Expression of the ACE2 Virus Entry Protein in the Nervus Terminalis Reveals the Potential for an Alternative Route to Brain Infection in COVID-19

Previous studies suggested that the SARS-CoV-2 virus may gain access to the brain by using a route along the olfactory nerve. However, there is a general consensus that the obligatory virus entry receptor, angiotensin converting enzyme 2 (ACE2), is not expressed in olfactory receptor neurons, and the timing of arrival of the virus in brain targets is inconsistent with a neuronal transfer along olfactory projections. We determined whether nervus terminalis neurons and their peripheral and central projections should be considered as a potential alternative route from the nose to the brain. Nervus terminalis neurons in postnatal mice were double-labeled with antibodies against ACE2 and two nervus terminalis markers, gonadotropin-releasing hormone (GnRH) and choline acetyltransferase (CHAT). We show that a small fraction of CHAT-labeled nervus terminalis neurons, and the large majority of GnRH-labeled nervus terminalis neurons with cell bodies in the region between the olfactory epithelium and the olfactory bulb express ACE2 and cathepsins B and L. Nervus terminalis neurons therefore may provide a direct route for the virus from the nasal epithelium, possibly via innervation of Bowman’s glands, to brain targets, including the telencephalon and diencephalon. This possibility needs to be examined in suitable animal models and in human tissues.

However, the route along the olfactory nerve from the nose to the brain is controversial for SARS-CoV-2, primarily for two reasons: (1) the olfactory receptor neurons do not express the obligatory virus entry receptor, angiotensin-converting enzyme 2 (ACE2), or expression is restricted to a very small subset of these neurons Cooper et al., 2020;Brechbühl et al., 2021;Butowt et al., 2021). Because sustentacular cells tightly enwrap olfactory receptor neurons (Liang, 2020), these ACE2-expressing support cells can easily be mistaken for olfactory receptor neurons, resulting in false positive identification.
(2) The timeline of appearance of SARS-CoV-2 in the brain is inconsistent with a "neuron-hopping" mode: infection of third-order olfactory targets should occur with a significant delay after infection of the olfactory epithelium, as has been reported for other neuro-invasive viruses (Barnett et al., 1995), but instead the hypothalamus and brainstem are reported to be infected as early as, or even earlier than, the olfactory bulb (de Melo et al., 2021;Zheng et al., 2021), and SARS-CoV-2 may even skip the olfactory nerve and olfactory bulb on its way to brain infection (Winkler et al., 2020;Zhou et al., 2020;Carossino et al., 2021). These findings have raised doubt about the notion that the olfactory nerve serves as a major conduit for brain infection in COVID-19 (Butowt et al., 2021).
With few exceptions (Briguglio et al., 2020;Butowt et al., 2021), studies suggesting an olfactory route for SARS-CoV-2 to achieve brain infection fail to consider the potential for an alternative route from the nose to the brain, the route via the nervus terminalis. Many peripheral processes of the nervus terminalis innervate the olfactory epithelium, the blood vessels below this epithelium, as well as cells in Bowman's glands (Larsell, 1950), and the central processes of some of these neurons extend to various targets in the forebrain as far caudal as the hypothalamus (Pearson, 1941;Larsell, 1950;Schwanzel-Fukuda et al., 1987;Demski, 1993;von Bartheld, 2004). Some of the nervus terminalis neurons are in direct contact with spaces containing cerebrospinal fluid (CSF) in the region of the olfactory nerve and bulb (Jennes, 1987). About 30-40% of the neurons of the nervus terminalis express gonadotropin-releasing hormone (GnRH), and some of these neurons may release GnRH into blood vessels below the olfactory epithelium (Jennes, 1987;Schwanzel-Fukuda et al., 1987), while other neuronal populations of the nervus terminalis system are thought to regulate blood flow and blood pressure in the nose and forebrain (Larsell, 1918;Oelschläger et al., 1987;Ridgway et al., 1987). These properties make the nervus terminalis a strong candidate for expression of ACE2, which is known to regulate blood flow and blood pressure in many tissues (Tikellis and Thomas, 2012). Expression of ACE2 in the nervus terminalis would suggest that this cranial nerve is a plausible alternative to the olfactory nerve for the SARS-CoV-2 virus to gain access to the brain. However, it has not been previously examined and reported whether nervus terminalis neurons express the obligatory viral entry receptor, ACE2, and any other virus entry proteases such as TMPRSS2 and cathepsins B and L. We have therefore examined whether these entry proteins are expressed in nervus terminalis neurons in an animal model, the postnatal mouse.

