Cell-type specific regulation of cortical excitability through the allatostatin receptor system

Recent technical advances enable the regulation of neuronal circuit activity with high spatial and temporal resolution through genetic delivery of molecular activation or inactivation systems.Among them, the allatostatin receptor (AlstR)/ligand system has been developed for selective and quickly reversible silencing of mammalian neurons. However, targeted AlstR-mediated inactivation of specific neuronal types, particularly diverse types of inhibitory interneurons, remains to be established. In the present study, we achieved Cre-directed expression of AlstRs to excitatory and inhibitory cell-types in the cortex, and found that the AlstR-mediated inactivation was specific and robust at single-cell and neuronal population levels. Bath application of the allatostatin peptide markedly reduced spiking activity of AlstR-expressing excitatory and inhibitory neurons in response to intrasomatic current injections and laser photostimulation via glutamate uncaging, but control neurons without AlstR expression were not affected. As for the cortical network activity, the peptide application constrained photostimulation-evoked excitatory activity propagation detected by fast voltage-sensitive dye (VSD) imaging of the slices expressing AlstRs selectively in excitatory neurons, while it augmented excitatory activity in those slices with inhibitory neurons expressing AlstRs. In addition, AlstR-mediated inactivation effectively suppressed pharmacologically induced seizure activity in the slices targeting AlstRs to excitatory neurons. Taken together, our work demonstrated that the genetic delivery of AlstRs can be used for regulation of cortical excitability in a cell-type specific manner, and suggested that the AlstR system can be potentially used for fast seizure control.


INTRODUCTION
In the last decade, there has been considerable progress in the development and use of molecular and genetic methods for studying the central nervous system (Luo et al., 2008). Genetic strategies for activating or inactivating selected neurons have opened up new possibilities for understanding neuronal circuitry with high spatial and temporal resolution (Lechner et al., 2002;Boyden et al., 2005;Karpova et al., 2005;Tan et al., 2006;Armbruster et al., 2007;Lerchner et al., 2007;Chow et al., 2010). Along with other newly developed molecular techniques, the allatostatin receptor (AlstR) inactivation system can be a potent tool for regulating neocortical circuit activity with a high degree of specificity and effectiveness. The AlstR and its ligand peptide, allatostatin (AL), are found in insect brains, not native to mammalian neuronal tissue (Birgul et al., 1999). When expressed in mammalian neurons through genetic methods (Lechner et al., 2002;Gosgnach et al., 2006;Tan et al., 2006Tan et al., , 2008Wehr et al., 2009), the receptor system can been used for silencing of neuronal activity as the application of the exogenous ligand quickly increases K + channel conductance and keeps membrane potentials from depolarizing above threshold through G protein-coupled AlstRs, which gate endogenous G protein-coupled inward-rectifying K + (GIRK) channels in AlstRexpressing neurons. The AlstR/AL-mediated inactivation is estimated to occur within seconds to minutes, depending on the peptide delivery speed (Lechner et al., 2002;Tan et al., 2006). Endogenous expression of GIRK channels is common to the great majority of mature neurons in the mammalian brain (Karschin et al., 1996), such that cotransfection with GIRK channel subunits is unnecessary to effectively inactivate neurons using the AlstR/AL system (Lerchner et al., 2007;Wehr et al., 2009). Because mammals do not express the AL peptide or its receptor, peptide application should specifically inactivate only the neurons expressing the AlstR transgene.
Selective AlstR expression and its neuronal inactivation in the brains of mice, ferrets, and monkeys has been demonstrated via viral infection or using transgenic approaches Tan et al., 2006Tan et al., , 2008Wehr et al., 2009). However, targeted AlstR-mediated inactivation of specific neuronal types, particularly diverse types of inhibitory interneurons in the neocortex, remains to be established. Given that there are multiple cell-types and each type is likely to have a unique role in cortical circuits, in the present study, we aimed to express the AlstR system to excitatory or inhibitory cell-types for cell-type selective regulation of cortical excitability through a double transgenic mouse approach. The transgenic mouse line conditionally expressing AlstR following Cre recombination (see below) was cross-bred with an Emx1-Cre mouse line (expressing Cre recombinase in most excitatory neurons of the cortex) (Guo et al., 2000;Kuhlman and Huang, 2008) or a Dlx5/6-Cre mouse line (expressing Cre in forebrain GABAergic neurons) for selective delivery of AlstRs to either excitatory or inhibitory cell-types in the brain.
