Exosomes as Novel Regulators of Adult Neurogenic Niches

Adult neurogenesis has been convincingly demonstrated in two regions of the mammalian brain: the sub-granular zone (SGZ) of the dentate gyrus (DG) in the hippocampus, and the sub-ventricular zone (SVZ) of the lateral ventricles (LV). SGZ newborn neurons are destined to the granular cell layer (GCL) of the DG, while new neurons from the SVZ neurons migrate rostrally into the olfactory bulb (OB). The process of adult neurogenesis persists throughout life and is supported by a pool of neural stem cells (NSCs), which reside in a unique and specialized microenvironment known as “neurogenic niche”. Neurogenic niches are structured by a complex organization of different cell types, including the NSC-neuron lineage, glial cells and vascular cells. Thus, cell-to-cell communication plays a key role in the dynamic modulation of homeostasis and plasticity of the adult neurogenic process. Specific cell-cell contacts and extracellular signals originated locally provide the necessary support and regulate the balance between self-renewal and differentiation of NSCs. Furthermore, extracellular signals originated at distant locations, including other brain regions or systemic organs, may reach the niche through the cerebrospinal fluid (CSF) or the vasculature and influence its nature. The role of several secreted molecules, such as cytokines, growth factors, neurotransmitters, and hormones, in the biology of adult NSCs, has been systematically addressed. Interestingly, in addition to these well-recognized signals, a novel type of intercellular messengers has been identified recently: the extracellular vesicles (EVs). EVs, and particularly exosomes, are implicated in the transfer of mRNAs, microRNAs (miRNAs), proteins and lipids between cells and thus are able to modify the function of recipient cells. Exosomes appear to play a significant role in different stem cell niches such as the mesenchymal stem cell niche, cancer stem cell niche and pre-metastatic niche; however, their roles in adult neurogenic niches remain virtually unexplored. This review focuses on the current knowledge regarding the functional relationship between cellular and extracellular components of the adult SVZ and SGZ neurogenic niches, and the growing evidence that supports the potential role of exosomes in the physiology and pathology of adult neurogenesis.


INTRODUCTION
It was long believed that mammalian neurogenesis, i.e., the process of generating functional neurons from neural precursors, occurred only during embryonic and perinatal stages ). Altman's pioneering studies in the 1960's provided the first histological evidence for the presence of newborn neurons in the adult brain of rodents (Altman andDas, 1965, 1966;Altman, 1969). However, the fact that young neurons are continuously incorporated into the adult brain circuitry was not widely accepted until the mid-1990's (Gross, 2000;Kaplan, 2001). Advances in immunohistological techniques and particularly the introduction of bromodeoxyuridine (BrdU), a thymidine analog that can be incorporated in vivo, allowed to recognize proliferative populations of cells within the central nervous system (CNS) and to identify the destination of adult-born neurons. To date it is well known that neural stem cells (NSCs) reside in the brain of most adult mammals, including humans, and that neurogenesis does occur throughout life (Eriksson et al., 1998;Gage, 2000;Lie et al., 2004;Abrous et al., 2005;Ming and Song, 2005;Lledo et al., 2006;Merkle and Alvarez-Buylla, 2006;Bergmann et al., 2015;Kempermann et al., 2015). Interestingly, regions harboring active adult neurogenesis are located in discrete but specific areas of the brain. These areas, known as ''neurogenic niches'', are composed of different cell types, specific cell-cell contacts, and particular extracellular cues originated both locally and distantly. Thus, the function of the different cellular and molecular components of the niche supports the physiology of NSCs, balancing quiescence with proliferation, and regulating cell differentiation (Conover and Notti, 2008).
In this context, not only the cytoarchitectonic organization of the niche but also the ways of communication between the cellular components, are critical to understand the adult neurogenic process under both physiological and pathological conditions. Cells communicate reciprocally with other cells by (i) intercellular contacts, and (ii) secreted molecules, such as growth factors, cytokines, hormones, etc. (paracrine or endocrine communication). However, a novel way of cell-to-cell communication mediated by extracellular vesicles (EVs) has attracted the attention of several researchers in different fields. EVs, such as exosomes, carry a specific cargo of proteins, lipids and nucleic acids and are currently consider one of the most complex and physiologically relevant messengers between cells. This review focuses on the cellular components of the adult neurogenic niches, the mechanisms involved in intercellular communication, and the potential role of exosomes as regulators of the neurogenic process, and as mediators and novel biomarkers of neuropsychiatric and neurological disorders associated with defective adult neurogenesis.

ADULT NEUROGENIC NICHES
In the adult mammalian brain, neurogenesis is well documented to continue throughout life in two regions: the subventricular zone (SVZ) of the lateral ventricles (LV) and the subgranular zone (SGZ) of the dentate gyrus (DG) in the hippocampus ( Figure 1A). A lineage model from NSCs to mature neurons has been characterized in both, the SVZ and the SGZ adult neurogenic niches ( Figure 1B). In this model, NSCs give rise to neural progenitor cells (NPCs), also known as transit amplifying cells because of their limited division potential, which differentiate into migrating neuroblasts and then, into neurons that integrate into pre-existing circuits (Gage, 2000). Neuroblasts in the SGZ migrate short distances and integrate into the existing circuitry of the granular cell layer (GCL) of the DG ( Figure 1C); those from the SVZ migrate along the rostral migratory stream (RMS) and supply newborn neurons for the olfactory bulb (OB; Figures 1A,D). Interestingly, glial and vascular cells are also major contributors to the configuration of functionally structured neurogenic niches (Figures 1B-D). Growing evidence suggests that the adult mammalian brain contains other neurogenic niches that are capable of generating new neurons and glial cells, particularly after injury or after some inductive stimuli (Lin and Iacovitti, 2015). The neocortex (Gould et al., 1999;Gould, 2007;Cameron and Dayer, 2008) and the hypothalamus (Kokoeva et al., 2005) have also been reported to support adult neurogenesis, but the magnitude of the neurogenic process in these regions is still under debate. Although the functional significance of adult-born neurons under physiological and/or pathological conditions has not been completely clarified and is being actively pursued, it is well-defined that neurogenesis in the SVZ and SGZ of the adult brain depends on the presence and maintenance of NSCs, which is tightly regulated by their highly specialized microenvironments or neurogenic niches (Palmer et al., 2000;Alvarez-Buylla and Lim, 2004;Ma et al., 2005;Merkle and Alvarez-Buylla, 2006).
Niches are defined by their ability to anatomically house stem cells and functionally control their development in vivo (Ma et al., 2008). The concept that stem cells reside within specific niches was first suggested in the 1970's (Schofield, 1978), but it was not until the 2000's, when substantial progress was made in describing both the cellular components of the niches and their functional interactions, in several mammalian tissues, including skin, intestine and bone marrow (Spradling et al., 2001;Li and Xie, 2005;Scadden, 2006). In the adult brain, much is known about the cellular composition and organization that characterize the SVZ and SGZ neurogenic niches (Ma et al., 2008;Mirzadeh et al., 2008;Aimone et al., 2014;Bjornsson et al., 2015;Licht and Keshet, 2015). Furthermore, the interaction and functional coordination of these components as well as the heterogeneity and complexity of neurogenic niches and their emerging roles under pathological conditions is being pictured (Jordan et al., 2007;Alvarez-Buylla et al., 2008).
FIGURE 1 | Neurogenic niches in the adult mammalian brain. (A) Schematic representation of the neurogenic regions (niches) in a sagittal section of the adult mouse brain: the subgranular zone (SGZ, orange) in dentate gyrus (DG) of the hippocampus (Hip), and the subventricular zone (SVZ, red) in the lateral wall of the lateral ventricles (LV). SVZ-derived newborn neurons migrate towards the olfactory bulb (OB) through the rostral migratory stream (RMS). (B) Cellular components of neurogenic niches. In addition to the neural stem cell (NSC)-neuron lineage, neurogenic niches are composed of glial cells (astrocytes, microglia and ependymal cells) and vascular cells (endothelial cells, pericytes). (C,D) Illustration of SGZ and SVZ neurogenic niches. The cytoarchitecture and relationships between cellular components of the niche are represented. Different cell types (color and shape) correspond to those depicted in (B). Note the close proximity between blood vessels and NSCs/NPCs in both niches. (C) The SGZ neurogenic niche. Radial type 1 cells correspond to the NSCs that give rise to type 2a/b NPCs, which differentiate into type 3 neuroblasts. Neuroblasts migrate guided by astrocytes and become maturing neurons that finally mature and integrate into the granular cell layer (GCL). (D) SVZ neurogenic niche. This niche is located underneath the ependymal lining (E) of the LV. It is composed of type B quiescent cells (NSCs), which can activate and generate type C NPCs that rapidly proliferate and generate type A neuroblasts. Neuroblasts migrate long distances through the rostral migratory stream (RMS) to the OB where they mature into interneurons. Note that a tunnel of astrocytes and a scaffold of blood vessels guide migration of neuroblasts. Also note that monociliated type B cells can directly contact cerebrospinal fluid (CSF) and blood vessels. Ce, cerebelum; Cx, cortex; Str, striatum.
cerebrospinal fluid (CSF)-filled ventricles and on the other by a complex arrangement of parallel blood vessels (Mirzadeh et al., 2008;Shen et al., 2008; Figure 1D). NSCs that reside in the SVZ, also known as Type B cells, exhibit hybrid characteristics of astrocytes (GFAP+) and immature progenitors (S100β+, Nestin+, Sox2+; Kriegstein and Alvarez-Buylla, 2009). Type B cell bodies are typically located under the ependymal lining of the LV and some of them have a short apical process with a single primary cilium that projects through the ependymal cell layer to contact the CSF directly, and a basal process that ends on the blood vessels of the SVZ plexus (Mirzadeh et al., 2008). Interestingly, apical processes of various type B cells form bundles at the center of a ''pinwheel'' of ependymal cells (Mirzadeh et al., 2008). As a result of their position and polarized phenotype, type B cells are strategically located to receive cues from both the vascular and the CSF compartments ( Figure 1D). Quiescent type B cells can eventually divide asymmetrically to give rise to type C (Mash1+) transit-amplifying progenitor cells (Doetsch et al., 1997;Merkle and Alvarez-Buylla, 2006). Most of type C cells, in turn, divide to give rise to PSA-NCAM+ neuroblasts (type A cells). Type A cells form clusters and chains that migrate toward the OB guided by a channel of astrocytes and by a parallel scaffold of blood vessels. The anatomical structure formed by migrating (type A) neuroblasts is known as the RMS. Within the OB, these immature neurons differentiate into two types of GABAergic interneurons: the granular neurons and the periglomerular neurons, which integrate into the existing neuronal circuitry (Merkle and Alvarez-Buylla, 2006;Curtis et al., 2007;Kriegstein and Alvarez-Buylla, 2009; Figures 1A,D). Interestingly, type B/C cells can also originate glia (oligodendrocytes or astrocytes; Menn et al., 2006).

