglycine decarboxylase (DGD), which catalyzes the decarboxylation-dependent transamination (Fogle and Toney, 2010;Taylor et al., 2015).

FOLD TYPES OF PLP-DEPENDENT ENZYMES
Grishin et al. classified PLP-dependent enzymes into 5-fold types and pointed out that some enzyme families could not be categorized into these 5-fold types and might be arranged to additional fold types (Grishin et al., 1995). Subsequently, PLP-containing proteins were separated into seven clusters (Percudani and Peracchi, 2009), which contained the 5-fold types suggested by Grishin et al. (1995) and two additional clusters assigned as fold types VI (including D-lysine-5,6-aminomutase) and VII (containing lysine 2,3-aminomutase) (Percudani and Peracchi, 2009).
Fold type I includes aminotransferases (except aminotransferase class IV), decarboxylase groups II and III (Grishin et al., 1995;Steffen-Munsberg et al., 2015) and some enzymes with α-, β-or γ-elimination activity (Kappes et al., 2011). These fold type I enzymes usually have their Schiff base Lys residue near C-terminus, a glycine-rich loop (involved in binding of PLP phosphate group) and a hydrophobic β-strand before the Lys residue. This fold group usually contains a conserved aspartate residue that interacts with the PLP ring N atom and this residue is 20-50 amino acids preceding the Lys residue (Grishin et al., 1995). Fold type I enzymes usually exist as homodimers or homotetramers and each subunit contains a PLP molecule, but their active site is located at the interface between subunits and is composed of residues from two subunits (most residues are from one subunit) at the interface (Schneider et al., 2000;Han et al., 2010b;Milano et al., 2013). Although the dimer is typically the minimum assembly required for catalytic activity, the active site of Escherichia coli L-threonine aldolase tetramer containing residues from three monomers was also reported (Di Salvo et al., 2014). The subunit of fold type I enzymes contains a large domain and a small domain. The large domain consists of a seven-stranded β-sheet at the N-terminal. The small domain (the C-terminal of enzymes) folds into a 3-or 4-stranded β-sheet covered with helices on one side (Schneider et al., 2000;Han et al., 2010b). Structural alignment further classified the fold type I enzymes into six subclasses according to the structure of the N-terminal part (Mehta et al., 1993;Grishin et al., 1995;Käck et al., 1999;Schneider et al., 2000).
Tryptophan synthase β-family is the representative enzyme of fold type II. Other enzymes (e.g., cysteine synthase, serine dehydratase, threonine dehydratase, O-acetyl serine sulfhydrylase, threonine synthase, etc.) also belong to fold type II (Grishin et al., 1995). Fold type II enzymes, in contrast with fold type I enzymes, usually have the Schiff base Lys residue closer to N-terminus, while the loop region for PLP phosphate group binding is near the C-terminus. There are two β-strands flanked by α-helices in fold type II enzymes. Glu350 in tryptophan synthase is involved in PLP binding and Asp or Ser are also conserved at the equivalent position suggested by sequence alignment of fold type II enzymes (Percudani and Peracchi, 2009). Different from fold type I to have one Asp residue to interact with the pyridine nitrogen, a serine residue is conserved in fold type II enzymes at the equivalent position (Schneider et al., 2000). Fold type II enzymes are functional as dimers, tetramers, or oligomers with the active site being composed of residues all from one subunit (Milano et al., 2013) and many fold type II enzymes are under allosteric control. Tryptophan synthase exists as an α 2 β 2 tetramer with α and β being the regulatory subunit and the catalytic subunit, respectively. Threonine deaminase is a homodimer and has one catalytic domain and one regulatory domain in each subunit. The region for PLP-binding in fold type II enzymes contains an N-terminal domain and a C-terminal domain with similar size to each other (Schneider et al., 2000).
Fold type III enzymes (e.g., alanine racemase and eukaryotic ornithine decarboxylase) have similar arrangement for aldimineforming Lys residue (closer to N-terminus) and PLP interacting glycine-rich loop (near C-terminus) as those of fold type II enzymes, but fold type III enzymes have a hydrophobic βstrand before the conserved Lys residue and two β-strands in the region of β/α units (Grishin et al., 1995). Alanine racemase and human ornithine decarboxylase are active as dimers. Alanine racemase consists of two domains in each subunit. One domain is composed of eight-stranded α/β barrel and another one contains β strands. In alanine racemase, PLP binding site is in a cleft between these two domains and one arginine residue interacts with the pyridine ring nitrogen atom through forming a hydrogen bond (Schneider et al., 2000). In mammalian ornithine decarboxylases, a glutamic acid residue is at the equivalent position interacting with the pyridine ring nitrogen atom (Kern et al., 1999). D-alanine aminotransferase family and branched chain aminotransferase were assigned as fold type IV enzymes (Grishin et al., 1995;Okada et al., 1997;Percudani and Peracchi, 2009;Kappes et al., 2011). The fold type IV enzymes are usually functional as homodimers, and branched-chain aminotransferase is a hexamer. There are two domains. The Nterminal domain includes a six-stranded antiparallel β sheet and two α helices. The C-terminal domain consists of a pseudo-βbarrel and some helices. A glutamate acid residue interacts with the pyridine ring nitrogen atom (Schneider et al., 2000).
The glycogen phosphorylase family was classified as fold type V enzyme, which contains a PLP-binding domain with a lactate dehydrogenase fold (Grishin et al., 1995). For fold type V enzymes, PLP phosphate group is involved in proton transfer in the enzymatic reaction (Schneider et al., 2000). Different from other fold types listed above, glycogen phosphorylase has no hydrogen bond interactions between the active site residues and the pyridine ring nitrogen atom of PLP (Schneider et al., 2000). Glycogen phosphorylase has two forms (phosphorylase a and b) and exists as tetramer or dimer. The conversion from phosphorylase b to a is through phosphorylation of phosphorylase protomer b catalyzed by phosphorylase b kinase while the reverse reaction is mediated by phosphorylase phosphatase (Cohen et al., 1971). A study on phosphorylase a indicated that both dimer and tetramer enzymes could bind glycogen, but the tetramer bound the substrate with lower affinity and had no activity. The glycogen binding lowers the association rate of the tetramer formation from dimer, but the dissociation of tetramer to form dimer is not affected by glycogen binding. The existence of both dimer and tetramer form of glycogen phosphorylase might be involved in a regulation mechanism for the glycogen metabolism (Wang, 1999). There are three domains in glycogen phosphorylase, including an Nterminal domain, a glycogen-binding domain, and a C-terminal domain (Schneider et al., 2000).
