Complement Components Showed a Time-Dependent Local Expression Pattern in Constant and Acute White Light-Induced Photoreceptor Damage

Background: Photoreceptor cell death due to extensive light exposure and induced oxidative-stress are associated with retinal degeneration. A correlated dysregulation of the complement system amplifies the damaging effects, but the local and time-dependent progression of this mechanism is not thoroughly understood. Methods: Light-induced photoreceptor damage (LD) was induced in Balb/c mice with white light illumination either for 24 h with 1000 lux (constant model) or 0.5 h with 5000 lux (acute model). Complement protein and mRNA expression levels were compared at 1 and 3 days post-LD for C1s, complement factor B (CFB), mannose binding lectin A, mannose-binding protein-associated serine protease 1 (MASP-1), C3, C4, C9, and complement factor P in retina and RPE/choroid. Histological analyses visualized apoptosis, microglia/macrophage migration, gliosis and deposition of the complement activation marker C3d. Systemic anaphylatoxin serum concentrations were determined using an ELISA. Results: Apoptosis, gliosis and microglia/macrophage migration into the outer nuclear layer showed similar patterns in both models. Local complement factor expression revealed an early upregulation of complement factor mRNA in the acute and constant light regimen at 1 day post-treatment for c1s, cfb, masp-1, c3, c4 and c9 in the RPE/choroid. However, intraretinal complement mRNA expression for c1s, cfb, c3 and c4 was increased at 1 day in the constant and at 3 days in the acute model. A corresponding regulation on protein level in the retina following both LD models was observed for C3, which was upregulated at 1 day and correlated with increased C3d staining in the ganglion cell layer and at the RPE. In the RPE/choroid C1s-complex protein detection was increased at 3 days after LD irrespectively of the light intensities used. Conclusion: LD in mouse eyes is correlated with local complement activity. The time-dependent local progression of complement regulation on mRNA and protein levels were equivalent in the acute and constant LD model, except for the intraretinal, time-dependent mRNA expression. Knowing the relative time courses of local complement expression and cellular activity can help to elucidate novel therapeutic options in retinal degeneration indicating at which time point of disease complement has to be rebalanced.