The Subgranular Zone (SGZ) Niche
The SGZ is a region located beneath the GCL of the DG of the hippocampus ( Figure 1C). The NSCs to mature neurons lineage model described in the SVZ can be comparably applied in the SGZ. A similar subset of GFAP+/Sox2+/Nestin+ radial glia-like cells are also believed to be quiescent NSCs of the SGZ (Seri et al., 2001). These NSCs, also known as type 1 cells, give rise through asymmetric division to transit-amplifying non-radial progenitors (Nestin+/GFAP−) or type 2a cells. Type 2a cells subsequently originate what appears to be a more fate-committed (Tbr2+) intermediate progenitor (type 2b) cell population, which give rise to doublecortin (Dcx) + (type 3) neuroblasts. Finally, neuroblasts differentiate into maturing glutamatergic granule cells that migrate, guided by astrocytic processes, and integrate in the GCL of the DG (Aimone et al., 2014; Figure 1C).

CELLULAR COMPONENTS OF THE NEUROGENIC NICHE AND THEIR ROLE IN THE NEUROGENIC PROCESS
The highly hierarchical NSC-neuron lineage in both the SVZ and SGZ requires the precise regulation of NSCs self-renewal, fate specification, maturation and integration of new neurons in the existing neural circuitry. The convergence of several cellular and extracellular factors contributes to building a unique and specialized niche or microenvironment that regulates the physiology of NSCs during the course of adult life. Therefore, the identification and functional characterization of these factors emerges as a key aspect not only to better understand the biology of adult NSCs but also to develop novel therapies for a number of neurological and psychiatric disorders associated with defects in adult neurogenesis. Significant advances have been made in the description of the cellular components of the neurogenic niches and the mechanisms by which they can individually or coordinately contribute in regulating adult neurogenesis (reviewed in Ma et al., 2008;Aimone et al., 2014;Bjornsson et al., 2015; Figure 2).

Astrocytes
Astrocytes represent one of the major contributors to the neurogenic niche (Song et al., 2002). Co-culture experiments with astrocytes isolated from the adult hippocampus and cortex, but not from the spinal cord, induce neuronal differentiation of NPCs (Barkho et al., 2006;Oh, 2010). Furthermore, hippocampus-derived astrocytes are more efficient than cortical astrocytes in promoting neuronal fate of NPCs (Oh, 2010), suggesting that the functional heterogeneity of astrocytes may be reflecting unique characteristics of different brain regions or environments. Interestingly, they may exert either positive or negative regulatory roles in the neurogenic process (Figure 2). Astrocytes negatively control neuronal differentiation of neural stem/progenitor cells through cell-cell contacts in which Jagged1-mediated Notch pathway and intermediate filament proteins GFAP and vimentin play a significant role (Wilhelmsson et al., 2012). Similarly, some astrocyte-secreted factors such as insulin-like growth factor binding protein 6 (IGFBP6) and decorin inhibit neuronal differentiation of adult NSCs/NPCs (Barkho et al., 2006). On the other hand, positive effects on neuronal differentiation of adult SGZ NSCs involve cell-cell contact by ephrin-B2(+) astrocytes (Ashton et al., 2012). Likewise, astrocytic ATP release positively regulates proliferation of NSCs in the SGZ (Cao et al., 2013) and astrocyte-derived soluble factors such as Wnt3 (Seri et al., 2001;Lie et al., 2005), neurogenesin-1 (Ueki et al., 2003), thrombospondin-1 (Lu and Kipnis, 2010) and interleukins such as IL-1β, IL-6 ( Barkho et al., 2006), promote hippocampal neurogenesis. In agreement with a positive regulatory action of astrocytes, agerelated changes in astrocyte population, including the decline of FGF-2 + astrocytes, correlate with low levels of neurogenesis in the aged hippocampus (Shetty et al., 2005).
Astrocytes also contribute to the migration of neuroblasts along the RMS by creating a physical route or tube where they (i) communicate with migrating neuroblasts (Bolteus and Bordey, 2004), and (ii) help to maintain the architecture of the vasculature scaffold in a VEGF-and thrombospondin-4dependent fashion (Bozoyan et al., 2012;Girard et al., 2014). Similarly, in the adult SGZ, astrocytic radial processes facilitate the short-distance migration of newly generated neurons from the SGZ to the GCL of the DG (Shapiro et al., 2005). In addition, adult hippocampal astrocytes promote maturation and synaptic integration of the neural progeny of NSCs in co-culture experiments (Song et al., 2002).

Ependymal Cells
Mature ependymal cells form a simple cuboidal to low columnar epithelium-like structure lining ventricular cavities. These cells have microvilli and tufts of motile cilia that contribute to the CSF hydrodynamic flow (Nelson and Wright, 1974;Ibañez-Tallon et al., 2004;Spassky et al., 2005). Disruption of ependymal lining or ciliary defects can provoke CSF flow disturbances and hydrocephalus (Jiménez et al., 2001;Wagner et al., 2003; FIGURE 2 | Distinct roles of niche cells on NSC-neuron lineage at different stages/steps of adult neurogenesis. Best-described cell-cell communication mechanisms in neurogenic niches rely on soluble mediators or direct contact between the signaling and the targeting cells. The gradient bars represent varying influence of secreted factors or cell-cell contacts on (i) activation of quiescent NSCs (proliferation); (ii) differentiation or fate specification; (iii) migration; and (iv) survival/maturation of newborn neurons. Green: stimulation or activation; Red: inhibition; Yellow: activation or inhibition according to the circumstances. For details and references, see the text. ATP, adenosine triphosphate; BDNF, brain-derived neurotrophic factor; CXCL, chemokine (C-X-C motif) ligand; FGF, fibroblast growth factor; GDNF, glial cell line-derived neurotrophic factor; GFAP, glial fibrillary acidic protein; HGF, hepatocyte growth factor; IGF, insulin-like growth factor; IGFBP, insulin-like growth factor binding protein; IL, interleukin; NGF, nerve growth factor; NT, neurotrophin; PEDF, pigment epithelium-derived factors; SDF, stromal cell-derived factor; TGF, transforming growth factor; TNF, tumor necrosis factor; TSP, thrombospondin; VEGF, vascular endothelial growth factor. -Tallon et al., 2004;Banizs et al., 2005;Town et al., 2008). Because of their location, ependymal cells display certain barrier and signaling functions between CSF and neural tissue (Sarnat, 1992;Del Bigio, 1995). Ependymal cells are joined together at their apical regions by N-cadherin-based adherens junctions (Brightman and Reese, 1969;Del Bigio, 1995;Rodríguez and Bouchaud, 1996) and blocking N-cadherin function in vitro induces apoptosis of ependymal cells and denudation of ventricular walls (Oliver et al., 2013). Ependymal denudation of ventricular walls increases with aging (Luo and Craik, 2008) and is associated with the pathogenesis of neurodevelopmental disorders such as periventricular heterotopia (Ferland et al., 2009), spina bifida aperta (Sival et al., 2011) and hydrocephalus (Jiménez et al., 2009;Wagner et al., 2003;Domínguez-Pinos et al., 2005). Comprehensive analyses by Spassky et al. (2005) indicate that most ependymal cells are born at embryonic stages and that differentiated ependymal cells are postmitotic. However, it has been suggested that ependymal cells can be generated postnatally in certain brain regions . Furthermore, it has been argued that ependymal cells lining the SVZ can act as NSCs (Chojnacki et al., 2009). Interestingly, pathological stimuli such as stroke can stimulate forebrain ependymal cells to proliferate and generate neurons and astrocytes (Carlén et al., 2009).

