Mentha rotundifolia (L.) Huds. aqueous extract attenuates H2O2 induced oxidative stress and neurotoxicity

Introduction Oxidative stress plays a causal role in neurodegenerative diseases. The aim of this study is to evaluate the antioxidant and neuroprotective effects of Mentha rotundifolia (L.) Huds (M. rotundifolia), a widely used Moroccan plant in traditional medicine. Methods The chemical composition of M. rotundifolia aqueous extract was analyzed by liquid chromatography coupled to mass spectrometry (LC-MS). 2,2-diphenyl 1-picrylhydrazyl (DPPH) and 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) assays were used to assess its in vitro antioxidant activity. H2O2 was utilized to induce oxidative stress and neurotoxicity in vivo. Behavioral changes were evaluated using Open Field, Y-maze and Rotarod tests. Hyperalgesia was assessed using the tail immersion test. Results and discussion The LC-MS/MS analysis revealed high content of kaempferol glucuronide (85%) at the extract. IC50 values of the DPPH and ABTS were 26.47 and 41.21 μg/mL, respectively. Pre-treatments with M. rotundifolia extract attenuated the behavioral changes induced by H2O2. In addition, the latency of tail withdrawal increased significantly in the treated groups suggesting central analgesic effect of M. rotundifolia extract. Moreover, the extract attenuated the deleterious effects of H2O2 and improved all liver biomarkers. The obtained results suggested that M. rotundifolia had remarkable antioxidant and neuroprotective effects and may prevent oxidative stress related disorders.


Introduction
The mitochondrial electron transport chain plays a key role in the cell's energy production via a series of electron transfer reactions (Rich and Maréchal, 2010). Under physiological conditions, up to 4% of electrons may escape and react directly with dissolved oxygen in the cytoplasm, giving rise to reactive oxygen species (ROS). The latter are either 2. Materials and methods

Plant material and extraction
Mentha rotundifolia (L.) Huds. leaves were collected at the Mkam Tolba village, located near Khemisset city (33 • 55 41.9 N, 6 • 16 34.2 W), Morocco, during the flowering period (August 2022). The leaves were dried in shade for 2 weeks at room temperature. The dried leaves were ground, and a 100 g was infused in hot distilled water (1000 mL × 90 • C) for 15 min. The infusion was then filtered, reduced under vacuum, and lyophilized yielding a crude extract (13.43 ± 1.04%, n = 3).

Chemical composition characterization
A Shimadzu system coupled to an MS 8050 triple quadrupole mass spectrometer was utilized. Separation was performed using a C18 reversed phase column (Zorbax Eclipse XDB-C18, 4.6 mm × 150 mm, 3.5 µm, Agilent, USA). A gradient of water and acetonitrile (ACN) (0.1% formic acid each) was applied from 5 to 30% ACN over 45 min and then increased to 58% ACN for the next 15 min with a flow rate of 1 mL/min. The sample was automatically injected using an autosampler SIL-40C XS autosampler. The instrument was controlled by LabSolutions software. Ions were detected in the negative ion mode, a full scan mode and a mass range of 100-1500 m/z (Tawfeek et al., 2023).

In vitro antioxidant activity 2.3.1. DPPH assay
The free radical scavenging activity of M. rotundifolia aqueous extract was tested using 2,2-diphenyl-1-picryl-hydrazyl (DPPH.), taking ascorbic acid as reference standard. A volume of 0.5 mL of DPPH (0.2 mM) was added to the extract at different concentrations ranging from 10 to 100 µg/mL. The reaction was then incubated for 30 min in the dark at room temperature (Boualam et al., 2021). The absorbance was read at 517 nm, and the scavenging activity was estimated using the following equation line: The experiment was performed three times.

ABTS assay
The test is based on the ability of an antioxidant to stabilize the blue-green colored cationic radical ABTS. + (2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate) by trapping a proton and transforming it into colorless ABTS + . ABTS + solution (1.9 mL) has been added to 600 µL of extract solution at different concentrations (10-1000 µg/mL). After incubation for 7 min at room temperature, the absorbance was measured at 734 nm (Boualam et al., 2021). The Experimental design of the in vivo study.
blank was prepared by replacing the extract solution with methanol. The scavenging activity was estimated using the following equation: The experiment was performed three times.

