AUTHOR=Lai Mingming , Guo Ye , Ma Jun , Yu Huali , Zhao Dongdong , Fan Wenqiang , Ju Xingda , Sheikh Muhammad A. , Malik Yousra S. , Xiong Wencheng , Guo Weixiang , Zhu Xiaojuan 

TITLE=Myosin X regulates neuronal radial migration through interacting with N-cadherin

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=9

YEAR=2015

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2015.00326

DOI=10.3389/fncel.2015.00326

ISSN=1662-5102

ABSTRACT=<p>Proper brain function depends on correct neuronal migration during development, which is known to be regulated by cytoskeletal dynamics and cell-cell adhesion. Myosin X (Myo10), an uncharacteristic member of the myosin family, is an important regulator of cytoskeleton that modulates cell motilities in many different cellular contexts. We previously reported that Myo10 was required for neuronal migration in the developing cerebral cortex, but the underlying mechanism was still largely unknown. Here, we found that knockdown of Myo10 expression disturbed the adherence of migrating neurons to radial glial fibers through abolishing surface Neuronal cadherin (<italic>N</italic>-cadherin) expression, thereby impaired neuronal migration in the developmental cortex. Next, we found Myo10 interacted with <italic>N</italic>-cadherin cellular domain through its FERM domain. Furthermore, we found knockdown of Myo10 disrupted <italic>N</italic>-cadherin subcellular distribution and led to localization of <italic>N</italic>-cadherin into Golgi apparatus and endosomal sorting vesicle. Taking together, these results reveal a novel mechanism of Myo10 interacting with <italic>N</italic>-cadherin and regulating its cell-surface expression, which is required for neuronal adhesion and migration.</p>