The nuclear events guiding successful nerve regeneration

Peripheral nervous system (PNS) neurons survive and regenerate after nerve injury, whereas central nervous system (CNS) neurons lack the capacity to do so. The inability of the CNS to regenerate presumably results from a lack of intrinsic growth activity and a permissive environment. To achieve CNS regeneration, we can learn from successful nerve regeneration in the PNS. Neurons in the PNS elicit dynamic changes in gene expression in response to permissive environmental cues following nerve injury. To switch gene expression on and off in injured neurons, transcription factors and their networks should be carefully orchestrated according to the regeneration program. This is the so-called “intrinsic power of axonal growth.” There is an increasing repertoire of candidate transcription factors induced by nerve injury. Some of them potentiate the survival and axonal regeneration of damaged neurons in vivo; however, our knowledge of transcriptional events in injured neurons is still limited. How do these transcription factors communicate with each other? How does the transcriptional machinery regulate the wide variety of regeneration-associated genes (RAGs) in the properly coordinated manner? In this review, we describe our current understanding of the injury-inducible transcriptional factors that enhance the intrinsic growth capacity, and propose a potential role for specificity protein 1 (Sp1), which provides a platform to recruit injury-inducible transcription factors, in simultaneous gene regulation. Finally, we discuss an additional mechanism that is involved in epigenetic modifications in damaged neurons. A comprehensive understanding of the nuclear events in injured neurons will provide clues to clinical interventions for successful nerve regeneration.


INTRODUCTION
Unlike neurons of the peripheral nervous system (PNS), the central nervous system (CNS) neurons are unable to survive and regenerate after nerve injury (Filbin, 2003;Harel and Strittmatter, 2006). This serious problem has led to many studies being focused on how to drive axon elongation in the CNS. However, the molecular mechanisms underlying successful nerve regeneration in the PNS remains to be elucidated. It will be difficult to overcome the problem in the CNS without a basic understanding of the exact mechanisms underlying successful nerve regeneration. From this respect, an understanding of nerve regeneration in the PNS is of both basic and potential clinical interest.
Recent large-scale screening approaches using a combination of microarrays with phosphoproteomics have identified 39 injury-inducible transcription factors (Michaelevski et al., 2010), while the comparative transcriptome microarray analysis of peripheral versus central nerve injured models of dorsal root ganglion (DRG) neurons found 30 candidate transcription factors (Stam et al., 2007). These injury-inducible transcription factors are presumed to control hundreds of transcriptional targets. It has been established that the 5 -flanking sequences of some RAGs such as an important regulator of growth cone motility, GAP43 (Kobayashi et al., 1994), intermediate filament, peripherin (Terao et al., 2000), and cytoskeletal protein, α-tubulin (Tetzlaff et al., 1988), have injury-responsive characteristics (Belecky-Adams et al., 1993;Gloster et al., 1994;Vanselow et al., 1994;Uveges et al., 2002). Recent genome-wide screening of gene promoters identified multiple potential target genes (MacGillavry et al., 2011). However, our knowledge of the targets is limited.
A principal goal in the field has been to identify the master regulators that coordinate the regenerative program, and to understand the global gene regulation processes that drive intrinsic growth ability. It is required to know how such a large number of transcription factors interplay with each other to regulate RAGs synchronously, and how global and dynamic changes in gene expressions are controlled. In this review, we describe the current understanding of the transcription factors associated with nerve regeneration and highlight our finding of the specificity protein 1 (Sp1)-dependent transcriptional machinery as a result of its interactions with injury-inducible transcription factors. This finding has raised several unanswered questions to be addressed in the future. Finally, we discuss the possible involvement of epigenetic regulation in nerve regeneration, including chromatin remodeling and DNA methylation.