Animals and Tissue Processing
A total of eight wildtype C57BL/6J mice (Jackson Laboratory) at age 3-4 weeks old were used to obtain tissues for experiments. Mice were housed with a 12/12 h light/dark cycle and given access to water and food ad libitum. All animal experiments were approved by the local ethics committee for animal research at Bydgoszcz (Poland). Immediately after cervical dislocation, the mice were exsanguinated and tissues were dissected. Olfactory epithelium and brain were frozen at −80 • C for storage and further usage, or fixed for 3 h at 4 • C in 4% (w/v) freshly prepared paraformaldehyde in phosphate-buffered saline (PBS, pH 7.5), and then incubated in 25% (w/v) sucrose/PBS at 4 • C for 16-24 h, frozen in Tissue-Tek O.C.T. (Sakura Finetek), and cryosectioned at 10-12 µm using a Leica CM1850 cryostat.

ACE2 −/− Knockout (ACE2 KO) Control
To verify the specificity of the ACE2 antibody, an ACE2 knock-out (KO) mouse line was obtained from Taconic (strain #18180). Two male homozygous ACE2 KO mice at age 3 weeks old were processed and immunolabeled as described below for wildtype mice. Genotyping was performed according to the manufacturer's suggested PCR protocol. Lack of an ACE2 protein band was confirmed by using Western blots as described previously . In brief, tissue was homogenized on ice in N-Per Total Protein Extraction reagent (Thermo Scientific) with addition of protease and phosphatase inhibitor cocktails (Sigma-Aldrich). Homogenates were centrifuged for 30 min at 20,000 g at 4 • C and supernatants were collected. Protein content was measured by the BCA method (Thermo Scientific). Equal amounts of total proteins were mixed with 4× Laemmli sample buffer and boiled for 10 min at 80 • C. Protein extracts were separated on SDS-PAGE 7.5% gels and mini-protean III apparatus. GAPDH was used as positive control and to verify equal loading. Proteins were blotted to nitrocellulose membranes using standard Tris-glycine wet method. Membranes were blocked with 5% dry milk (Bio-Rad), incubated with goat polyclonal anti-ACE2 (R&D Systems AF3437) at 1/1,000 or rabbit polyclonal anti-GAPDH (Protein-Tech, #10494-1-AP) at 1/5,000 dilution overnight at 4 • C, washed several times in TBST buffer (Tris-buffered saline with 0.05% Tween 20, pH 8.0) and incubated 60 min with secondary antibody, anti-goat-HRP (Protein-Tech). Signal was detected using Clarity Max chemiluminescence substrate (Bio-Rad). For confirmation, blots were stripped and re-probed with an additional rabbit monoclonal anti-ACE2 antibody (Abclonal, #A4612). Blots were prepared in three separate experiments with comparable results.

ACE2 Immunocytochemistry and Co-localization Analysis
For double immunofluorescence labeling, antigen retrieval procedure was performed on frozen sections cut at 10-12 µm. Sections were incubated overnight with a mixture of primary goat anti-ACE2 at 1/500 dilution (R&D Systems, #AF3437) and rabbit anti-GnRH (gonadotropin releasing hormone) at 4 • C. On the next day, sections were washed five times in PBST (PBS with 0.05% Triton X-100) and incubated with a mixture of secondary anti-rabbit-AF488 antibody and anti-goat-AF594 at 1/500 dilution for 60 min at room temperature. Next, sections were stained for 5 min at room temperature in Hoechst 33258 (Sigma-Aldrich) to visualize cell nuclei, and sections were then embedded in aqueous antifade medium (Vector laboratories). Alternating cryosections were incubated with rabbit polyclonal anti-CHAT (choline acetyltransferase) instead of rabbit anti-GnRH antibody in the double staining primary antibody mixture. Occasionally, sections were incubated with anti-OMP (olfactory marker protein) at 1/500 dilution in PBST, following the same protocol. After immunocytochemical reactions, sections were analyzed on a Nikon Eclipse 80i microscope and images were taken using a Nikon DP80 camera. Microscopic images were processed using cellSens Dimension 1.13 software (Olympus). Antibodies and vendors are listed in Supplementary Table 1. To compare the signal intensity between nervus terminalis neurons and cells known to express ACE2 and to internalize SARS-CoV-2, the ACE2 fluorescent signal was compared by measuring the optical density of the signal in gray scale (8-bit maps) in ACE2expressing nervus terminalis neurons and in sustentacular cells of the dorsal olfactory epithelium, using cellSens Dimension 1.13 software (Olympus). Intensity values were defined by regions of interest and a quantitative immunofluorescence score was calculated by comparing the target mean gray intensity for 15 sustentacular cells and 12 GnRH-positive nervus terminalis neurons.

