Differential expression of sirtuin family members in the developing, adult, and aged rat brain

The sirtuins are NAD+-dependent protein deacetylases and/or ADP-ribosyltransferases that play roles in metabolic homeostasis, stress response and potentially aging. This enzyme family resides in different subcellular compartments, and acts on a number of different targets in the nucleus, cytoplasm and in the mitochondria. Despite their recognized ability to regulate metabolic processes, the roles played by specific sirtuins in the brain—the most energy demanding tissue in the body—remains less well investigated and understood. In the present study, we examined the regional mRNA and protein expression patterns of individual sirtuin family members in the developing, adult, and aged rat brain. Our results show that while each sirtuin is expressed in the brain at each of these different stages, they display unique spatial and temporal expression patterns within the brain. Further, for specific members of the family, the protein expression profile did not coincide with their respective mRNA expression profile. Moreover, using primary cultures enriched for neurons and astrocytes respectively, we found that specific sirtuin members display preferential neural lineage expression. Collectively, these results provide the first composite illustration that sirtuin family members display differential expression patterns in the brain, and provide evidence that specific sirtuins could potentially be targeted to achieve cell-type selective effects within the brain.


INTRODUCTION
The family of mammalian sirtuins consists of seven members (SIRTs 1-7), which collectively comprise the class III protein deacetylases (reviewed in Michan and Sinclair, 2007). These sirtuins are descendants from the ancestral yeast gene Sir2, which was originally identified as a silent mating factor (Frye, 2000;Greiss and Gartner, 2009). These mammalian sirtuins function as NAD + -dependent protein deacetylases and/or ADPribosylases that exert their actions largely in distinct subcellular compartments. SIRT1, SIRT6, and SIRT7 are found primarily within the nucleus, where they target proteins in nucleoplasm, along heterochromatin and associated with nucleoli, respectively (Michishita et al., 2005). Although predominantly nuclear, there is also evidence that at least SIRT1 can shuttle to the cytosol under certain conditions (Michishita et al., 2005;Jin et al., 2007). In contrast, SIRT3, SIRT4, and SIRT5 localize primarily within mitochondria (Onyango et al., 2002;Ahuja et al., 2007;Nakagawa et al., 2009), while SIRT2 is predominately found in the cytoplasmic compartment of cells (North et al., 2003). Converging evidence has implicated the function of specific sirtuin family members in different aspects of cellular regulation, including lipid homeostasis and metabolism (reviewed in Houtkooper et al., 2012), oxidative stress management (reviewed in Rajendran et al., 2011), and apoptosis resistance (reviewed in Pantazi et al., 2013). As these sirtuin targets are believed to contribute to aging processes, there is speculation that sirtuin family members may play direct roles in longevity regulation (Guarente, 2013). The catalytic activity of the different sirtuins increases or decreases depending on factors such as the availability of NAD + , levels of cellular stress, glucose availability, oxidative load, and/or the energy production demands within the individual cell (Pillai et al., 2005;Yang et al., 2007;Ying and Xiong, 2010;Braidy et al., 2011;Zerp et al., 2014). Thus, sirtuins appear to serve as metabolic and stress sensors whose activity can regulate the homeostatic balance of cells in response to changing environmental conditions. Despite these data illustrating the prominent role of sirtuins in cellular homeostasis, relatively little information currently exists regarding their expression magnitude and spatial distribution within the central nervous system. Given that the brain utilizes approximately 20% of total body energy expenditure (reviewed in Herculano-Houzel, 2011), an understanding of sirtuin expression in this metabolically active tissue could aid in delineating their role in both normal physiological processes and also potentially in pathophysiological conditions. To begin addressing this issue, Cortical tissue was isolated from fetuses obtained at embryonic day 18 (E18) from timed-pregnant Wistar rats (Charles River Laboratories). The head of each embryo was dissected away from the body, and placed in 20 ml 1× Hank's Balanced Salt Solution (HBSS) (Invitrogen-Life Technologies, Burlington, Ontario, cat # 14175103). Brains were removed and placed in a separate dish containing 20 ml supplemented HBSS. Cortices were dissected from whole brains using micro dissection forceps, and the collected tissue was incubated in 2 ml of 0.05% trypsin (Sigma-Aldrich, St. Louis, MO, cat # 59417C) at 37 • C for 15 min. The tissue was then triturated by glass pipette 10-15 times to disperse the cells in seeding medium (Dulbecco's Modified Eagle Medium (DMEM) /F-12) (Invitrogen, cat # 21041025) containing 10% HS and then centrifuged for 5 min at 21,000 × g to pellet the cells. The cells were re-suspended in Neurobasal medium (Invitrogen, cat # 21103049) containing 2% B-27 supplement (Invitrogen, cat # 17504-044), 1% FBS, 0.5 mM L-glutamine (Invitrogen, cat # 25030-149) and cell counts done using Trypan Blue (Sigma-Aldrich, cat # T8154) as a viability indicator. The cells were then seeded in plating medium at a density of 30,000 cells/well in 96-well plates. After 96 h of isolation, the plating media was exchanged with maintenance medium (Neurobasal medium containing 2% B-27 supplement, 0.5 mM L-Glutamine) containing 4 μM cytosine arabinoside (Sigma-Aldrich, cat # C6645) to inhibit proliferation of any residual non-neuronal cells. The primary cultures were fed with maintenance medium every 3-4 days, and the neurons were harvested for assay at 12-14 days after plating.