The enzymes reviewed in this study belong to fold type I to IV. Enzymes catalyzing a reaction type include enzymes from different fold types and a fold type is composed of enzymes catalyzing diverse kinds of reactions. For example, aminotransferases belong to the fold type I and IV, and alanine racemase and serine racemase belong to fold type III and II, respectively (Watanabe et al., 2002;Canu et al., 2014). Both aromatic amino acid decarboxylase and fruit fly phenylacetaldehyde synthase are fold type I enzymes. This review focuses on residues involved in reaction specificity of structurally and mechanistically related enzymes. Serine hydroxymethyltransferase, L-threonine aldolase, and fugal alanine racemase are fold type I enzymes and have similar active site residues and all have half-transamination, aldol cleavage, and racemization activity (Contestabile et al., 2001;Di Salvo et al., 2013, 2014. Aromatic amino acid decarboxylase and phenylacetaldehyde synthase may share similar reaction steps from external aldimine formation, decarboxylation, to the carbanionic intermediate formation, but deviate from each other by the different ability to protonate the carbanionic intermediate at Cα by varied active site residues (Liang et al., 2017). In addition to protonation efficiency differences, the deprotonation and protonation stereo-specificity are observed between racemase and aminotransferase. Racemases catalyze deprotonation at Cα of the substrate on one side and reprotonation on the another side at Cα while deprotonation at Cα of substrate and reprotonation at C4 ′ of PLP are the key steps in reactions catalyzed by aminotransferase (Eliot and Kirsch, 2004;Toney, 2011). Cystathionine β-synthase is a member of fold type II (Miles and Kraus, 2004) and catalyzes βreplacement reactions. Similar to reactions catalyzed by racemase and aminotransferase, the first step of reactions catalyzed by cystathionine β-synthase is proton abstraction from Cα. These functionally and mechanistically related enzymes are reviewed here (Koutmos et al., 2010).

Stereoelectronic Effects: Unifying Enzymatic Mechanism
The structural characteristics of PLP make it possible to undergo a number of reactions. Its pyridine ring could function as an electron sink for delocalization and contribute to the resonance stabilization of the electrons or negative charges developed from bond breaking at Cα. However, in a given buffer, mixing of PLP and amino acid or amine does not lead to detectable specific reactions as compared with reactions catalyzed by PLP-dependent enzymes (the rate of reaction is too slow to be physiologically relevant). Only the combination of given protein component and PLP makes the PLP-dependent enzymes work efficiently on the specific substrate and specific reaction direction. One might ask how a given enzyme controls the reaction specificity because PLP functions in most PLPdependent reactions to delocalize and stabilize the electrons or negative charge (developed from bond breaking at Cα) in the transition state.
In 1966, Dunathan proposed a unified theory explaining the reaction specificity of PLP-dependent enzymes (Dunathan, 1966). It was hypothesized that, if the delocalization energy was gained after the loss of one group from Cα, the PLP-dependent enzyme should bind the substrates in a specific geometry to have the bond to be broken in a perpendicular plane to the plane defined by pyridoxal imine (the π system) to make the bond labile to be broken (Dunathan, 1966). For example, the αdecarboxylase was predicted to bind the substrates in a specific orientation to have the -COO − group perpendicular to the plane of Schiff base and PLP ring. This orientation was proposed to facilitate decarboxylation process.

External Aldimine Formation
After substrate binding, the incoming substrate interacts with the internal aldimine through forming an unstable gem-diamine intermediate, and that leads to breaking up of internal aldimine and formation of external aldimine. This process is commonly termed transaldimination.
It has been generally considered that a nucleophilic attack of the substrate amino group at C-4' of the internal aldimine was the first step during transaldimination. To promote this reaction, it was proposed that the aldimine nitrogen should be protonated and this topic has been discussed in detail (Spies and Toney, 2003;Chan-Huot et al., 2013). In addition to the aldimine nitrogen, some recent structural analyses of a PLPcontaining tyrosine phenol-lyase suggested that the interactions of aspartate residue and pyridine ring N atom might play a role in favoring transaldimination. The site-directed mutagenesis of Asp214 to Ala led to deprotonation of the pyridine ring N, the reduced reaction activity, one assumed relaxed geometry and the increased stability of the internal aldimine. It was proposed that this observed stabilization of the ground state in D214A variant might reduce the rate of the external aldimine formation, while Asp214 in wild-type enzyme might maintain the strained state of the internal aldimine, which likely led to the acceleration of transaldimination (Milić et al., 2012). The Asp residue is usually conserved, but in some enzymes, this residue is replaced by a Glu, which can interact with PLP pyridine ring N in the same manner (forming salt bridge) in fold type I and IV enzymes. In fold type II enzymes, the PLP pyridine ring N also interacts with Ser or Thr or Cys through forming hydrogen bonds. Whether these residues also have a role in different fold type enzymes as the Asp does in favoring transaldimination needs further elucidation.
To proceed nucleophilic attack on PLP C4 ′ atom, the substrate amino group must be a neutral group (not positively charged) in order to function as a nucleophile. In a reaction mixture under the physiological condition (pH around 7), the substrate amino group is usually protonated according to the pKa of the amino group. This leads to another essential question as to how the amino group of a substrate is deprotonated. A conformational study of one PLP-dependent enzyme suggested that nucleophilic attack of the substrate was achieved by a molecular switch of the dihedral angle between pyridine ring and Schiff-base linkage. PLP O3 ′ functions as a proton acceptor to make the nucleophile available (Ngo et al., 2014). Whether this is the typical way or mechanism for deprotonation of substrate amino group in other PLP-dependent enzymes needs to be analyzed.

Aminotransferase
Aminotransferase catalyzes the reversible transformation between an amino acid and α-keto acid. After external aldimine formation, deprotonation at Cα leads to a carbanionic intermediate or quinonoid intermediate. Reprotonation at C4 ′ position of PLP leads to a ketimine intermediate and one H 2 O molecule is added to the intermediate Cα. The half-reaction proceeds to have α-keto acid involved to regenerate PLP and another amino acid. This is a commonly accepted reaction mechanism. In the process, proton transfer between Cα and C4 ′ was suggested to be promoted by the conserved Lys residue, which is involved in the formation of internal aldimine. The role Lys residue plays in proton transfer was supported by a greatly decreased rate of catalysis in enzyme variant (Toney and Kirsch, 1993). In literature, there has been a debating issue on the concerted or stepwise mechanism of proton transfer between Cα and C4 ′ (Goldberg and Kirsch, 1996;Toney, 2014). The existence of quinonoid intermediate was indicated by light enhancement of aspartate aminotransferase-catalyzed reaction and also by low concentrations of quinonoid intermediate in steady state (Toney, 2014).
Aminotransferases have an uncharged aromatic amino acid residue (Phe or Tyr or Trp, depending on different aminotransferases) for π-π stacking of the PLP ring (Figure 2). The presence of uncharged aromatic amino acid residue (Trp, Tyr, and Phe) for π-π stacking likely enhances the proton transfer opportunity between Cα and C4 ′ through Lys residue by leaving more lifetime for the intermediate to allow proton delivery by Lys residue.