Ibañez
Beyond this controversy, it is well defined that ependymal cells are a key component of the SVZ neurogenic niche. They are a source of noggin and pigment epithelium-derived factor (PEDF; Figure 2). Noggin is a bone morphogenetic protein (BMP) antagonist involved in the maintenance of a neurogenic niche (Lim et al., 2000). Opposite results have suggested that BMP signaling can inhibit adult neurogenesis (Lim et al., 2000), or promote it (Colak et al., 2008). Thus, the role of ependymal-derived noggin on the SVZ physiology is still not clear. PEDF is secreted by both ependymal and endothelial cells, and specifically participates in the self-renewal capacity of type B cells of the SVZ, thereby maintaining a pool of undifferentiated NSCs in the neurogenic niche (Ramírez-Castillejo et al., 2006). Furthermore, it is known that PEDF modulates stemness of NSCs by activating Notch-dependent transcription in these cells (Ramírez-Castillejo et al., 2006;Andreu-Agullo et al., 2009). In this context, the loss or disruption of the ependyma in the SVZ neurogenic niche would impair ependymal cell-mediated signaling pathways and alter its neurogenic potential. This appears to be the case in the SVZ niche of the hyh mutant mice where ependymal cells of the LV are completely lost during early postnatal stages and proliferation of SVZ progenitors is dramatically reduced (Jiménez et al., 2009). Interestingly, ependymal-denuded surfaces become progressively covered by a layer of astrocytes that acquire certain morphological and antigenic ependymal cell-like properties and probably contribute to the repair of the ependymal lining (Páez et al., 2007;Roales-Buján et al., 2012). However, the presence of a reactive astroglial ''scar'' can reduce the proliferative activity of SVZ neural progenitors, blocking neuronal regeneration and revascularization and thus, interfering with the recovery of an injured area (Fawcett and Asher, 1999;Kernie et al., 2001).

Microglia
Most microglial cells of the CNS are already generated by the end of the second postnatal week (Ginhoux et al., 2013). In the adult brain, resting microglial cells represent the resident macrophages and they survey the brain parenchyma (Nimmerjahn et al., 2005). In the SGZ of the hippocampus, microglial cells have a prominent surveillant and phagocytic role. It has been demonstrated that most newborn granule neurons of the adult SGZ undergo apoptosis and are phagocytosed by microglial cells (Sierra et al., 2010(Sierra et al., , 2013. Beyond their role as brainś professional phagocytes, microglial cells can also serve neuroprotective or neurotoxic roles depending on the physiological and pathological circumstances (Luo and Chen, 2012;Hellwig et al., 2013;Brites and Vaz, 2014). In addition, microglial cells can interact with NSCs/NPCs (Su et al., 2014) and regulate adult neurogenesis by secreting several soluble mediators, such as growth factors and cytokines, that influence either positively or negatively the neurogenic process (reviewed in Kim and de Vellis, 2005;Harry, 2013; Figure 2). In vitro experiments suggest that microglia secret factors that promote neuronal differentiation of SVZ-derived NSCs but not their maintenance or self-renewal (Walton et al., 2006). On the other hand, activation of microglial cells can result in negative regulatory actions on the SGZ neurogenic process (Sierra et al., 2014). For example, inflammatory or LPS-mediated activation of microglia can inhibit neurogenesis and favor gliogenesis and this effect is partially mediated by the secretion of proinflammatory cytokines, such as TNF-α (Monje et al., 2003;Butovsky et al., 2006;Carpentier and Palmer, 2009). On the other hand, when microglial cells are stimulated with interleukin-4 (IL-4) and interferon-γ, they secrete insulin-like growth factor-1 (IGF-1) and induce neuronal differentiation of neural progenitors (Butovsky et al., 2006). Additionally, the environmental context of the neurogenic niche determines not only the mode of activation of microglial cells and the positive or negative regulatory actions of these cells as a population but also defines the pro-or anti-neurogenic properties of specific microglial-secreted cytokines, such as the transforming growth factor β (TGF-β; Battista et al., 2006;Douglas-Akinwande et al., 2006). Interestingly, physical exercise (running)-induced cell proliferation and neurogenesis in the SGZ (van Praag et al., 1999b;Aimone et al., 2014) is associated to a decrease in microglial function (Olah et al., 2009;Vukovic et al., 2012;Gebara et al., 2013). Inversely, age-related changes in microglia activity, including an increase in their reactive profile with higher secretion of pro-inflammatory cytokines (Njie et al., 2012), potentially contribute to the decline of neurogenesis seen with aging (Gemma et al., 2010;Gebara et al., 2013).

Endothelial Cells and Pericytes
The close physical proximity of NSCs/NPCs with blood vessels within both adult neurogenic niches suggests that critical factors derived from the vasculature may act as major modulators of the neurogenic process and consequently, several authors consider the SVZ and the SGZ as ''vascular niches'' (Palmer et al., 2000;Shen et al., 2008;Tavazoie et al., 2008;Licht and Keshet, 2015). Interestingly, vascularderived signals or messengers can be originated distantly, in peripheral organs (blood-borne substances), and/or locally from endothelial cells and pericytes (paracrine communication). Endothelial cells are able to secrete several factors that modulate adult neurogenesis (Palmer et al., 2000;Shen et al., 2004; Figure 2). Several studies suggest that endothelial cell-produced brain derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) stimulates SGZ neurogenesis both in vivo and in vitro (Jin et al., 2002;Cao et al., 2004;Kim et al., 2004). Interestingly, endothelial cells and NSCs reciprocally influence each other to couple SGZ neurogenesis to angiogenesis, and it is suggested that VEGF is critical to coordinate these processes (Palmer et al., 2000;Riquelme et al., 2008;Udo et al., 2008;Ruiz de Almodovar et al., 2009). Furthermore, VEGF appears to be necessary for exercise-induced SGZ neurogenesis (Fabel et al., 2003). Together with ependymal cells, endothelial cells secrete PEDF and thus, stimulate self-renewal of NSCs (Ramírez-Castillejo et al., 2006;Andreu-Agullo et al., 2009). Neurotrophin-3 (NT3) and betacellulin, secreted by vascular endothelial cells of both the SVZ plexus and the choroid plexus, regulate SVZ neurogenesis by different mechanisms. NT3 helps to maintain the quiescence of NSCs (Delgado et al., 2014), whereas betacellulin promotes proliferation of NPCs and neuroblasts (Gomez-Gaviro et al., 2012). Vascular endothelial cells also secrete a chemokine known as CXCL12 or stromal derived factor-1 (SDF1) that differentially modulates SVZ NSC-neuron lineage (Kokovay et al., 2010). In type B and type C cells, SDF1 upregulates EGFR and alpha-6 integrin, activating and attracting them to the blood vessels while SDF1 stimulates motility of type A neuroblasts (Kokovay et al., 2010).
The role of pericytes in adult neurogenic niches is less well characterized. Located in intimate contact with endothelial cells, they can act as regulators or transducers of both blood-circulating signals and endothelial-derived factors (Armulik et al., 2011). Pericytes secrete TGF-β, a known modulator of adult neurogenesis and blood-brain barrier (BBB; Dohgu et al., 2005). They also secrete neurotrophins in response to hypoxic conditions (Ishitsuka et al., 2012). In addition to their potential paracrine function, it has been demonstrated that pericytes remain relatively undifferentiated, retaining the capacity to differentiate into several cell types, including neural-related progeny. Consequently, it has been proposed that pericytes may act as NSCs under certain circumstances (Dore-Duffy et al., 2006;Dore-Duffy and Cleary, 2011). On the other hand, pericytes might be involved in the pathogenesis of different CNS diseases and have been proposed as a potential therapeutic target (Lange et al., 2013).

