Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia

Pain, as the most prevalent neurological complication of herpes zoster (HZ), may occur before or during the rash onset or even after the rash has recovered. Particularly, postherpetic neuralgia (PHN) is a refractory chronic condition, usually defined as pain persisting for 3 months or longer from the onset of HZ. Pain evoked by HZ impairs the normal physical and emotional functions of the patients, severely reducing their quality of life. However, how zoster-associated pain occurs and develops into PHN are elusive, making PHN difficult to predict. Uncovering the pathogenesis of zoster-associated pain (or HN) helps us to better understand the onset of PHN and supports developing more effective treatments. In this study, we successfully constructed a model for zoster-associated pain through varicella-zoster virus (VZV) infections of mouse footpads and pain behavior assessments. Next, we used the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Gene Ontology (GO) to analyze PHN rodent dorsal root ganglion (DRG) gene microarray data and found that calcium signal disorder might be involved in the onset of PHN. By using reverse transcription real-time fluorescent quantitative PCR (RT-qPCR) and Western blotting, we confirmed that VZV infection could significantly upregulate the expression of T-type calcium channel Cav3.2 in DRG and spinal dorsal horn (SDH). Intrathecal administration of Cav3.2 blocker (2R/S)-6-prenylnaringenin (6-PNG) relieved mechanical and thermal hyperalgesia induced by VZV. Taken together, our data indicated that VZV might participate in the occurrence and development of HN by upregulating the expression of Cav3.2 in DRG and SDH. These findings will help to reveal the underlying mechanisms on long-lasting pain and PHN formation, providing a new insight that Cav3.2 can be the promising drug target for remitting PHN.


INTRODUCTION
Herpes zoster (HZ) episodes, after the reactivation of latent varicella-zoster virus (VZV) in the sensory ganglia, are accompanied by rash and severe acute pain (1). About a quarter of the population suffers from HZ and associated complications throughout their lifetimes, with incidence climbing with age and/or reduced cellular immune response (2,3). Most patients with HZ completely recover after several weeks and their acute pain can be relieved by early antiviral therapies. However, some still experience chronic pain and 9-14% of patients develop postherpetic neuralgia (PHN) suffering from refractory pain sensation for months or even years after the skin lesion heals (4). This may lead to disparate secondary consequences that affect the quality of life, causing depression, withdrawal from society, and even suicide (5,6). Some evidence has proved that virus replication is not essential to induce ongoing pain, which explains the chronic pain after the rash is resolved. Therefore, it is urgent to elucidate the pathogenesis of zoster-associated pain and the development of PHN. Despite several identifications of risk factors of PHN (7) and promising novel drugs (8,9), the treatment for HN, especially PHN, remains temporary and unsatisfactory. Better understanding of HN will help to identify meaningful targets and develop new treatments.
Voltage-gated Ca 2+ channels (VGCCs) are formed by four distinct subunits (α1, α2δ, β, and γ). The α1-subunit forms a functional Ca 2+ channel and possesses the main characteristics of the channel such as ion-conducting pore, ion selectivity, and voltage sensitivity (10,11). The α2δ-subunit affects the biophysical properties and expression of the channel (12,13). The β-subunit mediates different shifts in the kinetics and voltage dependence of gating by binding to the α1-subunit (12) and the γ-subunit is involved in modulating the biophysical properties of the channel (13). According to the main biophysical and pharmacological properties, VGCCs are classified into L-, N-, P/Q-, R-, and T-type subtypes (14). Among them, T-type calcium channels are activated by weak depolarization and consist of three subtypes based on the pore-forming α1-subunit: Cav3.1 (α1G), Cav3.2 (α1H), and Cav3.3 (α1I) (15,16). Low-voltage-activated T-type calcium channels contribute to the exocytosis evoked by low voltage, serving as key regulators of neuronal excitability in the peripheral and central nervous systems (17,18). They have been reported to play a crucial role in painful pathological conditions such as complete Freund's adjuvant-or formalininduced inflammatory pain (19,20), chronic constriction injury (CCI)-or spinal nerve ligation (SNL)-induced neuropathic pain (21,22), and painful diabetic neuropathy (PDN) (23). However, there is little evidence to indicate the role of the T-type calcium channels in the zoster-associated neuralgia and development of PHN currently.
Hence, we downloaded the gene expression profile data set of the PHN model from the Gene Expression Omnibus (GEO) database and obtained preliminary evidence that calcium signals were involved in PHN by the Gene Ontology (GO) enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the differentially expressed genes (DEGs) between PHN rats and the normal control group. Next, we developed HN murine models infected with VZV to investigate the changes of T-type calcium channels in dorsal root ganglion and spinal dorsal horn after the onset of HN to confirm the important role of Cav3.2 channels in VZV-induced HN.