In addition to uncharged aromatic amino acid for π-π stacking with PLP, there are two active site residues Asn and Tyr that are conserved in aminotransferases (Figure 2) and form hydrogen bonds with PLP phenol group O3 ′ (Figure 2) (Sivaraman et al., 2001;Han et al., 2008aHan et al., ,b, 2010aDuff et al., 2012;Nasir et al., 2016) (http://www.rcsb.org/structure/3DYD). By analyzing the catalytic mechanism, we propose that although ionized forms of phenol group could also possibly exist (Figure 3, form I and II), the neutral forms are likely predominant in the catalysis because ionized O3 ′ has more lone pairs of electrons (Figure 3, form I) than that of the neutral form (Figure 3, form III) and needs to be delocalized into the pyridine ring for stabilization (Figure 3, I and II). The delocalization effect of more lone pair electrons of ionized O3 ′ may counteract with the electron delocalization effect of reaction intermediates. These two residues likely help most of O3 ′ in neutral 3 ′ -OH form. The neutral 3 ′ -OH group, stabilized by hydrogen bonds with neutral Tyr and Asn, may better facilitate carbanionic intermediate formation and delocalization of electrons of the intermediate (Figure 3, form III) and thus may better promote deprotonation of Cα than other ionized form (Figure 3, form III and quinonoid intermediate). The conservation of these two residues for hydrogen bond formation and the predicted role in electron delocalization and intermediate stabilization suggest the importance of the interconverting intermediate stability. This is in agreement with the stepwise mechanism of aminotransferase.
Results derived from neutron crystallography of aspartate aminotransferase suggested that the O3 ′ was deprotonated in both internal aldimine and external aldimine (Dajnowicz et al., 2017). Whether, the O3 ′ is protonated or deprotonated after Cα deprotonation remains to be substantiated. Likewise, the possible role of O3 ′ protonation state in the carbanionic intermediate formation after Cα deprotonation needs further elucidation.
The roles of one flexible Arg residue that interacts with the carboxylate group of substrates in aminotransferases were also reported. ω-aminotransferase is active on multiple substrates, e.g., hydrophobic amines and amino acids. A density functional calculation study on Chromobacterium violaceum ω-aminotransferase suggested that the conformation (pointing its side chain toward or away from the active site) of Arg residue was a switch of recognition between the dual substrates (amino acids or hydrophobic amines). The reaction mechanisms of deamination for both substrates were similar, but reaction energetics was quite different. With the side chain of Arg416 positioned inside the active site, the half-transamination reaction from alanine to pyruvate was able to proceed because Arg416 aided in alanine binding through a salt bridge interaction with the carboxylate group of substrates. With the (S)-phenylethylamine as the substrate, the side chain of Arg416 pointed away from the active site (Manta et al., 2017). Similar roles of Arg residues were also reported in other aminotransferases. Escherichia coli aspartate aminotransferase has Arg292 and Arg386 to interact with the dicarboxylic substrates. When the side chain of Arg292 was pointing toward the active site, the enzyme FIGURE 2 | Partial protein sequence alignment of aminotransferases, and the position of three conserved residues in the active site. Sequences of Homo sapiens kynurenine aminotransferase (Accession: NP_057312.1), Mycobacterium tuberculosis H37Rv phenylalanine aminotransferase (PDB: 4R2N, chain A) (Nasir et al., 2016), Enterobacteriaceae L-histidinol phosphate aminotransferase (Accession: WP_000108941.1), Homo sapiens tyrosine aminotransferase (PDB: 3DYD, chain A) (http://www.rcsb.org/structure/3DYD) and Hordeum vulgare alanine aminotransferase (PDB: 3TCM, chain A) (Duff et al., 2012) are shown. The uncharged aromatic amino acid residues for π-π stacking with PLP ring are highlighted in blue box while the Asn and Tyr residues forming hydrogen bonds with PLP O3 ′ are highlighted in red boxes (A). The Asn and Tyr residues forming hydrogen bonds with O3 ′ of PLP are shown in green sticks while the ligands pyridoxamine phosphate (PMP), L-kynurenine and D-pyridoxyl-N,O-cycloserylamide-5-monophosphate (DCM) are shown in blue sticks. The uncharged aromatic amino acid residues forming π-π stacking with PLP pyridine ring and the Asp residue near pyridine ring N atom are also shown in green sticks. The distances between PLP O3 ′ and Asn or Tyr and the distances between pyridine ring N and Asp side chain are in blue dashed line and labeled (Unit: Å). L-kynurenine aminotransferase (PDB: 2R2N) (Han et al., 2008b)  had a higher binding affinity for dicarboxylic products and substrates. Arg386 interacts with the carboxylate group of aromatic substrates and dicarboxylic substrates (Chow et al., 2004). Arg414, which was the only arginine residue in the active site of Paracoccus denitrificans ω-aminotransferase, was found to recognize carboxylate group of substrates (Park et al., 2012). Arg417 was at the equivalent in a class III aminotransferase (PDB: 3HMU) from Silicibacter pomeroyi and was proposed to be involved in substrate promiscuity because of its flexibility (Rausch et al., 2012).

PLP-Dependent Aromatic Amino Acid Decarboxylase
In PLP-dependent decarboxylase, PLP serves as an electron sink to delocalize the unbounded electrons and to orientate enzymesubstrate in a specific orientation, both of which contribute to the decarboxylation and formation of a quinonoid intermediate. The protonation at Cα leads to an imine formation, which is attacked by Lys amino group to lead to one Schiff base formation between Lys residue and the PLP (internal aldimine). At the same time, the amine product is released. This is the generally accepted mechanism for the decarboxylation process. In the decarboxylation pathway, production of the quinonoid intermediate is reasonable and well accepted. The protonation of quinonoid intermediate at Cα is necessary after decarboxylation and the residues or other factors that promote protonation of Cα have not been determined (at least not clearly specified). Our recent study on 3,4-dihydroxyphenylalanine decarboxylase (DDC) identified that His192 was the residue that protonated quinonoid intermediate after decarboxylation (Liang et al., 2017). This active site histidine residue is stringently conserved in all PLP-dependent aromatic amino acid decarboxylases (Figure 4) and in close proximity with PLP ring and Schiff base structure (Figure 4). After decarboxylation, the carbanion intermediate or quinonoid intermediate (resonance structure of carbanion) is stabilized by the PLP electron sink through electron delocalization. The protonation of Cα by His192 promotes electron shift of the quinonoid intermediate and formation of a new double bond between N atom and C4 ′ . After protonation, the intermediate undergoes transaldimination with the ε-NH 3 group of active site Lys residue to release dopamine and regenerate PLP-enzyme complex (internal aldimine). The high efficiency of His192 on promoting protonation of Cα is likely the most important step for the intermediates going through the typical DDC-mediated process (Figure 5) (Liang et al., 2017). Mutation of His192 to asparagine resulted in a great decrease in its decarboxylase activity (Liang et al., 2017), which supports its proposed role in decarboxylase reactions. The H192N mutation of DDC decreased V max (285 nmol min −1 mg −1 ) of dopamine formation around 11-fold compared with that of the wild-type enzymes (3,357 nmol min −1 mg −1 ) (Liang et al., 2017). H192W mutation also lowered the catalytic efficiency of decarboxylation for both 3,4-dihydroxyphenylalanine and 5-hydroxytryptophan (from 128.2 to 21.2 min −1 mM −1 for 3,4-dihydroxyphenylalanine, from 157 to 29.4 min −1 mM −1 for 5-hydroxytryptophan) (Han et al., 2010b).