In vivo antioxidant activity 2.4.1. Animals and experimental design
The experiments were performed on Wistar rats weighing 180-200 g and raised at the central animal care facilities of the Faculty of Sciences, Mohammed V University of Rabat, Morocco. Animals were randomly housed in polyethylene cages with a free access to food and water in a room with controlled temperature (22 ± 1 • C) and under a 12 h light-dark cycle. The animals were divided randomly to the following groups with their respective treatment: -Group 1 and 2 received 1 mL saline intraperitoneally (i.p) for 21 days. -Group 3 and 4 received 62.5 mg/kg M. rotundifolia extract (i.p) for 21 days. -Group 5 and 6 received 125 mg/kg M. rotundifolia extract (i.p) for 21 days. -Group 7 and 8 received 250 mg/kg M. rotundifolia extract (i.p) for 21 days. At day 8, 2% H 2 O 2 was added to the drinking water of groups 2, 4, 6, and 8, for the remaining 15 days of treatment as described at Figure 1 (Bakour et al., 2018;Bouayed and Soulimani, 2019). During the treatment period, the animals were weighed every 5 days to highlight the effect of H 2 O 2 on the body weight (BW) and to evaluate the potential protective effect of M. rotundifolia aqueous extract against H 2 O 2 toxicity.

Behavioral tests 2.4.2.1. Open field test
The open field test (OF) is a common measure of exploratory behavior and general locomotor activity in mice and rats (Gould et al., 2009). It's also used to assess the animal's anxiety behavior through parameters such as the number of defecations or areas visited (Seibenhener and Wooten, 2015). The OF device used in this study is a square black device with a surface area of 80 cm 2 , with a height of 45 cm. The ground of the field is divided with white strips into 25 squares of 15 cm × 15 cm each, Supplementary Figure 1. This test is performed in a dimly lit room where the animal is placed at the center. The field is cleaned after each test. Each animal spent 10 min in the OF. Locomotor activity is evaluated by the distance covered by the animal (the number of tiles crossed) and the number of sit-ups performed. Anxiety-Like symptoms were assessed by the number of entries in the central zone of the OF and the time spent in this zone.

Rotarod test
The rotarod test is widely used to evaluate the motor coordination in rodents (Shiotsuki et al., 2010). The apparatus is made of a plastic rotating cylinder, 7 cm in diameter and 28 cm long, fixed around a horizontal axis raised by 30 cm and divided into 4 compartments of 9 cm width each, Supplementary Figure 2 (Panlab, Harvard Apparatus). The test is performed in an isolated room with normal lighting and programmed over two phases: The pre-test phase where the animals undergo habituation and the test phase where the rats are placed on the rotating cylinder at a fixed speed of 4 rotations per min (rpm). Once the animal is stable, the apparatus is adjusted to increase the speed of rotation until a maximum speed of 40 rpm is reached, which is maintained fixed until the end of the test. The time spent on the cylinder does not exceed 180 s.

Y-maze test
The Y-maze test can be used to assess both spatial working and spatial reference memories in rodents (Kraeuter et al., 2019). It's a black Y-shaped device with three arms orientated at 120 • angles from each other, Supplementary Figure 3. For the spatial working memory, the spontaneous alternation is calculated. For this, the number of arm entries and alternations (consecutive entries into all three arms) are recorded to calculate the percentage of the alternation behavior defined by the following formula (Kraeuter et al., 2019): % Alternation = (Number of Alternations / Total number of arm entries − 2 ) × 100 As for reference spatial memory, it is organized in two phases: the training session, when the animal is placed in the Y-maze device Frontiers in Neuroscience 03 frontiersin.org with one arm closed off and determined as the novel arm. This session is followed by the test session, where the animal is placed back in the device after a 1 h time interval. The animal should remember which arm was not explored previously and should visit this novel arm more frequently than the others. The number of entries into the novel arm is then compared to the entries into the other arms to assess the degree of spatial memory.

Tail immersion test for hyperalgesia
Hyperalgesia is defined as a state of enhanced intensity of pain sensation induced by either noxious or ordinarily nonnoxious stimulation of peripheral tissues (Taïwe and Kuete, 2017). Hyperalgesia was assessed using tail immersion test described by Janssen et al. (1963). Briefly, the animals were placed in the restraint cylinder, and the tail tip was submerged in a water bath at 50 ± 0.5 • C. A flick of the tail away from the heat source is produced as a nociceptive reflex is spinally mediated (Bannon and Malmberg, 2007). The latency of the tail withdrawal reflex was measured. Each immersion was terminated after 15 s to minimize potential skin damage.

Determination of hepatic parameters
All animals were sacrificed by decapitation 24 h after the last test and overnight fasting. Blood samples were collected, and the serum was separated for assaying the liver biomarkers. The serum levels of ALT, AST and total bilirubin were assessed according to the standard methods described in Garber (1981) and Huang et al. (2006).