INTRODUCTION
Exposure to sunlight has been suggested years ago as a risk factor for the development and progression of retinal degeneration and particularly age-related macular degeneration (AMD) [reviewed in Sui et al. (2013)]. Extensive light exposure induces apoptosis-driven photoreceptor loss in humans and mice (Wenzel et al., 2005;Organisciak and Vaughan, 2010). This is dependent on light intensity, light quality and exposure time (Wenzel et al., 2005). Several mechanisms are correlated with light-induced photoreceptor degeneration (LD), including an altered retinoid metabolism, increased oxidative stress and immunoreactions (Maeda et al., 2009;Pujol-Lereis et al., 2016). The exact interplay of these cascaded events still needs to be deciphered. Time-dependent analysis of the transcriptome after LD in mice revealed a clustered gene expression which imply a strongly regulated temporal coordination of stress gene expression in the light-damaged retina (Choi et al., 2001). However, we still lack an accurate understanding of the functional relevance of this timedependent gene expression following LD and data that reveal how the transcriptomics are translated into protein expression.
The aim of the present study was to identify a LD-dependent transcriptional and protein expression profile of components of the complement system. Complement involvement in LD had been shown in a wide spectrum of white light treatment models, including mice/rats which were illuminated with light intensities between 1000 and 10000 lux for 1 h-10 days and evaluated after a recovery time up to 7 days (Rohrer et al., 2007;Rutar et al., 2011;Hadziahmetovic et al., 2012;Song et al., 2012). Comparison and generalization of these studies is difficult. Especially, since we know, that different light intensities, low and bright light, induce different apoptotic pathways in the retina (Roca et al., 2004) and prolonged recovery times influence the number of apoptotic cells following LD (Zhang et al., 2005;Jiao et al., 2015). Therefore, we chose a short-term, acute (0.5 h) and an intermediate, constant (24 h) exposure time with high (5000 lux) and medium (1000 lux) white light intensities to compare the effects of illumination on complement factor expression after 1 and 3 days of treatment (Supplementary Figures S1A-C).
The complement system is a humoral part of the innate immune system which bridges the adaptive and innate Abbreviations: CFB, complement factor B; CFP, complement factor P; ELISA, enzyme linked immunosorbent assay; LD, light-induced photoreceptor damage; MASP-1, mannose-binding protein-associated serine protease 1; MBL-A, mannose binding lectin A; ONL, outer nuclear layer; RPE, retinal pigmented epithelium; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling. immune response. Genetic polymorphisms in the complement cascade, complement depositions in drusen and drugs targeting factors of the complement system as potential AMD-therapeutics, supposed a connection between retinal degeneration, complement expression and light irradiation (Anderson et al., 2010;Weber et al., 2014;Volz and Pauly, 2015). The complement system is composed of over 40 interacting proteins which are successively involved in the reaction. The cascade is activated by three distinct pathways: the classical, lectin and alternative pathways. Activation of all three pathways results in the cleavage of complement component C3 to C3a and C3b, with the latter being required for the cleavage of C5 to C5a and C5b as well as the formation of the terminal complement complex. The complement system finally results in three main biological functions: tagging of cell waste and pathogens by opsonins (e.g., iC3b), attraction and activation of immune cells by anaphylatoxins (e.g., C3a, C5a) and cell lysis by the terminal complement complex (C5b-C9). The activity of the complement system is tightly controlled under physiological conditions, but it can be dysregulated in pathological events resulting in an autoreactive damage of cells.
To obtain a conclusive picture, here we correlate the timedependent glial activation pattern, neuronal cell loss and expression of different complement factors on mRNA and, importantly, on protein level in the retina and RPE/choroid. Our results confirm previously described expression patterns regarding c1s, c3 and c4 and we added analyses regarding cfb, masp-1, mbl-a, c9 and cfp. We describe an early gene upregulation in the RPE/choroid after 1 day of LD in both tested light models and in the retina for the constant light regime. Complement gene expression showed a delayed increase in the retina at 3 days following acute LD. We report for the first time, that C3 and C1s were altered on protein level in Western blots following both constant and acute LD either in the retina or in the RPE/choroid, respectively. Overall the studies revealed that C3, C1s and C9 could serve as time-dependent marker proteins for complement involvement for LD in mice.

Animals, Ethics Statement, and LD
Six to nine week old BALB/c mice obtained from Charles River Laboratories (Wilmington, MA, United States) and tested homozygous for the L450 variant of RPE65 (Wenzel et al., 2001) were kept in cyclic light (12 h on/12 h off, lights on at 7 am, light intensity approx. 400 lux) (Supplementary Figures S1A-C). Mouse experiments were strictly performed according to the guidelines of replacement, refinement, and reduction of animals in research (Russell and Burch, 1959) and approved by the committee on the ethics of animal experiments of the regional agency for animal health Regierung der Oberpfalz, Veterinärwesen (54-2532.1-04/11). To induce LD in mice, they were transferred to cyclic dim light (<100 lux) for 5 days and followed by darkadapted period of 18 h. As the severity of retinal phototoxicity in rodents depends on the circadian rhythm (Vaughan et al., 2002) the light damage experiments were always performed in the early morning according to previously described protocols (Braunger et al., 2013a;Boneva et al., 2016). Briefly, mice were isolated placed in reflective cages and exposed to diffuse cool, white fluorescent light coming from the top of the cage for the constant LD model with an intensity of 1000 lux for 24 h and for the acute LD model with 5000 lux for 0.5 h. The average light intensity was measured on the cage floor. After light exposure, mice were allowed to recover first for 6 h in dim light, and then brought back to normal cyclic light conditions until sacrifice. The effects of LD were evaluated 1 and 3 days after light exposure (Supplementary Figures S1A-C).

Antibodies
Used antibodies are listed in Supplementary Table S1. The polyclonal anti-mouse CFP serum was generated by sequential immunizations of rats with recombinant murine CFP as described previously .