PREPARATION OF ASTROCYTE-ENRICHED CULTURES
Astrocyte enriched cultures were generated from cortical tissue isolated from post-natal day 1-2 rat pups. In short, following decapitation, cortical tissue was isolated by dissection, placed in ice-cold HBSS, and the meninges removed by dissection. The tissue was then rinsed in HBSS, placed in a minimal amount of media, and chopped into 1 mm 3 cubes by manual dissection. The brain cubes were then incubated with 0.05% Trypsin for 30 min at 37 • C, and dissociated by triturating 12-15 times with polished Pasteur pipette. The cells were then collected by centrifugation at 21,000 × g for 5 min, re-suspended in Astrocyte Medium containing 1× N2 Supplement (Invitrogen, cat # 17502048), 2 mM Glutamax, Penicillin/Streptomycin, supplemented with 5 ng/ml EGF (Sigma-Aldrich, cat # SRP3052) as per manufacturer's instruction. Trypan Blue was used to count viable cells and the cell suspension was then plated in at a density of 1,000,000 cells/well in 6-well plates.

QUANTITATIVE REAL TIME PCR
To quantify sirtuin mRNA expression levels, rats were sacrificed by decapitation, and specific brain regions isolated by dissection on an ice-cold glass plate. RNeasy Mini Kit (Qiagen, Valencia, CA, cat # 74104) was used to extract total RNA from the tissue as per manufacturer's guidelines. Purified total RNA (1 μg) was then reverse transcribed into cDNA using the Superscript II First Strand Synthesis System (cat # 18064-014, Invitrogen). Pre-designed Taqman probe and primer mixtures (Applied Biosystems-Life Technologies, Burlington, Ontario) were used to assess mRNA expression: Sirt2 (cat # Rn01457502_m1), Sirt3 (cat # Rn01501412_m1), Sirt4 (cat # Rn01481485_m1), Sirt5 (cat # Rn01450559_m1), Sirt6 (cat # Rn01408249_m1), and Sirt7 (cat # Rn01471420_m1), using hypoxanthine-guanine phosphoribosyltransferase (Hprt1) (cat # Rn_01527831_g1) as a reference control. Because Taqman primers were not available for rat Sirt1, SYBR Green forward and reverse primers were designed that spanned an intron of the rat Sirt1 sequence (forward: 5cagcaacacctcatgattgg-3 , reverse: 5 -tcccacaggaaacagaaacc-3 ) and also for the reference Hprt1 (forward: 5 -gcagactttgctttccttgg-3 , reverse: 5 -cgagaggtccttttcaccag-3 ). We selected Hprt1 as the housekeeping gene for our mRNA quantification studies as it is expressed ubiquitously and stably in various tissues (Fischer et al., 2005;Wei et al., 2013), and it has been used as an internal control for mRNA normalization in previous developmental studies (Franco-Montoya et al., 2011;Murta et al., 2013). All PCR samples were assayed in quadruplicate on a 384-well plate using Taqman master mix reagent kit (Applied Biosystems, cat # 4304437) or SYBR Green (Applied Biosystems, cat # 4368577) using 20 ng of cDNA generated from total isolated RNA as Coomasie staining was used as the normalization control for examination of peripheral tissue expression levels. This latter means of normalization was required, as classic loading controls such as GAPDH have not proven reliable when comparing across different tissues and/or specific cell types from the same animal (Eaton et al., 2013). Quantity One software (BioRad) was used to analyze scanned Autoradiography films (Denville Scientific, cat # E3018). Optical densities of all bands were measured in OD/mm 2 , with film background subtracted to give the final densitometric values.

STATISTICAL ANALYSIS
Statistical analysis was performed using either student's t-test or One-Way ANOVA in GraphPad Prism version 5.00 (GraphPad Software, San Diego CA), with Tukey's post-hoc test for multiple comparisons where appropriate. All results are expressed as mean ± standard error of means (SEM) and p < 0.05 was set as the level of statistical significance.

Sirt1
Sirt1 mRNA was found by quantitative RT-PCR to be one of the least expressed sirtuin family members in brain. Sirt1 mRNA levels were significantly lower than Sirt2, Sirt3, and Sirt5 in the whole brain, and were present at similar levels to Sirt4, Sirt6, and Sirt7 (Figure 1). Within different brain regions, Sirt1 mRNA was found at similarly low levels, with only modest variability seen between different adult brain regions. The highest Sirt1 mRNA levels were in cerebellum, while the lowest were in spinal cord (Figure 2A). At the protein level, siRNA treatment identified one specific SIRT1 product of 110 kDa (Supplemental Figure  1A). SIRT1 protein prevalence paralleled its mRNA pattern, being highest in the cerebellum, intermediate in the cortex, hippocampus, striatum and the olfactory bulb, and lowest in the spinal cord ( Figure 3A). Relative to its expression in peripheral tissues, Sirt1 mRNA levels in liver were similar to whole brain; spleen and kidney expressed higher levels of Sirt1 mRNA than brain, while heart and small intestine expressed lower levels of Sirt1 than brain (Table 1; Supplemental Figure 3A). This pattern was not recapitulated at the protein level, however, as SIRT1 protein expression was significantly higher in the brain than in any of these peripheral tissues (Supplemental Figure 4A). During brain development, Sirt1 mRNA levels decreased progressively relative to the reference gene Hprt1 from the late embryonic stage in cortex, hippocampus, and cerebellum (Table 2; Figures 4A, 6A, 8A). This developmental mRNA expression pattern was not brain-specific, as a similar pattern was also observed in liver (Supplemental Figure 5A; Supplemental Table 12). At the protein level, this general pattern was recapitulated, as SIRT1 protein expression was significantly higher in cortex and hippocampus at E18 and early postnatal stages compared to the adult and 24 month old stages (Table 2; Figures 5A, 7A). A slightly different pattern was observed in cerebellum, as while SIRT1 protein levels were lower in adult than in perinatal stages, the decrease in prevalence did not occur until after postnatal day 21 ( Figure 9A).
In each brain region, SIRT1 protein prevalence at 3 and 24 months were similar, respectively, indicating the absence of an aging-associated decrease in SIRT1 protein prevalence. This progressive decrease in SIRT1 protein during perinatal development appeared to be brain-selective, as SIRT1 protein levels increased in the liver during perinatal development, and then remained at similar levels until the 24 month stage (Supplemental Figure 6A; Supplemental Table 13).