We combined electrophysiology, laser scanning photostimulation, and fast voltage-sensitive dye (VSD) imaging to examine AlstR-mediated regulation of cortical excitability in living brain slice preparations, and addressed the following questions: (a) How effective is the Cre-mediated, selective expression of AlstRs? (b) Is the AlstR-mediated inactivation specific or robust at singlecell and neuronal population levels? (c) Can the AlstR system be used to suppress seizure activity when targeting AlstR expression to excitatory neurons? Our experiments demonstrated that the AlstR system can be effectively used for regulation of cortical excitability in a cell-type specific manner, and suggested that the AlstR system can be developed for potential translational applications in seizure control.

SLICE PREPARATION
All animals were handled and experiments were conducted in accordance with procedures approved by the Institutional Animal Care and Use Committee at the University of California, Irvine. In this study, we used a newer AlstR mouse line conditionally expressing AlstR and green fluorescent protein (GFP) following Cre recombination. This AlstR mouse line (R26-AlstR) was created by using the same targeting vector of transgene as the AlstR 192 strain , but the vector was inserted at the Rosa26 locus to drive more stable expression. Specifically, the R26-AlstR mouse was first generated using a conditional double-stop cassette system that has been described previously (Kim et al., 2009). The AlstR gene followed by an IRES-EGFP cassette was placed downstream of the CAG promoter and FSF-LSL double-stop cassette. The Cre-dependent R26-AlstR mouse line was then produced by removing the FSF stop cassette by crossing the original double-stop AlstR mouse with the mouse line of ACTB-FLPe transgene (Rodriguez et al., 2000). To achieve Cre-directed expression of AlstRs, the R26-AlstR mouse line was cross-bred with an Emx1-Cre mouse line (Guo et al., 2000) or a Dlx5/6-Cre mouse line (Monory et al., 2006). Emx1 is a mouse homologue of the Drosophila homeobox gene empty spiracles and its expression is largely restricted to excitatory neurons in the developing and adult cerebral cortex and hippocampus (Guo et al., 2000;Gorski et al., 2002;Kuhlman and Huang, 2008); the Emx1-Cre mouse line was created with the Cre gene placed directly downstream of the Emx1 promoter (Guo et al., 2000). Dlx is a family of homeodomain transcription factors which are related to the Drosophila distal-less (Dll) gene (Panganiban and Rubenstein, 2002); in the Dlx5/6 Cre mouse, expression of Cre is directed to virtually all forebrain GABAergic neurons during embryonic development by the I56i and I56ii enhancers from the zebrafish dlx5a/dlx6a intergenic region (Monory et al., 2006).
To prepare living brain slices, the double transgenic mouse pups (postnatal day 16-21) or control pups (AlstR or Cre mouse pups) were deeply anesthetized with pentobarbital sodium (>100 mg/kg, i.p.) and rapidly decapitated, and their brains removed. For electrophysiological experiments, primary somatosensory (S1) cortical slices of 400 µm thick were cut in sucrose-containing artificial cerebrospinal fluid (ACSF) (in mM: 85 NaCl, 75 sucrose, 2.5 KCl, 25 glucose, 1.25 NaH 2 PO 4 , 4 MgCl 2 , 0.5 CaCl 2 , and 24 NaHCO 3 ). Slices were first incubated in sucrose-containing ACSF for 30 minutes-one hour at 32 • C, and then transferred to recording ACSF (in mM: 126 NaCl, 2.5 KCl, 26 NaHCO 3 , 2 CaCl 2 , 2 MgCl 2 , 1.25 NaH 2 PO 4 , and 10 glucose). For VSD imaging experiments, cortical slices were cut in ACSF with a broad-spectrum excitatory amino acid antagonist kynurenic acid (0.2 mM). The ACSF with kynurenic acid was found to better preserve slice healthiness for dye staining than the sucrose-containing ACSF. After the initial incubation at 32 • C, slices were transferred for dye staining at room temperature for one hour in the ACSF and 0.1 mg/ml of the absorption VSD, NK3630 (Nippon Kankoh-Shikiso Kenkyusho Co. Ltd., Japan) and then maintained in the recording ACSF before use. Throughout the cutting, incubation, staining, and recording, the solutions were continuously supplied with 95% O 2 -5% CO 2 .