Terminal dUTP Transferase Mediated Nicked-End Labeling (TUNEL)
Apoptotic cell death was analyzed by TUNEL labeling using the Apoptosis Detection System (DeadEnd Fluorometric TUNEL, Promega). The light exposed and control retinae were investigated following manufacturers' instructions and protocols published previously (Braunger et al., 2013b(Braunger et al., , 2015Kugler et al., 2015). For quantitative analysis, the number of TUNEL-positive nuclei in mid-horizontal sections throughout the entire retina was counted and normalized to the area of the ONL. Indicating ONL cell density DAPI-positive cells were counted within central retina (100 µm area).

Gene Expression Analyses
One eye from each animal was enucleated and hemisected for total RNA extraction. To this end, RPE/choroid and retina were removed from eyecups and immediately deposited in RNA stabilizer (RNAlater, Qiagen, Hamburg, Germany). RNA was isolated using Nucleospin RNA/Protein Kit (Macherey-Nagel, Düren, Germany) and reverse-transcribed to cDNA using QuantiTect Reverse Transcription Kit (Qiagen, Hamburg, Germany) according to the manufacturer's protocol. Mouse liver cDNA was prepared equally.
Quantitative real-time PCR (qRT-PCR) was performed on a Rotor-Gene Q PCR cycler (Qiagen, Hamburg, Germany) using mouse gene-specific primer pairs (Supplementary Table S2) and Rotor Gene Sybr green PCR Kit (95 • C for 5 min, 40 cycle with 95 • C for 5 s and 60 • C for 10 s; Qiagen, Hamburg, Germany). A relative gene expression was calculated using mRNA levels of actin as a housekeeper with the CT method ( CT = C T gene of interest -C T actin) ( Table 1)

Western Blot
Protein isolation of RPE/choroid and retina (one eye from each animal) was performed with Nucleospin RNA/Protein Kit (Macherey-Nagel, Düren, Germany). Samples were separated on a reducing 10% SDS-PAGE and transferred onto polyvinylidene difluoride membranes. Membranes were soaked in blocking solution [5% BSA/PBS-T (PBS, 0.1% Tween 20), 1 h] and subsequently incubated with primary antibodies diluted in blocking solution (Supplementary Table S1, overnight, 4 • C). After washing steps, membranes were treated with detection antibodies diluted in blocking solution (Supplementary Table S1, 1 h) and developed with Lumi-Light blotting substrate (Roche Diagnostics GmbH, Mannheim, Germany) or WesternSure PREMIUM Chemiluminescent Substrate (LI-COR, Bad Homburg, Germany).

Software and Statistical Analysis
Data were statistically analyzed using GraphPad Prism 5 (GraphPad Software, San Diego, CA, United States). Western blot and microscopy pictures were evaluated using ImageJ (Schneider et al., 2012).

Light-Treatment Caused Damage in the Retina
Photoreceptor cell death is a hallmark in LD (Grimm and Remé, 2013). In order to determine whether constant (1000 lux, 24 h) or acute (5000 lux, 0.5 h) white light treatment differ in the progression of LD, we counted TUNEL-positive cells indicating apoptosis (Figures 1A-F), and DAPI-positive cell nuclei (Figures 1A-E,G), displaying photoreceptor density in the ONL. Cell apoptosis occurred in the ONL of mice treated

