Impact Factor 3.648 | CiteScore 3.99
More on impact ›

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Neurosci. | doi: 10.3389/fnins.2019.01264

MiR-409-5p as a regulator of neurite growth is down regulated in APP/PS1 murine model of Alzheimer's disease

 Jun Wan1*,  Jing Guo1,  Yifei Cai1, Xiaoyang Ye1,  Nana Ma1,  Yuan Wang1 and Bo Yu2
  • 1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University Hong Kong University of Science and Technology Medical Center, China
  • 2Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University Hong Kong University of Science and Technology Medical Center, China

Alzheimer’s disease (AD) is a heterogeneous neurodegenerative disease. Recent studies suggest that miRNAs expression changes are associated with the development of AD. Our previous study showed that the expression level of miR-409-5p was stably down-regulated in the early stage of APP/PS1 double transgenic mice model of AD. We now report that miR-409-5p impairs neurite outgrowth, decreases neuronal viability and accelerates the progression of Aβ1-42-induced pathologies. In this study, we found that Aβ1-42 peptide significantly decreased the expression of miR-409-5p, which was consistent with the expression profile of miR-409-5p in the APP/PS1 mice cortexes. PLEK was confirmed to be a potential regulatory target of miR-409-5p by luciferase assay and Western blotting. Overexpression of miR-409-5p has an obvious neurotoxicity in neuronal cell viability and differentiation, whereas PLEK overexpression could partially rescue neurite outgrowth from this toxicity. Some cytoskeleton regulatory proteins have been found to be related to AD pathogenesis. Our data show some clues that cytoskeletal reorganization may play roles in AD pathology. The early down-regulation of miR-409-5p in AD progression might be a self-protective reaction to alleviate the synaptic damage induced by Aβ, which may be used as a potential early biomarker of AD.

Keywords: Alzheimer ' s disease, Beta amyloid (Aβ), microRNA, miR-409-5p, Plek

Received: 20 Aug 2019; Accepted: 07 Nov 2019.

Copyright: © 2019 Wan, Guo, Cai, Ye, Ma, Wang and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Jun Wan, Shenzhen Peking University Hong Kong University of Science and Technology Medical Center, Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen, 518000, China, wanj@ust.hk