NALCN (also named Rb21, VGCNL-1, NA in Drosophila melanogaster and NCA-1/2 in Caenorhabditis elegans) was first cloned from rat brain and described by Perez-Reyes and colleagues who named it Rb21 (Lee et al., 1999). With the exception of the nematode Caenorhabditis, the cnidarian Nematostella and the sponge Amphimedon that have two related channels, there is only one gene encoding NALCN in other organisms (Liebeskind et al., 2012; Senatore et al., 2013). In mammals, NALCN is a 1738 amino-acids protein that forms the channel pore of the complex and has a predicted topology similar to voltage-gated sodium and calcium channels (Snutch and Monteil, 2007)(Figure 1A). Unlike other members of the four-domain ion channel family, the S4 transmembrane segments have fewer positive residues, especially in domains 2 and 4, possibly explaining NALCN's voltage insensitivity. The predicted pore region is also unique in that its ionic selectivity motif differs from that of calcium channels (EEEE or EEDD) and sodium channels (DEKA). NALCN's ion selectivity motif (EEKE) is implicated in its specific permeation properties. With the exception of C. elegans and D. melanogaster, alternative splicing events in the pore-forming region of invertebrate NALCN result in a calcium channel-like EEEE motif or a sodium channel-like EEKE (or EKEE) motif that remain to be explored at the functional level (Senatore et al., 2013). These findings suggest that NALCN could behave as a sodium or calcium channel depending on the expressed isoform (Senatore et al., 2013).

In humans, the gene encoding NALCN is located on chromosome 13q33.1 and comprises at least 44 exons (43 coding exons). Several splice variants were identified during our cloning step and by database scanning (unpublished results Figure 1B). Alternative splicing events were found in the intracellular loop linking domains 2 and 3 and in the carboxy-terminus region. Interestingly, there is a long non-coding RNA (lncRNA) gene named NALCN-AS1 partially overlapping with NALCN on the reverse strand (Gene ID: 100885778; http://www.ncbi.nlm.nih.gov/). lncRNAs are thought to function in various cellular contexts, including post-transcriptional regulation, post-translational regulation of protein activity, organization of protein complexes, cell–cell signaling, as well as recombination (reviewed in Geisler and Coller, 2013; Sabin et al., 2013). Importantly, lncRNAs would be associated with a wide range of neurodevelopmental, neurodegenerative and psychiatric diseases, as well as brain cancer (reviewed in Barry, 2014). The specific function of NALCN-AS1 remains to be elucidated.

The first functional characterization of NALCN was described by Ren and colleagues, who identified NALCN as the channel responsible for a tetrodotoxin (TTX)-resistant sodium leak current in mouse hippocampal neurons (Lu et al., 2007). In Nalcn knockout mice, they found that hippocampal neurons were hyperpolarized by ~10 mV compared to wild-type mice (Lu et al., 2007). They concluded that NALCN would contribute to the resting membrane potential in these neurons by eliciting a depolarizing current to counterbalance the hyperpolarizing current induced by two-pore potassium channels (reviewed in Ren, 2011; Lu and Feng, 2012). A similar NALCN-like channel activity was described in neurons from L. stagnalis and C. elegans (Lu and Feng, 2011; Xie et al., 2013). Contrasting with these data, NALCN does not conduct a background current but rather drives an acetylcholine-activated sodium current in the MIN-6 cell line, a pancreatic β-cell model (Swayne et al., 2009). This acetylcholine-activated NALCN current requires the M3 muscarinic receptor (M3R) and occurs though a G protein-independent, Src family of tyrosine kinases (SFK)-dependent pathway. Similarly, NALCN current was found to be activated by substance P (SP) and neurotensin through a SFK-dependent pathway in mouse hippocampal and ventral tegmental area neurons (Lu et al., 2009). It is not clear why NALCN behaves as a leak channel (e.g., looks like spontaneously active) in neuronal cells and not in MIN-6 cells and whether NALCN may be considered as a GPCR-activated channel with a cell type-dependent basal activity or as a constitutively open channel regulated by GPCRs. Although these studies provide the first demonstration for the functional properties and regulation of NALCN channels, much remains to be determined about the functionality of NALCN and the mechanism(s) responsible for its activation. Indeed, it has also been hypothesized that NALCN may not be an ion channel per se but rather an ion sensor (Senatore and Spafford, 2013). Discordance in the field indicates that more work is clearly required to reveal the real “channel” identity of NALCN. In the context of this review, we will consider NALCN as a pore-forming subunit.

Like many ion channels, NALCN is associated with several proteins to form a larger channel complex (Figure 2, Table 1). These interacting proteins are involved in the folding, stabilization, cellular localization, and activation of NALCN.