TRANSCRIPTION FACTORS INVOLVED IN NERVE REGENERATION
The physiological relevance of both injury-inducible transcription factors and their potential targets awaits in vivo study. Recent exciting work using knockout and transgenic mice is uncovering their relevance, as shown in Table 1. C/EBPβ knockout mice showed reduced expression of RAGs such as α-tubulin and GAP-43 after facial nerve injury (Nadeau et al., 2005). Deletion of the tumor suppressor p53 rescued the motor neuron death after hypoglossal nerve injury by preventing expression of the proapoptotic gene Noxa (Kiryu-Seo et al., 2005). However, Giovanni et al. demonstrated that the role of p53 is predominantly one of axonal regeneration rather than cell survival after facial nerve axotomy (Di Giovanni et al., 2006). It is possible that p53 has a dual role, because p53 is a multifunctional and stress-sensitive protein that initiates numerous transcriptional cascades in response to nerve injury. The absence of Krüppel-like factor-4 (KLF4) in retinal ganglion cells modestly enhanced axonal regeneration after optic nerve injury, suggesting that KLF4 is a negative regulator of intrinsic growth ability (Moore et al., 2009). Gao et al. have shown that the injection of the adenovirus containing constitutively active CREB into the DRG enhanced nerve regeneration, although these authors did not use a genetically modified mouse (Gao et al., 2004). All of these studies have provided valuable in vivo information about the gene regulation events that elevate intrinsic growth ability. Furthermore, the prominent effects of c-Jun, ATF3, and STAT3 on nerve regeneration have been particularly well examined. Here, we summarize the current understanding of the roles of these three injury-inducible transcription factors in nerve regeneration.

C-JUN
c-Jun is a major component of the activator protein-1 (AP-1) family of transcription factors and forms homo-and heterodimers together with Jun family proteins (c-Jun, JunB, and JunD) and Fos proteins (c-fos, FosB, Fra-1, and Fra-2) (Herdegen and Leah, 1998). Many Jun family proteins depend on the activation of N-terminal kinases (JNKs) and are involved in a large number of cellular functions. N-terminal phosphorylated c-Jun is rapidly accumulated in the nucleus where it promotes neuronal death (Herdegen et al., 1997). This phenomenon is observed during embryonic development as well as in a variety of nerve injury models (Herdegen et al., 1997;Raivich et al., 2004;Lindwall and Kanje, 2005). Thus, overexpression of c-Jun leads to cell death by inducing propapoptotic genes such as Bim, whereas c-Jun suppression or dominant-negative c-Jun expression prevents neuronal death (Ham et al., 1995;Whitfield et al., 2001;Palmada et al., 2002). Peripheral nerve injury induces c-Jun expression, suggesting its involvement in nerve regeneration (Raivich et al., 2004). However, the situation is not so simple. Neuronal death is sometimes induced even by peripheral nerve axotomy. This phenomenon is more augmented in mice than in rats (Kiryu-Seo et al., 2005. Using this feature, Raivich et al. demonstrated that c-Jun seems to have a dual role like p53. They established mice with nestin-Cre-mediated neuronal deletion of c-Jun. The c-Jun-negative motor neurons blocked neuronal death after facial axotomy, although they still showed severe neuronal atrophy. Despite showing inhibition of neuronal death, the mice showed strongly diminished speed of target innervation regeneration after nerve injury. Furthermore, they showed reduced expression of several RAGs including cell adhesion molecules such as integrin α7β1 and CD44 (Jones et al., 1997;Werner et al., 2000) and neuropeptides such as galanin (Shadiack et al., 2001). This finding reinforces the notion that c-Jun has an important role in turning on the regeneration program after injury. This does not exclude the possibility that a lack of c-Jun affects non-neuronal cells in these mice in addition to neurons, because the nestin-Cremediated deletion of c-Jun also occurs in glial cells. c-Jun can drive Schwann cell dedifferentiation and is upregulated in nonneuronal cells like Schwann cells after axotomy. In line with this fact, mice with conditional inactivation of c-Jun in Schwann cells showed strikingly delayed myelin loss after sciatic nerve injury (Parkinson et al., 2008). Because rapid removal of myelin enables axon regeneration in the PNS (Vargas and Barres, 2007), these mice would be expected to have compromised functional recovery after nerve injury. Further study is required to elucidate these points.