Complement Factors Were Locally Expressed in the Untreated Albino Mouse Eye
The aim of the study was to evaluate the role of the local complement system in LD. First, we compared complement factor expression on mRNA and protein level in the retina and RPE/choroid of untreated control albino mice ( Table 1). Complement factors c1s, cfb, c3, c4 and cfp mRNA were constantly expressed in the retina and RPE/choroid of untreated Balb/c mice ( Table 1). We determined a significantly higher expression level for c1s and c3 mRNA in the RPE/choroid compared to the retina ( Table 1) and vice versa a higher expression of cfp mRNA in the retina than in the RPE/choroid ( Table 1). There was no significant difference in the tissue-specific expression of cfb and c4 in the eye ( Table 1). C9 mRNA and complement factors of the lectin pathway, mbl-a and masp-1, were detected only in a few animals of the control group (Table 1 and Figures 2, 3).
All tested complement factors were found on protein level in the RPE/choroid ( Table 1). In general, the retina was less positive for complement deposition than the RPE/choroid as we observed no CFB or C4 protein and low C3 protein levels in the untreated retina (Table 1 and Figures 4-6). The increased tissue-specific mRNA expression for c3 in the RPE/choroid ( Table 1) was confirmed by increased protein detection in Western blots (Table 1 and Figures 4C, 6D) and immunohistochemistry ( Figure 5). Higher molecular weight complexes containing either C1s or C9 showed also a 4-5-fold enhanced protein detection signal in the RPE/choroid than in the retina (Table 1,  White Light Increased c1s, cfb, c3, and c4 Expression in the Retina Dependent on the Recovery Time, But Decreased cfp and c9 Expression Complement activation was previously described as a cause for LD (Rohrer et al., 2007;Rutar et al., 2011). We asked the question, whether there is a difference in complement factor expression following constant (1000 lux, 24 h) or acute (5000 lux, 0.5 h) LD in the eye (Figures 2, 3). We identified a significant increase of c1s, cfb, c3 and c4 mRNA expression in the retina following both light treatment regimens (Figures 2A,B,E,F). Interestingly, there was a difference in the reaction regarding the recovery time after light treatment. Mice treated with 1000 lux for 24 h (constant) showed an enhanced complement gene expression in the retina after 1 day recovery (Figure 2, white bars), which correlated with the time-dependent apoptosis in the ONL ( Figure 1F). In contrast, mice treated with 5000 lux for 0.5 h (acute) showed a change in c1s, cfb, c3 and c4 expression in the retina mainly 3 days after light illumination (Figure 2, black bars).
The terminal complement factor c9 and the alternative cascade stabilizer cfp were constantly expressed in the healthy retina ( Table 1), but the expression signals were decreased in the light treated animals (Figures 2G,H). Cfp mRNA expression was promptly regulated and a significant change was detected as early as 1 day post-treatment in both models ( Figure 2H). Positive animals were indicated as small numbers in the bars. Mean of untreated control shown as dotted line. * 0.01 < P < 0.05, * * 0.001 < P < 0.01, * * * 0.0001 < P < 0.001, and * * * * P < 0.0001 (ordinary one-way ANOVA, Dunnett's multiple comparisons test, with a single pooled variance).   Figure S4). CFB and C4 were not detected in retinae. * 0.01 < P < 0.05 (ordinary one-way ANOVA, Dunnett's multiple comparisons test, with a single pooled variance).     , Figures 2, 3). We observed a significant increase of mRNA for cfb and c4 (Figures 3B,F) as well as an elevated signal for c1s, c3 and c9 at 1 day compared to 3 days post-LD (Figures 3A,E,G). The mice treated with acute light (5000 lux, 0.5 h) showed a comparable expression as the constant light treated animals in the RPE/choroid (Figure 3). This was in contrast to the retinal expression pattern in the acute group, where we observed an increased mRNA expression only 3 days after LD but not immediately 1 day post-LD (black bars, Figures 2, 3). The complement factor expression in the RPE/choroid decreased at 3 days post-LD in both models (Figure 3). We emphasize that the gene expression levels for cfb and c3 even dropped below the normal expression levels of untreated mice (Figures 3B,E).

Similar Regulation of Complement Factors at mRNA Levels in Acute and Constant LD
The described complement gene expression studies (Figures 2, 3) suggested a pattern for the regulation of complement gene expression following LD. Indeed, when we plotted the gene expression profiles of all tested genes in one graph corresponding to the used light intensities and tested tissues (Supplementary Figure