Neurotransmitters (Innervation)
Numerous neuromodulatory systems have been shown to affect proliferation and differentiation of NSCs/NPCs and the maturation of adult-born neurons. Adult SVZ receives inputs from dopaminergic projections from the ventral tegmental area (VTA) and the substantia nigra (Baker et al., 2004). It has also been shown that SVZ type C cells express D2-like dopaminergic receptors (Höglinger et al., 2004;Kippin et al., 2005). However, the effects of dopamine on adult NPCs are controversial and it has been suggested that dopamine differentially affects type B and C cells via a distinctive mix of receptors in each cell type (Berg et al., 2013). On the other hand, dopamine D2 receptor-induced neurogenesis appears to be mediated by ciliary neurotrophic factor (CNTF), a mitogen expressed by astrocytes that is upregulated by D2 agonists (Yang et al., 2008). The role of dopamine in the SGZ neurogenic process is less well understood (Veena et al., 2011) but it has been suggested that, similarly to the SVZ, D2 receptors are involved in hippocampal neurogenesis in vivo (Yang et al., 2008). Other major neuromodulatory systems include the serotoninergic and cholinergic systems. The role of serotonin in SGZ neurogenesis has been deeply studied because of its link to depressive-like behaviors (Gould, 2007). The DG receives serotoninergic inputs from the median and dorsal raphe nuclei (Leranth and Hajszan, 2007). Interestingly, it is well documented that fluoxetine, a serotonin-reuptake inhibitor widely used as antidepressant, increases the proliferation rates in the hippocampal SGZ (Malberg et al., 2000). Similarly, serotonergic axons originated in the raphe nuclei form a widespread network at the ventricular surface of the LV and contact type B NSCs of the adult mouse SVZ. Activation of serotonin receptors of Type B cells increases proliferation (Tong et al., 2014). Mouse SVZ NSCs/NPCs also received inputs from choline acetyltransferasepositive axons originating in the SVZ itself. The release of acetylcholine by these neurons depends on their activity and can directly control SVZ proliferation (Paez-Gonzalez et al., 2014).
In addition, local or regional network neurotransmitters also play a significant role in adult neurogenesis. It has been demonstrated that non-synaptic GABA signaling (released by type A neuroblasts) can regulate proliferation of type B NSCs in the SVZ by activating GABA A receptors. This pathway provides a feedback mechanism to control the balance between self-renewal and differentiation of NSCs (Liu et al., 2005). Interestingly, this mechanism can also be reinforced by other SVZ GABAergic inputs such as the striatal GABAergic neurons (Young et al., 2014). In the SGZ niche, GABA helps to maintain the quiescence of radial glia-like (type 1) cells through activation of γ2-subunit containing GABA A receptors (Song et al., 2012). Similarly to the SVZ, these GABAergic inputs appear to be non-synaptic and due to diffusion from nearby parvalbumin+ basket cells synapses (Song et al., 2012). On the other hand, type 2 NPCs and young neurons receive more direct GABAergic inputs (Ge et al., 2006;Markwardt et al., 2009). In the adult DG, maturing neurons also receive glutamatergic inputs and it has been shown that NMDA receptor activation is critical for the survival and integration of young neurons (Tashiro et al., 2006).

CSF-Derived Factors
The SVZ is uniquely situated to experience the effects of the flow and the composition of the CSF. Intriguingly, the direction of migrating neuroblasts in the RMS, parallels that of CSF flow (Sawamoto et al., 2006). Furthermore, when ependymal ciliary movements and CSF flow are disrupted, neuroblasts become disoriented (Sawamoto et al., 2006). On the other hand, the CSF contains several compounds that participate in the neurogenic process (Lafon-Cazal et al., 2003;Bunn et al., 2005;Redzic et al., 2005;Zappaterra and Lehtinen, 2012). Transcriptome analyses of adult and embryonic choroid plexus (ChP) have revealed that ChP epithelial cells express several growth factors and signaling molecules that can act either as positive (TGF-α, amphiregulin, IGF2, and FGF2) or negative (TGF-β superfamily members) regulators of adult neurogenesis (Marques et al., 2011;Liddelow et al., 2012). They also secrete SLIT1/2, known chemorepulsive signals that help type A neuroblasts to migrate in the RMS (Nguyen- Ba-Charvet et al., 2004). Other factors, such as ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF), play important roles in promoting proliferative activity and maintenance of undifferentiated neural progenitors (Shimazaki et al., 2001;Gregg and Weiss, 2005). Recently, it was found that two Gprotein coupled receptor (GPCR) ligands present in the adult CSF, namely sphingosine-1-phosphate (S1P) and prostaglandin-D2 (PGD2), promotes quiescence of SVZ NSCs (Sato et al., 2007;Kondabolu et al., 2011;Codega et al., 2014). The role of the ChP/CSF-mediated signaling pathway has been highlighted in aged and hydrocephalic animal models. Agingrelated changes in the ChP/CSF system negatively affect adult neurogenesis (Baruch et al., 2014). Additionally, it has been demonstrated that the composition of the CSF is altered in hydrocephalus (Mashayekhi et al., 2002;Owen-Lynch et al., 2003) and hydrocephalic CSF inhibits cell proliferation cortical cells cultures, suggesting that CSF composition modifies some of the properties of NPCs (Owen-Lynch et al., 2003). In fact, the altered composition of CSF affects the normal cortical development in hydrocephalic H-Tx embryos . However, the specific CSF changes that mediate these effects are actually not known, and the influences of the hydrocephalic CSF on the SVZ neurogenic niche have not yet been reported.

Blood-Borne Substances
As stated before, the vasculature can influence NSCs proliferation and differentiation by providing signaling molecules secreted locally by endothelial cells and pericytes, as well as by providing systemic blood-circulating factors (Tavazoie et al., 2008;Egeland et al., 2015;Licht and Keshet, 2015). Blood-borne factors that influence adult neurogenesis, including hormones, cytokines, metabolites, and gases can gain access to the SVZ neurogenic niche either via the ChP-CSF pathway route or more directly via the SVZ vasculature (Egeland et al., 2015;Licht and Keshet, 2015). In both cases, the presence of barriers must be considered. Indeed, ChP epithelial cells are joined together by tight junctions and constitute the blood-CSF barrier (BCB) while the presence of tight junctions between brain parenchymal endothelial cells constitute the BBB (Engelhardt and Sorokin, 2009). It has been suggested that SVZ vessels have a looser BBB than other brain vessels, allowing circulating substances to modulate adult neurogenesis. In this context, Tavazoie et al. (2008) have demonstrated that some circulating fluorescent tracers have better access to the brain parenchyma through SVZ vessels than through vessels located in other cerebral regions. Furthermore, NSCs/NPCs can directly contact blood vessels at specialized sites that lack pericyte coverage and glial end feet, a feature unique to SVZ vascular plexus (Tavazoie et al., 2008).
Several hormones seem to modulate adult neurogenesis under particular conditions. For example, prolactin can enhance SVZ neurogenesis in pregnant mice (Shingo et al., 2003), while increased levels of glucocorticoids associated with stress have the opposite effect (Snyder et al., 2011). In turn, dietary restriction and exercise/enriched environment positively modulate neurogenesis and it is proposed these effects are, at least in part, consequence of changes in the composition of blood-borne substances (van Praag et al., 1999a;Wu et al., 2008). Interestingly, the age-related decline of the neurogenic niche could be restored by extrinsic young signals. Using a mouse heterochronic parabiosis model, Katsimpardi et al. (2014) showed that (i) aged cerebral vasculature is remodeled, and (ii) SVZ neurogenesis is re-activated in response to young systemic factors. Furthermore, the authors revealed that GDF11, a circulating TGF-β family member, participate in this process (Katsimpardi et al., 2014). These examples emphasize the fact that the adult neurogenic niche communicates with the systemic circulation and highlight the role of blood-borne substances in the modulation of adult neurogenesis.
Interestingly, in addition to the well-recognized soluble signals originated both locally by the cellular components of the adult neurogenic niches (reviewed in ''Cellular components of the neurogenic niche and their role in the neurogenic process'' Section), or distantly (reviewed in ''Distant regulation of the neurogenic niche: neurotransmitters, cerebrospinal fluid-derived factors, and blood-borne substances'' Section), novel types of intercellular messengers, such as EVs, have emerged. EVs, and particularly exosomes, are considered one of the most complex ways of cell-to-cell communication, allowing the transfer of mRNAs, microRNAs (miRNAs), proteins and lipids between cells and thus, being able to modify the physiology of recipient cells. However, their precise role in adult neurogenic niches remains virtually unexplored.

EXOSOMES: UNIQUE MESSENGERS FOR CELL-TO-CELL COMMUNICATION
EVs, and particularly exosomes, are emerging as one of the major mediators of intercellular communication. It is known since the late 1960's/early 1970's that membrane-enclosed vesicles are present outside cells in different tissues and biological fluids (Colombo et al., 2014). Originally, these EVs were thought to be released only by outward budding or shedding of the plasma membrane (PM) but 10 years later, it was described that vesicles contained within the lumen of so-called multivesicular endosomes or multivesicular bodies (MVBs) could be secreted (Harding and Stahl, 1983;Pan and Johnstone, 1983). Thus, the term ''exosome'' was introduced to identify those EVs that have been originated from the endosomal system (Johnstone et al., 1987) and since then, the field of exosome research has expanded exponentially. Now it is known that various types of EVs coexist in the extracellular milieu. Some of them are originated from shedding of the PM (microvesicles and apoptotic bodies) and others (exosomes) are secreted by exocytosis after fusion of MVBs with the PM. Thus, the various intracellular origins and modes of formation can probably lead to different compositions and functions of EVs (Kowal et al., 2014). Exosomes represent one sub-type of these EVs and a lot of progress has been made in the last few years to understand the basic mechanisms by which exosomes are formed and secreted, and their function in cell-cell communication under physiological and pathological conditions.