ATF3
Activating transcription factor 3 (ATF3) is a stress-inducible transcription factor, which binds to the ATF/CRE site (Hai and Hartman, 2001). ATF3 is not normally found in neuronal cells but is highly expressed in response to nerve injury. Thus, ATF3 is known as an injury marker in the nervous system (Tsujino et al., 2000;Nakagomi et al., 2003;Ohba et al., 2003). The role of ATF3 diverges according to the cellular context and/or the components of the ATF3-containing complex. ATF3 forms homo-and heterodimers to function as both a repressor and activator of genes (Hai and Hartman, 2001). ATF3 seems to be a major determinant of the intrinsic growth state of neurons. ATF3 expression is correlated with the survival and activation of intrinsic growth ability in CNS neurons (Ohba et al., 2003;Campbell et al., 2005;Zhang et al., 2011b). Overexpression of ATF3 in cultured superior cervical ganglion (SCG) neurons protects against cell death after NGF withdrawal, a popular apoptotic stimulus, while it enhances neurite outgrowth in both SCG neurons and DRG neurons Seijffers et al., 2006). Mice overexpressing ATF3 under the control of the neuron-specific Thy1.2 promoter revealed enhanced neurite elongation in vitro and in vivo after sciatic nerve injury (Seijffers et al., 2006). ATF3 is also up-regulated as an adaptive response in cultured demyelinated DRG neurons and increases the speed of axonal mitochondrial transport, probably to maintain axonal homeostasis . To achieve these roles, ATF3 has been believed to dimerize with c-Jun in a contextspecific manner. The concurrent expression of c-Jun with ATF3 is frequently observed in damaged neurons after nerve injuries, including peripheral axotomy and Middle Cerebral Artery (MCA) occlusion Ohba et al., 2003). There is also in vitro evidence to show an interaction between ATF3 and c-Jun (Chen et al., 1996;Nakagomi et al., 2003). However, an interaction between ATF3 and c-Jun has not been reported in vivo to date. It is likely that some previously unidentified signaling pathway or associated proteins, which are activated by nerve injury, are involved in facilitating the dimerization of ATF3 and c-Jun.
Information about the target genes of ATF3 is still limited, although ATF3 can bind to an atypical ATF/CRE site in the Heat shock protein (Hsp) 27 promoter (Benn et al., 2002;Nakagomi et al., 2003). The aforementioned ATF3 transgenic mouse altered the expression of a set of RAGs in non-injured DRG neurons (Seijffers et al., 2006). They include c-Jun, Hsp27 which prevents neuronal death (Lewis et al., 1999), Small prolinerich repeat protein (SPRR) 1A which promotes axonal outgrowth (Bonilla et al., 2002), and CAP23 which is functionally related to GAP43 (Bomze et al., 2001). These genes might be candidate targets of ATF3. If a potential counterpart like c-Jun is activated at the same time, much greater induction of much more RAGs may be observed in non-injured DRG neurons.
To clarify the importance of ATF3 in injured neurons, ATF3 knockout mouse will be a useful tool (Hartman et al., 2004). To date, ATF3-deficient mouse lines have revealed no obvious abnormalities in neurite elongation or survival after nerve injury. This is probably because the mouse is not a complete null mutant, but still expresses the DNA-binding domain and leucine zipper domain of the protein, resulting in abundant expression of a small fragment protein and mRNA after nerve injury (Seijffers et al., 2007) and our observations.

STAT3
Signal transducer and activator of transcription 3 (STAT3) is activated in response to growth factors, cytokines, and hormones that are known to play protective role after nerve injury (Dziennis and Alkayed, 2008). Binding of these growth factors or cytokines to their cognate receptors, including the common receptor subunit gp130, activates Janus kinase (JAK), which phosphorylates tyrosine residues on the cytoplasmic portion of the receptor complex, and then phosphorylates STAT3. In general, axotomy increases the expression and phosphorylation of STAT3 (Schwaiger et al., 2000;Qiu et al., 2005). To examine the role of STAT3 in vivo, Schweizer et al. used mice with neuron-specific ablation of STAT3 created by Cre recombinase expression under the control of the neurofilament light chain (NF-L) promoter (Schweizer et al., 2002). The study indicated that STAT3 contributes to the survival of motor neurons after facial nerve lesions through activation of Reg2 and Bcl-XL (Gonzalez-Garcia et al., 1995;Livesey et al., 1997), which promote neuronal survival. Schweizer et al. did not investigate axonal regeneration using these mice. However, other studies implicated an effect of STAT3 on nerve regeneration using the peripheral conditioning injury paradigm and optic nerve injury model. The conditioning lesion paradigm is a widely accepted model in which elevated intrinsic growth ability caused by prior peripheral nerve axotomy facilitates subsequently lesioned dorsal column axons to regenerate over a short distance (Richardson and Issa, 1984;Neumann and Woolf, 1999). Qiu et al. demonstrated that STAT3 activation is necessary for increased growth ability of DRG neurons and improved axonal regeneration in the spinal cord after conditioning injury (Qiu et al., 2005). Furthermore, the deletion of suppressor of cytokine signaling 3 (SOCS3), which is a negative regulator of the JAK/STAT3 pathway, promotes axonal regeneration after optic nerve injury (Smith et al., 2009). Thus, a considerable body of evidence points to an involvement of STAT3 in nerve regeneration.
Apart from the well-understood job of STAT proteins in transmitting transcriptional signals from the cell surface to the nucleus, a new finding reveals that mitochondrial localization of STAT3 contributes to oxidative phosphorylation, leading to regulation of respiration (Gough et al., 2009). Furthermore, other studies claim that unphosphorylated STAT3 can be also imported into the nucleus independent of phosphorylation and, once there, regulate gene transcription by binding to other nuclear proteins (Liu et al., 2005;Yang et al., 2005). Although the physiological relevance of these newly discovered functions of STAT3 remains to be established, these findings expand our classical understanding of gene regulation by STAT3.

