AUTHOR=Quirin Sean , Jackson Jesse , Peterka Darcy S., Yuste Rafael 

TITLE=Simultaneous imaging of neural activity in three dimensions

JOURNAL=Frontiers in Neural Circuits

VOLUME=8

YEAR=2014

URL=https://www.frontiersin.org/journals/neural-circuits/articles/10.3389/fncir.2014.00029

DOI=10.3389/fncir.2014.00029

ISSN=1662-5110

ABSTRACT=<p>We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity <italic>in vitro</italic> and <italic>in vivo</italic>. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping.</p>