Analysis of Sirt1 expression between astrocytes and neurons revealed that while Sirt1 mRNA was detected in extracts from each of these respective cultures, its mRNA levels were 84.0 ± 0.3% higher in the astrocyte cultures than in the neuronal cultures (p < 0.05; Figure 10A). Consistent with its low expression in the adult brain, Sirt1 mRNA was also relatively low compared to the other sirtuins except Sirt4 in each of these cultured cell types. At the protein level, though, SIRT1 prevalence differed somewhat from its mRNA profile in these cultures. Although SIRT1 protein was also detected in both the neuron and astrocyte-enriched cultures, its overall prevalence was 68.5 ± 18.4% higher in the neuronal cultures than in the astrocytes (p < 0.05; Figure 10B).

Sirt2
Quantitative RT-PCR revealed that Sirt2 is the most abundantly expressed sirtuin in the rat brain at the mRNA level (Figure 1), where its expression was found to be more than 3-fold higher than the reference Hprt1 gene (Supplemental Figure 2B). Within the different adult brain regions, Sirt2 mRNA was highest in spinal cord, brain stem, and striatum, and at similar levels in cortex, hippocampus, cerebellum and olfactory bulb ( Figure 2B). At the protein level, SIRT2 protein expression patterns were similar its mRNA patterns, being most pronounced in the spinal cord and brain stem, but still showing strong immunoreactive prevalence in cortex, hippocampus, striatum and cerebellum ( Figure 3B). Sirt2 mRNA was also present at high levels relative to its family members in peripheral tissues, but only the kidney expressed Sirt2 mRNA at levels equivalent to those in the adult brain ( Table 1; Supplemental Figure 3B). At the protein level, siRNA treatment identified one specific SIRT2 protein product of 37 kDa (Supplemental Figure 1B). SIRT2 protein prevalence in heart, liver, and spleen were similar to its brain levels, while its protein prevalence in small intestine and kidney were at lower levels than in the adult brain (Table 1; Supplemental Figure 4B).
The developmental expression pattern of Sirt2 mRNA relative to the Hprt1 reference gene in the cortex and hippocampus was highest at E18, and then remained fairly consistent across perinatal development and into adulthood ( Table 2; Figures 4B, 6B). A spike of Sirt2 mRNA was observed at PN21, but this increase was transient and not retained in the 3 month old cortex or hippocampus (Figures 4B, 6B). The expression profile of Sirt2 was somewhat different in the cerebellum, where Sirt2 mRNA displayed a transient increase at PN7 and PN21 compared to E18 and PN2, and then decreased to consistent levels seen in both the adult and 24 month old tissues ( Figure 8B). Intriguingly, however, this mRNA profile for Sirt2 was not recapitulated at the protein level, as SIRT2 immunoreactivity was minimal or at background levels in samples prior to the PN21 stage in cortex, hippocampus, and cerebellum. SIRT2 protein then rapidly appeared at PN21 in each of these brain regions, and remained at fairly consistent Frontiers in Aging Neuroscience www.frontiersin.org December 2014 | Volume 6 | Article 333 | 4 FIGURE 2 | Sirtuins display differential gene expression levels within different regions of the adult rat brain. Histograms showing the mean and SEM of the mRNA expression levels for each individual sirtuin in the indicated brain regions as determined by qRT-PCR. The y-axis shows the mRNA expression levels for (G) Sirt7 relative to the Hprt1 reference gene in each of the denoted brain regions. Data shown are based on the linear conversion of delta CT values for each sample (n = 3, error bars denote SEM). Statistical comparisons for the expression levels of each sirtuin in these adult brain regions are presented in Supplemental Table 2. levels until 24 months of age (Table 2; Figures 5B, 7B, 9B). In adult peripheral tissues, Sirt2 mRNA and protein were expressed at varying levels relative to brain, with SIRT2 protein in heart, liver and spleen similar to or slightly above those found in adult brain (Supplemental Figures 3B, 4B). During liver development, Sirt2 mRNA was expressed from PN2-24 months at similar levels, but was modestly higher at the E18 stage (Supplemental Figure 5B; Supplemental Table 12). SIRT2 protein in the developing liver largely paralleled this pattern, but unlike Sirt2 mRNA, SIRT2 protein displayed a significant increase in prevalence at the 3 month stage, before returning to levels similar to perinatal times at 24 months of age (Supplemental Figure 6B; Supplemental Table 13). The analysis of Sirt2 expression in the neuron and astrocyte cultures also yielded intriguing results. Consistent with its expression levels in whole brain, Sirt2 mRNA was the most prominently expressed sirtuin family member in each culture, although the astrocytes displayed approximately 7-fold higher Sirt2 mRNA than neurons ( Figure 10A). This mRNA pattern was not recapitulated at the protein level, however. In fact, while the densitometric mean and SEM for each specific sirtuin normalized to its corresponding GAPDH loading control (n = 4 independent subjects). Statistical comparisons between sirtuin protein expression patterns in these adult brain regions are presented in Supplemental  Table 3.
SIRT2 immunoreactivity was readily detected in extracts from the astrocyte-enriched cultures, its immunoreactivity in extracts from the neuronal cultures was at, or only slightly above, background levels ( Figure 10C). Thus, despite having pronounced mRNA expression in neurons, SIRT2 protein was not readily detected in the same cultured neurons.