Statistical analysis
Data were analyzed using GraphPad Prism 8. Normality was assessed by the Shapiro-Wilk test. IC 50 values were presented as means ± SD (n = 3) and were compared using t-test. In vivo test results were analyzed using two-ways ANOVA considering the treatment and exposure to H 2 O 2 as variation factors. Bonferroni's multiple comparisons test was used whenever there was significance. Results are expressed as means ± SD (n = 6). The differences were regarded as statistically significant at p ≤ 0.05.

Chemical composition
Liquid chromatography analysis of M. rotundifolia leaves aqueous extract revealed forty phytoconstituents mainly phenolic acids, flavonoids, and their glucosides (Table 1 and Figure 2). Kaempferol glucuronide dominated the extract with a relative abundance of 85%.

In vitro antioxidant activity
Mentha rotundifolia aqueous extract furnished solid antioxidant activities in DPPH and ABTS assays (IC 50 ≤ 50 µg/ml) in a concentration-dependent manner ( Table 2 and Supplementary Figures 4A, B). The obtained results might be attributed to the high contents of polyphenolic compounds, among them phenolic acids and flavonoids. Liquid chromatography profile of Mentha rotundifolia leaves aqueous extract.

Body weight monitoring
Animals were weighed each 5 days during the test period to highlight the effect of oxidative stress on the body weight (BW) and to evaluate the potential protective effect of M. rotundifolia aqueous extract against H 2 O 2 -induced toxicity. As awaited, H 2 O 2 intake significantly decreased the body weight of animals (Group 2) compared to the control group (Group 1) starting from the day 15th of the treatment, Figures 3A, B. Interestingly, M. rotundifolia extract at two tested doses (125 and 250 mg/kg) attenuated these effects starting from the 10th day of treatment. Noteworthy, there was no significant differences between the treated groups with the extract only (Groups 3, 5, and 7) and the control group (Group 1) at the end of the experiment, Figures 3A

. Locomotion
The locomotion of animals was assessed using the OF test. For this, the total distance crossed by the animal and the number of situps (when the animal stands on its two hind paws) were calculated. As expected, H 2 O 2 intake remarkably reduced the number of crossed squares of animals (Group 2) compared to control group (Group 1), p < 0.0001, Figure 4A. M. rotundifolia extract at the two tested doses (62.5 and 125 mg/kg) reversed the effects of H 2 O 2 , Figure 4A. Again, H 2 O 2 intake notably declined the number of sit-ups (number of times the animals stood on their hind paws) of animals (Group 2) compared to control group (Group 1), p < 0.0001, Figure 4B. Noticeably, M. rotundifolia extract at the three tested doses (62.5, 125, and 250 mg/kg) ameliorated the effects of H 2 O 2 with no significant differences between the three doses, Figure 4B. Noteworthy to highlight, the numbers of crossed squares and sit-ups were significantly increased in the extract treated groups (p ≤ 0.05) in normal rats (Tap water) ,  Figures 4 A, B.

Anxiety-like behavior
The anxiety-like behavior of animals was evaluated using the OF test, where the number of entries and the time spent in the inner zone were calculated. Agreeing with the above assays, H 2 O 2 intake significantly (p ≤ 0.0001) decreased the number of entries to the inner zone of the groups 2, 4, and 8, Figure 5A. These effects were inverted in 125 mg/kg M. rotundifolia extract treated group which has a comparable number of entries than control group (group 1), Figure 5A. Again, H 2 O 2 intake significantly decreased the time spent in the inner zone compared to the control group (Group 1), Figure 5B. Animals treated with M. rotundifolia (125 and 250 mg/kg doses) spent significantly more time in the inner zone compared to H 2 O 2 group (Group 2) and their times were comparable to the control group (Group 1), Figure 5B. There were no significant differences between the extract treated groups (Groups 3, 5, and 7) and control group (Group 1) for both parameters.

Rotarod test
Oxidative stress causes motor coordination and impairment. The protective potential of M. rotundifolia aqueous extract against such deleterious effect was evaluated using the rotarod test. H 2 O 2 intake significantly decreased the motor coordination in animals (Group 2) compared to control group (Group 1), (p ≤ 0.05). Results were presented as mean ± SD. Comparison was made to the corresponding reference compounds using t-test. **p ≤ 0.01. ****p ≤ 0.0001.