COOPERATIVE TRANSCRIPTIONAL REGULATION BY INJURY-INDUCIBLE TRANSCRIPTION FACTORS
As mentioned above, numerous candidate injury-inducible transcription factors have been identified to date. How do those injury-inducible transcription factors communicate with each other and/or participate in the complex molecular machine? How do they access distinct RAGs with common or different injuryresponsible DNA elements? We have assumed that one of the best ways to answer the questions is via the promoter analysis of a representative nerve RAG, which gives us clues to resolve these mysteries. From this point of view, damage-induced neuronal endopeptidase (DINE) is considered to be an ideal representative gene to provide further information about how injury-inducible Frontiers in Molecular Neuroscience www.frontiersin.org transcription factors cooperate with each other during nerve regeneration.

NERVE REGENERATION-ASSOCIATED GENE DINE
Previous large-scale screening studies from our laboratory and others have identified numerous potential RAGs (Kiryu et al., 1995b;Tanabe et al., 1999;Bonilla et al., 2002;Costigan et al., 2002;Tanabe et al., 2003). The aim of our studies has been to understand the molecular basis underlying nerve regeneration. In addition, another important goal has been to find pan-nerve injury-inducible genes. These aims are based on the idea that there must be common injury-responsive transcriptional machinery in both the PNS and CNS, which triggers RAG expression and will be a potential key to enabling robust CNS regeneration to proceed in the future. In a search for RAGs, we identified DINE (Kiryu-Seo et al., 2000), which another group isolated independently as XCE later registered as endothelin converting enzyme-like 1 (ECEL-1); . The most intriguing property of DINE is its extreme transcriptional response against various kinds of nerve injuries, both in the PNS and CNS, including motor, sensory and sympathetic nerve injuries, brain and spinal cord trauma, and cerebral ischemia (Kiryu-Seo et al., 2000;Boeshore et al., 2004), which led to its name (Kiryu-Seo et al., 2000). The enhanced expression of DINE is strikingly restricted to neuronal cells, and not seen in glial cells. DINE mRNA induction is always accompanied by the induction of ATF3, in various injury models Ohba et al., 2004). DINE mRNA is significantly induced by LIF treatment and NGF withdrawal both in vitro and in vivo, suggesting that c-Jun, ATF-3, and STAT3 are candidate transcription factors downstream of LIF-gp130 signaling and JNK signaling for transactivation of DINE gene Kiryu-Seo and Kiyama, 2004). At the protein level, DINE functions as a neuron-specific membrane-bound metalloprotease. It shares homology with neprilysin (NEP) and endothelin converting enzyme (ECE), which degrade or process neuropeptides such as amyloid β and endothelin, which play important roles in Alzheimer pathology and migration from the neural crest, respectively (Xu et al., 1994;Iwata et al., 2000). DINE is supposed to have a critical role in injured neurons, although its substrate remains unknown (Shirotani et al., 2001). DINE-deficient mice die immediately after birth due to respiratory failure Nagata et al., 2010). This is caused by the failure of distal axonal arborization into muscle, resulting in poor formation of neuromuscular junctions (NMJ) (Nagata et al., 2010). Because DINE deficiency leads to abnormal axon behavior during development, DINE is expected to function during nerve regeneration as well (Nagata et al., 2006(Nagata et al., , 2010. Thus, DINE is considered to be a fascinating target gene with which to examine the transcriptional mechanisms involved in the response to nerve injury.