ELECTROPHYSIOLOGY, LASER SCANNING PHOTOSTIMULATION AND VOLTAGE-SENSITIVE DYE IMAGING
Our overall system of electrophysiological recordings, photostimulation, and imaging was described previously (Xu et al., 2010;Xu, 2011). The solution was fed into the slice recording chamber through a pressure driven flow system with pressurized 95% O 2 -5% CO 2 with a perfusion flow rate of about 2 ml/minute. To perform whole-cell recording, individual neurons were visualized at high magnification (60x objective), and were patched with glass electrodes of 4-6 M resistance that were filled with an internal solution containing (in mM) 126 K-gluconate, 4 KCl, 10 HEPES, 4 ATP-Mg, 0.3 GTP-Na, and 10 phosphocreatine (pH 7.2, 300-305 mOsm). The internal solution also contained 0.1% biocytin for cell labeling and morphological identification. Once stable whole-cell recordings were achieved with good access resistance (usually <20 M ), basic electrophysiological properties were examined through hyperpolarizing and depolarizing current injections. We focused on analyzing neuronal input resistance, spiking thresholds, and spike numbers at different steps of depolarizing current pulses in control ACSF, in the presence of AL, and after washout of AL. To examine AlstR-mediated inactivation, the ligand AL was added into the recording solution. Although nanomolar concentrations of AL could inactivated isolated cultured AlstR-expressing neurons (Lechner et al., 2002), 1 µM AL (unless specified otherwise) were used for facilitating the ligand penetration deep into the slices and increasing temporal speeds of the ligand infusion. The AL application for 15 minutes was estimated to produce full AlstR effects, while the washout of 20 minutes was considered to remove the added AL from the recording solution.
During laser scanning photostimulation experiments, the microscope objective was switched from 60x to 4x. Stock solution Frontiers in Neural Circuits www.frontiersin.org of MNI-caged-l-glutamate (Tocris Bioscience, Ellisville, MO) was added to 20 ml of ACSF for a concentration of 0.2 mM caged glutamate. The cortical slice image was acquired by a high resolution digital CCD camera, which in turn was used for guiding and registering photostimulation sites. A laser unit (DPSS Lasers, Santa Clara, CA) was used to generate 355 nm UV laser for glutamate uncaging. Short pulses of laser flashes (1 ms, 20 mW) were controlled by using an electro-optical modulator and a mechanical shutter. The laser beam formed uncaging spots, each approximating a Gaussian profile with a width of ∼100 µm laterally at the focal plane. During the experiments examining neuronal excitation profiles, photostimulation was applied to 8 × 8 patterned sites (with an inter-site space of 50 µm 2 ) centered at the recorded neurons in a non-raster, non-random sequence to avoid revisiting the vicinity of recently stimulated sites, while current-clamp recordings measured membrane potential depolarization and spiking activity. The neuronal excitation profile was simply assessed by the number of spiking locations to the photostimulation of 8 × 8 sites. Laser scanning photostimulation permits rapid evaluation of multiple stimulation locations with no physical damage to the tissue under our experimental conditions. During VSD imaging experiments, a 705 nm light transilluminated brain slices and voltage-dependent changes in the light absorbance of the dye were captured by the MiCAM02 fast imaging system (SciMedia USA Ltd, Costa Mesa, CA). The illumination was supplied by optically filtering white light produced by an Olympus tungsten-halogen lamp up to 100 W of power. Optical recording of VSD signals was performed under the 2x objective with a sampling rate of 2.2 or 4.4 ms per frame (frame resolution 88 (w) × 60 (h) pixels). The field of view covered the area of 2.56 × 2.14 mm 2 with a spatial resolution of 29.2 × 35.8 µm 2 /pixel. For the imaging of photostimulationevoked excitatory activity with and without the AL application, VSD imaging of evoked activity was triggered and synchronized with each laser photostimulation at specified cortical sites. For each trial, the VSD imaging duration was 2000 frames including 500 baseline frames with an inter-trial interval of 12-14 seconds. To image pharmacologically induced spontaneous seizure-like activity, ACSF with 10 µM bicuculline were infused into the slices for 15 minutes before VSD imaging; the imaging experiments consisted of seven sessions each at three perfusion conditions: bicuculline only, bicuculline + AL, and bicuculline only again in ACSF. Each session had nine consecutive trials conducted with three minutes of off-illumination intervals between sessions. The number of seizure occurrences per session was measured under different conditions. VSD signals were originally measured by the percent change in pixel light intensity [ I/I%; the % change