Cell Counting and Statistical Analysis
For counting double-labeled neurons, five male wildtype mice at age 3-4 weeks old were used. Approximately every third coronal cryosection (10-12 µm thickness) was stained as described above, and positive neurons were counted in tissue sections under a fluorescent microscope as indicated in Figure 1 (the medial region from the posterior olfactory epithelium to the caudal end of the olfactory bulb). For each animal, the percentage of double labeled GnRH + /ACE2 + neurons was calculated in relation to the total number of GnRH-positive neurons detected. The same protocol was applied for counting cholinergic nervus terminalis neurons co-labeled with ACE2. A total number of 119 GnRH-positive neurons and a total of 52 CHAT-positive neurons were counted from 3 to 5 animals, as shown in detail in Supplementary Table 2. The results were analyzed using GraphPad Prism software. Results are presented as mean ± standard error of the mean (SEM). An unpaired t-test was applied to determine whether the difference in ACE2 colocalization between GnRH + and CHAT + neurons was statistically significant. For details of quantification, see Supplementary Table 2. TMPRSS2 and Cathepsin B and L Immunocytochemistry TMPRSS2 and cathepsins B and L are proteases that SARS-CoV-2 can use to gain entry into host cells (Shang et al., 2020). To determine whether nervus terminalis neurons also express TMPRSS2, tissue sections were incubated with TMPRSS2 antibodies at 1:50 or 1:200 dilution as recommended by the manufacturer. We tested one cathepsin B and one cathepsin L antibody and three different anti-TMPRSS2 antibodies (listed in Supplementary Table 1) to determine the presence of these proteases in GnRH-positive nervus terminalis neurons. The same protocol as described above for the ACE2 antibody was used. Quantification of GnRH and cathepsin L co-labeled neurons was performed using the same protocol as described for quantification of neurons labeled with ACE2.

RESULTS
It was previously shown that GnRH is a marker for a major fraction of nervus terminalis neurons (Jennes, 1987;Schwanzel-Fukuda et al., 1987;Demski, 1993;Kim et al., 1999;von Bartheld, 2004). Immunolabeling for GnRH in 3-4 week-old mice showed labeled neurons localized along the olfactory nerve between the olfactory epithelium and the olfactory bulbs (Figures 2A-H), as expected from previous studies in rodents (Schwanzel-Fukuda et al., 1986;Wirsig and Leonard, 1986;Schwanzel-Fukuda et al., 1987). The majority of the GnRHpositive nervus terminalis neurons was located along the midline in the posterior part of the olfactory epithelium and adjacent to the olfactory bulbs. Preliminary examination revealed that these cells were in the same vicinity as cells labeled with the ACE2 antibody (Figures 2A,B,E,F). The large majority of GnRH-positive nervus terminalis neurons were fusiform and unipolar in shape.  Figure 3A. Control sections probed without primary antibodies or with control rabbit IgG had no detectable signal (not shown). Arrows and triangles indicate double-labeled neurons or lack thereof. Scale bars: 20 µm.

Most GnRH + Nervus Terminalis Neurons Express ACE2
To determine whether some neurons of the nervus terminalis contained both GnRH and ACE2, and to estimate the number of such neurons, we performed double immunolabeling experiments, and single-and double-labeled cells were counted on 15-20 sections from five different animals. The analyzed olfactory epithelium and olfactory bulb region and section's cutting plane are as indicated in Figure 1. Out of 119 GnRH + neurons, 107 (89.9%) were double-labeled for ACE2 ( Figure 3A). The intensity (0-250 scale) of ACE2 immunolabel in nervus terminalis neurons (range of 63-114, mean = 86) was comparable to the intensity of ACE2 immunolabel (range of 70-141, mean = 108) that we have previously demonstrated for sustentacular cells in the olfactory epithelium in some of the same tissue sections, and with the same protocol . Controls included omission of the primary antibody (not shown) and double immunofluorescent reactions performed using cryosections derived from an ACE2 knockout mouse as shown in Figures 2I-L. The GnRH-positive neurons were never labeled for olfactory marker protein (OMP), a marker for mature olfactory receptor neurons (Supplementary Figure 1). The total number of GnRH + nervus terminalis neurons per mouse was estimated to be approximately 125, which is very similar to a previous serial section analysis in hamster (about 130-140 GnRH + neurons, Wirsig and Leonard, 1986).