Sirt3
At the mRNA level, Sirt3 was the third most expressed member of the sirtuin family in the whole brain (Figure 1), and in each of the different adult brain regions examined except cerebellum, where its expression was modestly lower (Figure 2C). At the protein level, SIRT3 specific products were identified using cortical tissue derived from a SIRT3-null mouse (Lombard et al., 2007), which identified one specific SIRT3 product of 28 kDa (Supplemental Figure 1C). SIRT3 protein levels largely paralleled its mRNA expression patterns in the adult brain, being at similar levels within cortex, hippocampus, striatum, spinal cord and brain stem, and at modestly lower levels in the cerebellum ( Figure 3C). With respect to peripheral tissues, the kidney displayed Sirt3 mRNA levels similar to those of whole brain, while each of the other tissues examined showed lower Sirt3 mRNA levels than brain ( Table 1; Supplemental Figure 3C). At the protein level, though, there was considerable variations in SIRT3 immunoreactivity in these tissues: SIRT3 protein prevalence in heart was higher than in brain, the spleen and small intestine had lower SIRT3 expression than brain, and the kidney and liver had similar SIRT3 levels as brain (Table 1; Supplemental Figure 4C). The mRNA expression pattern of Sirt3 during development was relatively similar in cortex, hippocampus, and cerebellum, with Sirt3 mRNA levels being fairly consistent from E18 until 24 months of age ( SIRT3 protein expression was quite different, however, as a robust increase in SIRT3 protein prevalence was observed in cortex and hippocampus between PN7 and PN21, and this level was maintained in the 3 and 24 month old samples (Table 2; Figures 5C,  7C). In cerebellum, SIRT3 protein prevalence was similar between E18 to 3 months, but then increased significantly at 24 months of age ( Figure 9C). Sirt3 mRNA and protein expression in the liver across development displayed similarities to its brain expression pattern, as Sirt3 mRNA and protein levels increased dramatically between E18 to PN7, then decreased by PN21 to levels that remained similar at 3 and 24 months of age (Supplemental  Figures 5C, 6C; Supplemental Tables 12 and 13). Within the neuronal and astrocytes cultures, while Sirt3 mRNA was present in both cell types, Sirt3 mRNA levels were 33.1 ± 0.3% higher in the neuronal cultures than in the astrocyte cultures (p < 0.05; Figure 10A). This pattern held at the protein level also, as SIRT3 protein was 63.5 ± 12.7% more prevalent in extracts from the neuronal cultures compared the astrocyte cultures ( Figure 10D).

Sirt4
Sirt4 was found by quantitative RT-PCR to be the least abundantly expressed member of the sirtuin family in the adult rat brain (Figure 1). Its mRNA expression levels ranged from 0.5 to 2% of the reference gene Hprt1 throughout the different regions of the brain, except for spinal cord, which had the highest level of Sirt4 expression at 5% of Hprt1 (Figure 2D). This low level of expression was also seen in the peripheral tissues, where only the kidney expressed Sirt4 at higher levels than brain (Table 1; Supplemental Figure 3D). At the protein level, siRNA treatment identified one specific SIRT4 protein product with a mass of 30 kDa (Supplemental Figure 1D). SIRT4 protein expression was detected in each of the brain regions examined at relatively similar levels ( Figure 3D). This general pattern was also seen in the peripheral tissues, where SIRT4 protein prevalence in heart, liver, and kidney were at levels similar to that of brain, while the spleen and small intestine displayed lower levels of SIRT4 protein than adult brain (Table 1; Supplemental Figure 4D).
During development, Sirt4 mRNA levels in each of the brain regions examined were highest at the E18 stage, decreased significantly from this late embryonic level by PN2, and then remained at fairly consistent levels between PN7 and 24 months of age (Table 2; Figures 4D, 6D, 8D). At the protein level, no significant differences in SIRT4 protein prevalence were noted in any of the brain regions examined throughout development, including the E18 time point where higher mRNA levels had been seen ( Table 2; Figures 5D, 7D, 9D). A similar pattern of mostly consistent expression was also observed in the developing liver, as Sirt4 mRNA levels decreased modestly between E18 to and PN2, and then remained fairly stable until 24 months of age (Supplemental Figure 5D; Supplemental Table 12). Similarly, SIRT4 protein levels did not significantly differ in liver from E18 to 24 months of age (Supplemental Figure 6D; Supplemental Table 13).
Consistent with its low levels of expression levels in the brain and peripheral tissues, Sirt4 mRNA was also the least prevalently expressed sirtuins member in both the neuronal and astrocyte enriched cultures, and no significant difference in Sirt4 mRNA prevalence was observed between the neurons and astrocytes (Figure 10A). At the protein level, SIRT4 protein expression was also detected in each cell type, although SIRT4 protein was 41.2 ± 0.2% higher in the neuronal cultures compared to astrocytes (p < 0.05; Figure 10E).

Sirt5
Quantitative RT-PCR revealed that Sirt5 mRNA is expressed at levels similar to Hprt1 in whole brain homogenates, making it the second most prevalently expressed member of the sirtuin family in the adult brain (Figure 1). However, differences in the magnitude   Table 6.
of Sirt5 expression were observed between different regions of the adult brain. Sirt5 mRNA was significantly higher in brain stem, hippocampus, spinal cord, and olfactory bulb than in cortex and cerebellum (Figure 2E). At the protein level, siRNA treatment identified one specific SIRT5 product of 35 kDa (Supplemental Figure 1E). The adult mRNA expression profile was generally recapitulated at the protein level, although SIRT5 protein in cerebellum was higher than in cortex and hippocampus ( Figure 3E).
In peripheral tissues, Sirt5 mRNA expression in kidney was at levels similar to brain, while all of the other peripheral tissues examined expressed Sirt5 mRNA at levels lower than brain (Supplemental Figure 3E). The protein profile of SIRT5 in these peripheral tissues did not parallel its mRNA pattern, however, as SIRT5 protein was notably higher in liver, spleen, and kidney than in whole brain, and present at lower or non-detectable levels in small intestine and heart (Table 1; Supplemental Figure 4E).

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December 2014 | Volume 6 | Article 333 | 9 FIGURE 5 | Sirtuin protein expression patterns during rat cortical development. Representative Western blots showing the prevalence of (A) SIRT1 (B) SIRT2 (C) SIRT3 (D) SIRT4 (E) SIRT5 (F) SIRT6 in the rat cortex at the indicated stages of embryonic and postnatal development (n = 3 independent subjects). The corresponding GAPDH protein level for the same blot is shown, and serves as a protein loading control. The histograms in each panel show the densitometric mean and SEM for each specific sirtuin normalized to its corresponding GAPDH loading control. The level of expression for each Sirtuin in the 3-month old cortex was arbitrarily set to 100%, and different stages expressed as a percentage of this 3-month value. Statistical comparisons for the expression levels of each sirtuin for the developmental times shown are presented in Supplemental Table 7.