Wild-type C. elegans travels on a culture plate through the continuous and rhythmic propagation of sinusoidal body bends. A simultaneous loss of both nca-1 and nca-2, or the individual loss of unc-79, unc-80 or nlf-1 results in fainting, a unique motor deficit characterized by periodic halting during movement (Sedensky and Meneely, 1987; Morgan et al., 1988; Rajaram et al., 1999; Humphrey et al., 2007; Jospin et al., 2007; Xie et al., 2013). A dysfunction of premotor interneurons was found to be the primary cause of the failure in the initiation and maintenance of rhythmic locomotion exhibited by fainters (Xie et al., 2013). A “hesitant” walking phenotype (e.g., the walk is interrupted by pauses) is also observed when na or unc-79 are invalidated in D. melanogaster (Krishnan and Nash, 1990; Mir et al., 1997; Guan et al., 2000; Nash et al., 2002; Humphrey et al., 2007). Conversely, gain-of-function mutations in nca-1 lead to exaggerated body bending, termed coiling, in C. elegans (Yeh et al., 2008).

When transitioned between solid and liquid environments, C. elegans switch between two patterns of rhythmic locomotion, crawling and swimming that are distinct in both kinematics and pattern of muscle activity (Pierce-Shimomura et al., 2008). A genetic screen was performed in order to find mutants capable of normal crawling but incapable of normal swimming. unc-79, unc-80 and nca-1;nca-2 mutants were found to be paralyzed upon immersion in liquid. The paralytic defect in swimming does not seem to be explained by general defects in neuronal excitability, synaptic function, or development because the fainting phenotype was not observed in mutants defective in voltage-gated calcium channels or major synaptic proteins.

Several studies reported an altered sensitivity to GAs both in invertebrates and mice mutants with some discrepancies. In C. elegans, nca-1;nca-2, unc-79, and unc-80 mutants are hypersensitive to the immobilizing effect of halothane (~2–3 fold increase compared to controls) and other anesthetic agents (Sedensky and Meneely, 1987; Morgan et al., 1988, 1990; Humphrey et al., 2007). In fact, unc-79 mutants are hypersensitive to thiomethoxuflurane, methoxyflurane, chloroform, and halothane. They are also more resistant to flurothyl and enflurane, insensitive to fluroxene and isoflurane, and mildly more sensitive to diethylether (Morgan and Cascorbi, 1985; Morgan et al., 1988, 1990; Humphrey et al., 2007). By contrast, hypomorphic na^har38 and na^har85 drosophila mutants were first described as resistant to halothane, methoxyflurane, chloroform and trichloroethylene but not to diethylether, isoflurane, and enflurane (Krishnan and Nash, 1990; Nash et al., 1991; Campbell and Nash, 1994; Mir et al., 1997). However, it was then reported that unc-79 and na mutants are hypersensitive to halothane but not enflurane (Guan et al., 2000; Humphrey et al., 2007). The fact that na mutants were first described as resistant instead of hypersensitive may be explained by the method used to score anesthetic sensitivity but in some cases also by the fact that different alleles were tested. In another study, na^har38 mutation was found to be hypersensitive to isoflurane and to increase the potency with which isoflurane alters local field potentials recorded directly from fly brains (Van Swinderen, 2006). It was also shown that halothane presynaptically depresses synaptic transmission in wild-type drosophila larvae but not in na^har38 and na^har85 mutants (Nishikawa and Kidokoro, 1999). In mammals, heterozygous Lightweight mice, that carry a hypomorphic mutation in Unc-79, exhibit no alteration in Minimum Alveolar Concentration (MAC) in response to halothane, cyclopropane, or sevoflurane. However, a significant resistance to isoflurane-induced anesthesia was described (Speca et al., 2010).

It remains to be determined if the NALCN channel complex is a direct target for GAs, is important for the function of cells that contain such targets, or influences anesthesia more indirectly. GAs produce a widespread neurodepression in the CNS by enhancing inhibitory neurotransmission and reducing excitatory neurotransmission. However, the action mechanisms of GAs are not completely understood. Several ion channels and GPCRs are affected by these compounds (reviewed in Chau, 2010; Minami and Uezono, 2013). Of interest, it is known for a long time that GAs such as halothane, hyperpolarize neurons by acting on the potassium currents likely by activating two-pore potassium channels (Patel et al., 1999; Gruss et al., 2004; Liu et al., 2004). Considering that neurons from Nalcn knockout animals exhibit a ~10 mV hyperpolarization of their resting membrane potential, it is tempting to hypothesize that, in the case of an observed hypersensitivity, the absence of NALCN could remove a kind of brake for some GAs to act on their targets such as two-pore potassium channels. In this scheme, it would explain why, contrasting with C. elegans and D. melanogaster unc-79 and unc-80 mutants, heterozygous lightweight mice do not display any hypersensitivity to halothane. In fact, NALCN current and protein were found to be still present in Unc-79 deficient mice whereas this is not the case in C. elegans and D. melanogaster (Yeh et al., 2008; Lu et al., 2010; Speca et al., 2010; Lear et al., 2013). An observed resistance to other GAs implies that the NALCN channel complex is a direct target while an absence of phenotype to other GAs would indicate that other targets beside NALCN are involved.