Other residues involved in substrate binding were also analyzed. In pig kidney DDC complex structure, Thr82 interacts with the 4-catecholhydroxyl group of the carbidopa (a substrate-like inhibitor) (Burkhard et al., 2001). Structural alignment of pig kidney and Drosophila melanogaster 3,4-dihydroxyphenylalanine decarboxylases and Drosophila melanogaster tyrosine decarboxylase 1 and tyrosine decarboxylase 2 indicated that at the equivalent position with Thr82, Thr was found in pig kidney and Drosophila melanogaster 3,4-dihydroxyphenylalanine decarboxylase while Drosophila melanogaster tyrosine decarboxylase 1 and tyrosine 2 had a Ser and Ala residue at the equivalent position (Han et al., 2010b). Thr82 appeared to be a potential residue involved in 3,4-dihydroxyphenylalanine binding while Ser or Ala at the equivalent position might be a possible residue for recognizing tyrosine binding. Site-directed mutation of Thr82 to Ser or Ala increased the K m (K m of T82A = 5.6 mM, K m of T82S = 4.5 mM) toward 3,4-dihydroxyphenylalanine by more than 2-folds compared with that of the wild-type enzyme (K m = 2.2 mM). The T82A variant also increased K m for 5-hydroxytryptophan from 0.4 to 3.5 mM. The increased K m values suggested that the binding affinity was lowered by mutation and Thr82 was involved in substrate binding. However, T82S or T82A mutation did not allow the enzyme to be active on tyrosine as speculated from structural alignment result and Thr82 is not the residue to determine the substrate selectivity toward 3,4-dihydroxyphenylalanine rather than tyrosine (Han et al., 2010b).
From that sequence alignment result (Koyanagi et al., 2012), we also found the critical residue His192 (residue number is from Drosophila melanogaster) (Liang et al., 2017) was conserved at the equivalent position in this bacteria decarboxylase (Koyanagi et al., 2012) and other aromatic amino acid decarboxylases. However, the residues involved in different substrate specificity of aromatic amino acid decarboxylases have not been fully elucidated.

Racemase
Racemases catalyze deprotonation of Cα to form a carbanionic intermediate and then reprotonation of Cα occurs on either reface or si-face of the intermediate to make the stereochemical inversion of molecules in both directions. Racemases can be divided into two classes, PLP-dependent amino acid racemases and PLP-independent racemases (Conti et al., 2011). PLPdependent racemases include serine racemase, alanine racemase and aspartate racemase in eukaryotes and alanine racemase, serine racemase and arginine racemase in bacteria.
For alanine racemase, it has been proposed that Tyr265 was the residue functioning in deprotonation at Cα of L-alanine. The Tyr265 side chain was predicted to be the ionic form when the racemase-catalyzed reaction was under the most active condition (pH 7-9) (Watanabe et al., 1999;Spies and Toney, 2003;Spies et al., 2004;Strych et al., 2007;Yoshimura and Goto, 2008). The theoretical model of Ondrechen et al. also seemed to support the role of Tyr265 in deprotonation (Ondrechen et al., 2001). The Schiff base Lys39 residue functioned to reprotonate the intermediate for the conversion from L-alanine to D-alanine (Figure 6) (Watanabe et al., 1999;Strych et al., 2007;Yoshimura and Goto, 2008). For conversion from D-alanine to L-alanine, Lys39 functioned to deprotonate and Tyr265 reprotonated. Based on these results, the pKa of the external aldimine was estimated to be around 9, which was between pKa values of Lys39 and Tyr265 (Spies and Toney, 2003;Spies et al., 2004).
The role of Tyr265 residue as a general acid-base for L-to Dalanine or D-to L-alanine conversion (Watanabe et al., 1999;Spies and Toney, 2003;Spies et al., 2004;Strych et al., 2007;Yoshimura and Goto, 2008) was reported to need a nearby His166 residue to form hydrogen bond and lower the pKa of Tyr265 side chain (Seebeck and Hilvert, 2003). Seebeck and Hilvert suggested that the Tyr265 to Ala mutation of Geobacillus 1 | Kinetic parameters of Pseudomonas putida and mammalian aromatic amino acid decarboxylases (AAAD) and insect 3,4-dihydroxyphenylalanine decarboxylase (DDC) for the two natural substrates L-3,4-dihydroxyphenylalanine (L-dopa) and 5-hydroxytryptophan (5-HTP).

AAAD or DDC from different organisms
Substrates K m (mM) k cat (s −1 ) k cat /K m (mM −1 s −1 ) References FIGURE 6 | Reaction mechanisms of alanine racemase and serine racemase (Watanabe et al., 1999(Watanabe et al., , 2002Yoshimura and Goto, 2008;Goto et al., 2009). stearothermophilus alanine racemase generated new aldolase activity while the racemization activity was largely reduced. Simultaneously, the substrate selectivity was also changed and the Y265A mutation of alanine racemase allowed the enzyme to be active on D-β-hydroxy-α-amino acid because Tyr to alanine mutation made the active site bigger. The single mutation of the alanine racemase with generated aldolase activity and changed substrate specificity is a good example of how PLP-dependent reaction specificity is achieved through crucial active site residue (Seebeck and Hilvert, 2003). For serine racemase, Lys57 and Ser82 were proposed to be the residues functioning as a general acid-base in the reversible reaction catalyzed by Schizosaccharomyces pombe serine (PDB: 1WTC) (Figure 6) (Goto et al., 2009). Lys56 and Ser84 were reported to be at the equivalent position with yeast Lys57 and Ser82 in human and rat serine racemase (PDB: 3L6B, 3L6R, 3L6C, and 3HMK) . Compared with the Lys residue, Ser82 needs a pKa low enough to function as a general acidbase. A recent study on human serine racemase suggested that Lys114 and one water molecule might work together to lower pKa of Ser84 through a hydrogen bond network among the Ser84, Lys114, and a water molecule (Nelson et al., 2017).
It was also reported that a conformational change of the enzyme contributed to the proper orientation of the Ser residue to function as a general acid/base. After substrate binding, a conformational change of the small domain to close the active site was observed in Schizosaccharomyces pombe serine racemase (Goto et al., 2009). Some structural analyses of mammalian serine racemase identified a small domain movement induced by the substrate or inhibitor binding to close the active site. Intriguingly, the shift of the small domain toward the active site contributed to the specific orientation of Ser84 residue (at the equivalent position with Ser82 in PDB: 1WTC) for the reprotonation step to convert L-serine to D-serine .