During development, Sirt5 mRNA levels decreased modestly in the cortex and hippocampus between E18 and PN7, and then remained at consistent levels until 24 months of age (Table 2; Figures 4E, 6E). In the developing cerebellum, Sirt5 mRNA levels decreased at 3 months of age, and this lower level remained in tissue from 24 month old rats (Figure 8E). At the protein level, SIRT5 immunoreactivity did not significantly differ across development within either the cortex or hippocampus (Figures 5E,  7E), although SIRT5 protein levels in the cerebellum were higher at 3 and 24 months when compared to levels seen at E18 or in the perinatal stages (Table 2; Figure 9E). During liver development, Sirt5 mRNA expression decreased between E18 and PN2, and then remained fairly consistent until 24 months of age (Supplemental Figure 5E; Supplemental Table 12). The pattern of SIRT5 protein differed from this mRNA profile in liver, however, as SIRT5 protein levels were found to increase progressively until PN21, and then remained at similar levels at 3 and 24 months of age (Supplemental Figure 6E; Supplemental Table 13).
Sirt5 mRNA was present at relatively high levels relative to other Sirtuins in both the neuronal and astrocytic cultures, with its mRNA expression being moderately but significantly higher in neurons by 26.4 ± 0.3% than in astrocytes (Figure 10A). At the protein level, SIRT5 prevalence was more skewed in its neuronal preference, being 201.6 ± 60.8% higher in the neuronal cultures than in the astrocyte cultures (p < 0.05; Figure 10F).

Sirt6
Sirt6 mRNA was found at relatively low levels in the brain by quantitative RT-PCR, being expressed at approximately 10% the level of the Hprt1 reference gene (Figure 1). The highest mRNA  Table 8.
expression for Sirt6 was observed in the olfactory bulb, and the lowest expression levels were found in hippocampus and brain stem ( Figure 2F). A similar low level of mRNA expression was also seen in peripheral tissues, with only the kidney displaying higher Sirt6 mRNA levels than brain, and the liver and heart showing lower expression than brain ( Table 1; Supplemental Figure 3F). At the protein level, siRNA treatment revealed two specific SIRT6 protein products with masses of 36 kDa and 39 kDa (Supplemental Figure 1F). SIRT6 protein expression for each of the isoforms was similar within the different adult brain regions examined ( Figure 3F). In adult peripheral tissues, the expression levels of these two SIRT6 protein forms was variable, with the 36 kDa being expressed highest in the spleen, where its prevalence was greater than in brain, whereas the intestine expressed low or non-detectable levels of this 36 kDa form ( Table 1; Supplemental Figure 4F). In contrast, the 39 kDa form was expressed at levels similar to brain in heart and intestine, while liver displayed low to non-detectable levels of this 39 kDa SIRT6 form.
In the developing brain, Sirt6 mRNA levels were highest in the cortex, hippocampus, and cerebellum at the E18 stage, and then decreased to levels at PN7 that were maintained at 24 months (Table 2; Figures 4F, 6F, 8F). Sirt6 mRNA expression The corresponding GAPDH protein level for the same blot is shown, and serves as a protein loading control. The histograms show the densitometric mean and SEM for each specific sirtuin normalized to its corresponding GAPDH loading control, with the 3-month value arbitrarily set as 100%.
Statistical comparisons for the expression levels of each sirtuin for the developmental times shown are presented in Supplemental Table 9.
in the developing liver similarly decreased during early perinatal development from its late embryonic level, and remained at similar levels until 24 months of age (Supplemental Figure 5F; Supplemental Table 12). At the protein level, the different forms of expressed SIRT6 protein displayed region-specific differences in expression. Throughout cortical and hippocampal development, the prevalence of the 36 kDa product decreased from E18 to PN21, and then remained at similar levels in these respective tissues at 3 and 24 months of age. This was not the case for the 39 kDa SIRT6 protein product, though, as its prevalence remained constant throughout cortical and hippocampal development (Table 2; Figures 5F, 7F). In the developing cerebellum, the 36 kDa SIRT6 product was found at similar levels throughout development, while the 39 kDa form increased in prevalence between E18 and PN7, and then remained at fairly consistent levels until 24 months of age (Table 2; Figure 9F). In the developing liver, the 36 kDa SIRT6 protein form was found at consistent levels throughout development, whereas the prevalence of the 39 kDa product was at low or background levels for each of the developmental stages examined (Supplemental Figure  6F; Supplemental Table 13).
In the neuronal and astrocyte-enriched culture extracts, Sirt6 mRNA was detected in both cell lineages, and at similar levels ( Figure 10A). At the protein level, SIRT6 immunoreactivity was also found in both the neuronal and astrocyte-enriched cultures, although both the 36 kDa and the 39 kDa protein products displayed preferential expression in one cell type. The prevalence of the 36 kDa SIRT6 product was 191.6 ± 63.8% higher in the neuronal cultures than in the astrocytes (p < 0.05; Figure 10G), while the prevalence of the 39 kDa SIRT6 protein form was 32.8 ± 9.5% higher in the astrocyte cultures compared to the neurons (p < 0.05; Figure 10G).