C3 Deposition Was Increased after LD
Further, we investigated if the mRNA expression of complement genes corresponded to protein detection in the eye (Figures 4-6 and Supplementary Figures S4, S6, S7). The only significant changes in the retina after light treatment were observed in regard to C3-the central complement protein (Figures 4C, 5 and Supplementary Figure S6D). C3 protein concentrations were elevated in all light-treated mice retinae compared to the control in Western blots, irrespectively of the recovery time ( Figure 4C and Supplementary Figure S6D). C3d deposition, which is a cleavage product of C3b, was also increased in immunostainings of tissue sections following acute and constant LD (Figure 5). C3d-staining in the LD-retinae co-localized with GFAP-reactive Müller cells and astrocytes (Figure 5). The increased C3 protein detection in the retina correlated with increased c3 mRNA expression in the retina after LD (Figure 2E), except that the c3-transcription level in the acute model did not change after 1 day recovery ( Figure 2E). The trend for diminished c3 mRNA expression in the RPE/choroid complex 3 days after constant and acute LD ( Figure 3E) corresponded to decreased C3 protein detection in Western blots in the RPE/choroid (Figure 6D and Supplementary Figure S7A). Especially, the C3-alpha chain fragment (45 kDa) was missing after 3 days in the RPE/choroidcomplex ( Figure 6D and Supplementary Figure S7A). In contrast, we observed an increased C3d immunostaining in the RPE/choroid after LD (Figures 5B-E). C3d was deposited at the surface of the RPE-cells following both light regimens (Figures 5B-E). The differences in C3d-detection in Western Blots and immunostainings could be explained by the different specificities and affinities of the antibodies used here: the anti-C3 antibody in the Western Blots detected C3 irrespective of its activation pattern (Figure 6D and Supplementary Figure S7A) and in the immunostaining the antibody showed a selective specificity against the C3d activation product (Figure 5).

Classical Complement Pathway Initiator C1s-Protein Complex Accumulated in RPE/Choroid after LD
C1s is a protease, which acts in a high molecular weight protein complex with C1r and C1q. Western blots from the RPE/choroid of LD mice showed a significant increase of the C1scomplex (140 kDa) 3 days post-light treatment (Figure 6A and Supplementary Figure S7D). The 2-times higher C1s-complex signal in the RPE/choroid did not completely overlap with an elevated local c1s mRNA expression following light-treatment but could be a result of a delayed increase of C1s protein expression compared to its transcript in the retina and RPE/choroid at 1 day after constant and acute light illumination (Figures 2A, 3A). We did not observe a change in C1s protein deposition in the retina ( Figure 4A and Supplementary Figure S6A).

LD Resulted in Reduced Soluble C9-Complex Concentrations in the RPE/Choroid and Increased C5b-C9 Deposition in the Retina
C9 is the central protein of the terminal pathway of the complement system and forms the membrane attack complex together with C5b-C8. Soluble C9 protein detection in untreated mice was 4-fold higher in the RPE/choroid than in retina (Table 1, Figures 4D, 6F, and Supplementary Figures S6B, S7E). LD resulted in decreased soluble C9 levels at 3 days in the RPE/choroid in both treatment regimens compared to the control ( Figure 6F and Supplementary Figure S7E). This correlated with decreased c9 mRNA expression in the RPE/choroid following constant and acute white light-treatment after 3 days recovery ( Figure 3G). While the concentration of the terminal complement membrane attack complex, consisting of C5b-C9, was enhanced after light-treatment in the retinae (Supplementary Figure S5).

Müller Cells, Astrocytes, and Microglia/Macrophages Were Activated 1 Day after Light Treatment
Next we asked the question whether the local, time dependent expression of complement factors after LD is associated with local cellular activity. Hence, the activity of Müller cells and astrocytes ( Figure 5) as well as the migration of microglia/macrophages was assessed by immunostaining (Figure 7). GFAP-staining (a marker for gliosis) showed reactive Müller cells and astrocytes in all mice at 1 and 3 days after light treatment (Figure 5), but not in the untreated animals. It is of note that a co-localization of C3d and GFAP was observed in the light treated mouse retinae (Figure 5).
Iba-1 positive cells (a marker for microglia/macrophages) were sparsely localized in the plexiform layers and in the choroid of untreated mice (Figure 7A). A moderate recruitment of amoeboid microglia/macrophages into the ONL was observed after light treatment in both light regimens (white arrows, Figures 7B-D). An extended recovery time of 3 days after LD promoted microglia/macrophage migration into the photoreceptor layer (PRS) (black arrows, Figures 7C,E). We did not observe an obvious difference in the microglia/macrophage reaction pattern using constant or acute white light treatment.