In addition to GAs, alteration in ethanol sensitivity was described in animal mutants for the NALCN channelosome. Morgan and Sedensky, in 1995, found mutations in several genes in C. elegans which seem to control the sensitivity to ethanol, including unc-79 (Morgan and Sedensky, 1995). As a matter of fact, unc-79 mutants showed a decrease by about 25% in ethanol sensitivity. By contrast, it was recently reported that unc-79, unc-80, and nca-1;nca-2 mutants show hypersensitivity to ethanol (Speca et al., 2010). This hypersensitivity seems to be conserved in mammals as heterozygous Lightweight mice exhibit a highly significant increase in the sensitivity to the acute sedative effects of ethanol (Speca et al., 2010). Heterozygous Lightweight mice also present an increased ethanol preference and consumption, compared to wild-type mice, particularly at higher alcohol concentrations (Speca et al., 2010). As with GAs, the nature of the interaction between ethanol and NALCN physiology remains to be studied.

In 2007, it was shown that the Nalcn gene is crucial for survival in mammals (Lu et al., 2007). Homozygous Nalcn knockout mice pups appear normal up to 12 h after birth and then die within 12 h due to severely disrupted respiratory rhythms (Lu et al., 2007). While wild-type mice had no abnormalities, knockout animals' respiration was highly sporadic. Breathing was characterized by ~5 s of apnea, followed by a burst of deep breathing for ~5 s, and this occurred at a rate of ~5 apnea events/minute. Interestingly, this pattern is reminiscent of the periodic breathing of Cheyne-Stokes respiration found in humans with CNS damage (reviewed in Strohl, 2003). Electrophysiological recording from the fourth cervical nerve root that innervates the diaphragm revealed that rhythmic electrical activity present in wild-type mice was largely absent. Thus, the defects observed in the respiratory rhythm in knockout mice are likely to reflect defects in electrical signaling in the nervous system (Lu et al., 2007).

More recently, Lu and Feng, 2011, investigated the properties of a NALCN ortholog in the snail L. stagnalis and its role in the activity of a respiratory pacemaker neuron (Lu and Feng, 2011). An in vivo investigation of its role on regulating respiratory behavior was performed by using RNA interference approaches. Animals in which nalcn was knocked down showed a reduced total breathing time compared to the naïve control. The resting membrane potential of the right pedal dorsal 1 (RPeD1) neuron that initiates the respiratory rhythm was found to be hyperpolarized by ~15 mV and its rhythmic firing was abolished. Thus, NALCN also plays a role in maintaining the respiratory activity in adult animal. It remains to be demonstrated if NALCN plays the same role in mammals by studying its functional properties in the preBötzinger complex and the retrotrapezoidnucleus/parafacial respiratory group that are involved in the respiratory rhythmogenesis (reviewed in Feldman et al., 2013).

Several studies performed with D. melanogaster mutants revealed the NALCN channelosome as an important player of circadian rhythms. Indeed, null na mutants display disrupted circadian rhythm. Typically, wild-type D. melanogaster are diurnal, with a greater proportion of their activity occurring during the daytime but null na mutants exhibit most of their activity at night (Nash et al., 2002). Furthermore, light enhances the climbing deficit that is induced by GAs in these flies (Campbell and Nash, 2001). Interestingly, the amount of NA protein does not fluctuate over time or light cycles indicating that expression of NA is not under circadian regulation. The phenotype seems to be the result of a broad aberrant motor response to photic input. Central to this, na is expressed in circadian pacemaker neurons and regulates the output of these neurons (Lear et al., 2005). An accumulation of Pigment-Dispersing factor (PDF), a neuropeptide essential for robust circadian behavior, is observed in these neurons from na mutants. This suggests that na may be required for PDF release. It is not surprising to observe a disturbance of circadian rhythms in na null mutants as electrical activity is an important regulator of clock function both in invertebrates and mammals (Nitabach et al., 2002; Belle et al., 2009). Null mutations of unc-79 and unc-80 in D. melanogaster also display severe defects in circadian locomotor rhythmicity that are indistinguishable from na mutant phenotypes, and support the role of this channelosome in circadian rhythms (Lear et al., 2013). Knockdown of nlf-1 in D. melanogaster was also found to phenocopy na knockdown for the circadian locomotion phenotype (Ghezzi et al., 2014).

Involvement of the NALCN channelosome in social clustering, the natural tendency of animals of the same species to congregate in close proximity within a group, was recently described. Indeed, in a study aiming to investigate resource-independent local enhancement (RILE) in D. melanogaster, Burg et al., 2013, reported that na^har38 mutants display a deficient social phenotype compared to wild-type flies (Burg et al., 2013). This deficiency is rescued by the expression of na under the control of its native promoter. In addition, a complete rescue of the phenotype is also observed when na is expressed in cholinergic neurons and a partial rescue is obtained when na is expressed in glutamatergic neurons. na is strongly expressed in the mushroom body, a structure known to play a role in olfactory and visual learning and memory and the specific blockade of na expression in this structure affects RILE behavior. Knockdown of nlf-1 in D. melanogaster was also found to significantly suppress the social clustering phenotype as for na (Ghezzi et al., 2014).

