AUTHOR=SpĆ¼hler Isabelle A. , Conley Gaurasundar M. , Scheffold Frank , Sprecher Simon G. TITLE=Super Resolution Imaging of Genetically Labeled Synapses in Drosophila Brain Tissue JOURNAL=Frontiers in Cellular Neuroscience VOLUME=10 YEAR=2016 URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2016.00142 DOI=10.3389/fncel.2016.00142 ISSN=1662-5102 ABSTRACT=
Understanding synaptic connectivity and plasticity within brain circuits and their relationship to learning and behavior is a fundamental quest in neuroscience. Visualizing the fine details of synapses using optical microscopy remains however a major technical challenge. Super resolution microscopy opens the possibility to reveal molecular features of synapses beyond the diffraction limit. With direct stochastic optical reconstruction microscopy,