in the intensity ( I) at each pixel relative to the initial intensity (I)]. In addition, the mean and standard deviation (SD) of the baseline activity of each pixel across the 50 frames before the spontaneous activity onset or preceding photostimulation was calculated, and VSD signal amplitudes were then expressed as SD multiples above the mean baseline signal for display and quantification. The activated pixel was empirically defined as the pixel with the amplitude ≥ 1 SD above the baseline mean of the corresponding pixel's amplitude (equivalent to the detectable signal level in the original VSD maps of I/I%). VSD images were smoothed by convolution with a Gaussian spatial filter (kernel size: five pixels; SD (σ ): one pixel), and a Gaussian temporal filter (kernel size: three frames; δ: one frame). In the present study, single-trial VSD signals were of sufficiently high amplitudes and could be discerned from background noise; no averaging over multiple trials was used for data presentation unless specified. To examine AlstR-mediated effects on photostimulation-evoked VSD responses, the total numbers of activated pixels and response amplitudes across the 10 frames around the peak VSD activation (e.g., at 55 ms after photostimulation) were measured for comparison across control, AL application, and washout. Images were displayed and analyzed using custom-made MATLAB programs.

STATISTICAL ANALYSIS
For statistical comparisons across more than two groups, we used the Kruskal-Wallis test (non-parametric One-Way ANOVA) and the Mann-Whitney U-test for group comparisons. Alpha levels of p ≤ 0.05 were considered significant. All the values were presented as mean ± SE.

SELECTIVE EXPRESSION OF AlstRs AND CHARACTERIZATION OF AlstR-MEDIATED NEURONAL INACTIVATION
In the present study, we used Cre-directed expression of the AlstR transgene to excitatory and inhibitory cortical neurons by crossing the R26-AlstR mouse line conditionally expressing AlstR and GFP following Cre recombination  with the Emx1-Cre mouse line (Guo et al., 2000) or the Dlx5/6-Cre mouse line (Monory et al., 2006). We confirmed the specificity and efficiency of these Cre mouse lines in achieving cell-type specific gene expression. As demonstrated in Figures 1A-F, through the use of a tdTomato Cre reporter line (Madisen et al., 2010), the Emx1-Cre mouse expresses Cre in a majority of excitatory pyramidal neurons of the cortex (Guo et al., 2000;Gorski et al., 2002) (Figures 1A-C), while the Dlx5/6 mouse expresses Cre specifically in inhibitory interneurons (Monory et al., 2006;Chao et al., 2010) (Figures 1D-F).
Emx1-cre: AlstR mice expressed AlstRs with GFP in cortical excitatory neurons, which was verified through GABA immunostaining (Figures 1G,H) and electrophysiological recordings (see below). Immunochemical examinations and living slice observations indicated that about 50% of excitatory cortical neurons express AlstRs, detected by their strong GFP appearance. Similarly, AlstR expression in inhibitory cortical neurons of Dlx5/6-Cre: AlstR mice was strong, as the native fluorescence enabled direct visualization and targeted electrophysiological recordings of the labeled neurons.
We started to test whether AlstR expression through the transgenic mouse approach led to functional inactivation of neuronal activity with single-cell electrophysiology. As illustrated in Figures 2A-D current injections in Emx1-Cre: AlstR mouse slices. Similar to previous findings (Lechner et al., 2002;Wehr et al., 2009), bath application of AL hyperpolarized resting membrane potentials, markedly reduced membrane input resistance of AlstR-expressing (AlstR+) neurons (Figures 2B,D), and inhibited action potential firing by the decreased spike numbers elicited across a range of depolarizing current injections (Figures 2B,C). Control neurons without AlstR expression were Frontiers in Neural Circuits www.frontiersin.org not affected (N = 10). Then we further characterized AlstRmediated inhibition of evoked excitability by laser scanning photostimulation. Compared to the examination of intrinsic membrane excitability through current injections, the measurement of photostimulation-evoked neuronal excitability via glutamate uncaging is more physiologically meaningful as the evoked excitability depends on the structure and membrane characteristics of the recorded neurons, and reflects the combination of intrinsic neuronal excitability and local synaptic input. Caged glutamate was present in the bath solution, and only turned active through focal UV photolysis. As shown in Figure 2E, we recorded from individual neurons and photostimulation was applied across multiple sites. Our data established that the AlstR-mediated inactivation was sufficiently strong and it essentially abolished the photostimulation-evoked spiking activity of the recorded AlstR-expressing excitatory neurons (Figures 2F-I).