Y-maze test
The Y-maze test was used to assess the effect of oxidative stress on short-term memory and the protective effect of M. rotundifolia aqueous extract against memory impairment. The spatial working memory was evaluated by the measure of the spontaneous alternance, Figure 7A. Alternance was significantly (p ≤ 0.05) decreased in the H 2 O 2 only administered group (group 2) compared to saline group (group 1). This effect was reversed by M. rotundifolia extract at 125 mg/kg, Figure 7A. The spatial reference memory was evaluated by calculating the number of entries in the novel arm, Figure 7B. H 2 O 2 intake induced a significant reduction (p ≤ 0.05) in the number of entries in the novel arm in the groups (2, 4, and 8) Effect of the interaction between the extract treatment and the exposure to H 2 O 2 on locomotion assessed by the OF test. (A) The distance crossed by animals defined by the number of squares. (B) The number of animals' sit-ups. The results were presented as mean ± SD (n = 6). Data were analyzed by Two-ways ANOVA followed by Bonferroni's post-hoc test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 compared to saline group in the same treatment conditions. (#) in comparison with the control group (group 1).
in comparison with control group (group 1). This effect was reverted in 125 mg/kg extract treated group (Group 6), which had no significant difference (p > 0.99) with the control group (group 1).

Tail flick test for hyperalgesia
Tail flick test was used to assess the effect of H 2 O 2 exposure and treatment by the extract on pain perception. For this, the tail withdrawal reflex latency was recorded, Figure 8. H 2 O 2 intake remarkably decreased (p ≤ 0.0001) the response time compared to the control group (Group 1). Pre-treatment with M. rotundifolia extract attenuated these effects at two tested doses (125 and 250 mg/kg). Noticeably, M. rotundifolia treated groups, at the three tested doses, had a significant (p ≤ 0.05) higher latency time independently from the oxidative stress, which demonstrates the analgesic effect of M. rotundifolia.

Hepatic biomarkers concentrations
Hepatic enzymes were assayed as shown in Table 3. H 2 O 2 intake markedly elevated the three tested biomarkers (ALT, AST, Frontiers in Neuroscience 06 frontiersin.org The results were presented as mean ± SD (n = 6). Data were analyzed by Two-ways ANOVA followed by Bonferroni's post-hoc test; ***p ≤ 0.001, ****p ≤ 0.0001 compared to saline group in the same treatment conditions. (#) in comparison with the control group (group 1). and total bilirubin) compared to the control group (Group 1). M. rotundifolia extract decreased the elevated levels of ALT and AST at the two tested doses 125 and 250 mg/kg and bilirubin at the three tested doses. Interestingly, M. rotundifolia at the dose of 250 mg/kg induced a significant mild elevation in the total bilirubin level (p ≤ 0.05) in comparison to control group (group 1). Additionally, there was no significant difference (p > 0.05) between the groups receiving this dose in "Tap water" (group 7) and those receiving H 2 O 2 (group 8).