Sirt7
Quantitative RT-PCR revealed that Sirt7 is also expressed at low levels in the adult brain relative to other sirtuin family members, being present at roughly 5% the levels of the Hprt1 reference gene (Figure 1). Within the different regions of the adult brain examined, no significant differences in Sirt7 mRNA expression was detected ( Figure 2G). Interestingly, and in contrast to the other sirtuin family members, Sirt7 mRNA expression was highest in the small intestine (Table 1; Supplemental Figure 3G), where its expression levels were about 10-fold higher than its mRNA levels in brain. Although lower than in small intestine, both the spleen and kidney also showed significantly higher Sirt7 mRNA expression levels than the brain. During cortical and hippocampal development, Sirt7 mRNA levels decreased between E18 and PN2, but then remained at similar levels throughout the remaining time points examined (Table 2; Figures 4G, 6G). In the cerebellum, Sirt7 mRNA The corresponding GAPDH protein level for the same blot is shown, and serves as a protein loading control. The histograms show the densitometric mean and SEM for each specific sirtuin normalized to its corresponding GAPDH control, with the 3-month value arbitrarily set as 100%. Statistical comparisons for the expression levels of each sirtuin for the developmental times shown are presented in Supplemental Table 11. levels remained at relatively similar levels throughout perinatal development, as well as in the adult and 24 month old brain (Table 2; Figure 8G). Across liver development, Sirt7 mRNA levels decreased modestly from E18 to PN21, and then remained at similar levels at 3 and 24 months (Supplemental Figure 5G; Supplemental Table 12). In the neuronal and astrocyte-enriched culture extracts, Sirt7 mRNA was detected in both lineages, with its expression in astrocytes being 60.1 ± 0.1% higher than in neuronal cultures than in astrocytes (p < 0.05; Figure 10A). SIRT7 protein expression patterns could not be determined in this study, as we failed to identify a SIRT7 antibody that yielded a product that could be suppressed by Sirt7 targeted siRNA treatment (see Materials and Methods and Supplemental Figure 1G).

DISCUSSION
In this study we assessed the expression patterns of seven sirtuin family members in specific regions of the developing, adult, and aged rat brain ( Table 2). Four principle findings emerge from our study. First, although each of the sirtuin family members is expressed within the adult and developing rat brain, there is considerable variability in the magnitude of mRNA expression between each of the individual sirtuins, with Sirt2 being the most prominently expressed and Sirt4 being the least expressed. Second, although there is some overlap between specific members, individual sirtuin family members display a unique pattern of temporal and spatial expression within the adult and developing brain. Third, specific members of the sirtuin family display preferential or even selective cell type expression patterns between (F) SIRT5 (G) SIRT6 in cultured astrocytes and neurons. The histograms show the mean and SEM of the densitometric band intensity for each sirtuin normalized to its corresponding β-actin levels. Asterisks denote significant differences in expression levels for the indicated sirtuin between the neuronal and astrocyte cultures (n = 3, p < 0.05, student's t-test).
neurons and astrocytes at the mRNA and protein levels. Fourth, there is notable discordance between the mRNA and protein expression patterns for Sirt2, indicating that its protein expression pattern is likely regulated by post-transcriptional mechanisms. Collectively, these results reveal a contrasting expression pattern for the seven sirtuin family members in the developing and adult brain, and suggest that metabolic processes and/or oxidative defense systems within different regions of the brain, and potentially within specific neural cell types, may be differentially regulated by specific combinations of sirtuins. Although previous studies have indicated different sirtuins are expressed within the brain (Ford et al., 2006;Mostoslavsky et al., 2006;Li et al., 2007;Lombard et al., 2007;Ramadori et al., 2008;Nakagawa et al., 2009), our results indicate that there is considerable divergence amongst the family members in their spatial expression patterns, in their developmental profile of expression, and in the magnitude of their mRNA abundance within specific brain regions. Although none of the adult brain regions we examined was devoid of expressing any sirtuin member, the three most prevalently expressed sirtuins within each brain region were Sirt2, Sirt3, and Sirt5. This general pattern was also recapitulated in the peripheral tissues we examined, although in some peripheral tissues Sirt7 mRNA was more prevalent that Sirt5 or Sirt3. In fact, Sirt7 mRNA levels were significantly higher in peripheral tissues than Sirt7 mRNA levels in any region of the adult brain we tested. This differential Sirt7 mRNA expression pattern may indicate a more prominent role for Sirt7 in tissues with high cell proliferation rates as suggested previously (Ford et al., 2006), as compared to its role within the largely post-mitotic brain.
During development, Sirt1, Sirt6, and Sirt7 mRNA levels were significantly higher in the late embryonic and early postnatal regions of the brain than in the corresponding adult brain regions. Only Sirt4 displayed lower mRNA levels than Sirt1, Sirt6, and Sirt7 in these brain regions during early perinatal development or in the adult stage. The relatively low mRNA levels observed for Sirt1, as well as for Sirt6 and Sirt7, in brain regions from 3 and 24 month old rats relative to their expression levels in the late embryonic and early postnatal times were somewhat surprising, as Sirt1 has been strongly implicated as a neuroprotective factor in models of Huntington's disease (Jeong et al., 2011), Multiple sclerosis (Shindler et al., 2007;Khan et al., 2014), stroke (Yan et al., 2013) and Alzheimer's disease (Qin et al., 2006). However, low basal expression levels could potentially be advantageous for these sirtuins in the brain, as SIRT1 levels have been shown to increase significantly in different cultured cell lines when they are exposed to conditions of energy/nutrient stress (Kanfi et al., 2008;Chen et al., 2011;Li et al., 2013). Similarly, SIRT6 and SIRT7 have been implicated in the maintenance of DNA integrity, and in DNA repair following damage (Ford et al., 2006;Cardus et al., 2013), which is often a consequence of increased cellular stress. Therefore, the relatively lower levels of Sirt1, Sirt6, and Sirt7 present in the adult brain under normal conditions may allow for their pronounced induction under pathophysiological conditions to facilitate a beneficial compensatory response in times of need. It is worth noting that neither the mRNA levels or protein prevalence of either SIRT1 or SIRT6 in the 24 month old cortex or hippocampus differed from their respective levels at 3 months of age, suggesting that aging alone does not alter the expression of these sirtuins. While this suggests the lack of an endogenous age-related compensatory response to aging, these results do confirm the expression of each sirtuin in the aged brain, which therefore validates their pharmacological targeting for age-related conditions. Indeed, brain-specific SIRT1 over-expressing transgenic mice show significant life span extensions (Satoh et al., 2013), suggesting enhancing SIRT1 activity pharmacologically in the brain may yield a similar effect. Likewise, the global over-expression of SIRT6 in transgenic mice similarly extended life span (Kanfi et al., 2012), suggesting SIRT6 may also be a rationale target for drug development. In our study, analysis of SIRT6 protein expression revealed two products at 36 kDa and 39 kDa, which comports with a recent study examining how preterm labor affects SIRT6 expression that also reported the presence of two distinct SIRT6 products (Lim et al., 2013). Interestingly, we show that these distinct SIRT6 forms displayed somewhat opposing expression patterns during brain development: while the 36 kDa product decreased during perinatal development, the 39 kDa product increased as the animal aged. While the reason for the divergent expression pattern remains unknown, these results indicate that an examination of the role played by each in temporal brain development and aging is warranted.