As for inhibitory cortical neurons, we showed that the AlstR system produced robust inhibition on major subtypes of inhibitory cortical neurons through examinations of AlstRexpressing inhibitory neurons in Dlx5/6-Cre: AlstR mouse slices. The electrophysiological phenotype of the inhibitory neurons Frontiers in Neural Circuits www.frontiersin.org examined was fast-spiking, adapting-spiking, low-threshold spiking, or late-spiking; the cells had morphologies resembling basket cells, Martinotti cells, bitufted/bipolar cells, or neurogliaform cells. Compared to excitatory neurons, bath application of AL had a much larger hyperpolarizing effect on the resting membrane potentials of AlstR-expressing inhibitory neurons (Figures 3A,B). AL application consistently inhibited all the inhibitory neurons tested by decreasing their intrinsic excitability to current injections ( Figure 3C) and photostimulation-evoked excitability (Figures 3D-L). It should be noted that the level of AlstRmediated inhibition varied with cell-types. For example, the spiking activity of AlstR+, fast-spiking cells tended to be suppressed completely while AlstR+, low-threshold spiking cells had remaining spikes in the presence of AL (Figures 3E-L). However, the overall effect of inhibition was substantial across interneuronal subtypes (Figures 3C,D).

AlstR-MEDIATED EFFECTS ON NEURONAL POPULATION ACTIVITY
After the examination of AlstR-mediated effects at the level of individual neurons, we went further to characterize the AlstR-mediated inhibition on neuronal population activity with our newly developed functional imaging technique. This is important because cortical circuits ultimately operate at the neuronal population level. Optical imaging with fast VSD was used to examine photostimulation-evoked population activity and to evaluate AlstR-mediated inhibition on cortical network activity. The VSD imaging can monitor neuronal activity in a large area simultaneously (Xu et al., 2010;Xu, 2011), with changes in optical absorption closely correlating with neuronal membrane depolarization. The brain slices were stained with VSD and perfused with ACSF containing caged glutamate. Then local cortical regions were stimulated with laser photostimulation, and circuit activation and output directly visualized by fast VSD imaging. For shows photostimulation-evoked activity of the recorded low-threshold-spiking neuron and a fast-spiking inhibitory neuron during control, AL application, and washout, respectively. The AL application substantially suppressed photostimulation-evoked spiking activity of AlstR-expressing inhibitory neurons. In (D), * * indicates statistical significance of p < 0.005 between control and AL application.
Frontiers in Neural Circuits www.frontiersin.org the purpose of visual display, in Figure 4 and subsequent figures, the color scale codes the strength of excitatory activity (reflecting membrane potential depolarization of neuronal ensembles) with warmer colors indicating greater excitation. In addition, the time course of VSD signal (in the percent change of pixel intensity [ I/I%]) from the specified region of interest (ROI) is shown with the image data. AlstR-mediated inhibition of excitatory neuronal population activity was found to be effective across different cortical sites (Figure 4). In Emx1-Cre: AlstR mouse slices, under control conditions, photostimulation in local cortical regions initiated excitation around 5 ms after laser exposure, which quickly propagated to functionally connected cortical regions within 50 ms in a columnar manner. Stimulation in the middle cortical layer (layer 4 or layer 5a) caused excitatory activity to spread vertically to layers 2/3, 5, and 6 ( Figures 4A1,A2 and  5A1,A2). Although photostimulation-evoked excitatory activity occurred in the presence of the AL peptide, evoked responses of the AlstR-expressing brain slice was clearly reduced and constrained (Figures 4B1,B2). Given that about half of excitatory cortical neurons expressed AlstRs in these slices, the AlstRmediated inhibition of excitatory population activity was quite effective. The AL effect was reversible with the peptide washout (Figures 4C1,C2). In comparison, AL application did not have effects on photostimulation-evoked population activity in the cortical slices without AlstR expression (N = 4). The reduction of VSD activation with the AL application for Emx1-Cre: AlstR slices, compared to control, is summarized in Figure 6A. On average, the total activation size during AL application was 18% ± 2.0% of the control condition; the total response amplitude during AL application was 15.8% ± 1.8% relative to control.
