%A Kim,Eun-Hee %A Yum,Mi-Sun %A Lee,Minyoung %A Kim,Eun-Jin %A Shim,Woo-Hyun %A Ko,Tae-Sung %D 2017 %J Frontiers in Neurology %C %F %G English %K Methylazoxymethanol Acetate,Malformations of Cortical Development,epileptic spasms,GABA,Fast oscillations %Q %R 10.3389/fneur.2017.00271 %W %L %M %P %7 %8 2017-June-12 %9 Original Research %+ Mi-Sun Yum,Department of Pediatrics, Asan Medical Center Children’s Hospital, University of Ulsan College of Medicine,Republic of Korea,yumyum99@daum.net %+ Tae-Sung Ko,Department of Pediatrics, Asan Medical Center Children’s Hospital, University of Ulsan College of Medicine,Republic of Korea,yumyum99@daum.net %# %! A New Rat Model of Epileptic Spasms %* %< %T A New Rat Model of Epileptic Spasms Based on Methylazoxymethanol-Induced Malformations of Cortical Development %U https://www.frontiersin.org/articles/10.3389/fneur.2017.00271 %V 8 %0 JOURNAL ARTICLE %@ 1664-2295 %X Malformations of cortical development (MCDs) can cause medically intractable epilepsies and cognitive disabilities in children. We developed a new model of MCD-associated epileptic spasms by treating rats prenatally with methylazoxymethanol acetate (MAM) to induce cortical malformations and postnatally with N-methyl-d-aspartate (NMDA) to induce spasms. To produce cortical malformations to infant rats, two dosages of MAM (15 mg/kg, intraperitoneally) were injected to pregnant rats at gestational day 15. In prenatally MAM-exposed rats and the controls, spasms were triggered by single (6 mg/kg on postnatal day 12 (P12) or 10 mg/kg on P13 or 15 mg/kg on P15) or multiple doses (P12, P13, and P15) of NMDA. In prenatally MAM-exposed rats with single NMDA-provoked spasms at P15, we obtain the intracranial electroencephalography and examine the pretreatment response to adrenocorticotropic hormone (ACTH) or vigabatrin. Rat pups prenatally exposed to MAM exhibited a significantly greater number of spasms in response to single and multiple postnatal NMDA doses than vehicle-exposed controls. Vigabatrin treatment prior to a single NMDA dose on P15 significantly suppressed spasms in MAM group rats (p < 0.05), while ACTH did not. The MAM group also showed significantly higher fast oscillation (25–100 Hz) power during NMDA-induced spasms than controls (p = 0.047). This new model of MCD-based epileptic spasms with corresponding features of human spasms will be valuable for future research of the developmental epilepsy.