Analysis of the astrocytic and neuronal expression patterns of the predominantly nuclear residing Sirt1, Sirt6, and Sirt7 sirtuins revealed that while each sirtuin is expressed in these two neural cell lineages, their relative expression levels are not equivalent between the two cell types. At the mRNA level, Sirt1, Sirt6, and Sirt7 displayed modestly (approximately 2-fold) higher expression in astrocytes than in neurons relative to the reference gene Hprt1. At the protein level, this pattern of more predominant astrocyte expression was not recapitulated by SIRT1, whose protein prevalence was found to be significantly higher in neurons than in astrocytes. This differential expression profile of Sirt1 mRNA and protein between cultured astrocytes and neurons suggests that post-transcriptional factors may play a role in establishing SIRT1 protein expression patterns in these specific cell types. Importantly, these results also indicate that glial cells should be considered as potential mediators of neuroprotective actions associated with SIRT1-activating drugs (Shindler et al., 2007;Khan et al., 2014). Somewhat surprisingly, SIRT6 protein expression was found to display isoform-specific preferences between neurons and astrocytes. This could indicate the two forms influence specific cell types in the brain in different or specific manners. Favero et al. (2014) recently reported SIRT6 immunoreactivity within the nucleus of cortical neurons in mouse brain. Although this study did not resolve specific forms of SIRT6, or explicitly examine SIRT6 expression in astrocytes, the presence of SIRT6 immunoreactivity in neurons is consistent with our results. In this regard, it is of interest to note that the selective ablation of SIRT6 from neurons in mice results in an adult-onset obesity (Schwer et al., 2010), while liver-specific deletion of Sirt6 in mice results in a "fatty liver" phenotype (Kim et al., 2010). Our results indicate that the 36 kDa product is the form most predominately expressed in neurons, and is the only form reliably detected in the developing and adult liver. By extrapolation, this would suggest the loss of the 36 kDa form may be predominantly responsible for the observed phenotypes in these respective studies. At the least, our results confirm that both SIRT1 and SIRT6 proteins are expressed in varying levels in both neurons and astrocytes which therefore indicates drugs designed to enhance the catalytic activity of either SIRT1 or SIRT6 should elicit effects within both of these cell types-but potentially with differing cell-selective efficacies depending on the sirtuin, or the SIRT6 form, that is being pharmacologically targeted.
In contrast to the relative low expression levels of the nuclear sirtuins, the mRNA expression levels of the cytosolic-nuclear sirtuin Sirt2 were robust. High levels of Sirt2 mRNA were detected ubiquitously throughout the different regions of the adult and developing brain examined, and in each of the peripheral tissues investigated. These results are consistent with a recent study showing Sirt2 mRNA is expressed at high levels in the human cortex (Korner et al., 2013), and illustrate Sirt2 mRNA is expressed at higher levels than any of the other sirtuins in the adult or developing rat brain, peripheral tissues, and also in extracts from both the neuronal and astrocyte-enriched cultures, respectively. However, there is a pronounced disconnect between the high levels of Sirt2 mRNA expression in the embryonic and early postnatal brain, and the low to non-detectable amounts of SIRT2 protein detected by immunoblot in the same tissues. While isoforms of SIRT2 have been reported (Maxwell et al., 2011), the SIRT2 antibody we employed targets a carboxyl region of SIRT2 that is common to the different isoforms that have been characterized to date, suggesting that the differential expression we detect does not arise from the antibody we used missing an isoformspecific product lacking this epitope. This, together with the cell lineage expression results discussed below, raises the possibility that post-transcriptional mechanisms may strongly influence the expression of SIRT2 protein in the developing brain and in specific cell contexts.