Nitoker and Major performed the multiscale quantumclassical simulations and analyzed the free energy profile of the racemization catalyzed by human and rat serine racemase in both gas phase and aqueous solution. Their results suggested a stepwise reaction mechanism of serine racemase, which was similar to that of alanine racemase. Their analyses indicated that it was the solvation effects and the long-range electrostatic interactions with the active site residues that mainly stabilized the intermediate formed during reaction. This conclusion was evidenced by the free energy profile that the deprotonation was unfavored in a gas phase while the aqueous solution stabilized the intermediate formed after first step deprotonation. The active site residues Asn86, His87, and Ser83 functioned to lower the electrostatic repulsion between the negative charges generated by deprotonation step and the carboxylate group (Nitoker and Major, 2015). The authors identified that the unprotonated pyridine ring N seemed to be the correct form for the racemization reaction catalyzed by serine racemase because protonation of the pyridine ring N might facilitate α,β-elimination reaction of L-or D-serine to produce ammonia and pyruvate, which was another activity of serine racemase (Nitoker and Major, 2015). To keep the pyridine ring N from being protonated, serine racemase (fold type II) has one Ser residue that forms a neutral hydrogen bond with pyridine ring N (Jhee et al., 1998;Goto et al., 2009) while bacteria alanine racemase (fold type III) has one Arg residue at the equivalent position (Canu et al., 2014). The study on R219E variant of alanine racemase also identified the possibility of side reactions when the pyridine ring N was protonated in the mutated enzyme (Rubinstein and Major, 2010).

PLP-Dependent Aldolase
Aldolase catalyzes the aldol condensation, leading to C-C bond formation in a stereoselective way. Based on the mechanism and substrate specificity, the aldolases include lysinedependent, metal-dependent, and PLP-dependent aldolases (Contestabile et al., 2001;Dean et al., 2007;Falcicchio et al., 2014). PLP-dependent aldolases have been classified into serine hydroxymethyltransferase (catalyzing glycine formation from L-serine, and 5,10-methylene-tetrahydrofolate formation from tetrahydrofolate) (Fujioka, 1969) and threonine aldolases (mediating the reversible reaction for condensation of small amino acids and aldehyde or degradation of threonine, e.g., condensation of glycine and acetaldehyde to threonine or threonine degradation to acetaldehyde and glycine) (Bell and Turner, 1977;Fesko, 2016).
Compared with D-threonine aldolases, there are more Lthreonine aldolases being identified. Co-crystallization (PDB: 1LW4) of Thermotoga maritime L-threonine aldolase with Lallo-Thr and models built with L-Thr suggested that for L-allo-Thr substrate, His83 was the prospective residue serving as a general base in catalysis to accept one proton from the substrate, but for L-Thr cleavage, it was predicted that, instead of His83, the general base was His125 (from another subunit) or a water molecule (Kielkopf and Burley, 2002). The existence of His83 and His125 might allow flexibility using both L-allo-Thr and L-Thr as substrates. Tyr87 was the residue leading to the substrate preference for L-allo-Thr over L-Thr and the residue at position 87 or equivalent was the only residue varied in all threonine aldolases (Tyr or Phe in enzymes with substrate preference for L-allo-Thr) involved in stereospecificity. It was proposed that the larger side chain of residue at position 87 corresponds to a higher preference for the allo isomer (Kielkopf and Burley, 2002). The native and complex structures (PDB: 4LNJ, 4LNM, 4LNL) of Escherichia coli L-threonine aldolase revealed that the substrate binding had little effect on the structure, and His83 and His126 (at equivalent positions with His83 and His125 in Thermotoga maritime L-threonine aldolase) interacted with a water molecule and the substrate hydroxyl group through hydrogen bonds. A single mutation of either residue and double mutation of both greatly reduced the k cat of the enzyme, but none of these mutations could remove all the activity. His83 to Asn or Phe mutation showed no activity unless a high concentration of PLP was added because His83 was involved in PLP stacking. His126 to Asn or Phe mutation actually doubled the k cat with erythro and threo isomers of threonine as substrates. These results suggested that the two histidine residues might not be directly finctioning as a general acid/base. Due to no other active site residues near substrate hydroxyl group to abstract a proton, these authors hypothesized that His83 and His126 might play a role to help with the coordination of one water molecule, and this water molecule was the one functioning as a general base by transferring a proton to the phosphate group through hydrogen bond (Di Salvo et al., 2014). The mutational study of residue at position 87 was also conducted in Escherichia coli L-threonine aldolase (Di Salvo et al., 2014). Different from the proposed role of residue 87 in Thermotoga maritime L-threonine aldolase (Kielkopf and Burley, 2002), Escherichia coli L-threonine aldolase F87A mutation, with a reduced side chain size at position 87, did not change substrate preference. Another threonine aldolase from Pseudomonas aeruginosa, which does not have any substrate preference, has one Asp residue at position 87. However, when Escherichia coli L-threonine Phe87 was mutated to Asp, with reduced side chain bulk, the substrate preference for L-allo-threonine was even increased. Based on these mutations at position 87, Di Salvo et al. believed that it was the overall active site microenvironment, instead of specific residues that affected the substrate preference (Di Salvo et al., 2014). The role of water molecule as a weak base was also hypothesized in the reaction mechanism of D-threonine aldolase. A crystal structure of a D-threonine aldolase from Alcaligenes xylosoxidans showed that this PLP-dependent enzyme had one metal binding in the active site. The authors proposed that this D-threonine aldolase was similar to L-threonine aldolase in having a water molecule to mediate deprotonation. The metal ion bound the substrate β-hydroxy group and might activate and coordinate the group for deprotonation by another conserved residue His193 (Uhl et al., 2015).
In terms of the donor selectivity and substrate binding, although both His83 and His126 residues might not function as general acid/base directly, they were involved in substrate binding and stereospecificity in Escherichia coli L-threonine aldolase. Both H83F and H126F variants increased the stereospecificity toward L-threonine (Di Salvo et al., 2014). In Aeromonas jandaei L-threonine aldolase, the substrate specificity was affected by His85 (at the equivalent position as His83 in Escherichia.coli L-threonine aldolase), Tyr89, His128 (at the equivalent position as His126 in Escherichia coli L-threonine aldolase), Glu90 and Asp126 (Qin et al., 2014).
Threonine aldolases have been known previously accepting glycine as a donor, but analyses of the expressed L-threonine aldolases and D-threonine aldolases identified some threonine aldolases with novel and broader donor specificities. For example, L-threonine aldolases and D-threonine aldolases from Aeromonas jandaei and Pseudomonas sp. respectively, could accept D-Ser, D-Ala, and D-Cys as donors (Fesko et al., 2009). Fesko et al. attempted to analyze residues important for donor specificity of Aeromonas jandaei threonine aldolases. The region Met281 to Arg313 seemed undergoing some conformational changes and was suggested to be involved in substrate specificity and active site flexibility. Arg313 was a residue conserved in all Lthreonine aldolases to contribute to the donor carboxylate group stabilization. Intriguingly, Arg313 showed slight differences of the spatial orientation in various threonine aldolases, suggesting a potential role in affecting the hydrogen bond network between the active site environment and the donor substrate. These interactions were suggested as being critical for the donor substrate specificity through stabilizing the donor in an active orientation for catalysis (Fesko et al., 2015).