Culture of Cells and Viruses
Human neuroblastoma cell line (SH-SY5Y) and Human retinal pigment epithelial cell line (ARPE-19) cells were purchased from American tissue culture colection (ATCC) and cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum (Gibco, USA), 100 µg/ml streptomycin, 100 U/ml penicillin (Life Technologies, USA), and placed in 37 • C, 5% carbon dioxide (CO 2 ) atmosphere. The VZV-rOka strain was provided by Tong Cheng (Development in Infectious Diseases, Xiamen University, Xiamen, China). The preparation method of VZV virus particles is as follows: ARPE-19 monolayer cells were infected by VZV and the supernatant and cell particles were collected when 80-90% of the cells undergo cytopathic (cell rounding, swelling, and lysis) (24). The cells were disrupted by ultrasound (20 kHz, 45% amplitude, 15 s) and centrifuged at low speed (1,000 g, 5 min, 4 • C) to remove cell debris. Collect the cell lysate supernatant and concentrate the virus particles by high-speed centrifugation (80,000 g, 3 h, 4 • C). The VZV virus precipitate obtained by centrifugation was resuspended in an appropriate amount of DMEM and the virus titer was determined by the plaque test. All the virus-related experiments were carried out in the biosafety laboratory. The design and implementation of these experiments have obtained ethical and safety approval.

Animal
All the animal studies were conducted according to procedures approved by the Shenzhen Nanshan People's Hospital and the Sixth Affiliated Hospital Animal Care and Use Committee. A total of 8-week-old male C57BL/6J mice with an average body weight of 25 ± 1 g (purchased from Guangdong Medical Laboratory Animal Center: No.119, Poyang Road, Huangqi, Nanhai, Foshan, Guangdong, China) were selected. Experimental animals were housed under individually ventilated colony cages and maintained in a normal 12/12 h light/dark cycle, with free access to food and water. All the animals were operated on and cared for in accordance with the guidelines of the International Association for the Study of Pain research on the use of animals in pain research.

Preparation and Injection of Viral Inoculum
According to previous reports, the HN model was established by subcutaneous inoculation of VZV into the right hindfoot pad of mice and the injurious behaviors of mechanical hyperalgesia (MA) and thermal hyperalgesia (TH) were evaluated (25). VZV was propagated in human retinal epithelial cells  and harvested when the cells show about 80% cytopathic effect (CPE) under the microscope. The virus-infected cells were gently scraped from the surface of the culture flask and the cell suspension was centrifuged at 1,000 rpm and 4 • C for 10 min. The cell precipitates obtained from every 75 cm 2 of culture bottle were suspended in 100 µl sterile phosphate buffer solution (Gibco). The 20 µl virus-infected cell suspension (about 2 × 10 5 cells) was subcutaneously injected (SC) into the right (ipsilateral) hindfoot pad of mice. The control group was inoculated with ARPE-19 cell suspension with the same volume and cell density.

Nociceptive Behavior Test
As previously described, von Frey filaments were used to assess the mechanical injury threshold in mice (26). About 30 min prior to the test, the mice were placed in acrylic cages (12 cm × 10 cm × 17 cm) with a wire mesh floor at the bottom. In these experiments, a series of von Frey filaments with logarithmically increased stiffness (0.008-2 log force, mg) were applied vertically to the middle of the right hind paw pad in a gradually increasing pressure manner. These tests elicited reactions including flexion, leg raising, or foot licking, followed by significant withdrawal after claw withdrawal. Each von Frey filament was applied for 3-4 s to induce terminal reflex. The smallest filaments that can cause reactions were considered to be the mechanical injury threshold.
The mice were placed in the hot plate test room for 30 min to adapt to the environment in advance. The mice were placed in the acrylic cage on the heating plate (the constant temperature was 53 • C) and the latency of the first nociceptive reaction (licking the hind paw, shaking the hind paw, jumping, and spinning) was measured (27). In order to avoid tissue damage caused by high temperature, the cutoff time was 20 s.