The na mutant flies are noticeably smaller than controls and their abdomens are more slender and elongated but no obvious deformity has been identified (Krishnan and Nash, 1990; Mir et al., 1997; Nash et al., 2002). D. melanogaster bearing mutations in unc-79 also exhibit a cylindric shaped abdomen (Humphrey et al., 2007). It is not known if this alteration represents a developmental defect or reflects altered physiology. However, Nalcn knockout mice neonates do not display gross abnormalities in embryonic development, righting responses, spontaneous limb movement, and toe/tail pinch responses (Lu et al., 2007).

Heterozygous Lightweight mice are smaller (shorter in length and lower in body weight) and have a leaner body composition (increased lean tissue and decreased body fat) than wild-type mice (Speca et al., 2010). Interestingly, heterozygous Lightweight mice display an increased food intake in comparison with wild-type mice of the same weight.

A genetic analysis performed in mice demonstrated that Nalcn is involved in systemic osmoregulation by controlling the serum sodium concentration (Sinke et al., 2011). Furthermore, this study reported that heterozygous Nalcn knockout mice exhibit a significant hypernatremia.

With others channels, such as transient receptor potential canonical (TRPC) channels, NALCN is partly responsible for the SP-induced depolarization and regulation of the intestinal pacemaking activity in the interstitial cells of Cajal (Kim et al., 2012). This activity generates the phasic contractions of the gastrointestinal muscles. Of note, in this study, the authors found that NALCN is not required for the basal pacemaking activity in ICCs.

Infantile neuroaxonal dystrophy (INAD) is a rare neurodegenerative disease characterized by progressive motor, mental and visual deterioration that begins in infancy. Onset is usually between the age of 6 months and 3 years and death typically ensues before the age of 10 years (reviewed in Gregory et al., 1993). Early clinical manifestations include bilateral pyramidal tract signs, truncal hypotonia, cognitive decline and optic atrophy. Most cases present with distal axonal swelling and spheroid bodies in tissue biopsies (Cowen and Olmstead, 1963; Morgan et al., 2006). Originally, this recessive disorder was linked solely to mutations in the PLA2G6 gene, which encodes the calcium-independent phospholipase A2β (iPLA2β) enzyme, also designated iPLA2-VIA (Khateeb et al., 2006; Morgan et al., 2006). Recently, a study reported a mutation in the NALCN gene in two affected siblings with additional atypical features including facial dysmorphism, pectus carinatum, scoliosis, pes varus, zygodactyly and bilateral cryptorchidism. The patients also exhibit cerebellar atrophy and seizures and are still alive at 18 years-old (Koroglu et al., 2013; also see Seven et al., 2002). The identified mutation was a C to T conversion in NALCN coding exon 16 (c.1924C > T), creating a premature translational termination signal at codon 642 (Q642X) and truncated NALCN channel (Figure 1B). Interestingly, our unpublished data indicate that this exon is alternatively spliced resulting in the loss of one isoform but not NALCN in its entirety and may explain why the mutation is not lethal. It remains to be established whether this mutation results in a mRNA decay or expression of a truncated two-domain isoform. The fact that PL2G6 and NALCN genes mutations result in a similar disease raises the possibility that iPLA2β could be a regulator of NALCN.

Mutations in the NALCN gene were recently reported in six patients with an autosomal-recessive syndrome characterized by severe hypotonia, speech impairment, and cognitive delay from two large consanguineous families (Al-Sayed et al., 2013). Three male patients (ranging from 4 to 7 years-old) from one family are homozygous for a single nucleotide deletion c.1489delT located in the coding region for the S4 helix from domain II (Figure 1B). This mutation results in a frame shift creating a stop codon 21 amino-acids downstream, which likely results in the production of a truncated protein or mRNA decay. Unless the involved exon is alternatively spliced, it is not clear why this deletion is not lethal as reported for Nalcn knockout mice (Lu et al., 2007). These patients have an absence of speech development, severe hypotonia, chronic constipation, global developmental delay, and facial dysmorphism which is reminiscent of infantile hypotonia with psychomotor retardation and characteristic facies (IHPRF, OMIM #615419). Three female patients (ranging from 9 to 17 years-old) from a second family are homozygous for a missense mutation c.3860G > T (W1287L) in exon 34 that encodes for the S4 helix from domain IV (Figure 1B). These patients have a milder but similar phenotype as the first family with additional features such as seizure disorder and hyperactivity.

Dystonia is a “syndrome of sustained muscle contractions, frequently causing twisting and repetitive movements or abnormal postures” (reviewed in Fahn et al., 1998). Estimates of the prevalence of dystonia range from ~1:10,000 to more than 1:200 (Nutt et al., 1988; Nakashima et al., 1995; Epidemiological Study of Dystonia in Europe Collaborative, G., 2000; Muller et al., 2002). Clinical presentation of dystonia is heterogeneous, from focal involvement such as cervical dystonia to generalized torsional dystonia. Cervical dystonia is the most common form of dystonia (Butler et al., 2004; Jankovic et al., 2007; Groen et al., 2013). The majority of cervical dystonia is transmitted in a non-Mendelian pattern, which suggests cervical dystonia is likely a complex disease rather than monogenic form. A recent genome-wide association study (GWAS) in 212 patients with this form of dystonia revealed a possible association with NALCN (Mok et al., 2013). However, this study did not find any single nucleotide polymorphisms (SNPs) with genome-wide significance (defined as P < 5 × 10⁻⁸) but a few clusters of possible association (defined as P < 5 × 10⁻⁶). Six SNPs within or in the vicinity of the NALCN gene with P-values ranging from 2.54 × 10⁻⁶ to 9.76 × 10⁻⁷ were found. Additional GWAS with larger cohorts of patients are required since the small sample size for this initial study was underpowered to detect loci with small disease effects.