For the donor specificity of another group of PLPdependent aldolase, sequence alignment of serine hydroxymethyltransferase and its analog, α-methylserine hydroxymethyltransferases, indicated that Tyr55 and Tyr65 in serine hydroxymethyltransferases, and Thr60 and His70 in α-methylserine hydroxymethyltransferases were residues involved in donor specificity. Mutation of Tyr55 to Thr in serine hydroxymethyltransferase enabled the enzyme to use both D-Ala and D-Ser while the wild-type enzyme accepted glycine and D-Ala, although the activity of wild-type enzyme using D-Ala was hard to be detected due to the side reaction (Shostak and Schirch, 1988;Hernandez et al., 2015).

Cystathionine β-Synthase
Cystathionine β-and γ-synthase are PLP-dependent enzymes involved in H 2 S formation and also two critical enzymes in the trans-sulfuration pathway (Singh et al., 2009). Cystathionine β-synthase catalyzes β-replacement reactions using cysteine or serine and homocysteine as substrates to produce cystathionine and H 2 S or water, respectively (Koutmos et al., 2010).
Characterization of Lactobacillus plantarum cystathionine βsynthase identified that this wild-type enzyme showed both Oacetyl-L-serine-dependent cystathionine β-synthase activity and O-acetyl-L-serine sulfhydrylase activity (formation of L-cysteine from O-acetyl-L-serine and H 2 S) (Matoba et al., 2017). The existence of both activities is probably due to higher similarity in primary sequence and active site conformation with bacteria O-acetyl-L-serine sulfhydrylase than that with cystathionine βsynthase (Hullo et al., 2007). For substrate preference, H 2 S formation catalyzed by the Lactobacillus plantarum cystathionine β-synthase was greatly enhanced by using both L-cysteine and Lhomocysteine as substrates as compared with the H 2 S produced in the absence of L-homocysteine. With the presence of Lhomocysteine, the k cat /K m for H 2 S synthesis was one magnitude higher than that without L-homocysteine. L-cystathionine and Llanthionine were major and minor byproduct, respectively, when H 2 S was formed (Matoba et al., 2017).
Structural analyses and mutation study suggested that conserved Ala70 and Glu223 in the active site were residues important for H 2 S formation. Glu233 has a restricted conformation and regulates the substrate-binding site size by interacting with residues in another domain. Ala70 and Glu223 are conserved in cystathionine β-synthase while Ser and Gly are conserved at the equivalent positions in bacteria type A O-acetyl-L-serine sulfhydrylase. A70S variant seemed to improve the lifetime of aminoacrylate intermediate and inhibited the localization of the nucleophilic atom to the catalytically active position. E223G variant showed a decreased k cat in β-replacement reaction, which might be due to a narrower substrate-binding site inhibiting product releasing in the E233G variant (Matoba et al., 2017). Bacillus anthracis cystathionine β-synthase is active to o-acetylserine, cysteine and homocysteine, but not active to serine. A mutational study of Bacillus anthracis cystathionine β-synthase indicated the importance of conserved Glu220 for enzyme activity to produce H 2 S (Devi et al., 2017).
Other key residues were proposed by the analyses of human cystathionine β-synthase. PLP is anchored in the active site through residue Lys88. The structure of cystathionine βsynthase showed that the Lys88 residue was close to substrate Cα, Schiff base imino nitrogen and C4A atom. The structure of intermediates indicated that Lys88 might have a role in deprotonation of Cα as a general base to activate its substrate. It was postulated that Lys88 contributed to the stabilization of carbanionic intermediate through electrostatic interactions between the ε-NH + 3 of Lys88 and Cα (Cα had some negative charge characteristics in the intermediate) (Koutmos et al., 2010). A Gly307 residue in human cystathionine β-synthase was predicted to be important for forming the catalytically active conformation state(s) of Tyr308 in the intermediate generation.
It was also reported that cystathionine β-synthase activity might be regulated through different mechanisms. Koutmos et al. analyzed the structure, especially the regulatory and catalysis modules, of Drosophila melanogaster cystathionine βsynthase with two intermediates captured and suggested one allosteric activation mechanism for the enzyme (Koutmos et al., 2010). Based on the structure of the activated enzyme, the authors proposed that S-adenosylmethionine binding activated the enzyme by restricting the conformational flexibility of the regulatory domain to enhance the active site access and stabilize the PLP binding site by introducing changes into a linker region involved in PLP binding (Koutmos et al., 2010). In addition to the allosteric mechanism, a study on human cystathionine β-synthase indicated another activity regulation mechanism, which was through the reducing status of the disulfide bond in the 272 CXXC 275 motif. This motif was in the central domain of human cystathionine β-synthase with either oxidized or reduced states, and the activity of reduced state was higher (2-3-fold) (Niu et al., 2018).
O 2 -Using PLP-Dependent Enzymes PLP-dependent oxidative reactions are not common, although some PLP-dependent enzymes have been reported to catalyze O 2 -involved oxidation side reactions, e.g., dopa decarboxylase. Our recent study indicated that a number of insect PLPcontaining proteins, listed as aromatic amino acid decarboxylases or aromatic amino acid decarboxylase-like enzymes in databases, actually catalyzed the decarboxylation-oxidative deamination reaction of aromatic amino acids to their aromatic acetaldehydes (Vavricka et al., 2011;Liang et al., 2017). Similar proteins were also detected in plants (Kaminaga et al., 2006;Torrens-Spence et al., 2012, 2014. These proteins share high sequence similarity or identity (∼50%) to their respective insect and plant aromatic amino acid decarboxylases, which explains why these proteins have been classified as aromatic amino acid decarboxylases. Our previous studies indicated that these enzymes catalyzed decarboxylation-oxidative deamination to produce the corresponding aromatic acetaldehyde, CO 2 , H 2 O 2 , and NH 3 (Vavricka et al., 2011;Torrens-Spence et al., 2013, 2014Liang et al., 2017). Based on a key role of the aromatic acetaldehyde in insect cuticle formation, the enzyme was arbitrarily named aromatic acetaldehyde synthase, but the mechanism or pathway from aromatic amino acids to their corresponding aromatic acetaldehyde derivatives is unclear (Vavricka et al., 2011;Liang et al., 2017).
Comparative studies on primary structures between identified insect aromatic amino acid decarboxylases and aromatic acetaldehyde synthases showed no apparent clues to tell them apart, but a comparison of 3-dimensional structures or model structures, particularly their active site residues, identified some differences in residues between two enzymes (Liang et al., 2017). In aromatic amino acid decarboxylases, a histidine residue was determined to play an essential role in protonation of carbanionic intermediate, which was considered as a necessary step toward final product formation (Liang et al., 2017). In insect aromatic acetaldehyde synthases, an asparagine residue occupies the similar position (Figure 7). Although aromatic acetaldehyde synthase is also able to catalyze decarboxylation, the rapid protonation of the intermediate becomes problematic. The quinonoid intermediate, once formed, is likely quite reactive, but the absence of the histidine residue prevents its rapid protonation at Cα, which leaves opportunity for oxygen attack (Figure 8) (Liang et al., 2017). This likely increase the life time of the intermediate and gives opportunity for oxygen attack. Instead of protonation and rapid hydrolysis to form dopamine, the rate of the overall reaction of aromatic acetaldehyde synthase slows down greatly with a V max of 270 nmol min −1 mg −1 , which is in contrast with the aromatic decarboxylase with a V max of 3,357 nmol min −1 mg −1 and with the similar level of K m values on the same substrate (3,4-dihydroxyphenylalanine) (Liang et al., 2017). After oxygen addition, the substrate complex likely is released from the enzyme active site and disintegrates rapidly in a buffer environment (Figure 8).