Intrathecal Injection of Drugs
Male C57BL/J mice weighing 25 ± 1 g were selected and intrathecal drug injection was performed 7 days after VZV inoculation. The intrathecal injection method of Cav3.2 blocker 6-prenylnaringenin [(2R/S)-6-PNG] [HY-115681, MedChemExpress, Princeton, New Jersey, USA] was as follows: Hair removal on the back of mice was accomplished by a razor and the bared skin was cleaned by a cotton ball containing 75% ethanol. The pelvic girdle of mice was held safely in one hand. Then, the intrathecal injection was conducted by putting a 25-µl microsyringe with a 30-Ga needle perpendicular to the vertebrae and inserting the needle into the tissue between the dorsal sides of L5 and L6. When the needle entered the subarachnoid space, the mice tail suddenly trembled or swung laterally, indicating a successful intrathecal injection. Ten microlitre of normal saline or normal saline containing different doses (2R/S)-6-PNG (5/25/50 µg) was injected into the mouse sheath and the syringe was held for a few seconds and carefully removed to avoid drug outflow (28).

Total RNA Extraction
Dorsal root ganglia (DRG) and spinal dorsal horn (SDH) tissues were dissected from the mice: after euthanasia, the skin and muscle of the back were cut down to expose the spine and the spinous process and transverse process of the spine were gently stripped with bone cutting forceps. After exposing the SDH, the SDH was lifted with forceps to identify the enlarged DRG at the posterior root of the spinal nerve and its spinal canal. Lumbar L4-L5 DRG and ipsilateral L4-L5 SDH tissue were collected and put into a 1.5-ml centrifuge tube. Finally, the steel ball was put into the collected tissue and ground in the grinder at low temperature and then dissolved in the TRIzol reagent (Invitrogen, USA). For the cell experiment, ARPE-19 and SH-SY5Y were seeded on six-well cell plates (6 × 10 5 cells/ml). On the next day, use 1 × 10 4 Plaque forming unit (PFU)/ml acellular VZV-infected cells. After VZV infection for 24, 48, and 72 h, the cells were harvested and dissolved in the TRIzol reagent. RNA dissolved in the TRIzol reagent was extracted according to the manual of the manufacturer.

RT-qPCR for Messenger RNA Quantification
The total RNA of each sample was treated with DNase I (Thermo Fisher Scientific, USA) to remove the genome. The synthesis of complementary DNA (cDNA) from 500 ng of total RNA was reverse transcribed by using the RevertAid TM Master Mix Kit (Thermo Fisher Scientific, USA). The primer sequence was referenced from the online website of PrimerBank (https://pga.mgh.harvard.edu/primerbank/index. html) and the specificity was verified by the primer basic local alignment search tool (BLAST) tool of the National Center for Biotechnology Information (NCBI) ( Table 1). Gene expression was detected by RT-qPCR by using 2 × RealStar Green Fast Mixture [with Carboxy-X-rhodamine (ROX)] (GenStar, China) following the manual of the manufacturer. Data were collected and analyzed by using ABI QuantStudio TM version 5 software (Applied Biosystems, USA). The results were normalized to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (human)/Gapdh (mouse) and relative expression was shown as 2 − Ct .