There has been controversy to date over which types of neural cells express Sirt2 in the brain. Using antisense mRNA in situ hybridization, as well as anti-SIRT2 immunohistochemisty, Li et al. (2007) reported strong SIRT2 expression in oligodendrocytes, but minimal to non-detectable Sirt2 mRNA expression in neurons or astrocytes. In contrast, Maxwell et al. (2011) reported robust SIRT2 protein expression in neurons, modest expression in oligodendrocytes, and low to undetectable SIRT2 expression in astrocytes through immunohistochemical analysis. Imaoka et al. (2012) reported strong SIRT2 immunohistochemical expression in astrocytes of glioblastoma, astrocytoma, and normal human brain samples. The reasons for the discordance is not clear, but one concern with immunohistochemical studies is whether the employed antibodies label non-specific proteins in addition to their primary intended target that would cloud data interpretation. Our study assessed SIRT2 protein (as well as each of the other Sirtuin family members) by Western blotting rather than immunocytochemistry, which allowed us to focus selectively on an immunoreactive product that could be significantly and selectively suppressed by Sirt2 targeted siRNA treatment (Supplemental Figure 1B). When combined with quantitative PCR outcomes, these immunoblot results reveal an interesting phenomenon: although Sirt2 mRNA is clearly detected at high levels in both neuronal and astrocyte enriched cultures, SIRT2 immunoreactive protein is detected at robust levels only in the astrocyte-enriched culture extracts, with minimal or no protein detected in extracts from the neuronal cultures. While we did not examine oligodendrocytes specifically, our astrocyteenriched cultures displayed only minor immunoreactivity for the oligodendrocyte-specific marker Ng2 relative to whole brain samples, and Ng2 was not detected in the neuronal-enriched cultures (Supplemental Figure 7). This argues against the SIRT2 protein we detect stemming from a contaminating oligodendrocytes presence in the enriched cultures. Furthermore, the developmental expression pattern for SIRT2 in cortex showed similarity to that of the GFAP (Supplemental Figure 8), indicating that the appearance of SIRT2 is largely coincident with a wave of astrogenesis in the developing postnatal cortex. A similar coincident pattern of expression was observed for the 39 kDa form of SIRT6. This coincident expression raises the possibility that the expression patterns for SIRT2 and SIRT6 in the developing brain could relate to increases in astrocyte prevalence. However, given that pronounced Sirt2 mRNA expression was detected in both the neuronal and astrocyte enriched cultures, the relative absence of SIRT2 protein in neurons suggests that SIRT2 protein expression must be negatively regulated in neurons by post-transcriptional mechanisms that either prevent protein from being generated from expressed transcripts, or that rapidly destabilize and degrade SIRT2 protein in cultured neurons upon its synthesis.
The expression patterns of the mitochondrial sirtuins Sirt3, Sirt4, and Sirt5 displayed a wide range of spatial and temporal divergence in the developing and adult brain, as well as considerable differences in magnitude of expression. Sirt5 and Sirt3 mRNA levels were found to be the second and third most abundant sirtuin transcripts in the developing and adult brain, in both the neuronal and astrocyte specific cultures, and in most of the peripheral tissues examined, respectively. The relatively higher expression levels observed for Sirt5 and Sirt3 mRNAs were not unexpected, as these sirtuins regulate mitochondrial homeostasis, metabolism and reactive oxygen species genesis in multiple cell types (Bell et al., 2011;reviewed in He et al., 2012;Webster et al., 2012). We found no evidence for significantly different expression levels of SIRT3, SIRT4, or SIRT5 protein in the brain tissues we assessed from rats at 24 months compared to 3 month of age, indicating an apparent lack of age-associated decrease in the brain expression of these mitochondrial sirtuins. This result contrasts with those of Zeng et al. (2014), who reported decreased SIRT3 protein levels in the aged rat auditory cortex, and Di Loreto et al. (2014) who also reported a decrease in SIRT3 protein levels in the aged mouse cortex relative to young adult under conditions of acute food and water deprivation. The reason for these differing results is unclear, but could relate to cortical subfield specific changes in SIRT3, and/or the influence of different stress conditions presented to the subjects in the studies. Because mitochondrial dysfunction is heavily implicated in aging (reviewed in Lee and Wei, 2012) and SIRT3 modulates mitochondrial metabolism and anti-oxidative defense systems (reviewed in Kincaid and Bossy-Wetzel, 2013), the preserved prevalence of SIRT3, as well as SIRT4 and SIRT5, in the aged brain confirm that drugs selectively targeting any of these sirtuins would be expected to exert effects at later stages of aging. Further, as our results indicate each of these sirtuins are expressed in both neurons and astrocytes, the same drugs would likely affect both neural cell types. Interestingly, although SIRT3 protein has been reported to be expressed two predominant forms: a 45 kDa product corresponding in mass to its precursor form, and a 28 kDa product corresponding to its form following import into mitochondria after processing by mitochondrial processing peptidase (Onyango et al., 2002), we could only verify the 28 kDa product recognized with the antibody we employed as originating from Sirt3 (Supplemental Figure 1C). It is not clear whether a 45 kDa SIRT3 product is also present in brain that is masked in the SIRT3-null mouse brain by a cross-reactive co-migrating non-specific protein. As such, we only report the protein expression profile for the processed form of SIRT3, which could conceivably differ from the 45 kDa form that has been detected in the nucleus (Iwahara et al., 2012). Additional studies will be required to resolve this possibility. Finally, in contrast to the relatively high expression levels observed for Sirt3 and Sirt5, Sirt4 was generally the least expressed member of the sirtuin family in all of the adult tissues and cell types examined. The notably higher level of Sirt4 mRNA expression in late embryonic stage cortex and hippocampus compared to perinatal or adult brain regions suggest Sirt4 may be more prominently expressed during embryogenesis, and that a closer examination of Sirt4 during embryonic brain development may be warranted. In sum, this study illustrates sirtuin family members display differential expression patterns within the developing and adult brain at both the mRNA and protein levels, with specific family members displaying preferential expression in either astrocytes or neurons. Our results indicate that mRNA for each of the seven sirtuins is expressed in the brain throughout development. In all brain regions examined during development, Sirt2 was the most prevalently expressed sirtuin, and Sirt4 was the least expressed member of the family. No significant decreases in the expression of any sirtuin member were observed in any brain region between the 24 month old and 3 month old rats. However, the mRNA expression pattern for specific sirtuins did not always parallel its corresponding protein expression pattern. This was most evident for SIRT2, where protein expression was largely absent at early developmental stages despite the presence of pronounced mRNA expression. This differential expression was also noted in cell type specificity, where Sirt2 mRNA was detected in both astrocytes and neurons whereas SIRT2 protein was selectively observed in astrocytes. Sirt5 also displayed similar differential cell type expression, as SIRT5 protein was preferentially observed in neurons despite its mRNA being present in both astrocytes and neurons. Collectively, these results add to our knowledge of the regulation of sirtuin expression within the brain at different developmental stages, and provide information on which specific sirtuins could potentially be targeted pharmacologically in the brain at times ranging from perinatal development to 24 months of age. These data also provide evidence that specific sirtuins could potentially be targeted to achieve cell-type preferential effects within the brain.