Discussion
H 2 O 2 is generated by several sources of oxidative stress. It's considered as a signaling biological messenger due to its ability to diffuse freely into many types of tissues and membranes (Gammella et al., 2016). It damages important classes of biological macromolecules in cells directly by oxidizing lipids, proteins, and Effect of the interaction between the extract treatment and the exposure to H 2 O 2 on motor coordination assessed by the rotarod test. The time spent on the cylinder was calculated. The results were presented as mean ± SD (n = 6). Data were analyzed by Two-ways ANOVA followed by Bonferroni's post-hoc test; ***p ≤ 0.001, ****p ≤ 0.0001 compared to saline group in the same treatment conditions. (#) in comparison with the control group (group 1).
nucleic acids, which result in many diseases (Guesmi et al., 2018). The cells' antioxidant defenses counteract H 2 O 2 and ROS that have crossed the cell membrane. However, once they reach a threshold, cells eventually succumb to severe oxidative damage, leading to cell death (Law et al., 2014). Oxidative stress has been also linked to cognitive decline in several medical conditions, including aging, traumatic brain injury, and Alzheimer's disease. In fact, most studies on cognitive impairment in Alzheimer's disease link it to oxidative stress through decreased levels of antioxidant enzymes (Alzoubi et al., 2012).
In this context, the present study was conducted to evaluate the protective effect of the aqueous extract of M. rotundifolia leaves against H 2 O 2 induced toxicity. H 2 O 2 exposure significantly decreased the locomotion activity evaluated by the OF and Rotarod tests as it was shown with other substances inducing oxidative stress such as aspartame, clomipramine, and paraquat (Niveditha et al., 2017;Onaolapo et al., 2017;Othman et al., 2019). It also induced Anxiety-like symptoms defined by the number of entries and the time spent in the inner zone of the OF. In the Y-maze test, the spatial working memory and the spatial reference memory were represented by the spontaneous alternance and the number of entries in the novel arm, respectively, which was significantly decreased due to H 2 O 2 exposure. As awaited, the plant treated groups showed less oxidative stress related behavior impairments especially at the doses of 125 mg/kg. These findings may be attributed to the high content of neuroprotective compounds in M. rotundifolia extract. It was shown that kaempferol, identified in the extract, act as neuroprotective agent against rotenone-induced Parkinson's disease model of rats by preventing the loss of tyrosine hydroxylase expression (Pan et al., 2020). In addition, its administration protected against Chlorpyrifos-induced oxidative stress and memory deficits in rats via GSK3β-Nrf2 signaling pathway and increased the activities of antioxidant enzymes and AChE (Hussein et al., 2018). Furthermore, the intracerebroventricular Effect of the interaction between the extract treatment and the exposure to H 2 O 2 on short-term memory assessed by the Y-maze test. (A) Presents the percentage of alternance as an indicator of the spatial working memory. (B) Presents the number of entries in the novel arm as an indicator of spatial reference memory. The results were presented as mean ± SD (n = 6). Data were analyzed by Two-ways ANOVA followed by Bonferroni's post-hoc test; *p ≤ 0.05 compared to saline group in the same treatment conditions. (#) in comparison with the control group (group 1).
Micro-injection of Kaempferol reduced anxiety through the GABAergic mechanism by binding to specific sites of benzodiazepines (Zarei et al., 2021). However, in H 2 O 2 conditions, it was observed that the group received a 250 mg/kg dose of M. rotundifolia extract had significantly less locomotor activity. It was thought that this was related to the toxicity or the sedative effect of the extract, but according to the literature, the plant is safe even at high doses and has no psychotropic effect (Bounihi, 2016). Further investigations should be carried out to explain these results.
Hyperalgesia was assessed using the tail immersion test. H 2 O 2 exposure significantly decreased the latency of the tail reflex. Several studies have related hyperalgesia to oxidative stress and showed that it can be attenuated by antioxidant treatments (Mohajjel Nayebi et al., 2021). The extract treated groups, whether in "Tap water" or "H 2 O 2 " conditions showed dose-dependent responses. Tail withdrawal latency Effect of the interaction between the extract treatment and the exposure to H 2 O 2 on hyperalgesia evaluated by the tail immersion test. The latency of the tail withdrawal reflex was measured. The results were presented as mean ± SD (n = 6). Data were analyzed by Two-ways ANOVA followed by Bonferroni's post-hoc test; **p ≤ 0.01, ****p ≤ 0.0001 compared to saline group in the same treatment conditions. (#) in comparison with the control group (group 1).
was maximal at 250 mg/kg suggesting an analgesic effect of M. rotundifolia extract. It was previously shown that at a dose of 600 mg/kg, M. rotundifolia extract had a greater effect than aspirin on acetic acid induced writhing in mice (Boussouf et al., 2017).