Western Blot Analysis
Steel balls were added to the DRG and SDH collected in the centrifuge tube, adding precooled tissue lysate (Beyotime Biotechnology, China). The mixture was grounded in a precooled tissue grinder, centrifuged at 4 • C and 12,000 rpm for 10 min, and then the supernatant was collected. ARPE-19 and SH-SY5Y cells were treated with 1 × 10 4 PFU/ml cell-free VZV for 24, 48, and 72 h, respectively. The monolayer cells were scraped and centrifuged at 4 • C and 1,000 rpm for 5 min to obtain cell precipitates. An appropriate amount of precooled western cell lysate (Beyotime Biotechnology, China) was added to the cell pellet. After the cell particles were disintegrated by the lysate, the mixture was centrifuged at 4 • C and 12,000 rpm for 10 min, and the supernatant was collected. The total protein in tissue or cell lysates was quantitatively detected by Bicinchoninic acid (BCA) protein concentration assay kit (Beyotime Biotechnology, China) and the total protein was separated by 10% 12 sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein was then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, USA), which would later be blocked with 5% nonfat dry milk (Bio-Rad Laboratories, USA) dissolved in Tris buffered saline with Tween (TBS-T) for 1 h at room temperature. Different antibodies were used to detect proteins carried on different membranes and incubated at 4 • C overnight: anti-VZV glycoprotein E (gE) antibody (ab272686) (Abcam, USA; 1:2000), anti-CACNA1H antibody (ab135974) (Abcam, USA; 1:2000), anti-α-tubulin (#3873) (Cell Signaling Technology, USA; 1:2000), and anti-β-actin (#4970) (Cell Signaling Technology, USA; 1:2000). On the next day, after washing three times with TBS-T, the membrane was afterward incubated with an antirabbit or antimouse horseradish peroxidase (HRP)-conjugated antibody (CST, USA, 1:5000). Efficient chemiluminescence (ECL) Substrate Kit (Millipore, USA) was used to observe the expression of the target protein.
At least three samples were analyzed by densitometry by using the (ImageJ software, USA) and corrected for loading by normalization to corresponding bands for Gapdh.

Bioinformatics Analysis of PHN Gene Expression Profile
We downloaded the gene expression profile data set of PHN model [GSE64345 (GPL1355 platform)] from the GEO database (https://www.ncbi.nlm.nih.gov/geo/), an open-source platform for retrieving and storing gene sequencing data (29). The DEGs of DRG between PHN rats and the normal control group were screened and identified by the GEO2R online tool (https://www. ncbi.nlm.nih.gov/geo/geo2r/). The results of p < 0.05 and fold change (FC) > 1 or FC < 1 were statistically significant and were defined as DEGs. Functional classification of DEGs between groups was performed by using the DAVID (USA) version 6.8 (https://david.ncifcrf.gov/) (30) and the KOBAS (China) version 3.0 (http://kobas.cbi.pku.edu.cn/kobas3) (31) online database.

Statistical Analysis
All the quantitative data were presented as mean ± SEM from three or more independent experiments. Biochemical indicators and behavioral data were analyzed by using the one-way ANOVA or the two-way ANOVA followed by the post-hoc Bonferroni test and the statistical significance was analyzed by the GraphPad Prism version 9 (GraphPad Software, La Jolla, California, USA). When the p-value of the statistical variable is <0.05, the data were considered to be statistically significant.

Behavioral Characterization of the HN Mice
We inoculated ARPE-19 cells with VZV into the right rear footpad of male mice to create an HN model. The schematic detailed experimental process was shown in Figure 1A. The mechanical and heat thresholds were tested by using von Frey filaments and a hot plate. Mice inoculated with ARPE-19 cells with VZV showed mechanical ( Figure 1B) and thermal allodynia (Figure 1C), which peaked at 5 or 7 days after VZV inoculation. The allodynia continued until day 63 (Figures 1B,C), mimicking the chronic condition of long-lasting neuralgia that may further form PHN. VZV inoculation caused redness and swelling on the ipsilateral side, which disappeared within 7 days after virus inoculation (Figures 1D,E). This finding confirmed that it was VZV infection, rather than the inflammatory response, induced allodynia in mice.
To further confirm that the mouse inoculum is VZV, we infected the mouse inoculum in vitro with ARPE-19 and SH-SY5Y cells, which are generally considered to be induced the lytic infection by VZV. Two cell lines showed obvious cytopathic effects at 24, 48, and 72 h after infection, while the morphology of uninfected cells virus free control group (MOCK) remains normal (Supplementary Figure S1A). Moreover, the expression of the ORF61 gene (encoding the virus early phosphorylation protein) and VZV membrane gE were detected by qRT-PCR and Western blot, respectively (Supplementary Figures S1B,C). The above results suggested that the HN mice model was successfully constructed.