Biogenesis of Exosomes
Exosomes are small membrane vesicles, 30-100 nm in size, that are secreted by almost all cell types. They originate in the endocytic pathway, which is involved in the trafficking of several proteins that are internalized and can either recycle back to the PM or get sorted to degradation (Gould and Lippincott-Schwartz, 2009;Klumperman and Raposo, 2014) In these pathways, early endosomes mature into late endosomes (LE), and during this process, they suffer an inward budding process of their membrane and accumulate intraluminal vesicles (ILVs; Figure 3A). Because of their mature morphological features, LEs are referred to as MVBs. In most cells, the main fate of MVBs is to fuse with lysosomes, ensuring the degradation of their content. However, organelles with hallmarks of MVBs, can also fuse directly with the PM, releasing ILVs into the extracellular milieu (Raposo et al., 1996;Jaiswal et al., 2002; Figure 3A). To date is still unknown how different subpopulations of MVBs coexist within cells and whether these different populations follow the same or different routes such as fusion with lysosomes for degradation or fusion with PM for exocytosis (Edgar et al., 2014). The best-described mechanism for the formation of MVBs and ILVs is driven by the endosomal sorting complexes required for transport (ESCRT) machinery. This machinery is composed of approximately thirty proteins that assemble into four complexes (ESCRT-0, -I, -II and -III) with associated proteins (VPS4, VTA1, ALIX also called PDCD6IP) conserved from yeast to mammals (Hanson and Cashikar, 2012). The ESCRT-0 complex recognizes and sequesters ubiquitinated transmembrane proteins in the endosomal membrane, segregates them into microdomains and binds the ESCRTI complex. ESCRTI, in turn, recruits ESCRTII subunits, and then both complexes initiate membrane deformation into buds with sorted cargo, allowing cytosolic proteins and RNAs (including mRNAs and miRNAs) to get into the forming vesicles. Following, the ESCRTII complex recruits ESCRTIII subunits inside nascent vesicles and together with the ATPase VPS4, provoke the membrane cleavage required to free ILVs while ubiquitin molecules and ESCRT subunits are released into the cytosol for their recycling (Hanson and Cashikar, 2012). Recently, alternative ESCRT-independent mechanisms have been proposed for the formation and sorting of specific cargo into exosomes, including a lipid-driven mechanism in which the synthesis of ceramide by a neutral sphingomyelinase is involved (Trajkovic et al., 2008) and a sphingosine 1-phosphate receptordependent mechanism (Kajimoto et al., 2013). Alternatively, ALIX, an ESCRTIII binding protein, by interacting with proteins containing late-domain motif LYPXnL, such as syntenin or the GPCR Par1, can be sorted into exosomes by a mechanism that requires only the recruitment of the ESCRTIII subunit CHMP4 (Sette et al., 2010;Baietti et al., 2012;Hurley and Odorizzi, 2012).

Content and Extracellular Fate of Exosomes
Considering the mechanisms involved in the biogenesis of ILVs, a complex mix of proteins, lipids and other cytosolic molecules is specifically sorted and incorporated into ILVs/exosomes.The content of exosomes is currently compiled in databases named Exocarta 1 , Vesiclepedia 2 and EVpedia 3 which are continuously updated by the scientific community (Mathivanan et al., 2012). According to the current version of Exocarta, 9769 proteins, 1116 lipids, 3408 mRNAs, and 2838 miRNAs have been identified in exosomes from many different cell types and organisms. Much effort has been made to establish the proteomics of exosomes. Initially, it was found that exosomes contain proteins also present in endosomes, the PM, and the cytosol, but few components from other organelles such as nucleus, mitochondria or Golgi, confirming that they are not composed of a random set of proteins (Théry et al., 2001). The most frequently identified proteins in exosomes includes (i) proteins involved in MVB biogenesis; (ii) membrane associated proteins (lipid rafts proteins, GTPases and other membrane trafficking proteins); (iii) transmembrane proteins (targeting/adhesion molecules, tetraspanins (CD9, CD63, CD81), membrane fusion proteins); (iv) cytoskeletal proteins (actin, syntenin, moesin); (v) signal transduction proteins (annexin, 14-3-3 proteins); (vi) chaperones (Hspa8, Hsp90); and (vii) metabolic enzymes (GAPDH, LDHA, PGK1, aldolase, PKM), among others (Chaput and Théry, 2011;Mathivanan et al., 2012; Figure 3B). Interestingly, and even though there is an important overlap in terms of protein composition with other EVs, many of these proteins, such as tetraspanins, CD63 and CD81, are considered exosome markers.
In addition, exosomes contain a specific lipid composition (Kowal et al., 2014): they are enriched in ceramide, cholesterol, sphingomyelin, and phosphatidyl-serine. Interestingly, they are not enriched in lysobisphosphatidic acid, a lipid described in ILVs (Matsuo et al., 2004). As stated before, some of these lipids can be involved in the formation of ILVs or their release outside the cell. One very striking aspect of the content of exosomes is that they are highly enriched in small (no longer than 200 nucleotides) non-coding RNAs (ncRNAs). The most widely described exosome-associated ncRNAs are the miRNAs  (ii) ESCRT-independent (lipid-driven) mechanisms, which depends on the presence of ceramide and neutral sphingomyelinase. Then, a population of MVBs is destined to degradation into lysosomes (Lys) and another population of MVBs fuse with the plasma membrane (PM), thus allowing ILVs to be exocytosed as exosomes. Once in the extracellular space, secreted exosomes can act as local signals (paracrine communication) or travel through biological fluids (e.g., blood, CSF) to reach body sites located distant from their cell/tissue of origin. Exosomes interact with recipient/target cells in several ways: (i) they can activate surface receptors (ligand/receptor interaction); (ii) they can transfer exosomal cargo to recipient cells by membrane fusion or by a connexin-dependent mechanism; and (iii) they can be endocytosed by a macropinocytic mechanism and then fuse with endosomal membranes to transfer their cargo. (B) Exosomes are small (30-100 nm) membrane bound vesicles with a complex and functionally relevant composition. They contain nucleic acids (mRNA and miRNA), and a vast array of different proteins and lipids depending on their host cell. However, they are generally enriched in proteins involved in MVB formation, tetraspanins, membrane trafficking and fusion, and a number of cytosolic proteins. In addition to these generic components, molecules associated with particular cell types and/or pathological situations have also been identified in exosomes.
of 20-22 nucleotides that target the 3 untranslated region (UTR) of specific mRNAs to inhibit, in most cases, their translation. As a consequence of this, miRNAs can modify the phenotype and/or the physiology of the recipient cell, modulating cellular processes as relevant as proliferation, differentiation, and survival, among others (Cocucci and Meldolesi, 2015). The mechanisms that control the incorporation of the cargo into exosomes are currently under intense investigation (Villarroya-Beltri et al., 2014). Importantly, besides a ''constitutive'' array of proteins, lipids and RNAs, the content of exosomes varies according to the cellular source and to the physiological or pathological situation of the cell and its environment. Thereby, exosomes serve as interpreters of the cellular state where they came from. The role of exosomes as intercellular messengers has been studied extensively using cells of the immune system (Bobrie et al., 2011), where it has been demonstrated that they modulate antigen presentation and the immune response (Théry et al., 2009). Similarly, the role of exosomes in several cancer types has been well documented. For instance, glioblastoma-derived exosomes deliver genetic information to recipient endothelial cells, promoting tumor growth and invasion .
Once secreted, exosomes can interact with target cells to modify their function (Mittelbrunn and Sánchez-Madrid, 2012) and thus, they can act as local or paracrine messengers or communicators. Also, they can reach biological fluids, such as blood, CSF, urine, etc., and act as distant messengers ( Figure 3A). The targeting of exosomes to their recipient cells involves different mechanisms (Colombo et al., 2014;Mulcahy et al., 2014). In some cases, ligand/receptor interaction of EVs to target cells might be sufficient to activate intracellular pathways and induce physiological changes in target cells. In other cases, for instance when miRNAs contained in exosomes induce gene silencing in the recipient cell, the content of EVs must be transferred inside that cell. In those cases, fusion of exosomes with the PM or endocytosis followed by fusion of exosomes with the membrane of endocytic compartments must take place ( Figure 3A). Microenvironmental pH appears to be relevant for the fusion of exosomes with endosomal membranes (Parolini et al., 2009). Alternatively, a novel connexin 43-mediated mechanism to transfer information between exosomes and recipient cells has recently been described (Soares et al., 2015). According to this paper, the content of exosomes can be transferred to recipient cells by a gap junction-like communication, thus opening new possibilities about the mechanisms that might operate in specific environments or situations.

CAN EXOSOMES SERVE AS (PHYSIOLOGICAL OR PATHOLOGICAL) MESSENGERS BETWEEN CELLULAR COMPONENTS OF THE ADULT NEUROGENIC NICHE?
An efficient and well-regulated communication between cells is vital to ensure brain homeostasis and plasticity throughout life, particularly in the adult neurogenic niches. Thus, information transfer through exosomes (and other EVs) appears as a unique mechanism compared with other forms of intercellular communication. Even though the physiological and/or pathological role of locally-or distantly-generated exosomes in the adult neurogenic niches is currently virtually unknown, growing indirect evidence strongly suggest that exosomes might be one the major elements communicating and coordinating the function of the adult neurogenic niches. First, neural stem/progenitor cells and most of the cell types present in the CNS, including those cells that constitute and regulate the neurogenic niche, secrete and/or are target of exosomes and other EVs (Table 1). Furthermore, some of the biomolecules expressed (and sercreted) by niche cells have been reported to be present in exosomes under physiological or pathological conditions (Table 1)

Exosomes in Neuron-Neuron Communication
Classical inter-neuronal communication involves synaptic transmission, a dynamic and plastic process that is tightly regulated by neuronal activity (Regehr et al., 2009). Exosomemediated communication between pre and postsynaptic cells participates in synaptic plasticity, as it has been shown in the Drosophila neuromuscular junction (Korkut et al., 2013). Using cultures of mixed hippocampal cells with exosomes derived from the neuroblastoma cell line N2a and labeled with GFP-CD63 and GFP-TTC, it was found that they interact either with neurons, astrocytes or oligodendrocytes. On the other hand, exosomes released by cortical neurons upon synaptic activation interact with neurons but not with GFAP+ astrocytes. Furthermore, some exosomes co-localize with synaptophysin indicating that they bind to pre-synaptic sites (Chivet et al., 2014).