The proposed mechanism was supported by site-directed mutagenesis, in which a mutation of N192 to H192 in a Drosophila melanogaster 3,4-dihydroxyphenylacetaldehyde (DHPA) synthase led to the mutated enzyme with considerable decarboxylase activity and a mutation of H192 to N192 in Drosophila melanogaster 3,4-dihydroxyphenylalanine decarboxylase (DDC) made the mutated DDC have a DHPA synthase activity comparable to the DHPA synthase wild type in magnitude (Liang et al., 2017). Both N192H DHPA synthase variant and H192N DDC variant have not greatly affected substrate affinity as indicated by K m values. K m of DHPA synthase N192H variant increased from 0.88 to 1.23 mM, and K m of DDC H192N variant decreased from 1.26 to 0.82 mM. The N192H DHPA synthase variant decreased the DHPA synthase activity (from 270 to 163 nmol min −1 mg −1 ), but increased the dopamine formation activity (decarboxylase activity) from a negligible level to one of the two major activities (V max of decarboxylase activity was 78 nmol min −1 mg −1 ). DDC H192N variant decreased decarboxylase activity (V max from 3357 to 285 nmol min −1 mg −1 ) and increased the DHPA synthase activity from barely detectable (no more than 1% of the decarboxylase activity) to comparable to that of wild-type DHPA synthase on magnitude (V max is 163 vs. 270 nmol min −1 mg −1 of wild-type DHPA synthase) (Liang et al., 2017).
In DDC-catalyzed reactions, formation of a small amount of DHPA (∼0.3%) has been reported (Liang et al., 2017) Different from insect aromatic acetaldehyde synthases, plant enzymes have His residue conserved in both decarboxylase and aromatic acetaldehyde synthase, but the plant enzymes have a conserved tyrosine or phenylalanine in a flexible loop region functioning to discriminate typical decarboxylation and phenylacetaldehyde synthesis reaction, respectively. Although tyrosine residue in plant decarboxylases may not be able to directly protonate the intermediate, it can stabilize the proton transfer process from histidine residue to Cα after decarboxylation. In contrast, phenylalanine has no stabilizing effect for proton transfer, which leaves the intermediate being attacked by oxygen (Torrens-Spence et al., 2013). The proposed role of the Tyr/Phe residue was supported by sitedirected mutagenesis study. Both Arabidopsis thaliana aromatic acetaldehyde synthase F338Y variant (K m = 4.6 mM) and Catharanthus roseus tryptophan decarboxylase Y348F (K m = 0.095 mM) had little effect on the K m values compared with those of their wild-type enzymes (K m values were 5.1 and 0.12 mM for A. thaliana aromatic acetaldehyde synthase (accession: NP_849999) and C. roseus tryptophan decarboxylase (accession: P17770), respectively), indicating that both mutations did not apparently change the binding affinity to phenylalanine and tryptophan, respectively (Torrens-Spence et al., 2013). The F338Y variant of A. thaliana aromatic acetaldehyde synthase showed decarboxylation activity, which was around 3-fold higher (V max = 341 nmol min −1 mg −1 ) than the original acetaldehyde synthase activity (V max = 112 nmol min −1 mg −1 ) of its wild-type enzyme (Torrens-Spence et al., 2013). By mutating Tyr348 to Phe in C. roseus tryptophan decarboxylase, the decarboxylase enzyme was converted to an aromatic acetaldehyde synthase. The generated acetaldehyde synthesis activity (V max = 160 nmol min −1 mg −1 ) on the same substrate was around 17-fold lower than the original decarboxylation activity (V max = 2,710 nmol min −1 mg −1 ) of the wild-type enzyme (Torrens-Spence et al., 2013).
Although the presence of phenylacetaldehyde synthases has been clearly established (Kaminaga et al., 2006;Torrens-Spence et al., 2012Liang et al., 2017), a number of critical questions remain to be answered. We discussed the involvement of oxygen in somewhat non-enzymatic manner. Oxygen needs to be activated prior to oxidation-reduction processes. However, PLP does not seem to be able on oxygen activation. We propose that the product complex is released first from the active site and then disintegrates to aromatic acetaldehyde, NH 3 , and H 2 O 2 . The disintegration of the complex likely needs acid-base mediated reactions. We proposed the mechanism of this insect phenylacetaldehyde synthase, but the detailed mechanism, including the product complex disintegration, needs to be elucidated. Compared with the enzyme active site environment, acid-base mediated reactions for product complex disintegration may occur easily in a buffer, but the true mechanism may be different from the proposed mechanism. The research on a phenylacetaldehyde synthase from Petunia hybrida cv. Mitchell plant suggests a radical mechanism. The K m of Petunia hybrida cv. Mitchell phenylacetaldehyde synthase is 1.18 mM (Kaminaga et al., 2006), which is similar to the K m of the aromatic acetaldehyde synthase identified from insect (Liang et al., 2017). The k cat is 0.8 s −1 per homotetramer (Kaminaga et al., 2006). The reaction is O 2dependent as confirmed by O 2 consumption. It was suggested that the Petunia hybrida cv. Mitchell phenylacetaldehyde synthase catalyzed oxidative decarboxylation through a radical mechanism (Kaminaga et al., 2006), which was in contrast with the usually proposed non-radical mechanism with a non-radical carbanion or quinonoid intermediate. The authors indicated that the isotope labeling results (about half of the deuterium was found in the phenylacetaldehyde product) better supported the radical mechanism than a non-radical mechanism. These authors argued that if the reaction was through a quinonoid intermediate, all the deuterium should be in the phenylacetaldehyde (Kaminaga et al., 2006). The study on Petunia hybrida cv. Mitchell phenylacetaldehyde synthase provided some clues for a radical mechanism (Kaminaga et al., 2006) and more detailed studies are needed for further clarifications about aromatic acetaldehyde synthase-mediated reactions.
