%A Hassan,Abdul-Azim %A Sleet,Ben %A Cousins,Zoe %A Keating,Chris David %D 2020 %J Frontiers in Neuroscience %C %F %G English %K TRPA1,motility,Colon,HNE (4-hydroxy-2-nonenol),Enteric Nervous System,Transient receptor channels %Q %R 10.3389/fnins.2020.00471 %W %L %M %P %7 %8 2020-May-27 %9 Original Research %# %! TRPA1 Activation Inhibits Colon Motility %* %< %T TRPA1 Channel Activation Inhibits Motor Activity in the Mouse Colon %U https://www.frontiersin.org/articles/10.3389/fnins.2020.00471 %V 14 %0 JOURNAL ARTICLE %@ 1662-453X %X There is a growing awareness of the role that TRP channels play in regulating sensory and motor functions in the gastrointestinal tract. In this study we used an in-vitro murine model of colonic peristaltic-like complexes (CPMCs) to evaluate the role of exogenous and endogenous TRPA1 signaling processes in regulating colonic motility. Using in-vitro recordings of intraluminal pressure to monitor the presence of CPMCs in colonic segments we performed a series of experiments on male CD1 mice (2 months of age) and found that CPMC activity was attenuated by TRPA1 agonists. Bath application of the TRPA1 antagonist HC-030031 had no effect upon basal CPMC activity whereas application of the synthetic TRPA1 agonist ASP7663 caused a reversible dose dependent decrease in CPMC frequency that was blocked by HC-030031. Cinnamaldehyde and 4-hydroxy-2-nonenal elicited long lasting decreases in CPMC frequency that were blocked by HC-030031 whereas the decreased CPMC activity invoked by AITC could not be blocked by HC-030031. Our results show that any potential mechanosensory function of TRPA1 doesn’t involve contributing to distension induced colonic motor activity and that a role for TRPA1 in the colon is through regulating motility through exogenous and endogenous agonist induced inhibitory effects.