SP1-MEDIATED TRANSCRIPTIONAL COMPLEX
Using the DINE promoter, we identified activation of the Sp1mediated transcriptional machinery in response to nerve injury . Here, we describe this machinery and propose a potential explanation for how a cohort of nerve injury-induced transcription factors participates in the dynamic alteration of gene expression during nerve regeneration.
The 5 -flanking region of the DINE gene is thought to contain the injury-responsive element. DINE promoter activity was enhanced in response to LIF treatment and NGF withdrawal in cultured DRGs, mimicking the endogenous response of DINE mRNA . Co-expression of ATF3, c-Jun, and STAT3 increased DINE promoter activity by a small amount. This is because the efficient dimerization of c-Jun and ATF3 does not occur in this context. It is unclear what makes c-Jun and ATF3 dimerize, although the heterodimer is believed to promote axonal regeneration . There may exist some unidentified signal that makes the heterodimer stable in injured neurons. Thus, we used a forced heterodimer of ATF3 and c-Jun in our experiment. The forced heterodimer upregulated DINE promoter activity tremendously, with a further increase observed in the presence of STAT3, suggesting that these injury-inducible transcription factors contribute to the regulation of the DINE promoter. Unexpectedly, c-Jun, ATF3, and STAT3 did not directly bind to a specific element within the DINE promoter. Instead, Sp1 directly bound to the GC-rich region located in the region proximal to the transcription start site of the DINE promoter, and could function as a scaffolding protein to recruit c-Jun, ATF3, and STAT3 to elicit their functional synergy (Figure 2; Kiryu-Seo et al., 2008). Of the complex, ATF3, which makes subsequent dimerization with c-Jun, may be the most critical, because ATF3 is specifically expressed after nerve injury. The findings of an in vivo study using chromatin immunoprecipitation (ChIP) assay supported the existence of such a complex in injured hypoglossal neurons. We could not rule out the possibility that these transcription factors, like c-Jun, ATF3, and STAT3, regulate the DINE gene by binding to individual binding sites separately in an unidentified enhancer region, and that a combination of these proposed transcriptional mechanisms regulates the expression of DINE. However, consistent with our finding, it has been reported that gene promoters without the AP1 site or without the STAT3 binding site were activated by c-Jun or STAT3 through an interaction with Sp1 (Kardassis et al., 1999;Chen and Chang, 2000). Importantly, the mechanism by which Sp1 may provide c-Jun/ATF3/STAT3 with a platform would be practical and effective when increased gene expression is required in the event of a fatal emergency such as neuronal injury. Many neuronal genes contain the Sp1 binding site (Ross et al., 2002), suggesting that nerve injury-inducible transcription factors are capable of accessing many genes without specific binding sites.