LD Resulted in Increased Systemic C3a and C5a Concentrations
The interaction of local and systemic complement system in retinal degeneration is still under debate. We evaluated systemic complement involvement in mice after LD by determining serum concentrations of C3a and C5a, which are activation markers for all three complement pathways (Figure 8). The systemic reaction toward light treatment was very heterogeneous. We observed a partial increase of anaphylatoxin concentrations, but not in all animals, following LD (Figure 8). We identified a significant increase of C3a concentration after 1 day of constant light treatment (1000 lux, 24 h) ( Figure 8A).

Complement Factors Are Locally Expressed in the Healthy, Albino Mouse Retina
Dysregulation of the complement system in the eye is associated with retinal degeneration and damage of the blood-retinal barrier (Weber et al., 2014). However, we are still confronted with a chicken and egg problem in regard to retinal degeneration. At what time is which player activating the complement cascade in the eye? Is the systemic complement system involved, which is produced in the liver or by immune cells and then transported by the blood into the choroid? Or is the damage of the retinal blood barrier associated with locally produced complement factors by RPE cells and retinal cells? Successful treatment strategies for retinal degeneration depend on an effective on-site drug application and therefore our knowledge about regional and temporal complement involvement needs to be extended (Volz and Pauly, 2015).
In this study, we focused on eye tissue specific complement factor analysis, dissecting the retina from the RPE/choroid. In accordance with the literature we showed, that c3 and c1s mRNA were mainly expressed in the RPE/choroid of healthy albino mouse eyes (Luo et al., 2011;Gemenetzi and Lotery, 2015). Conversely, cfp mRNA was expressed at higher amounts in the retina and the local expression levels of cfb, c4 and c9 mRNA were equivalent in both eye tissues (Luo et al., 2013;Woodell et al., 2013). In contrast, genes involved in the lectin pathway, mbl-a and masp-1, were only detected in a few control mice in retinal and RPE/choroid samples. Lou et al. described also a lack of mbl and low masp expression in the mouse eye (Luo et al., 2011).
The tested complement factors in this study were soluble, secreted proteins which were deposited and acted independently of their mRNA synthesis site. A tissue-specific correlation of mRNA detection and protein deposition revealed only for C3 and MASP-1 an overlap of similar, local mRNA and protein levels. However, we detected enhanced mRNA levels for c1s in the RPE/choroid but more C1s protein in the retina than in the RPE/choroid. Additionally, we determined an equivalent expression of cfb, c4, and c9 mRNA in both tissues, but an enhanced tissue-specific protein detection either in the retina for CFB and C4 or in the RPE/choroid for C9.
In summary, we conclude that complement factors are produced by healthy eye tissues on mRNA and protein level. Therefore, local complement expression could play an important role in the modulation of the complement homeostasis in the mouse eye and should be evaluated in parallel on mRNA levels as well as on protein levels. Care has to be taken when concluding from immunolocalization of respective complement proteins to the cell types actually producing the proteins given their features as secreted proteins.