Epilepsy, a very common neurological disorder, is defined by the occurrence of unprovoked seizures caused by the synchronous discharge of large number of neurons (reviewed in Sander, 2003; Khan and Al Baradie, 2012). Abnormal expression or function of ion channels may be involved in the pathophysiology of both acquired and inherited epilepsy (reviewed in Mantegazza et al., 2010; Lerche et al., 2013). Considering the importance of the NALCN channel complex in setting the resting membrane potential of neurons, the NALCN channelosome gene mutations are clear candidates for epilepsy. As mentioned in this review, patients with INAD or an autosomal-recessive syndrome with severe hypotonia, speech impairment, and cognitive delay have seizures (Al-Sayed et al., 2013; Koroglu et al., 2013). Seizures were also reported for patients with chromosome 13q deletions in regions that contain the NALCN gene (Kirchhoff et al., 2009; Lalani et al., 2013). Recently, a whole genome linkage analysis in three-generations of a south Indian family who had multiple members affected with juvenile myoclonic epilepsy (JME) found a critical genetic interval of 24 Mb between markers D2S116 and D2S2390 (Ratnapriya et al., 2010). The UNC-80 gene lies in this region. In addition, a region-wide linkage analysis carried out in 118 European families with an aggregation of JME indicated a significant linkage with marker rsD2S143 located on chromosome 2q34 at around 4Mb of the UNC-80 gene (EPICURE Consortium et al., 2012).

Autism Spectrum Disorders (ASDs) are neurodevelopmental disorders characterized by impairments in social interaction and communication, and the presence of restrictive and repetitive behaviors (reviewed in Jones et al., 2014). Exome sequencing of 343 families, each with a single child on the autism spectrum and at least one unaffected sibling, revealed de novo small indels and point substitutions in the affected sibling (Iossifov et al., 2012). One of the discovered mutations resulted in a truncating R518X mutation in one copy of UNC-80, suggesting that UNC-80 is a susceptibility locus in autistic spectrum.

The 13q syndrome is caused by structural and functional monosomy of the 13q chromosomal region and was first described in 1963 and consequently delineated as a specific syndrome in 1969 (Lele et al., 1963; Allderdice et al., 1969). Large numbers of patients with deletions of the long arm of chromosome 13, which includes NALCN and NLF-1, have been described in the literature. The phenotypes of these patients varied widely. Some showed severe malformations involving the brain, heart, kidneys, lungs, other organ systems, and digits while others were only mildly affected with minor dysmorphic features, developmental delay, and growth failure. The characteristic features of 13q deletions include growth retardation with microcephaly, facial dysmorphism, congenital heart, brain, and kidney defects (Brown et al., 1995). Several studies addressed karyotype-phenotype correlations depending on the extent of the deletions (Brown et al., 1995; Luo et al., 2000; McCormack et al., 2002; Alanay et al., 2005; Garcia et al., 2006; Ballarati et al., 2007; Walczak-Sztulpa et al., 2008; Kirchhoff et al., 2009; Quelin et al., 2009). It was reported that the severely malformed phenotype results from the deletion of a critical region lying between markers D13S136 and D13S147 (Brown et al., 1995). This region of around 2.3 Mb contains several genes including NALCN. Recently, a molecular-karyotyping study reported that a female with congenital heart defects, facial anomalies, developmental delay, and intellectual disability had a deletion of 12.75 Mb extending from 13q33.1 to 13q34 (Huang et al., 2012). The deleted region harbors 55 genes, including NALCN and NLF-1. Another study further refined a critical region for all CNS anomalies and neural tube defects taken together, to a region of 1.6 Mb (Kirchhoff et al., 2009). Only seven known genes are located within this region, including NALCN. A recent study aiming to identify rare DNA copy number variants in cardiovascular malformations with extracardiac abnormalities revealed the deletion of 641 kb in the NALCN gene region in 3 patients (Lalani et al., 2013). The extracardiac phenotypes of these patients include agenesis of corpus callosum, growth retardation, facial dysmorphism, and seizures.