MULTIFUNCTIONAL TRANSCRIPTION FACTOR SP1
Sp1 is a general transcription factor that binds to GC-rich motifs with high affinity. Sp1 was thought to serve mainly as a constitutive activator of housekeeping genes in a classical view. Growing evidence suggests that Sp1 is a multifunctional transcription factor. At the transcriptional level, Sp1 is not induced in injured neurons, but is expressed constitutively. The regulation of Sp1 is important at the protein level. The activity of Sp1 is regulated by post-translational modifications, as well as by interaction partners. Phosphorylation, acetylation, sumoylation, ubiquitination, and glycosylation are among the post-translational modification that can influence the transcriptional activity and stability of Sp1 (Hung et al., 2006;Tan and Khachigian, 2009). The accessibility of injury-inducible transcription factors may be determined by the modification of Sp1, suggesting the possibility that different modifications of Sp1 recruit different injury-inducible transcription factors. The activity of Sp1 is also regulated by interactions or interplay with other transcription factors such as p53, c-myc, Smad, AP-2, and E2F-1 (Wierstra, 2008). These additional transcription factors may participate in the Sp1/c-Jun/ATF3/ STAT3 complex to yield maximum effects in injured neurons (Figure 2).
The involvement of Sp1 is illuminated in the neurodegenerative disease Huntington disease (HD). Sp1 is induced and acetylated under the conditions of oxidative stress observed in HD neurons (Ryu et al., 2003b;Qiu et al., 2006). Sp1 induction confers a resistance to oxidative stress-induced cell death through transcriptional activation (Ryu et al., 2003a). In HD pathology, mutant huntingtin (Htt) interacts with Sp1 and core components of the transcriptional machinery, leading to disruption of transcriptional activation of neuronal genes such as the dopamine D2 receptor (Dunah et al., 2002;Freiman and Tjian, 2002;Zhai et al., 2005). Conversely, others have reported that a decrease in the Sp1 level has a beneficial effect on HD pathology (Qiu et al., 2006;Ravache et al., 2010). These results seem to be contradictory. The reason for this may be attributable to the diverging role of Sp1. Recently, Sleiman et al. demonstrated that a GCrich DNA-binding drug mithramycin (MTM), which competes with Sp1 for its binding site, prevents neuronal death in the HD model of Drosophila as well as in cultured cortical neurons under conditions of oxidative stress. The protective role depends on the selectivity of MTM, meaning that MTM selectively binds to the GC-box of proapoptotic genes like myc but not that of antiapoptotic genes like p21 (Sleiman et al., 2011). The mechanism of action remains unknown; however, this work suggests that Sp1 could be a target of therapeutic strategies.
Interestingly, Sp1 interacts with chromatin-modifying factors such as p300, histone acetylases (HATs) and histone deacetylases (HDACs) (Ryu et al., 2003a;Hung et al., 2006), while the Sp1 target sequence, the GC-box, can be a substrate for DNA methylation. It is therefore, an attractive possibility that Sp1 is a target for epigenetic regulation. Taking this possibility into consideration, our finding of the Sp1-mediated mechanism, which provides a platform the injury-inducible transcription factors, stretches the possibility of understanding global gene regulation during nerve regeneration.