Complement Expression Is
Time-Dependently Altered in the Murine Eye as a Consequence of LD Rohrer et al. (2007), Rutar et al. (2011 suggested an involvement of the complement system in the degenerative processes in LD and described an upregulation of c3 mRNA expression 3 h after LD and with a plateau at d4-10 post-LD. We confirmed these results, describing a statistically significant difference in c3 mRNA expression at 1 day after constant and 3 days after acute light treatment compared to untreated animals ( Figure 2E). These previous studies did not report a decrease of c3 mRNA expression at 3 days post-LD (Rohrer et al., 2007;Rutar et al., 2011), which was characteristic for the studied mRNA expression in the constant and acute LD models in the RPE/choroid and the constant model in the retina (Figures 2E, 3E and Supplementary Figure S3). Likely reasons for the differences could be that in the study by Rohrer et al. (2007) the mice were treated with continuous white light without a low light recovery phase and in our study light treatment was used as a trigger mechanism for a maximum of 24 h. Rutar et al. (2011) used rats for LD. Perhaps LD-dependent complement expression is different in murine and rat retina. This hypothesis is supported by novel data of the same group showing, that for pigmented light treated mice c3 mRNA expression decreases after a peak expression at d5 again in contrast to c3 expression in rats (Rutar et al., 2011;Natoli et al., 2016) Additionally to the central complement component C3, we report a tissue-and LD model-dependent increase of C1s mRNA and protein after LD (Figures 2A, 3A, 4A, 6A and Supplementary Figures S6A, S7D). The upregulation of this classical pathway activation factor was also described previously for LD in rats (Rutar et al., 2011). We emphasized the function of C1s in LD by showing for the first time an elevated C1sprotein complex accumulation in the RPE/choroid complex after LD (Figure 6A and Supplementary Figure S7D). C1s is a protease which acts in concert with C1q and C1r and cleaves components of the classical complement pathway. This could implicate an involvement of the classical complement pathway at the retinal-blood-barrier after LD. It was already proposed that the C1 complex could be involved in retinal degeneration, since a single nucleotide polymorphism in the C1-inhibitor is associated with AMD (Ennis et al., 2008). However, a deficiency in C1q did not result in a significant difference of retinal structure following LD compared to wild-type mice (Rohrer et al., 2007).
The possible involvement of the classical (C1s) pathway in LD requires the activity of molecules downstream of the activation step to execute the full biological function of the complement system in LD. We showed in accordance with previous reports, that c4 mRNA, a part of the classical/lectin C3/C5-convertase, is upregulated in the retina and RPE/choroid following LD (Figures 2F, 3F; Rohrer et al., 2007;Rutar et al., 2011), but we could not correlate changes on protein level for C4 ( Figure 6E and Supplementary Figure S7H), probably due to antibody detection problems. Nevertheless, this complement component should be further studied in retinal degeneration, especially in regard to the complexity of the c4 gene locus and the association of copy number variations of the C4A gene in different disease (Grassmann et al., 2016;Sekar et al., 2016).
Interestingly, Rohrer et al. (2007) suggested an important role of the alternative complement pathway in LD as photoreceptors of complement factor D-deficient mice were significantly protected from LD. The important function of the alternative pathway in retinal degeneration was also shown in CFBdeficient mice, which were protected from retinal degeneration by laser-induced choroidal neovascularization and cigarette smoke exposure (Woodell et al., 2013;Schnabolk et al., 2015). We showed in accordance with these studies that cfb mRNA is strongly and promptly upregulated in the RPE/choroid and in the retina after LD, this promotes the role of CFB and the alternative pathway in LD (Figures 2B,  3B), while we did not observe CFB protein deposition in the retina and no change in the RPE/choroid with the used antibodies ( Figure 6B and Supplementary Figure S7C). CFB is cleaved into Bb and Ba during complement activation. Therefore, further studies analyzing the function of CFB in retinal damage should focus on the detection of the CFB cleavage products, Bb and Ba, in the retina to elucidate our reported discrepancy between enhanced cfb mRNA expression and the uncorrelated CFB full-length protein detection.
The lectin pathway is the third complement activation mechanism of the complement system and microarray studies showed a changed expression for ficolin, an initiating molecule of the lectin pathway, in rats after LD (Rutar et al., 2011). Our studies revealed a low expression of mbl-a and masp-1 after LD ( Table 1, Supplementary Figure S2, and Figures 2C,D, 3C,D) and no significant change either on protein or on mRNA levels ( Figures 4B, 6C and Supplementary Figures S6C, S7F). However, a participation of the lectin pathway in retinal degeneration was proposed as it was shown that MBL and MASP-1 were systemically upregulated in late AMD-patients (Osthoff et al., 2015;Kim et al., 2016). Taking our results into account, it seems that previously reported effects of the lectin pathway on RPE cells and in a laser-induced neovascularization mouse model are either supported by systemic MBL and MASP or other lectin activating molecules like ficolin, than by locally expressed mbl-a and masp-1 (Rohrer et al., 2011;Joseph et al., 2013).