Other oxidative reactions catalyzed by PLP-dependent enzymes were also reported. Han et al. (2015) reported one PLP-dependent L-arginine α-deaminase, γ-hydroxylase, MppP enzyme (accession number: KDR62041, from Streptomyces wadayamensis), could utilize O 2 as a reagent, and the products formed were 2-oxo-5-guanidinovaleric acid and 2-oxo-4hydroxy-5-guanidinovaleric acid. MppP enzyme is the first reported PLP-dependent hydroxylase and quite similar to PLP-dependent aromatic amino acid decarboxylases in the function of stabilizing a quinonoid intermediate for reacting with O 2 . There were two quinonoid intermediates formed in the reaction as supported by the absorbance λ max at 510 nm and 560 nm. The first quinonoid intermediate showed a high stability in the reaction condition without O 2. The K m of MppP enzyme using L-Arg as the substrate was 50.2 µM and k cat was 0.22 s −1 (Han et al., 2015). It was hypothesized that the modest activity level might be a reflection that the full activity of MppP might need other enzyme members in MPP biosynthetic cluster, or that the activity of MppP should be similar to that of nonribosomal peptide synthase (with a slow catalysis) associated with MppP (Sun et al., 2014;Han et al., 2015). Their structural analyses and comparison with PLP-dependent aromatic amino acid decarboxylase suggested that the active site Asp218 residue might contribute to phosphate group rotation. Prior to substrate binding, Asp218 was suggested to interact with PLP phosphate group indirectly. After substrate binding, catalytic Lys211 of the enzyme interacted with Asp218 through a hydrogen bond and Asp218 appeared to make the phosphate group rotate out of the PLP ring plane. Another residue His29 might be the key residue for PLP-dependent hydroxylase (Han et al., 2015). Compared with MppP enzyme, decarboxylases have Asn300 (residue number is from pig kidney decarboxylase, PDB: 1JS3; Burkhard et al., 2001) at the equivalent position as Asp218 in MppP and decarboxylases do not have counterpart residue at the equivalent position with His29. There is one Thr82 residue at the approximate position (Burkhard et al., 2001;Giardina et al., 2011;Han et al., 2015). The authors suggested that analyses of the role of Asp218 and/or His29 were being conducted. His29 was unique to MppP and might be the signature residue of PLP-dependent hydroxylases (Han et al., 2015).
Ringel et al. reported one PLP-dependent periplasmic enzyme PvdN that catalyzed the oxidative decarboxylation using O 2 and glutamic acid as substrates to produce succinamide for the pyoverdine modification (Ringel et al., 2016). This is the first PLP-dependent enzyme that catalyzes the reaction of amide formation after amino acid decarboxylation, O 2 attack and deprotonation. The hypothesized mechanism of PvdN was that a peroxo intermediate formed at Cα after decarboxylation. This step was followed by proton abstraction at Cα, electron shuffling and product complex disintegration to form corresponding amide and H 2 O. The PvdN crystal structure showed a big channel for the pyoverdine side chain to enter from surface to the active site, and the other small channel for O 2 and CO 2 (Ringel et al., 2016). The proposed mechanism of PvdN shares some steps similar to the aromatic acetaldehyde synthase, i.e., decarboxylation and peroxo intermediate formation at Cα, but the key residues that are involved in the amide formation are unknown and need further analyses.
Cap15, a PLP-dependent monooxygenase-decarboxylase, uses O 2 and (5 ′ S, 6 ′ R)-glycyluridine [(5 ′ S, 6 ′ R)-GlyU] as substrates to produce CO 2 and uridine-5 ′ -carboxamide (CarU) (Huang et al., 2018). Unlike aromatic acetaldehyde synthase to form H 2 O 2 as one product, Cap15 catalyzes the incorporation of one O atom into carboxamide containing CarU. Analyses on Cap15 indicated that PLP was the only cofactor for Cap15 to activate O 2 before decarboxylation. They proposed that the carbanionic intermediate, which was the resonance form of quinonoid intermediate, had a role comparable to that of the reduced FAD for monooxygenase. Due to no detectable CO 2 formation when O 2 was excluded from the reaction, it was predicted that O 2 reduction occured before decarboxylation. After deprotonation, the carbanionic intermediate or quinonoid intermediate was formed, and O 2 got one electron from the carbanionic intermediate to form a GlyU-PLP radical and superoxide. The superoxide was protonated and rebound with the radical to form a hydroperoxide species. Then decarboxylation occurred, and one O atom from O 2 was incorporated into the product complex and the other O atom from O 2 was eliminated into H 2 O. The disintegration of product complex released carboxamide-containing CarU (Huang et al., 2018). The reaction catalyzed by Cap15 is PLP-, O 2 , and phosphate-dependent, but the role of phosphate for the reaction catalyzed by Cap15 is unknown. K m of Cap15 is 560 µM and k cat is 0.93 min −1 . Protein sequence alignment indicated that Lys230 was a possible residue that formed an internal aldimine with PLP. Lys262, Lys265, and Lys303 were also predicted as potential residues that were important for Cap15 enzyme. The site-directed mutagenesis result showed that K230A and K303A mutations retained the activity, but K262A and K265A showed no detectable activity (Huang et al., 2018). It was indicated that some kinetic analyses of these variants were ongoing and more structural information would provide insights into the role of these residues and other residues critical for catalysis (Huang et al., 2018). Their study provided one possibility for O 2 activation and should stimulate further research for exploring and verifying the proposed mechanism.
O 2 -using PLP-dependent enzymes have become one emerging field for PLP-dependent enzyme study and many unknown aspects need to be explored. The detailed mechanism for different O 2 -using PLP-dependent enzymes, especially O 2 activation, requires further elucidation. In addition, the structure-function relationship, e.g., residues involved in substrate specificity and reaction specificity, has not been clearly established and awaits further study after characterization of more reactions catalyzed by O 2 -using PLP-dependent enzymes.

SUMMARY
PLP-containing enzymes are diverse and catalyze numerous reactions. Toward this end, scientists have learned sufficiently the chemical characteristics of this cofactor and reactions PLP potentially being able to mediate. Charge interactions, hydrogen bond formation, etc. play some essential roles in PLP-mediated reactions. In given buffer systems, there are plenty of these interactions, but many reactions, which PLP could catalyze when it is associated with protein component, do not proceed or are too slow to be apparent in any buffer system. When PLP is associated with proteins, many interacting factors, readily available in buffer systems, are eliminated. This likely magnifies tremendously the specific interactions of residues with a particular chemical group of PLP moiety and the substrate, thereby promoting specific reactions. Based on the important roles of these interactions, the structure-functional relationship and the mechanism study of PLP-dependent enzymes have become attractive fields of research in recent years. The research advances in several groups of PLP-dependent enzymes have been summarized in this review, with an emphasis on the residues and structural factors that are critical for catalysis and reaction specificity.
A better understanding of structure-function relationships of PLP-dependent enzymes has been achieved as indicated by this review, but there are still many questions left for being explored, e.g., the detailed O 2 activation and attack mechanism remains unknown (Liang et al., 2017;Huang et al., 2018), whether the role of carbanionic intermediate in O 2 activation (Huang et al., 2018) is a common mechanism shared in other PLP-involved oxidative reactions (Kaminaga et al., 2006;Torrens-Spence et al., 2012, 2014, the detailed steps of the product complex disintegration (Liang et al., 2017), and the functional characterization of some uncharacterized PLPdependent enzymes (Vavricka et al., 2011;Liang et al., 2017).

AUTHOR CONTRIBUTIONS
JL wrote the manuscript. QH, YT, HD, and JYL contributed to the modification of the manuscript.