Alzheimer's disease (AD) is the most common form of dementia and the most frequent degenerative brain disorder encountered in old age. The risk of developing AD substantially increases after 65 years of age (reviewed in Nussbaum and Ellis, 2003). With the exception of rare cases of early onset familial Alzheimer's disease caused by mutations in the amyloid precursor protein or presenilins genes, the etiology of the vast majority of cases remains misunderstood. There is evidence for substantial genetic influence (reviewed in St. George-Hyslop and Petit, 2005; Tanzi and Bertram, 2005). A linkage analysis to detect novel AD loci from 437 families (1252 individuals) revealed a statistically significant linkage with marker D2S2944 located in 2q34 in 31 American families with a minimum age at onset between 50 and 60 years (Scott et al., 2003). This marker is located at less than 4Mb from the UNC-80 gene. Another study reported the existence of a susceptibility locus in 14q32.12 near marker D14S617 in a Caribbean Hispanic cohort of 1161 individuals from 209 families (Lee et al., 2008). The UNC-79 gene lies in a region at less than 3Mb from this marker. The existence of a susceptibility locus for AD in the vicinity of the UNC-79 gene (SNP rs11622883) was also reported by Grupe et al., 2007, from a cohort of 1808 American-European patients (Grupe et al., 2007).

Alcohol dependence is one of the most common and costly public health problems. Several studies suggest a role of genetic factors and few genes have been shown to be associated with alcohol dependence (reviewed in Morozova et al., 2014). A recent GWAS performed in 118 European-American families (2322 individuals) demonstrated a significant linkage of SNP rs17484734, located in the NALCN gene, and high-risk of alcohol dependence (Wetherill et al., 2014). Interestingly, mice that heterologously carry a hypomorphic mutation in the Unc-79 gene voluntarily consume more ethanol than wild-type littermates (Speca et al., 2010). A GWAS on comorbid alcohol/nicotine dependence in 599 cases and 488 controls found SNP rs12882384 as one of the three top findings, which is located within the UNC-79 gene (Lind et al., 2010). Genome-wide significance was found for joint alcohol/nicotine comorbidity but not for nicotine or alcohol dependence alone suggesting the existence of genes that mediate the combined effect of these two addictive substances. In addition, two previous studies suggest the existence of a susceptibility locus located on chromosome 2, near the UNC-80 gene, linked to alcohol tolerance (markers D2S425, D2S434, D2S424, D2S1323, D2S1333) and the comorbidy of alcoholism and depression (marker DS1371) respectively (Nurnberger et al., 2001; Schuckit et al., 2001).

Restless legs syndrome (RLS) is a sensorimotor disorder characterized by abnormal sensations in the limbs that are both dependent on activity and time of day, such that symptoms are promoted by rest and relieved by activity and peak in the evening or at night (reviewed in Winkelman et al., 2013). RLS is a genetically heterogeneous complex trait with high prevalence but large phenotype variability. Current theories of RLS pathophysiology emphasize brain iron deficiency with abnormal dopaminergic consequences, together with a strong underlying genetic background (reviewed in Dauvilliers and Winkelmann, 2013). A recent study analyzing 11 individuals from the same family indicated the existence of a locus on chromosome 13q, between SNPs rs2182885 and rs7333498 (around 5.3 Mb in size; Balaban et al., 2012). The NALCN gene lies in this region.

Primary biliary cirrhosis is a chronic granulomatous cholangitis, characteristically associated with antimitochondrial antibodies. Analysis of both aggregation data from families and concordance data from twins revealed a genetic predisposition for the disease (reviewed in Hirschfield and Gershwin, 2013). A GWAS with a cohort of 2072 North-American individuals (536 patients and 1536 controls) was carried out by genotyping more than 300,000 SNPs (Hirschfield et al., 2009). Although this study reported the strongest association of the disease with HLA-related genes, a significant association was found with SNP rs2211312 in the NLF-1 gene.

Hypertension is a common human disease affecting over one billion people world-wide and a major contributor to cerebrovascular accidents, myocardial infarction, congestive cardiac failure and chronic renal failure (reviewed in Zhao et al., 2013). A GWAS performed by genotyping more than 800,000 SNPs in a sample of 1017 African-American individuals led to the identification of several potential loci involved in the regulation of blood pressure (Adeyemo et al., 2009). A significant association was found between systolic blood pressure and SNP rs9301196 located within the NLF-1 gene.

Polyglutamine (polyQ)-expansions in different proteins cause at least eleven neurodegenerative diseases including Huntington disease and spinocerebellar ataxias (reviewed in Todd and Lim, 2013). These human diseases are caused by extreme expansion of the repeats, which adversely impact protein structure, often causing intracellular protein aggregation and altered protein function. Bovine NLF-1 exhibits a polymorphic polyQ tract in its sequence (Whan et al., 2010). This polyQ tract is also found in other species including human beings suggesting that aggregation of NLF-1 could be involved in neurodegenerative diseases.

Biankin et al. examined pancreatic cancer genomes using exome sequencing and copy number analysis from a cohort of 142 patients of early (stages I and II) sporadic pancreatic ductal adenocarcinoma (Biankin et al., 2012). Analysis of genes with non-silent mutations occurring in 2 or more individual cancers identified 16 genes including NALCN (mutated in 4 patients). Pathway analysis allowed not only the identification of genes involved in mechanisms already known to be involved in cancer but also genes known to be involved in axon guidance. This latter finding was strengthened by the inclusion of data from another study (Jones et al., 2008). It is tempting to speculate that NALCN could also be involved in axon guidance.