Exosomes in Neuron-Glia Communication
The communication between neurons and glia is important for brain physiology during both development and adulthood. The different glial cell types help to maintain neuronal activity. Oligodendrocytes protect axons with the myelin sheath and also provide trophic support to neurons (Nave and Trapp, 2008). To maintain these functions over time there is a constant communication between neurons and oligodendrocytes, but the mechanisms underlying this phenomenon are not well understood. Frühbeis et al. demonstrated that upon glutamate stimulation, oligodendrocytes secrete exosomes, which are endocytosed by neurons. Furthermore, exosomal cargoes improve neuronal metabolism and viability in situations of nutrient deprivation or oxidative stress exposure (Frühbeis et al., 2013b). It is also noteworthy that this work demonstrated that the internalization of exosomes by neurons occurs through a clathrin and dynamin-dependent mechanism, shedding light on the mechanisms that may be involved in exosome internalization. On the other hand, selective elimination of synaptic connections comprises the engulfment of neurites. In a recent study, it was shown that neuronderived exosomes stimulate microglial phagocytosis of neurites via upregulation of complement factors (Bahrini et al., 2015).

Exosomes in Glia-Glia Communication
The communication between glial cells through exosomes has been studied to a lesser extent. Exosomes secreted by oligodendrocytes are selectively internalized through macropinocytosis by microglia, both in vitro and in vivo (Fitzner et al., 2011). Remarkably, only those microglial cells that do not show antigen-presenting capacity endocytose exosomes, thus supporting the idea that different types of microglial cells co-exist and are differentially involved in immune functions.

Exosomes in the CSF as Volume Transmission Vehicles
It has been proposed that the CSF compartment plays an essential role in volume transmission within the CNS; thus, molecules or messengers secreted in one brain region may reach the CSF and exert their function in sites located far from its secretion site (Agnati and Fuxe, 2014;Borroto-Escuela et al., 2015). Given the close contact between the CSF and the interstitial fluid of several brain areas, including the SVZ, it is conceivable that exosomes originated in the brain parenchyma can be found in the CSF and vice versa. Actually, isolation of membrane vesicle-enriched fractions and further proteomic studies have demonstrated the presence of exosomes in the human CSF (Street et al., 2012;Grapp et al., 2013;Chiasserini et al., 2014). Furthermore, the exosome content of the CSF is supposed to reflect ongoing brain processes, and especially those related to plasticity, disease or repair. Proteins related to the onset or progression of some CNS diseases such as APP (Alzheimer's disease), PrPsc (prion disease), and α-synuclein (Parkinson's disease), among others, have been found in the exosomal fraction of CSF-samples (Pegtel et al., 2014). Exosomes in the CSF decrease with age while those derived from the embryonic CSF positively act on the stem cell niche (Street et al., 2012;Feliciano et al., 2014), revealing their influence on recipient cells. A clear demonstration of exosomal secretion into the CSF has been recently obtained in ChP epithelial cells. Using cell-culture assays, human CSF analyses and in vivo tracing experiments, the authors describe a novel pathway of exosome-mediated folate delivery into the CSF and subsequently, into the brain parenchyma (Grapp et al., 2013). Regulate NSC proliferation and differentiation (miR-let7b and miR-9) (Zhao et al., 2009 Favor exosome targeting and transfer of information to acceptor cells (Cx43) (Soares et al., 2015) Regulate the SGZ neurogenic niche (VEGF) (Kirby et al., 2015) Maintain protein homeostasis and regulates cell survival (Hsp70) (Takeuchi et al., 2015) Modulate gene expression in target cells via Stat1 activation (IFN-γ) (Cossetti et al., 2014) Mediate viral entry (TIM-4) (Sims et al., 2014) Modulates immune response (Cossetti et al., 2014) Autoimmunity (Kang et al., 2008) Neurophatological

Exosomes in the Blood to CNS Communication and as Therapeutic Vehicles
Exosomes constitute one of the most attractive vehicles to communicate peripheral organs with the CNS and vice versa.
The fact that blood-circulating exosomes may reach and be incorporated into different organs has stimulated scientists from diverse disciplines to explore the use of exosomes as therapeutic vehicles able to deliver specific drugs (Suntres et al., 2013). This widespread interest includes the use of exosomes as therapeutic agents in cancer (Pitt et al., 2014;Greening et al., 2015;Tran et al., 2015), and in infectious and allergic diseases (Admyre et al., 2008;Prado et al., 2008;Hosseini et al., 2013), to give just a few examples. Exosomes are considered attractive vehicles of blood to CNS communication due to (i) their stability (their cargo is protected from RNAses and proteases); (ii) their lack of immunogenicity (when derived from the same patient); (iii) the possibility of adding surface proteins or antibodies to target specific cell types; (iv) the possibility of loading a specific molecular cargo with therapeutic actions; and (v) importantly, their capacity to cross the BBB (Aryani and Denecke, 2014;Ridder et al., 2014;György et al., 2015;Kawikova and Askenase, 2015). siRNAs, for example, have been successfully targeted to specific brain regions in mice following systemic injection of siRNA-electroporated exosomes (Alvarez-Erviti et al., 2011c;El-Andaloussi et al., 2012). This strategy has been reported to be effective as a way to decrease α-synuclein aggregates in wild type as well as in transgenic mice expressing the phospho-mimic S129D α-synuclein, which is prone to aggregation (Cooper et al., 2014). Exosomes have also been used to deliver curcumin and JSI124 (activator of transcription 3 inhibitor) to brain microglia of mice via an intranasal route, protecting from inflammation and delaying tumor growth (Zhuang et al., 2011). These examples emphasize the potential of exosomes, not only as therapeutic vehicles, but also as physiological and pathological messengers between peripheral organs and the CNS. Even though the mechanisms by which peripheral exosomes have access to the brain tissue are unknown, the fact that adult NSCs reside in vascular niches, leads to the interesting proposal that bloodborne exosomes may influence adult neurogenic niches.

Exosomes as Modulators of Diverse Stem Cell Niches
Cancer stem cells (CSCs) or cancer initiating cells (CICs) are tumor cells that have properties of self-renewal, clonal tumor initiation, and metastatic potential (Zhang et al., 2015b). As other stem cells, CSCs reside in distinct regions within the tumor called niches, which protect CSCs from immune responses and preserve their phenotypic plasticity (Plaks et al., 2015). Furthermore, the niche is believed to play a pivotal role in the resistance of CSCs to some cancer therapies (Kuhlmann et al., 2015;Plaks et al., 2015). It is known that exosomes released locally from tumor cells are able to modify the niche, promoting angiogenesis and tumor cell proliferation (Tickner et al., 2014). In addition, cancerderived exosomes can travel to sites located outside the tumor to induce cellular changes associated with the promotion of a ''pre-metastatic'' niche, a special microenvironment that is able to receive and harbor cancer cells, thus favoring metastasis. This has been demonstrated in the case of melanoma metastasis in the bone marrow (Peinado et al., 2012) and liver metastasis of pancreatic ductal adenocarcinoma (Costa-Silva et al., 2015). Even though the mechanisms by which exosomes modulate primary tumor and pre-metastatic niches are not fully understood, it has been shown that isolated exosomes from body fluids of cancer patients (blood, ascites fluid and urine, CSF) contain several growth factors and cytokines able to modulate the environment of the metastatic niche (Ung et al., 2014). On the other hand, non-tumoral mesenchymal stem cells (MSCs) are extensively used in different cell therapy-based clinical trials today. It is known that functional improvement with MSCs therapies is not mainly due to cell engraftment or differentiation at the site of injury but they exert their effects through their secreted products, including exosomes (Lai et al., 2015). Exosomes harvested from the conditioned media of MSCs cultures increase angiogenesis and neurogenesis, and promote functional recovery in animal models of stroke and traumatic brain injury (Xin et al., 2013a,b;Zhang et al., 2015c). Thus, in therapies for brain disorders, it has been proposed that exosomes derived from MSCs function as an extension of MSCs, and like their cell-source, exosomes can target biological processes that stimulate functional repair of the damaged nervous system (Lai et al., 2015). However, it is thought that similar exosomes isolated from MSCs of different origins (i.e., bone marrow, menstrual, chorion or umbilical cord) might differentially affect target cells, possibly due to a differential molecular cargo (Lopez-Verrilli and Court, 2013). In this context, albeit a direct participation of exosomes in the adult neurogenic niches has not yet been addressed, it is conceivable that exosomes derived from the different cell types of the neurogenic niche as well as the heterogeneous exosome mixture that may reach the niche via CSF-volume transmission or through the vasculature, affect the neurogenic process in a differential and highly specific manner. Given the relevance of these actions to a large array of diseases of the nervous system, the cellular and molecular mechanisms involved in the regulatory functions of exosomes in the neurogenic niche are an attractive field for future investigations.