A Small Fraction of CHAT + Nervus Terminalis Neurons Express ACE2
The nervus terminalis complex is comprised of several distinct heterogenous populations of neurons. In addition to GnRH neurons which form the major fraction of nervus terminalis neurons, the second largest nervus terminalis subpopulation are cholinergic neurons that can be identified by the presence of choline acetyltransferase (CHAT) or cholinesterase (Wirsig and Leonard, 1986;Demski, 1993;von Bartheld, 2004). Therefore, CHAT neurons were also double-labeled with ACE2 (Figures 2M-P), and the fraction of CHAT-positive and ACE2positive neurons was estimated out of a total of 52 CHAT-positive neurons in three animals. In contrast to GnRH + /ACE2 + cells, only a minor fraction (9.4%) of CHAT-positive neurons were labeled with ACE2 which indicates that relatively few cholinergic The large majority of GnRH-positive neurons is also ACE2-positive. In contrast, the majority of CHAT-positive (cholinergic) nervus terminalis neurons lack ACE2-expression. The total number of counted GnRH-positive or CHAT-positive neurons was set at 100%. Error bars represent ± SEM. A t-test shows that the colocalization difference between GnRH-and CHAT-positive nervus terminalis neurons is significant at p < 0.0001. For further details, see Supplementary Table 2. (B) Western blot of ACE2 in wildtype (wt) mice and in ACE2 knock-out (KO) mice. The first two lanes (kidney) were loaded with 25 µg total protein, the lanes for olfactory bulb and cerebral cortex were loaded with 60 µg total protein, and probed with the R&D ACE2 antibody. No ACE2 protein was detectable in the ACE2 KO mice, proving that the antibody indeed recognizes ACE2. nervus terminalis neurons express ACE2 protein ( Figure 3A and Supplementary Table 2). Most of the CHAT-positive and also ACE2-positive nervus terminalis neurons were fusiform and unipolar in shape. Control experiments included omission of primary antibody (not shown) and double immunofluorescent reactions performed using cryosections derived from ACE2 knockout mouse (see below). The total number of CHATpositive nervus terminalis neurons per mouse was estimated to be approximately 60-70. This is less than the 130-140 acetylcholinesterase containing nervus terminalis neurons in hamster (Wirsig and Leonard, 1986), but it is known that only a fraction of neurons containing acetylcholinesterase actually are cholinergic (Schwanzel-Fukuda et al., 1986). The difference in colocalization of ACE2 between the two transmitter phenotypes of nervus terminalis neurons was highly significant, indicating that ACE2 expression is not randomly distributed in the nervus terminalis system.

Proof of the Specificity of the ACE2 Antibody: Western Blots From ACE2 −/− Mice
Immunolabeling experiments did not reveal any signal beyond background when the primary antibodies were omitted. For more precise visualization of background ACE2 staining, tissue derived from ACE2 knock-out mouse was used. In Western blots, the ACE2-specific band was absent in total protein extract obtained from ACE2 −/− animals ( Figure 3B). Therefore, sections from ACE2 −/− mice were also used for double immunolabeling experiments with ACE2 antibody and results showed that, as expected, GnRH-positive neurons were negative for ACE2 in tissue sections from the knock-out animals (Figures 2I-L).

The Protease TMPRSS2 Is Not or Minimally Expressed in Nervus Terminalis Neurons
TMPRSS2 is one of several proteases that cleave the spike protein, allowing SARS-CoV-2 to enter the host cell (Shang et al., 2020). Our attempts to double-label nervus terminalis neurons using three different anti-TMPRSS2 antibodies (Supplementary Table 1) resulted in no specific label above background in GnRHpositive nervus terminalis neurons (Supplementary Figure 1E). Levels of TMPRSS2 expression in these neurons may be too low for detection, or TMPRSS2 is not expressed by either the GnRHor CHAT-expressing nervus terminalis neurons.

The Proteases Cathepsins B and L Are Expressed in Nervus Terminalis Neurons
It is known that endolysosomal proteases cathepsin B and L can also enhance entry of SARS-CoV-2 to host cells (Gomes et al., 2020;Bollavaram et al., 2021;Zhao et al., 2021). To determine whether some neurons of the nervus terminalis contained both GnRH and cathepsin B or L, and to estimate the number of such cells, we performed double immunolabeling experiments (Figures 3C-F), and single-and double-labeled cells were counted on 15-20 sections from three different animals. The analyzed olfactory epithelium and olfactory bulb region and section's cutting plane are as indicated in Figure 1. After surveying a total of 51 GnRH positive neurons, we found that 90.0% of them were double-labeled for cathepsin L (Cath L, Figure 3A). A similar trend was observed for cathepsin B with a large proportion of GnRH-positive neurons co-labeled with the two antibodies (data not shown).