Varicella-Zoster Virus Infection Increased the Expression of Cav3.2 in Mice DRG and SDH
The GO and the KEGG results showed that biological events such as "calcium signaling pathway, " "calcium-mediated signaling, " and "calcium ion binding" confirmed that the calcium signal disorder was related to the occurrence of PHN (Supplementary Figures S2, S3). As an important member of the calcium channel family, low-voltage activated calcium channels, namely T-type calcium channels, are generally considered to be the key regulators of neuronal excitability in the peripheral and central nervous systems (15,17). The quantitative results of gene microarray data showed that the quantification level of Cav3.2, instead of Cav3.1 or Cav3.3 genes, was higher in the DRG of the PHN group than that of the sham group (Supplementary Figure S4).
Therefore, we tried to further clarify the dynamic changes of T-type calcium channels in the DRG and SDH from the HN mice model induced by VZV. As shown in Figure 2B, Cav3.2 mRNA in the SDH of VZV mice increased significantly compared to the sham mice on day 3 after VZV infection and the upregulation persisted until day 28 and the mRNA expression on day 28 did not return to its original level, implying that this trend may continue. Cav3.2 mRNA in DRG of VZV mice also increased significantly compared to the sham mice on days 7 and 14 after VZV infection. However, no significant difference was found in mRNA levels of Cav3.1 and Cav3.3 between the two groups, except for the slight increase of Cav3.3 in DRG on day 7 after VZV infection (Figures 2A,C). Consistently, Western blot analysis showed that the protein level of Cav3.2 increased after VZV infection in mice DRG and SDH (Figures 2D-F).
We also infected ARPE-19 and SH-SY5Y cells with VZV in vitro and conducted Western blot analysis, showing that the protein level of Cav3.2 in ARPE-19 and SH-SY5Y cells increased after VZV infection (Supplementary Figure S5D). The expression of Cav3.2 mRNA in SH-SY5Y cells also increased after VZV infection, but this trend was not detected in ARPE-19 cells (Supplementary Figure S5B), which was probably due to the low expression of Cav3.2 mRNA in ARPE-19. Interestingly, Cav3.1 mRNA was not detected in ARPE-19 and SH-SY5Y cells after VZV infection, while the expression of Cav3.3 increased in ARPE-19 cells (Supplementary Figures S5A,C).

Blocking the Cav3.2 Channel Reduces Mechanical Allodynia and Thermal Hyperalgesia in HN Mice
Given that the calcium signal disorder in the DRG and Cav3.2 was significantly upregulated in both the DRG and SDH of Frontiers in Neurology | www.frontiersin.org Representative microphotographs of the inoculated and contralateral hind paws on days 3 and 7 after VZV inoculation. (E) Comparison of paw thickness on day 3 and day 7, n = 12. Yellow arrows: ipsilateral hind paws on day 3 after VZV inoculation; blue arrows: ipsilateral hind paws on day 7 after VZV inoculation; white arrows: the contralateral hind paws; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with APPE-19 cells inactivated; #### p < 0.0001, compared with before VZV inoculation.
PHN mice, we speculated that Cav3.2 might be a key factor involved in mechanical and thermal hyperalgesia during VZVinduced neuralgia. (2R/S)-6-PNG, a Cav3.2 blocker, can potently block Cav3.2, but exhibit a minor effect on high-voltage activated Ca 2+ channels and voltage-gated Na + channels (32). VZV was inoculated into the right hind paw of the mouse to induce the HN mouse model and then (2R/S)-6-PNG was intrathecally injected on day 7 after VZV inoculation and the analgesic effect was evaluated.
Behavior tests were performed to assess mechanical and thermal sensitivity of HN mice at 30 min, 1, 3, and 24 h after intrathecal injection of (2R/S)-6-PNG (Figure 3). The schematic detailed operation process is shown in Figure 3A. Before (2R/S)-6-PNG injection, PHN mice exhibited a reduction in paw withdrawal threshold in von Frey test and hot plate test, while the increase in the threshold occurred at 1, and 3 after (2R/S)-6-PNG injection (Figures 3B,C). Collectively, these results indicated that blocking the Cav3.2 channel can alleviate neuropathic pain induced by VZV.

