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Review ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Neurosci. | doi: 10.3389/fncel.2019.00381

Genes and mechanisms involved in the generation and amplification of basal radial glial cells

  • 1INSERM U839 Institut du Fer à Moulin, France
  • 2Institut National de la Santé et de la Recherche Médicale (INSERM), France
  • 3University Medical Centre Mannheim, University of Heidelberg, Germany
  • 4Hector Institute for Translational Brain Research, Central Institute of Mental Health (ZI), Germany
  • 5German Cancer Research Center (DKFZ), Germany

The development of the cerebral cortex relies on different types of progenitor cell. Among them, the recently described basal radial glial cell (bRG) is suggested to be of critical importance for the development of the brain in gyrencephalic species. These cells are highly numerous in primate and ferret brains, compared to lissencephalic species such as the mouse in which they are few in number. Their somata are located in basal subventricular zones in gyrencephalic brains and they generally possess a basal process extending to the pial surface. They may also have an apical process directed towards the ventricular surface, similar to apical radial glial cells (aRGs) from which they are derived, and whose somata are found more apically in the ventricular zone. bRGs share similarities with aRGs in terms of gene expression (SOX2, PAX6, NESTIN), whilst also expressing a range of more specific genes (such as HOPX). In primate brains, bRGs can divide multiple times, self-renewing and/or generating intermediate progenitors and neurons. They display a highly specific cytokinesis behavior termed mitotic somal translocation. We focus here on recently identified molecular mechanisms associated with the generation and amplification of bRGs, including bRG-like cells in the rodent. These include signaling pathways such as the FGF-MAPK cascade, SHH, PTEN/AKT, PDGF pathways, and proteins such as INSM, GPSM2, ASPM, TRNP1, ARHGAP11B, PAX6 and HIF1α. A number of these proteins were identified through transcriptome comparisons in human aRGs versus bRGs, and validated by modifying their activities or expression levels in the mouse. This latter experiment often revealed enhanced bRG-like cell production, even in some cases generating folds (gyri) on the surface of the mouse cortex. We compare the features of the identified cells and methods used to characterize them in each model. These important data converge to indicate pathways essential for the production and expansion of bRGs, which may help us understand cortical development in health and disease.

Keywords: Cortical development, neural progenitor cells, radial glia, spindle orientation, Adhesion, signaling pathway, Cell Division

Received: 11 Apr 2019; Accepted: 05 Aug 2019.

Edited by:

Carlos Cardoso, INSERM U901 Institut de Neurobiologie de la Méditerranée, France

Reviewed by:

JAMES Y. LI, University of Connecticut Health Center, United States
Alejandro Lopez Tobon, Istituto Europeo di Oncologia s.r.l., Italy
Ginam Cho, Brigham and Women's Hospital, Harvard Medical School, United States  

Copyright: © 2019 Penisson, Ladewig, Belvindrah and FRANCIS. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Fiona FRANCIS, INSERM U839 Institut du Fer à Moulin, Paris, France, fiona.francis@inserm.fr