MOLECULAR COMPONENTS OF EXOSOMES WITH A POTENTIAL ROLE IN THE REGULATION OF THE NEUROGENIC NICHE MicroRNAs
Several steps of adult neurogenesis are mediated or regulated by miRNAs. For example, miR-let7b and miR-9 regulate proliferation and differentiation of NSCs (Zhao et al., 2009. Similarly, miR-34a regulates NSCs differentiation, neuroblast migration and neuron maturation (Mollinari et al., 2015). miR-124a is a key determinant of neuronal fate of SVZ NSCs by targeting Sox9 (Cheng et al., 2009;Akerblom et al., 2012) or by targeting the JAG-Notch signaling pathway (Liu et al., 2011). On the other hand, miR-128 overexpression reduces the levels of doublecortin (Dcx) in differentiating NPCs, indicating that miR-128 can target and potentially take part in the regulation of Dcx levels in adult neurogenesis (Cernilogar et al., 2015). Furthermore, it has been recently shown that miR-124, miR-128 and miR-137, can act cooperatively and synergistically to promote neuronal differentiation of NSCs by targeting overlapping gene sets containing a highly interconnected transcription factor network (Santos et al., 2015). miRNAs are also involved in glial cell proliferation/differentiation and angiogenesis, phenomena closely related and associated with adult neurogenesis. For example, miR-125b is involved in glial cell proliferation under physiological and pathological conditions (Pogue et al., 2010). On the other hand, miR-126 and miR-214 induce angiogenesis (Sheldon et al., 2010;van Balkom et al., 2013van Balkom et al., , 2015Sharghi-Namini et al., 2014), while miR-296 increase levels of pro-angiogenic receptors . Several CNS disorders associated with defective neurogenesis has been linked to miRNAs function. For example, dysregulation of miR-124 is associated with neurodegenerative and stress-related disorders (Sun et al., 2015). Similarly, miR-155 is essential for inflammation-induced hippocampal neurogenic dysfunction via microglial activation (Woodbury et al., 2015).
Despite their relevant and determinant role on the neurogenic process, the precise target cells and mechanisms by which miRNAs are transferred to target cells in the neurogenic niche are virtually unknown. However, all the miRNAs mentioned above have been found in exosomes from different cellular origins (Table 1). Furthermore, most of them are expressed by different niche cells; thus, it is conceivable that some of these miRNA are transported within exosomes to exert their function on NSCs/NPCs and regulate the neurogenic process. In addition, a recent systemic characterization of exosomal RNA profiles in human plasma samples by RNA sequencing analyses showed that miRNAs were the most abundant (Huang et al., 2013). Interestingly, the same study showed that five (miR-128, miR-124, miR-125b, miR-9, and miR-let7b) out of the twenty most abundant exosomal miRNAs are involved in the neurogenic/angiogenic process. Thus, these results highlight not only the fact the neurogenesis-modulating miRNAs are incorporated into exosomes, but also stress the possibility of exosome-mediated communication between the systemic circulation and the CNS.

Proteins and Signaling Peptides
Some of the biomolecules that have already been functionally characterized as modulators of adult neurogenesis, have also been described as components of exosomes. For example, TGF-β, a negative regulator of the adult neurogenic niche, has been documented to be present in exosomes of diverse cellular origins. As such, TGF-β-carrying exosomes circulate in the blood stream under diverse pathological conditions ranging from renal to pregnancy-related diseases and in consequence, might indirectly affect neurogenesis if crossing the BBB (Szajnik et al., 2013;Hong et al., 2014;Tan et al., 2014;Torreggiani et al., 2014;Raimondo et al., 2015;Solé et al., 2015). In addition to the protein, the mRNA coding for TGF-β has been found in exosomes from glioblastoma multiforme patients (Muller et al., 2015). On the other hand, positive regulators of adult neurogenesis such as Ephrin-B2 or components able to activate EGFR signaling have also been found in exosomes (Mathivanan et al., 2010;Higginbotham et al., 2011). Similarly, VEGF has been found in exosomes derived from several cell types (Thompson et al., 2013;Ekström et al., 2014;Torreggiani et al., 2014). Interestingly, it has been recently shown that adult hippocampal NSCs/NPCs secrete large amounts of VEGF in vitro and in vivo and this self-derived VEGF is functionally relevant for maintaining the neurogenic niche (Kirby et al., 2015). Several other signaling peptides known to determine cell fate in the neurogenic niche and described in the present work, such as PEDF, IGFBP6, EGF, FGF-2, Hedgehog, Notch, as well as proteins of the Wnt signaling pathway, just to mention a few, have been found in the exosomal fractions from different cell types and conditions (Graner et al., 2009;Nazarenko et al., 2010;Lai et al., 2011;Hajrasouliha et al., 2013;Wendler et al., 2013). Remarkably, most of the studies describing the presence of these proteins in exosomes correspond to proteomic analyses. In this regard, several intriguing issues remain to be addressed. The mechanisms involved in the delivery of several growth factors and signaling peptides to MVBs and their incorporation into ILVs are still speculative. Moreover, the molecular machinery responsible of transferring exosomal cargo into target cells are unknown. Further studies conducted to elucidate these molecular mechanisms will give clues about how adult neurogenesis is regulated not only under physiological conditions but also under certain CNS disorders.

CNS DISORDERS ASSOCIATED TO IMPAIRED ADULT NEUROGENESIS: POTENTIAL ROLE OF EXOSOMES IN THEIR PATHOGENESIS AND AS BIOMARKERS
Several neurodegenerative disorders have been associated to defects in the adult neurogenic process in the DG and/or subventricular zone/OB system (Steiner et al., 2006;Shruster et al., 2010;Mu and Gage, 2011;Winner et al., 2011;Regensburger et al., 2014;Foltynie, 2015;He and Nakayama, 2015;Le Grand et al., 2015;Winner and Winkler, 2015). On the other hand, significant progress has been done to unravel the function and regulation of adult neurogenesis in psychiatric diseases (Eisch et al., 2008;Jun et al., 2012). Depression is associated to a reduction in SGZ neural progenitor proliferation in the DG (Gould et al., 1998;Jacobs et al., 2000). Similarly, experimentally-induced inhibition of neurogenesis favors depressive-like behaviors in animal models (Wang et al., 2015;Xiang et al., 2015). On the other hand, different therapies that relieve depressive symptoms, such as antidepressant drug treatments (Malberg et al., 2000;Santarelli et al., 2003) or physical exercise (van Praag et al., 1999a;Lugert et al., 2010), increase SGZ neurogenesis. However, the mechanisms that stimulate adult hippocampal neurogenesis in those treatments are currently not clear (Jun et al., 2012).
Even though we are yet to obtain a conclusive causal relationship between adult neurogenesis and neurodegenerative or psychiatric diseases, growing evidence reveals how these disorders may proceed through impairment in the regulation of adult neurogenic niches. Thereby, despite their particular genetic or environmental origin, defects in adult neurogenesis appears as common hallmark functionally associated to the pathogenesis of different CNS diseases. Recent studies emphasize a putative role of exosomes in the pathogenesis of neurodegenerative and psychiatric disorders. Furthermore, considering that (i) exosomes may condition the microenvironment of a particular region such as the neurogenic niche; (ii) the content of exosomes in a particular cell type may change in different physiological and pathological conditions; and (iii) that brain parenchyma-derived exosomes may have access to the CSF and the peripheral blood circulation, neurogenic niche-derived exosomes may represent not only powerful regulators of adult neurogenesis, but also attractive therapeutic targets and useful biomarkers for different CNS disorders.

Exosomes in Neurodegenerative Disorders
In neurodegenerative disorders such as Alzheimer's disease (Rajendran et al., 2006;Saman et al., 2012), Parkinson's disease (Emmanouilidou et al., 2010;Alvarez-Erviti et al., 2011a,b) and Prion diseases (Fevrier et al., 2004), the content of pathological forms of the toxic proteins associated to exosomes is increased and as such, it has been proposed that exosomes may favor the amplification and spread of protein misfolding diseases (Bellingham et al., 2012b;Danzer et al., 2012;Grad et al., 2014). In fact, when the production of exosomes was inhibited in a mouse model of Alzheimer's disease by decreasing the synthesis of ceramide, a lipid enriched in exosomes, total amyloid as well as plaque levels were reduced (Dinkins et al., 2014). In brain cells, the abnormal protein aggregates present in neurodegenerative disorders activate the proteasome and autophagy pathways, tending to restore proteostasis (protein homeostasis; Hetz and Mollereau, 2014). A possible explanation of exosomal loading and secretion of cytotoxic proteins is that the overload of misfolded proteins may saturate the mentioned cytoprotective pathways, leading to their elimination via exosomes, although the mechanistic links of these processes are yet unknown . Interestingly, exosomes might also act by sequestering toxic proteins (Kalani et al., 2014). In Alzheimer's Disease, in which exosomes are loaded with amyloid-β (Aβ) peptides and with molecules involved in its synthesis, degradation and aggregation, exogenous exosomes expressing surface proteins such as the cellular prion protein (PrPc), a receptor for Aβ, can sequester Aβ and counteract its negative effects on plasticity .