Conversely, we found that AlstR-mediated inactivation of GABAergic neurons augmented excitatory population activity. Photostimulation-evoked response in the presence of AL was dramatically increased in Dlx5/6-Cre: AlstR mouse slices (Figures 5A1,A2 and B1,B2), and the effect was reversed with the AL washout (Figures 5C1,C2). In agreement with the previous finding that S1 barrels are functionally independent cortical columns (Laaris et al., 2000;Petersen and Sakmann, 2001), in FIGURE 4 | AlstR-mediated inhibition of excitatory neuronal population activity in an Emx1-Cre: AlstR mouse slice. (A1) Time series data of VSD imaging of photostimulation-evoked activity in S1 cortex in normal ACSF. The color scale codes VSD signal amplitude expressed as standard deviation (SD) multiples above the mean baseline. The site of photostimulation between layers 4 and 5a can be identified by the laser excitation artifact (purple) in the first frame, which is also indicated by the white arrowhead. Color-coded excitatory activity is superimposed on the background slice image.

Frontiers in Neural Circuits
www.frontiersin.org normal ACSF, the lateral extent of photostimulation response in S1 layer 4 appeared to be limited to the cortical column defined structurally by the barrel (Figure 5A1). Yet, the importance of inhibitory neuronal regulation of cortical excitatory information flow is evident, as inhibition of GABAergic interneurons via the AlstR system caused the spread of excitation laterally in layer 4 as well as in wide regions of upper and deeper layers ( Figure 5B1). Across the slices, the increase of VSD activation in the AL presence, compared to control, was 500.5% ± 171% and 621.2% ± 272.6% for the total activation size and response amplitude, respectively ( Figure 6B).

THE USE OF AlstR SYSTEM FOR SUPPRESSING SEIZURE ACTIVITY
Given that targeted expression of AlstRs in excitatory neurons can strongly reduce cortical excitability with the ligand present, we were interested in investigating whether the AlstR system could be used for controlling seizure activity that is characterized by abnormal, excessive, or synchronous excitatory neuronal activity in the brain. We performed the experiments in single Emx1cre: AlstR slices sequentially. First, bath application of bicuculline induced spontaneous seizure-like activity in the slices. The epileptiform activity detected by VSD imaging, occurred in a small region and evolved into large propagating activity across the whole slice lasting for about 200-300 ms per event (Figures 7A1-A4 and B1,B2). After stable occurrences of the seizure activity across seven imaging sessions (126 seconds per session), AL peptide was co-applied with bicuculline and seizure occurrences continued to be monitored by VSD imaging. As illustrated in Figures 7C,D, the tendency of reduced seizure occurrences was clearly visible during the AL application. Note that the average durations or peak amplitudes of individual events during control and the AL infusion did not differ. Finally, the removal of AL from bath perfusion increased seizure occurrences, which could get back to the normal control level. For the Emx1-Cre: AlstR mouse slices tested, the average number of seizure occurrences per minute was 1.09 ± 0.14, 0.41 ± 0.12, 0.68 ± 0.38 for bicuculline-containing ACSF, co-application of bicuculline and AL, and bicuculline-containing ACSF again, respectively. The co-application of AL with bicuculline reduced the occurrence of bicuculline-induced seizure activity significantly ( Figure 7E). Thus, these data suggest that the AlstR system can be used to effectively suppress seizure activity.

DISCUSSION
In this study, we used a Cre-dependent transgenic mouse approach to effectively express AlstRs to specific types of cortical neurons. The experiments with single-cell electrophysiology, laser scanning photostimulation, and VSD imaging established that the AlstR-mediated inactivation of both excitatory and inhibitory neurons was specific and robust at single-cell and population levels. Our data also indicate that the AlstR system has good potential to be used as a molecular anticonvulsant for fast seizure control via cell-type specific expression.
