AUTHOR=Genewsky Andreas J., Wotjak Carsten T. TITLE=The Endocannabinoid System Differentially Regulates Escape Behavior in Mice JOURNAL=Frontiers in Behavioral Neuroscience VOLUME=11 YEAR=2017 URL=https://www.frontiersin.org/articles/10.3389/fnbeh.2017.00201 DOI=10.3389/fnbeh.2017.00201 ISSN=1662-5153 ABSTRACT=Among the hardwired behaviors, fear or survival responses certainly belong to the most evolutionary conserved ones. However, higher animals possess the ability to adapt to certain environments (e.g., novel foraging grounds), and, therefore, those responses need to be plastic. Previous studies revealed a cell-type specific role of the endocannabinoid system in novelty fear, conditioned fear and active vs. passive avoidance in a shuttle box paradigm. In this study we aim to investigate, whether knocking-out the cannabinoid receptor type-1 (CB1) on cortical glutamatergic (Glu-CB1−/−) or GABAergic (GABA-CB1−/−) neurons differentially affects the level of behavioral inhibition, which could ultimately lead to differences in escape behavior. In this context, we developed a novel behavioral paradigm, the Moving Wall Box (MWB). Using the MWB task we could show that Glu-CB1−/− mice have higher levels of behavioral inhibition over the course of repeated testing. GABA-CB1−/− mice, in contrast, showed significantly lower levels of behavioral inhibition compared to wild-type controls and more escape behavior. These changes in behavioral inhibition and escape behavior cannot be explained by altered levels of arousal, as repeated startle measurements revealed general habituation irrespective of the line and genotype of the animals. Taken together, we could show that CB1 on cortical glutamatergic terminals is important for the acquisition of active avoidance, as the absence of CB1 on these neurons creates a bias toward inhibitory avoidance. This is the case in situations without punishment such as electric footshocks. On the contrary CB1 receptors on GABAergic neurons mediate the acquisition of passive avoidance, as the absence of CB1 on those neurons establishes a strong bias toward escape behavior.