DISCUSSION
Our experiments confirmed the locations and approximate numbers of GnRH-positive neurons of the nervus terminalis in rodents (Schwanzel-Fukuda et al., 1986;Wirsig and Leonard, 1986;Schwanzel-Fukuda et al., 1987). In mouse, we found that the number of CHAT-positive neurons was about half of the number of GnRH-positive neurons. Interestingly, the large majority of GnRH-positive neurons expressed ACE2 and cathepsin B or L, while only a small fraction of CHAT-positive neurons co-localized ACE2. Previous studies have suggested that a larger fraction of the CHAT-positive neurons were multipolar and possibly associated with an autonomic function, while GnRH-positive neurons are thought to be sensory and/or may have neurosecretory functions (Schwanzel-Fukuda et al., 1986;Wirsig and Leonard, 1986).
Mice have been most often used as model systems for ACE2 expression, for localization of SARS-CoV-2 in the olfactory epithelium, and to study neuro-invasion of the brain along the olfactory route Cooper et al., 2020;Rathnasinghe et al., 2020). Mice have the advantage that a large number of mutants are available (Butowt and von Bartheld, 2020), but they normally express an ACE2 version that binds SARS-CoV-2 with low affinity (Damas et al., 2020). Therefore, to study SARS-CoV-2 infection in mice, a mouse-adapted virus has to be used (Leist et al., 2020), or mice have to be engineered to express human ACE2 (Butowt et al., 2021).
SARS-CoV-2 uses ACE2 to bind to host cells, and then the spike protein is cleaved by surface or endosomal proteases to facilitate virus entry. One of the more common proteases to facilitate SARS-CoV-2 entry is TMPRSS2 (Shang et al., 2020). TMPRSS2 is minimally expressed in adult neurons (Paoloni-Giacobino et al., 1997), including GnRH-expressing neurons in the brain (Ubuka et al., 2018). Similarly, TMPRSS2 was minimally or not at all expressed in nervus terminalis neurons. TMPRSS2 is only one of several proteases that are known to cleave the spike protein of SARS-CoV-2. Additional proteases that can facilitate SARS-CoV-2 entry into host cells in the absence of TMPRSS2 include cathepsins B and L Gomes et al., 2020;Bollavaram et al., 2021;Zhao et al., 2021), and also furin (Masre et al., 2020). Pre-activation by furin enhances viral entry in cells that lack TMPRSS2 or cathepsins, or have low levels of expression of these proteases (Shang et al., 2020). Furin is a ubiquitously expressed protease with a fundamental role in maturation of proteins in the secretory pathway; it is expressed in virtually all cells in rodent brain albeit at different levels (Day et al., 1993). Furin is known to be expressed in Bowman gland cells (Ueha et al., 2021), and therefore would not be a limiting factor for SARS-CoV-2 entry into innervating nervus terminalis neurons. Accordingly, lack of TMPRSS2 in nervus terminalis neurons does not negate the possibility of SARS-CoV-2 infecting these neurons after using alternative proteases to gain entry.
Our finding of expression of ACE2 and cathepsins B and L in the large majority of GnRH-expressing nervus terminalis neurons suggests that this cranial nerve is a more plausible conduit for brain infection than the olfactory neurons that entirely or for the most part lack ACE2 expression Cooper et al., 2020;Klingenstein et al., 2021). The nervus terminalis neurons may obtain the SARS-CoV-2 directly from infected cells in Bowman's glands, or through free nerve endings within the olfactory epithelium, many parts of which degenerate when sustentacular cells are infected by SARS-CoV-2 (Bryche et al., 2020). The lack of ACE2 in olfactory receptor neurons (except for those in the Grueneberg ganglion, Brechbühl et al., 2021) appears to be an effective barrier to virus transfer along  Larsell (1950), CSF contacts according to Jennes (1987). Cells expressing ACE2 are indicated in green, including sustentacular cells (SUS) and BG cells. Both of these cell types have been shown to express ACE2 Brann et al., 2020;Chen et al., 2020;Ye et al., 2020;Zhang et al., 2020;Klingenstein et al., 2021). Cell types that have been documented to be infected by SARS-CoV-2 are indicated with pink asterisks. SARS-CoV-2 localization in SUS cells according to Bryche et al. (2020); Leist et al. (2020), Ye et al. (2020); Zhang et al. (2020), Zheng et al. (2021);de Melo et al. (2021), and in BG cells according to Bryche et al. (2020); Leist et al. (2020), and Ye et al. (2020). BG cells furthermore express the protease furin (Ueha et al., 2021) which may facilitate virus entry into those nervus terminalis neurons which innervate BG cells. the olfactory nerve (Butowt et al., 2021). The nervus terminalis, however, has multiple venues to become infected by the virus, as illustrated in Figure 4.