As further demonstrated in this study, the AlstR/AL system has the required merits and applicable features as a potent molecular tool for regulation of cortical excitability. Optical inactivation of neurons can be effectively achieved by using the halorhodopsin or Arch system, which activates chloride or proton pumps and offers high temporal precision (Han and Boyden, 2007;Chow et al., 2010). Although no ligand is needed, prolonged optical silencing over the course of several minutes causes photo-toxicity and neuronal damage. In addition, optical illumination may interfere with functional imaging studies. One existing approach similar to the use of the non-native AlstR system is to genetically engineer G-protein-coupled receptors (GPCRs) of mammalian neurons (e.g., Receptors Activated Solely by Synthetic Ligands, or RASSLs) (Conklin et al., 2008;Alexander et al., 2009). For example, muscarinic acetylcholine receptors are engineered to generate a family of GPCRs that are activated solely by a pharmacologically inert drug-like compound (clozapine-N-oxide). The Gi-coupled designer receptor (hM4D) demonstrates its ability to induce membrane hyperpolarization and neuronal silencing in vitro and in vivo (Armbruster et al., 2007;Ferguson et al., 2011). The designed drug ligands can be administered in the periphery and cross the blood-brain barrier (BBB) to access receptors in deep brain structures and/or widely distributed neuronal populations. However, continued improvements are needed for the use of RASSLs, as the engineered receptors may be activated by non-selective, pharmacologically active small molecules. Furthermore, in some tissues RASSLs exhibit high levels of constitutive signaling in the absence of designed small-molecule agonists (Alexander et al., 2009), while the AlstR does not display evidence of basal signaling. Although the AL peptide is unlikely to cross the BBB in the current form and needs to be directly infused into brain tissue, future translational studies using the AL system will necessitate that AlstR ligands be designed with a capability of crossing the BBB. Systemic administration would likely result in some loss of temporal control, but it may still be sufficient to suppress excitatory activity of AlstR-expressing neurons. Please note that while the techniques for making BBB-permeable peptide analogs are established (Bickel et al., 2001;Egleton and Davis, 2005), the AL analogs for realistic human therapy will have to be developed toward this end.
A major focus of this work was to examine if the AlstR system can regulate the activity of diverse types of inhibitory cortical interneurons. Overall, inhibitory neurons can be inactivated, but compared to excitatory neurons, the degree of AlstR-mediated inhibition depended on interneuronal subtypes, which should be related to the factors including intrinsic neuronal excitability, AlstR expression levels, and endogenous GIRK densities. Given the availability of new interneuron-specific Cre driver lines developed by a NIH Neuroscience Blueprint Project in the laboratory of Josh Huang (available from the Jackson Laboratory) (Taniguchi et al., 2011), AlstR expression can be targeted to specific subsets of inhibitory cortical neurons for selectively suppressing their activity for future functional and behavioral studies.
In addition to the transgenic mouse approach, viral vectors can also be used for AlstR delivery in defined cell-types. Genetically modified adeno-associated viruses (AAV) and lentiviruses have been used for gene therapy in humans, enabling correcting certain genetic disorders in clinical trials (Davidson and Breakefield, 2003;Kootstra and Verma, 2003). The viral vectors can yield stable gene expression for months or years without apparent toxicity. Compared with the transgenic approach, major advantages of viral transfection include restricted expression in defined cortical regions, stronger AlstR expression levels with high-tier viruses, and the application to other species that transgenic approaches are impractical.
The AlstR approach represents an important advance for genetic manipulation of neuronal activity and can be valuable for many basic applications [see further discussion in (Callaway, 2005;Tan et al., 2006)]. We believe that the AlstR system can also Frontiers in Neural Circuits www.frontiersin.org in the presence of bicuculline, bicuculline and AL, and bicuculline again for two different example slices. Each imaging session consisted of nine consecutive trials of sampling 8.8 seconds per trial (with a 14 seconds inter-trial interval); the number of seizure events was measured for each session. A interval of 15-20 minutes was allowed for the AL infusion and washout before the next condition continued. (E) The data summary (N = 5 slices) showing that the co-application of AL with bicuculline (BIC) reduced the occurrence of bicuculline-induced seizure activity in Emx1-Cre: AlstR mouse slices. * Indicates statistical significance of p < 0.01 between BIC and BIC + AL.
Frontiers in Neural Circuits www.frontiersin.org be potentially used in translational applications, for example, in seizure control and epilepsy treatment. Despite the progress in antiepileptic drug development and surgical management, in about 30-40% of cases epileptic seizures do not respond well to the conventional therapies. We should capitalize on the development and use of new molecular and genetic tools to counteract seizure activity. Based upon the specificity and effectiveness of the AlstR system, this genetic inactivation system can be potentially developed as a molecular anticonvulsant with few side effects.