AUTHOR=Xu Bojie , Chen Jiaqi , Fu Jiyang , Yang Ruicheng , Yang Bo , Huo Dong , Tan Chen , Chen Huanchun , Wang Xiangru 

TITLE=Meningitic Escherichia coli-Induced Interleukin-17A Facilitates Blood–Brain Barrier Disruption via Inhibiting Proteinase 3/Protease-Activated Receptor 2 Axis

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=16

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2022.814867

DOI=10.3389/fncel.2022.814867

ISSN=1662-5102

ABSTRACT=<p>Bacterial meningitis is a life-threatening infectious disease with high morbidity and mortality worldwide, among which meningitic <italic>Escherichia coli</italic> is a common Gram-negative pathogenic bacterium causing meningitis. It can penetrate the blood–brain barrier (BBB), invoke local inflammatory responses and consequently disrupt the integrity of the BBB. Interleukin-17A (IL-17A) is recognized as a pro-inflammatory cytokine that is released during meningitic <italic>E. coli</italic> infection. It has been reported that IL-17A is involved in several pathological tissue injuries. However, the function of IL-17A in BBB breakdown remains rarely discussed. Here, our study found that <italic>E. coli</italic>-induced IL-17A led to the degradation of tight junction proteins (TJs) and adherens junction proteins (AJs) in human brain microvascular endothelial cells (hBMECs) through inhibiting protease proteinase 3 (PRTN3)/protease-activated receptor 2 (PAR-2) axis, thus increasing the permeability of BBB. In summary, this study uncovered the involvement of IL-17A in regulating BBB integrity and proposed a novel regulatory mechanism, which could be potential therapeutic targets of <italic>E. coli</italic> meningitis.</p>