Another important aspect is that the timeline of appearance of SARS-CoV-2 in the brain fits the nervus terminalis projections, with an explosive appearance of the virus in the forebrain in some mouse models (Winkler et al., 2020;Zhou et al., 2020;Carossino et al., 2021;Zheng et al., 2021), rather than a gradual transfer along the olfactory projections as would be expected from a virus that gains access to the brain exclusively via olfactory projections (Barnett and Perlman, 1993;Netland et al., 2008). The nervus terminalis has direct projections into the forebrain, reaching as far caudal as the hypothalamus (von Bartheld, 2004), and if the virus indeed infects these neurons, this could explain why the virus reaches the brain and cerebrospinal fluid (CSF) spaces much faster than seems possible via "neuron hopping" along olfactory projections. Most of the virus-containing axons in the olfactory nerve demonstrated by de Melo et al. (2021) do not express olfactory marker protein, suggesting that they are not axons belonging to olfactory receptor neurons, and therefore may be nervus terminalis axons which also project along the olfactory nerve (Larsell, 1950).
On a comparative note, since dolphins and whales have a much larger number of nervus terminalis neurons than any other vertebrates (Oelschläger et al., 1987), and these marine mammals express ACE2 that is highly susceptible to SARS-CoV-2 binding (Damas et al., 2020), our finding of ACE2 in nervus terminalis neurons suggests that these animals may be more vulnerable to brain infection via the nervus terminalis-even in the absence of an olfactory system.
Another argument to consider the nervus terminalis as an alternative to the olfactory route is that neuro-invasion in most animal models is highly variable, even in the same species and transgenic model (Jiang et al., 2020;Oladunni et al., 2020;Rathnasinghe et al., 2020;Winkler et al., 2020;Ye et al., 2020;Zhou et al., 2020;Zheng et al., 2021), and this is in contrast to the olfactory system that is consistent in terms of numbers of neurons, gene expression and projections. The nervus terminalis, on the other hand, is known for its large variability between individuals of the same species or even when comparing the right side with the left side of the same individual (Larsell, 1918;Jin et al., 2019). Such numerical differences can approach or even exceed an entire order of magnitude Jin et al., 2019)-and thus may explain the reported large variability in neuro-invasion (Butowt et al., 2021). Taken together, nervus terminalis neurons, for the above reasons, should be considered as a plausible alternative to the olfactory projections for neuro-invasion of SARS-CoV-2 from the nose to the brain in COVID-19. Whether the virus can indeed infect nervus terminalis neurons cannot be deduced by protein expression, but has to be determined by experiments using infectious SARS-CoV-2 after nasal inoculation in experimental animal models, or by demonstration of SARS-CoV-2 in nervus terminalis neurons in tissues from patients with COVID-19.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

ETHICS STATEMENT
The animal study was reviewed and approved by the Local Ethics Committee in Bydgoszcz.

SUPPLEMENTARY MATERIAL
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fncel.

2021.674123/full#supplementary-material
Supplementary Figure 1 | Examples of double immunofluorescent labeling of nervus terminalis neurons with the markers GnRH and olfactory marker protein (OMP) (A-D), and GnRH and TMPRSS2 (E). Label for GnRH (A) and OMP (B) in the medial region adjacent to the olfactory bulbs as indicated in Figure 1. Nuclei are stained with Hoechst 33258 (C) and the merged image is shown in (D).
GnRH-labeled cells were never labeled for OMP in this region. (E) GnRH-labeled nervus terminalis neurons (one neuron indicated with the white asterisk) did not co-localize with cells positive for TMPRSS2 (white arrow). Scale bars: 20 µ m.
Supplementary Table 2 | Numbers of animals, sections, and numbers of neurons double-labeled for nervus terminalis markers (GnRH, CHAT) and ACE2.