An association of NALCN with non-small cell lung cancer was also suggested by a study where 217,817 SNPs were genotyped in 348 advanced patients who received chemotherapy (Lee et al., 2013). These genetic association studies revealed that SNP rs9557635, located in the genomic regions of the NALCN gene, was associated with this disease. Interestingly, this study also reported a strongly significant association with SNP rs2371030 located 25 kb downstream to the CPS1 gene on chromosome 2q34, which contains the UNC-80 gene. Further linking the channelosome to cancer, a recent study reported an NLF-1 copy number increase in populations of tumor-derived endothelial cells that are resistant to anti-angiogenic cancer therapies (McGuire et al., 2012). Finally, in a search for key altered genomic regions in human glioblastomas, Fontanillo et al., 2012, found a high correlation between the malignant state and copy number alterations in the NALCN and NLF-1 regions with a decrease in expression level (Fontanillo et al., 2012).

From a fundamental point of view, it remains unclear whether NALCN is truly an ion channel. The early findings by Ren and colleagues supported that NALCN was an ion channel, based on mutations in the putative selectivity filter (EEKE) that alter the permeation properties and the gadolinium block (EEKA) or inhibit the observed currents in HEK-293 cells (EEEE)(Lu et al., 2007). Further supporting this, in D. melanogaster, the expression of a na^EEEE mutant in na neurons using the UAS-GAL4 system did not restore the studied phenotype as na^wt does (Lear et al., 2005). However, functional expression of NALCN channel in heterologous expression systems is difficult to obtain and these data do not demonstrate unambiguously that NALCN is a pore forming subunit. A clear proof of its channel activity would come from electrophysiological data exploring NALCN channel activity when rebuilt in lipid bilayers.

The mechanism(s) that gate NALCN have yet to be established. On one hand, NALCN channel is described as conducting a sodium leak current in neurons both in mammals and invertebrates (Lu et al., 2007; Lu and Feng, 2011; Xie et al., 2013). On the other hand, NALCN channel also conducts a sodium current activated by acetylcholine though the M3R in a pancreatic β-cell line without any evidence for a leak current (Swayne et al., 2009). One may hypothesize that the NALCN-mediated leak current observed in neurons may result from a constitutive activity of some GPCRs (Swayne et al., 2009). In this case, it would require UNC-80 and SFKs. However, this hypothesis may not be valid for two main reasons. First, considering that HEK-293 cells do not express UNC-79 and UNC-80 (Swayne et al., 2009), the expression of NALCN alone is sufficient to observe a sodium leak current in these cells (Lu et al., 2007). Second, it was shown that a sodium leak current mediated by NALCN is still present in hippocampal neurons from Unc-79 knockout mice where UNC-80 is not detected (Lu et al., 2010). Thus, the gating properties of NALCN remain mysterious and should be investigated further. In keeping with this idea, the search for additional proteins that belong to the NALCN channelosome could give clues regarding modulation of the NALCN gating mechanism.

Recent studies have established that NALCN can be activated/potentiated or inhibited by different GPCRs (Lu et al., 2009; Swayne et al., 2009; Lu et al., 2010). Therefore, one may expect additional GPCRs to be involved in these processes. Identification of this repertoire is an important challenge and understanding why some GPCRs modulate NALCN, and some others do not, would help to understand further how NALCN is activated/regulated in neurons and how neurotransmitters and hormones affect neuronal electrophysiological properties.

Alternative splicing events were demonstrated in many ion channels and can significantly impact their biophysical properties, cellular localization, and functional regulations (reviewed in Noel et al., 2011; Jan and Jan, 2012; Lipscombe et al., 2013). Alternative splicing was identified in NALCN channelosome genes (Senatore et al., 2013; Monteil unpublished data). The physiological consequences of these alternative splicing events remain to be elucidated.

In C. elegans, nlf-1, unc-79, unc-80, and nca-1;nca-2 mutants exhibit exactly similar phenotypes and both Unc-79 and Nalcn knockout mice die soon after birth. However, the post-natal lethality observed in these mouse models could mask phenotypes more specific to one subunit compared to the others. As a matter of fact, we cannot exclude that UNC-79, UNC-80, and NLF-1 may have NALCN-independent physiological roles. Conditional knockout mice for each component of the NALCN channelosome would be very helpful to clarify this point.

