Original Research ARTICLE
LncRNA MALAT1 promotes glioma tumorigenesis by sponging miR-129
- 1Affiliated Hospital of Hebei University of Engineering, China
Purpose: We aimed to explore the regulatory mechanisms of lncRNA MALAT1 on miR-129 and investigate the effect of MALAT1 on the proliferation of glioma stem cells and glioma tumorigenesis.
Methods: Differentially expressed lncRNAs in glioma cells and glioma stem cells were screened out with microarray analysis. The targeting relationship between miR-129 and MALAT1 or SOX2 was validated by dual-luciferase reporter assay. The expressions of MALAT1, miR-129 and SOX2 mRNA in both glioma cells and glioma stem cells were examined by qRT-PCR assay. The impact of MALAT1 and miR-129 on glioma stem cell proliferation was observed by CCK-8 assay, EdU assay and sphere formation assay. The protein expression of SOX2 was determined by western blot. The effects of MALAT1 and miR-129 on glioma tumor growth were further confirmed using xenograft mouse model.
Results: The mRNA expression of MALAT1 was significantly upregulated in glioma stem cells compared with glioma cells, while the expression of miR-129 was significantly downregulated in glioma stem cells. MALAT1 promoted glioma stem cell reproduction via regulation of miR-129. Meanwhile, miR-129 directly targeted at SOX2 and overexpression of miR-129 suppressed cell viability and proliferation of glioma stem cells by suppressing SOX2 expression. The down-regulation of MALAT1 and miR-129 overexpression suppressed glioma tumor growth in vivo.
Conclusion: MALAT1 enhanced glioma stem cell viability and proliferation abilities and promoted glioma tumorigenesis through sponging miR-129 and facilitating SOX2.
Keywords: lnRNA MALAT1;, miR-129;, SOX2;, gliom;, tumorigenesis;, proliferation
Received: 28 Sep 2017;
Accepted: 20 Dec 2017.
Edited by:James F. Curtin, Dublin Institute of Technology, Ireland
Reviewed by:Alessandro Laganà, Icahn School of Medicine at Mount Sinai, United States
Cristiana Tanase, Victor Babes National Institute of Pathology, Romania
Copyright: © 2017 Wang, Li, Ma and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Ji-Min Zhang, Affiliated Hospital of Hebei University of Engineering, Handan, China, email@example.com