In context of complement profiling in LD, we also aimed to analyze the role of C9, the main component of the terminal complement complex. C9 had been proposed to be relevant in laser-induced choroidal neovascularization in mice (Rohrer et al., 2011;Birke et al., 2014) and had been associated with the development of AMD (Wang et al., 2010;Seddon et al., 2013). Surprisingly, the transcription of the c9 gene was, besides cfp, the only complement gene which was downregulated after LD in the retina, but immediately upregulated in the RPE/choroid (Figures 2G, 3G). This is in accordance with previously shown increased deposition of terminal complement complexes at the RPE/choroid interface in mice and human age-related retinal modifications (Tomita et al., 2005;Izawa et al., 2016;Niwa et al., 2016), but it is contrary to the decreasing protein concentration of C9 in the RPE/choroid determined by Western blots in this study ( Figure 6F and Supplementary Figure S7E). We suggest that the enhanced c9 mRNA synthesis in the RPE/choroid following LD resulted in higher concentrations of C9 protein, which was immediately integrated into membrane pores (Supplementary Figures S5C-E) and could not be purified and detected in the used Western blot protocol (Figures 4D, 6F and Supplementary Figures S6B, S7E). Therefore, the soluble C9 protein concentration was decreased following LD, but the membrane associated C9 concentration was increased after retinal damage (Tomita et al., 2005;Izawa et al., 2016;Niwa et al., 2016).
Complement activity is tightly regulated by membrane-bound and soluble complement factors. We assumed that the expression of CFP, the only known stabilizing regulator of the alternative complement pathway, would also be altered in LD. Surprisingly, cfp mRNA expression was not upregulated after LD like other tested complement factors (Figures 2H, 3H). Cfp expression has not been evaluated in previous LD studies. Indeed, cfp expression has been shown to be quite stable in vivo and in vitro in response to several stimuli such as preeclampsia or renal tubular damage (Reis et al., 2006;Buurma et al., 2012;Nagamachi et al., 2014), while it has been described to be upregulated following shear stress in endothelium (Bongrazio et al., 2003). However, our results do not automatically imply, that CFP is not involved in degeneration, as we know from other studies that CFP deposition was positive in patient eyes with AMD (Wolf-Schnurrbusch et al., 2009) and was associated with drusen-like deposition in Alzheimer's disease (Fonseca et al., 2011). Further studies have to be performed to decipher the expression pattern of CFP in more detail.
Interestingly, the activation pattern of the complement system (at protein level) mirrors the glial activation pattern. Similar to increasing C3 and C1s protein levels from 1 to 3 days post-treatment, the microglia/macrophages migrated into the subretinal space and got into close contact with photoreceptor segments 3 days after LD. Moreover C3d deposition on GFAP-positive Müller glia cells was only observed in retinae 3 days after LD. Specifically microglia/macrophages are known to express receptors for complement factors such as C3a (Rutar et al., 2011). In sum, this could imply that the complement activity by binding on Müller cells (e.g., C3b or C3d levels) triggers or modulates the microglial (e.g., C3a) response pattern upon tissue damage. This finding should be followed up in future studies.

CONCLUSION
Our results indicate a time-dependent and tissue-specific complement modulation in close association with LD in albino mice eyes which was mostly independent of the used light intensities. In the end, the complement proteins C3, C1s and C9 were identified as putative marker proteins in the LD model to monitor disease progression, as they showed valid concentration changes in a time-dependent manner compared to control mice (Figures 4C, 6A,F and Supplementary Figures S6D, S7D,E). The most significant alterations in complement protein concentration in the retina (C3) and RPE/choroid-complex (C9, C1s) after LD were observed 3 days post-treatment. The proteins were regulated in different ways: C3 and C1s were increased, but C9 was downregulated. This may indicate different functions of the proteins during retinal degeneration that need to be investigated in future studies.
Complement expression is time-dependently regulated after the initiation of cell stress in the retina and RPE/choroid. This also implies that one should monitor the eyespecific complement expression pattern after damage, when complement-manipulating reagents are tested for therapeutic reasons in the mouse eye.

AVAILABILITY OF DATA AND MATERIAL
The datasets supporting the conclusions of this article are available at the University of Regensburg Publication Server, http://doi.org/10.5283/epub.35102