The NALCN channelosome is probably not restricted to NALCN, UNC-79, UNC-80, NLF-1, SFKs, and GPCRs. As an example, there is another gene highly related to NLF-1, FAM155B in mammals (accession number NG_021282.1). Whether the FAM155B gene product belongs to the NALCN channel complex should be investigated. Also, the mechanisms involved in the regulation of the NALCN channel by CaSR are not known and could imply a co-inclusion in the same protein complex. Some studies indicate genetic interactions between nca-1;nca-2 and other genes in C. elegans. As a matter of fact, null mutants of stomatins (unc-1 and unc-24) and innexins (unc-7 and unc-9) were found to restore the altered sensitivity to GAs and ethanol (Sedensky and Meneely, 1987; Morgan et al., 1990; Morgan and Sedensky, 1995; Sedensky et al., 2001; Humphrey et al., 2007). It was also found that nca-1;nca-2 mutants exhibit a reduced evoked post-synaptic current at the neuromuscular junction and this phenotype is rescued by unc-7 mutants (Bouhours et al., 2011). However, in this latter study, co-immunofluorescent staining experiments do not show obvious neuronal co-localization of UNC-7 and NCA-1/NCA-2. Thus, the reported genetic interaction between unc-7 and nca-1;nca-2 probably reflects a functional, rather than a physical interaction. A genetic interaction was also described between unc-26 null mutants and unc-80 and nca-1;nca-2 (Jospin et al., 2007). unc-26 encodes for Synaptojanin, a lipid phosphatase required to degrade phosphatidylinositol 4,5 bisphosphate (PIP2) at cell membranes during synaptic vesicle recycling (Cremona et al., 1999). It was found that unc-80 and nca-1;nca-2 mutants ameliorate the recycling defects of synaptic vesicles as well as the synaptic transmission in unc-26 null mutants and it was suggested that nca-1;nca-2 function could be regulated by PIP2 (Jospin et al., 2007). Whether Synaptojanin belongs to the NALCN channelosome remains to be investigated. Interestingly, NALCN and PLA2G6 mutations results in similar phenotypes in humans suggesting that iPLA2β could be a regulator of NALCN. Complementary techniques such as two-hybrid and immunoprecipitation studies followed by mass spectrometry approaches will certainly lead to the discovery of new components of the NALCN channelosome.

In addition to their regulation by binding partners, ion channels are known to be post-translationally modulated. For example, ion channel complexes can be phosphorylated by a variety of kinases which regulate their physiological roles (reviewed in Smart, 1997; Dai et al., 2009; Cerda et al., 2011; Scheuer, 2011; Vacher and Trimmer, 2011). An in silico analysis of primary amino-acid sequences of NALCN, UNC-79, UNC-80, and NLF-1 reveals the existence of several predicted phosphorylation sites that are conserved in mammals and for some of these sites, are also conserved in C. elegans, D. Melanogaster, and L. Stagnalis (not shown). The physiological relevance of the NALCN channelosome phosphorylation, as well as the identification of the kinases involved, remains to be explored.

NALCN, and probably UNC-79, UNC-80, and NLF-1, is widely expressed throughout the CNS in mammals (Lee et al., 1999; Swayne et al., 2009; Kang et al., 2011). To better assess the physiological role of NALCN, it is crucial to identify whether expression is restricted to some specific neuronal populations. In addition, NALCN is localized along the axons in C. elegans and in synaptic regions in D. melanogaster (Nash et al., 2002; Humphrey et al., 2007; Yeh et al., 2008), but the neuronal localization of NALCN channel proteins in mammals is unknown. The development of specific antibodies for immunohistochemistry experiments will be crucial for this purpose.

To date, no specific pharmacology for NALCN has been reported and this clearly represents a significant hurdle in the study of NALCN properties and activity. The discovery of specific NALCN agonists/antagonists is critically needed. Such molecules would be tools to investigate NALCN's physiological roles and may also hold interest to develop innovative therapeutic strategies. Whether the NALCN channelosome represents a drug target of interest remains unclear at the present time. Considering that NALCN channel is expressed in pancreatic islets, both in α- and β-cells, and is possibly involved in the regulation of insulin secretion (Kutlu et al., 2009 and http://t1dbase.org; Swayne et al., 2010), targeting NALCN might be relevant to treat type 2 diabetes. It is tempting to speculate that agonists of NALCN would have beneficial effects in pancreatic β-cells on insulin secretion (reviewed in Gilon and Rorsman, 2009). NALCN blockers might be relevant to treat pain considering that SP plays a pivotal role in the transmission of noxious stimuli in the spinal cord (reviewed in Seybold, 2009) and that NALCN is highly expressed in the dorsal spinal cord compared to the ventral spinal cord (Sun et al., 2002). Based on the potential implication of NALCN channel in human physiology and diseases, its pharmacological targeting might also be relevant to treat epilepsy, bowell motility disorders, osmoregulation disorders and psychiatric disorders.

The NALCN channelosome is vital in mammals: homozygous mutant mice exhibit neonatal lethal phenotype and NALCN plays a crucial role in neuronal excitability. NALCN is involved in the regulation of resting membrane potential and is modulated by hormones and neurotransmitters. In the present article, we provide an extensive review of the literature describing the molecular and functional properties of the NALCN channelosome, the various phenotypes observed in NALCN animal models, as well as the recent implications of NALCN in human diseases. We also discuss how NALCN could represent a therapeutic target to treat a wide variety of human diseases, especially neurological and psychiatric diseases. Unfortunately, the lack of specific pharmacology is clearly a brake to all the NALCN investigations. In the coming years, the expected development of selective agonists/antagonists, as well as new animal models, should foster our understanding of the functional properties and physiological roles of the NALCN channelosome.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.