POTENTIAL INVOLVEMENT OF EPIGENETIC MODIFICATION
Epigenetic modification of both histones and DNA is emerging as a fundamental mechanism by which neurons adapt their transcriptional responses to environmental cues (Jaenisch and Bird, 2003;Riccio, 2010). Histone modification including acetylation, methylation, phosphorylation, ubiquitination, and sumoylation modifies local chromatin structure or provides binding sites for non-DNA-binding chromatin proteins. The combination of such modifications can induce the formation of transiently closed or open chromatin domains. Open chromatin is associated with active gene expression, while closed chromatin is associated with DNA compaction and gene repression.
Chromatin-modifying enzymes are found in large multiprotein complexes that are recruited to gene promoters by the transcription factors that bind to specific DNA sequences and confer target specificity (Borrelli et al., 2008). In neurons, HATs and HDACs are the best-characterized chromatin-modifying enzymes. Multiple lines of evidence suggesting that epigenetic regulation is involved in neurodegenerative pathology (Julii et al., 2010;Ma et al., 2010), and that the use of HDAC inhibitors combats neurodegenerative conditions in cellular and disease models (Chuang et al., 2009), have been accumulated. In this context, it is conceivable that epigenetic regulation by chromatin modifiers influences nerve regeneration (Figure 3). Indeed, nerve injuries such as optic nerve injury and hypoglossal nerve injury enhance the expression of HDAC family proteins (Pelzel et al., 2010) and our unpublished data. Neuron-restrictive silencer factor (NRSF) is up-regulated after sciatic nerve injury and recruits HDACs to suppress the expression of Nav1.8 sodium channels, u-opioid receptor (MOP) and the voltage-gated potassium channel Kv4.3 in injured DRG neurons (Uchida et al., 2010a,b). Usually, NRSF represses the expression of neuronal genes in non-neuronal cells, although it can be an activator in some situations (Ballas et al., 2005). This suggests that the normal pattern of gene expression in neurons can be reversed in injured neurons to allow expression of non-neuronal genes as observed in the genes encoding Frontiers in Molecular Neuroscience www.frontiersin.org FIGURE 3 | The possible involvement of epigenetic modification during nerve regeneration. In addition to the involvement of numerous transcriptional factors shown in Figure 1, the epigenetic modification of chromatin structure might affect the gene expression following nerve injury. Both histone modification including acetylation and phosphorylation and DNA modification such as methylation are associated with active gene expression as well as gene repression. Furthermore, microRNAs are implicated as potent silencers of gene expression.
ERK and Shc (Kiryu et al., 1995a;Tanabe et al., 1998 (Jessen and Mirsky, 2005;Chen et al., 2011;Jacob et al., 2011). It is implied that a similar mechanism works in Schwann cells as well as in neurons after nerve injury. DNA methylation at CpG dinucleotides in the genome is also a major epigenetic mechanism, and is associated with a condensed structure and transcriptional repression. DNA methylation status seems to be flexible rather than fixed to allow adaptation to environmental changes. Damaged cells are no longer able to maintain their prior level of DNA methylation (Ramchandani et al., 1999;Endres et al., 2000). Using the conditioning lesion model, which enables lesioned dorsal column axons to undergo lengthy regeneration in a segment of peripheral nerve transplanted into spinal cord (Hoffman, 2010), Iskandar et al. reported that the injury suppresses DNA methyltransferase (Dnmt) protein levels in spinal cord and that DNA methylation is decreased globally (Iskandar et al., 2010). The administration of folic acid, which is required for DNA methylation through the folate pathway, restored Dnmt protein expression as well as axonal regeneration. Although it is not apparent which cells are responsible for this effect, these findings imply that DNA methylation is a critical factor for gene regulation after nerve injury.
Recently, a new player has emerged as a key molecule in the epigenetic gene regulation of many biological processes. microRNAs (miRNAs) act as potent silencers of gene expression via translational repression and/or mRNA destabilization. Interestingly, some miRNAs are especially abundant in the nervous system, suggesting that they might be particularly important there. Exciting work has been reported in the fields of neurogenesis, plasticity, and neurodegeneration (Eacker et al., 2009;Siegel et al., 2011). Although the involvement of miRNAs in nerve regeneration is not clear, the altered expression of some miRNAs has been validated in DRG neurons after sciatic nerve injury (Zhang et al., 2011a;Zhou et al., 2011). A recent elegant study demonstrated that miRNA 206 in muscle promotes regeneration of neuromuscular synapses (Williams et al., 2009). Gaining further understanding of the roles of epigenetic modification and miRNAs in nerve regeneration is the next critical step and will advance our understanding of the gene regulation mechanisms operative in injured neurons.

CONCLUSIONS
The challenging goal is to reach a sufficient understanding of the global transcriptional machinery in injured neurons to realize robust CNS regeneration. Accumulating evidence supports the possibility that injury-inducible transcription factors like c-Jun, ATF3, and STAT3 are involved in the intrinsic regenerative growth ability in vivo. To achieve orchestrated expression of distinct RAGs, the transcriptional network and the multiprotein complex could function in damaged neurons in response to environmental cues. The Sp1 transcriptional machinery described in this review provides one explanation for how numerous RAGs are regulated simultaneously in injured neurons. Additionally, epigenetic regulation is also emerging as a crucial factor in controlling gene regulation during nerve regeneration. We are still far from a comprehensive understanding of how the interplay of transcriptional and epigenetic processes contributes to nerve regeneration. It will be challenging to define the exact mechanism underlying global transcriptional regulation in the nuclei of injured neurons.
As a biological tool that is currently available for studying nerve regeneration in vivo, knockout mice have proven to be very valuable (Zheng et al., 2006). However, for technical reasons, only a few knockout mice for the transcription factors described here have been studied using this method to date. Most transcription factors are critical in development and normal cellular function, so their knockout in mice would be lethal during development and/or the development of mice would be influenced by their ablation. The best approach is to perform gene deletions specifically in injured neurons using Cre/loxP technology. For this purpose, transgenic mice with Cre recombinase expressed only in injured neurons appear to be a suitable approach. Ongoing work using this approach will improve our understanding of how nuclear events activate the intrinsic ability to drive axonal growth and will provide clues to specific therapeutic strategies.

ACKNOWLEDGMENTS
This work was supported by Grants from The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) and a Grant-in-Aid for Young Scientists (B). We would like to thank members of the laboratory for helpful discussions.

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