The liver is involved in the detoxification and elimination of xenobiotics, some of which are potentially toxic. This leads to oxidative damage of hepatocytes, reflected by the increase of hepatic enzymes. Additionally, liver plays a key role in the metabolism of orally and intra-peritoneally administered drugs (Turner et al., 2011), the two routes we chose for the administration of H 2 O 2 and M. rotundifolia extract, respectively. It was even shown that impaired liver function was predisposing factor for encephalopathy and neurodegenerative diseases (Butterworth, 2013). Furthermore, in a cohort study of 1,581 older adults, elevated AST and ALT levels were associated with the diagnosis of Alzheimer's disease, poor cognition, and reduced glucose metabolism in the brain (Nho et al., 2019). Thus, substances able to regulate liver biomarkers may be effective in preventing neurodegenerative diseases. The major antioxidant enzymes that can eliminate H 2 O 2 are catalase, glutathione peroxidase and peroxiredoxins (Phaniendra et al., 2015). It was previously demonstrated that M. rotundifolia extract significantly improved the activities of these enzymes in the liver homogenate after carrageenan injection (Mohajjel Nayebi et al., 2021). Hepatic biomarkers (ALT, AST, and Bilirubin) were assayed as well in this work. The end products of some metabolic pathways such as uric acid, taurine, and bilirubin can have cytoprotective effects, but at the same time, they are harmful at high levels (Glazer, 1989). In the present study, we focused on the antioxidant effect of bilirubin. H 2 O 2 exposure induced a significant hyperbilirubinemia accompanied by a significant increase in AST and ALT levels. M. rotundifolia extract significantly decreased the elevated levels in a dose-dependent manner. At a dose of 250 mg/kg, AST and ALT Results were presented as mean ± SD (n = 6). Data were analyzed by Two-ways ANOVA followed by Bonferroni's post-hoc test. a,b Significantly different from the Tap water group (Group 1) and H2O2 group (Group 2) at p ≤ 0.05. levels in H 2 O 2 groups were comparable to those in "Tap water" groups.
In general, the characterized phytoconstituents have been already reported in prior works for their pharmacological properties. Quinic and chlorogenic acids have a wide range of biological activities such as antidiabetic, DNA protective, neuroprotective, hepatoprotective, and antifungal effects (Ma and Ma, 2015;Zuo et al., 2015). Caffeoyl shikimic acid showed an anti-hyperuricemia effect and ameliorated kidney injury in mice . Kaempferol and its derivatives are flavonols with promising bioactivities including anti-cancer, antiviral, antimicrobial, and neuroprotective properties (Bangar et al., 2022). Additionally, all these compounds have been shown to possess an important antioxidant activity (Sato et al., 2011;Uzun et al., 2017;Riahi et al., 2019), which may explain the strong antioxidant potential of M. rotundifolia aqueous extract in DPPH and ABTS assays, as it was previously confirmed (Fatiha et al., 2015;Siham et al., 2019).
Noteworthy, when bilirubin acts as an antioxidant, it can protect the cells against up to 10,000 times higher concentrations of H 2 O 2 (Doré et al., 1999). In the current work, administration of M. rotundifolia extract resulted in a mild elevation in bilirubin level. This may be due to the richness of M. rotundifolia in flavonoids as it was previously demonstrated with isolated flavonoids from of the Milk thistle, Silybum marianum L., induced elevated bilirubin concentrations (Šuk et al., 2019). Patients with Gilbert's syndrome, which is characterized by mild elevated bilirubin, have been shown to have a significant lower level of oxidative stress without experiencing symptoms of toxicity related to elevated bilirubin levels (Maruhashi et al., 2012). Furthermore, it was found that the induction of mild hyperbilirubinemia may have an important therapeutic potential specially to prevent diseases related to oxidative stress such as cardiovascular disease, type 2 diabetes, metabolic syndrome, and neurodegenerative disorders (Vitek et al., 2019). These findings suggest that the M. rotundifolia leaves have an important antioxidant potential, and their phytoconstituents may be good candidates to develop new drugs for oxidative stress comorbidities.
Substantial differences were found at the chemical composition of M. rotundifolia from the Moroccan flora compared to those from other countries. In the current work, kaempferol glucuronide (accounted for 85%) dominated the extract along with other minor compounds, among them quinic acid, chlorogenic acid, caffeoyl shikimic acid, and kaempferol rutinoside. Noteworthy, rosmarinic acid was detected at the extract as a minor compound; however, it was among the major compounds from the Algerian and Tunisian species (Fatiha et al., 2015;Siham et al., 2019). While eriocitrin was predominant in Saudi Arabian flora (Alharbi et al., 2021). These variations may be explained by several factors such as geographical distribution, temperature, water availability and soil composition (Kabtni et al., 2020).

Conclusion
Mentha rotundifolia L. is traditionally used for its analgesic, antispasmodic, and anti-inflammatory properties. The current study is the first of its kind carried out for this plant in terms of its neuroprotective potential in an in vivo model. M. rotundifolia extract furnished in vitro antioxidant activities, reduced the effects of H 2 O 2 -induced oxidative stress, and attenuated the associated behavioral changes. These activities may explain its traditional uses in health and nutrition supplements and promotes its valorization as a nutraceutical. However, further analysis should be performed for better understanding of its neuroprotective effect pathway, isolate its individual components, and determine its therapeutic dose, onset and duration of action.

Data availability statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement
All animal procedures were conducted in accordance with international ethical standards (European Union Directive 2010/63/EU) legislation and ARRIVE (Animal Research Reporting of In Vivo Experiments) guidelines.

Author contributions
KB, NB, and MS designed the study, analyzed the data, and contributed to the writing of the manuscript. MT and KT supervised and provided critical feedback that shaped the final version of the manuscript. All authors contributed to the article and approved the submitted version.