DISCUSSION
In clinics, there are many interventional ways to treat early HZ neuralgia such as medication, physical therapy, and surgical treatment (33). However, some patients with shingles develop PHN and their chronic pain cannot be completely cured. The fundamental reason is the complexity and uncertainty of the pathogenesis of PHN, which leads to the frustrating effect of clinical treatments for HN, especially PHN. In this study, we established a model of HN by inoculating with VZV under the footpads of mice and verified its reliability by the mechanical and thermal allodynia behavioral tests. At the same time, we measured the relative thickness of the inoculated side and contralateral footpads of the mice to rule out the allodynia caused by acute inflammation after virus inoculation, confirming that VZV infection was able to induce long-term neuropathic pain.
In order to obtain an insight into the most disturbing HN, PHN, we obtained PHN rodent DRG gene microarray data from the GEO database and used bioinformatics to explore possible pathogenesis. Compared with the control group, the results of the KEGG and the GO analysis showed that the biological functions of the DEGs in PHN rodent DRG are mainly involved in the calcium signal pathways. Calcium ion is well known as the indispensable second messenger in various physiological activities including the generation and transmission of pain-related signals between neurons (34). Although the exact molecular and cellular mechanisms of chronic pain may vary depending on the causes and types and have yet to be further elucidated, there is accumulating evidence on the role of intracellular Ca 2+ in the development of persistent pain (35). In fact, there are a variety of voltage-gated and ligand-gated Ca 2+ channels in the DRG, SDH, or glial (34,36). These calcium channels and receptors have been reported to be involved in pain and neuroplasticity associated with chronic pain states (35). In particular, our previous studies have shown that VZV infection could cause cellular calcium signal disorders manifested by significant changes in the expression of calcium signal-related genes and abnormalities in the distribution and content of calcium ions in cells (37). All the evidence implied that VZV-mediated abnormal expression and/or dysfunction of cellular calcium channels and receptors might be an important factor in the onset and maintenance of PHN.
T-type Ca 2+ channel has been reported as a key regulator of neuronal excitability in the peripheral and central nervous system, contributing to the transmission of neural signals induced by low voltage (17,38). It was first reported in peripheral sensory neurons of the DRG and subsequently confirmed to be involved in excitatory transmission in the thalamus and corticothalamic neurons (39,40). Consistently, our data showed that T-type calcium channels were expressed in mouse DRG and SDH. The time-dependent Cav3.2 expression pattern induced by VZV was surprisingly synchronized with the changes in mice pain sensitivity after VZV inoculation, showing an increasing trend first and then followed by a decrease. Intrathecal injection of Cav3.2 blocker (2R/S)-6-PNG (25 or 50 µg) significantly upregulated the reduction of mechanical and thermal hyperalgesia induced by VZV within 1-3 h. Therefore, we speculated that VZV might mediate HN by upregulating the expression of Cav3.2 and how to extend this conclusion to PHN development remains a matter of discussion.
In fact, the role of Cav3.2 in neuropathic pain has been extensively studied by behavior tests, electrophysiology, and molecular biology methods. Accompanied by enhanced T-type Ca 2+ currents, Cav3.2 expression significantly increases in the SDH and DRG neurons under the pathological conditions related to chronic pain such as peripheral nerve injury or inflammation, diabetic neuropathy, and paclitaxel-induced peripheral neuropathy (41)(42)(43)(44). In addition, blocking Cav3.2 channels by T-type calcium channel blockers, intrathecal injection of antisense oligonucleotides, or conditional gene deletion produced strong analgesic effects in rodent pain models (21,45,46). It remains elusive how the T-type Ca 2+ channels are involved in the signal transmission of pain. Given that Cav3.2 is widely expressed in the tissues including peripheral pain endings, skin afferent nerve axons, Aδ fibers, and nociceptive fibers in the spinal cord (38,46,47), we speculated that mediating the generation of instantaneous explosive potentials and altering the generation and propagation of action potentials could be the underlying mechanism of peripheral or central sensitization associated with the development and maintenance of chronic pain (48).

CONCLUSION
In this study, we established an HN model and verified that VZV inoculation of mouse footpads induced long-term mechanical and thermal pain sensitivity similar to PHN. Gene microarray data implied the involvement of calcium signal disorder in VZV-induced PHN, which was supported by our qRT-PCR and Western blotting results, confirming that VZV infection could significantly upregulate the expression level of Cav3.2 in DRG and SDH during the neuralgia. Intrathecal administration of Cav3.2 inhibitor (2R/S)-6-PNG alleviated the mechanical and thermal sensitivity of mice caused by VZV, further illustrating that Cav3.2 played a key role in zoster-associated pain and had the potential to be a novel drug target. Altogether, these findings will increase our understanding of the pathogenesis

DATA AVAILABILITY STATEMENT
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author/s.

ETHICS STATEMENT
The animal study was reviewed and approved by Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center Animal Care and Use Committee.

AUTHOR CONTRIBUTIONS
SW, DX, and LX contributed to the study supervision and coordination and the design of the experiments. SW and