Exosomes in Psychiatric Disorders
Almost nothing is known about changes in exosome content or release and their action on recipient cells in psychiatric disorders, such as schizophrenia, major depressive disorder, bipolar disorder or anxiety disorders, among others (Tsilioni et al., 2014). Interestingly, defects in the adult neurogenic process have been associated to the pathogenesis and/or progression of most of these disorders. In schizophrenia and bipolar disorders, specific exosome-related miRNAs (miR-497 and miR-29c, respectively) were upregulated in the prefrontal cortex of post-mortem brains (Banigan et al., 2013). We have shown that treatment with the antidepressant drug fluoxetine upregulates the content of the forebrain astrocyte-derived enzyme Aldolase C in CSF exosomes. The content of this enzyme is further upregulated in exosomes after chronic restraint stress, but not after stress induced by complete immobilization (Sandoval et al., 2013;Ampuero et al., 2015). A better knowledge of molecular changes associated to brain-derived exosomes in psychiatric disorders will allow a better comprehension of the neurobiological features of these complex diseases and in turn, would lead to the proposition of new treatment strategies.

Exosomes as Potential Biomarkers of CNS Disorders
One of the yet unmet goals in CNS diseases, especially in the psychiatric sphere, is the establishment of biological markers, especially those obtained by non-invasive strategies, which could be used as diagnostic tools or to monitor disease progression, treatment effects and prognosis. Thus, the miRNA and protein cargo of exosomes obtained from peripheral body fluids, such as plasma or urine, constitute remarkable candidates as biomarkers (Cheng et al., 2014;Zhang et al., 2015a). With the exception of an intracranial cancer type, glioblastoma multiforme Shao et al., 2012), it has not yet been possible to show in a convincing way that exosomes produced by neurons or glial cells might reach the blood circulation. Serum exosomes from glioblastoma multiforme patients carry tumor specific epidermal growth factor receptor vIII (EGFRvIII), and transforming growth factor beta 1 (TGF-β1; Skog et al., 2008;Graner et al., 2009). The proposed pathways are a defective BBB (Sáenz-Cuesta et al., 2014), a CSF-blood pathway or a transport process by transcytosis across endothelial cells in an intact BBB, as it has recently been suggested (Haqqani et al., 2013).
The fact that neurodegenerative diseases are associated with increases in plasma exosomes containing misfolded, pathological forms of proteins strongly suggest a central origin of them. Indeed, exosomes can carry proteins that serve as common biomarkers of a disease. A neuroblastoma cell line (SH-SY5Y) expressing α-synuclein release exosomes that contain this protein and are capable of transporting α-synuclein to SH-SY5Y cells that do not express it (Alvarez-Erviti et al., 2011a).
In vivo experiments have shown that a small proportion of radioactively labeled α-synuclein delivered to the CSF can be recovered in plasma exosomes (Shi et al., 2014) pointing to a central origin of the protein, although its cellular origin remains obscure. Neurons infected with prion protein in its cellular (PrP c ) as well as with its pathogenic form (PrP sc ) release exosomes containing prion proteins as well as a specific miRNA signature (Fevrier et al., 2004;Vella et al., 2007;Bellingham et al., 2012a). In intracerebrally infected animals, exosomes carrying PrP sc , accounting for about 20% of the plasma infectivity, could be harvested from plasma samples (Properzi et al., 2015). In this study, PrP sc was identified by Western blots with the caution of using sucrose gradient-purified exosomes to avoid the presence of bloodderived immunoglobulin contaminations that would obscure the usefulness of protein exosome markers in the molecular weight range of the IgG and IgM light and heavy chains (Properzi et al., 2015).
To identify EVs of neuronal origin, affinity purification using antibodies against neuronal membrane proteins, such as neural cell adhesion molecule-1 (NCAM-1) or neural cell adhesion molecule L1 (L1CAM), have been used. With this experimental strategy, the group of Kapogiannis has been able to detect plasma or serum exosomes with increased content of Aβ1-42, and of phosphorylated forms of tau and type 1 insulin receptor substrate (IRS-1) specifically associated to patients suffering Alzheimer's Disease. Interestingly, these findings are proposed as useful predictive tools up to 10 years before the clinical onset of the disease (Fiandaca et al., 2015;Kapogiannis et al., 2015). In summary, the molecular content, i.e., miRNAs or proteins, in exosomes are emerging as strong candidates for providing CNS disease-specific biomarkers. The consistency of the results depends on the isolation method and thus, the purity of the fraction under analysis (exosomes vs. microparticles or microvesicles), or contamination with extravesicular bloodborne molecules while their origin in neuronal or glial cells is awaiting an indisputable proof. Furthermore, despite their purity, there are other shortcomings that need be to be addressed when attempting to use exosomes as biomarkers: (i) the pattern of exosomal RNA may change according to the extraction methods, due to differential susceptibility to exosomal lysis according to their membrane composition (Eldh et al., 2012;Van Aelst and Heymans, 2013); (ii) problems when trying to replicate previously published microarray analysis have been reported, mainly due to journals not enforcing more strict guidelines to interpret and report microarray data (Shields, 2006;Ioannidis et al., 2009); (iii) a further source of confusion is the possibility that medications taken by patients may alter the composition of exosomes, thus adding another level of complexity in the analysis and interpretation of the data (Aryani and Denecke, 2014).
FIGURE 4 | Exosomes as regulators of adult neurogenesis. The NSC-neuron lineage is exposed to a complex mix of exosomes within the neurogenic niche. Exosomes secreted locally by different niche cells can influence the physiology of other niche cells and the progression of different neurogenic stages. It is well demonstrated that neurons, astrocytes, microglia and endothelial cells secrete exosomes. NSCs/NPCs are also able to secrete exosomes (not depicted). Exosomes release by ependymal cells and pericytes has not been reported up to now (?). Additionally, exosomes originated in cells located far from the neurogenic niches can influence its nature. Exosomes derived from cells located in other brain regions can reach neurogenic niches through innervation of the niche or via CSF-mediated volume transmission. Indeed, besides being a source of soluble molecules for the SVZ neurogenic niche, the presence of exosomes in the CSF has been demonstrated in several mammalian species including humans. Furthermore, choroid plexus epithelial cells secrete exosomes into the CSF. Interestingly, exosomes produced in cells and tissues outside the central nervous system (CNS; peripheral tissues) can potentially reach neurogenic niches either directly, through the vasculature (blood) of the niche, or indirectly, through the choroid plexus. Conversely, exosomes originated in the neurogenic niche might have access to the CSF and to the peripheral blood circulation. The ability of exosomes to cross-communicate the CNS (neurogenic niches) with peripheral tissues highlights their potential role as physiological/pathological mediators of different CNS disorders (explanation for CNS-peripheral tissues co-morbidities, for example) and as biomarkers (CSF/blood samples). BBB, blood-brain barrier; BCB, blood-CSF barrier (choroid plexus epithelial cells); CBI, CSF-brain interface.

CONCLUSION AND FUTURE PERSPECTIVES
In this review, we focus on the potential role of EVs, and in particular, of exosomes in the regulation of the neurogenic niche (Figure 4). Exosomes, as messengers able to modulate the physiology of the niche, might originate from cells residing within the niche or from distant cells/tissues, thus having access to the neurogenic niche through the vasculature (bloodcirculating exosomes) or by volume transmission via the CSF. The possible effects of molecular components already known to be present within exosomes on adult neurogenic process are also addressed. This scenario opens the possibility of a novel form of communication between niche cells and regulation of adult neurogenesis, able to both, regulate locally the extent of neurogenesis, and sense and integrate physiological conditions and pathological disturbances in diverse body systems.
On the other hand, exosomes originated in the CNS and in the neurogenic niche (i) can vary according to the circumstances, and (ii) might reach the peripheral blood circulation, thus linking or communicating directly the physiological or pathological status of the CNS to peripheral organs or tissues. This could be relevant (1) to understand the high prevalence of comorbidity of pathologies associated to impaired neurogenesis and peripheral disorders, such as major depression and diabetes or inflammatory diseases (Kessler et al., 2003;Empana et al., 2005;Evans et al., 2005;Katon et al., 2008;Katon, 2008;Bonaz and Bernstein, 2013;Filipovic and Filipovic, 2014), and (2) to use blood-circulating CNS-derived exosomes as biomarkers of brain disorders.
The contribution of specific cell-and tissue-derived exosomes on adult neurogenesis should be further investigated with the use of proper animal models in which exosomes should be labeled with the use of molecular biology techniques, and later should be validated in health and disease with the use of a panel of biomarkers able to define specific exosome populations. We envisage that, in the near future, many of this work will be addressed by a growing community of researchers interested in the role of exosomes in disease-related processes that, among others, affect the neurogenic niche in a yet unsuspected manner.

AUTHOR CONTRIBUTIONS
All the authors have contributed substantially to the writing and revising of the manuscript. LFB and UW have participated in the conception and design of the work. LFB have designed the figures. RIM, ZDV and LFB have designed and completed the table. MAC, RIM, PVB, ZDV, CAL, P-ET have participated in writing, drafting and revising of different sections of the manuscript. All the authors have approved the final version of the manuscript.